Mao J.,China Pharmaceutical University |
Liu S.,China Pharmaceutical University |
Ai M.,Second Hospital of Nanjing |
Wang Z.,Nanjing University |
And 5 more authors.
Journal of Hematology and Oncology | Year: 2017
Melittin is the main effective component of bee venom and has extensive biological functions; however, serious side effects have restricted its clinical application. Preclinical and clinical studies showed that the main adverse events were allergic reaction and pain at the administration site. To decrease the toxicity, we prepared melittin nano-liposomes by encapsulating melittin with poloxamer 188 and explored the inhibitory activities on liver cancer together with biological safety. Here, we showed that melittin nano-liposomes significantly inhibited the survival of hepatocellular carcinoma (HCC) cells in vitro and prominently suppressed the growth of subcutaneous and orthotopic HCC transplantation tumors in vivo. It was important that it induced less inflammation and allergy in mice compared with melittin. Overall, melittin nano-liposomes would have a better application in HCC therapy due to its significant anti-tumor activity and better biological safety. © 2017 The Author(s).
Han W.,Shanghai JiaoTong University |
Pan H.,Shanghai JiaoTong University |
Jiang L.,Second Hospital of Nanjing |
Zou D.,Tongji University |
And 2 more authors.
Oncology Reports | Year: 2011
Major histocompatibility complex (MHC) class I molecules have been found to be downmodulated in many tumors. The antigen-processing machinery (APM) genes, especially transporters associated with antigen processing (TAP)-1 and tapasin play important roles in the processing of class I antigens. In this study, we investigated the expression of TAP-1 and tapasin in oral squamous cell carcinoma (OSCC); the result indicated significant down-regulation in the expression of these genes. Interferon (IFN)-γtreatment was applied. After the addition of IFN-γ, unexpectedly, the phosphoinositide 3-kinase (PI3K)/AKT signaling pathway was activated, which induced the proliferation of tumor cells. With the combined application of LY294002 (specific inhibitor of AKT signaling) and IFN-γ, tumor cell apoptosis was induced and the expression of TAP-1 and tapasin was still upregulated. Hence, our method is a novel and efficient approach to use IFN-γfor rescuing the cells from immunosurveillance.
Ma N.,Nanjing Southeast University |
Ma C.,Nanjing Southeast University |
Ma C.,Guangdong University of Petrochemical Technology |
Wang N.,Second Hospital of Nanjing |
And 7 more authors.
Journal of Nanoscience and Nanotechnology | Year: 2014
A novel and simple emulsifier-free emulsion polymerization technique was developed for preparation of mono-dispersed amino functionalized polymer microspheres with well defined diameters (about 400 nm). Various characterization methods demonstrated that the obtained amino microspheres had a uniform size and good dispersity which were confirmed by scanning electron microscope (SEM). Zeta potential and Fourier transform infrared spectrometer (FT-IR) demonstrated that amino groups have been successfully introduced to the microsphere surface. These functionalized microspheres have been shown to be efficient and controllable carriers capable of immobilizing and enriching monoclonal antibodies. Moreover, a newest chemiluminescent enzyme-linked immunoassay (ELISA) approach has been developed for human Hepatitis B virus surface antigen (HBsAg) detection. HBsAg was sandwiched between goat anti-HBsAg polyclonal antibody and mouse anti-HBsAg antibody. Alkaline phosphatase (ALP) conjugated horse anti-mouse immunnogloblin was used to bond with monoclonal antibody. Finally, chemiluminesent (CL) signals were recorded after adding 3-(2- spiroadamantane)-4-methoxy-4-(3-phosphoryloxy) phenyl-1,2-dioxetane (AMPPD) which was used as a chemiluminescent substrate reagent of ALP. This novel chemiluminescent ELISA assay was proved to be of excellent specificity and high sensitivity when using ALP and AMPPD luminescence systems for specific HBsAg detection. Copyright © 2014 American Scientific Publishers.
Lu Q.,Nanjing Southeast University |
Zhou Z.,Second Hospital of Nanjing |
Mei Y.,Nanjing Southeast University |
Wei W.,Nanjing Southeast University |
Liu S.,Nanjing Southeast University
Talanta | Year: 2013
This work reports a fluorescent dye insertion approach for detection of DNA damage. The capture DNA with overhanging 3'-terminus was immobilized on silicon surface to hybridize with target DNA. The intercalation of cyanine dye of thiazole orange (TO) to the double helix structure of DNA (dsDNA) allowed intense enhancement of fluorescence signal. The DNA damage with chemicals led to poor intercalation of TO into double helix structure, resulting in the decrease of the fluorescence signal. This signal decrease could be further enhanced by exonuclease III (Exo III). With this approach, the target DNA could be detected down to 47 fM. Seven chemicals were chosen as models to monitor DNA damage. The results suggested that the present strategy could be developed to detect DNA damage, to classify the damaging mechanism with chemicals and to estimate the toxic effect of chemicals. © 2013 Elsevier B.V.
Huang P.,Nanjing University |
Huang P.,HIGH-TECH |
Huang P.,Jiangsu Key Laboratory of Chinese Medicine Processing |
Tan S.,Second Hospital of Nanjing |
And 11 more authors.
Journal of Ethnopharmacology | Year: 2014
Ethnopharmacological relevance Ascending and descending theory is a core principle of traditional Chinese medicine (TCM) theories. It plays an essential role in TCM clinical applications. Some TCM medicine has specific properties, which could alter the inclination and direction of their actions. The properties of the ascending and floating process of one herbal medicine are affected by means of herb processing. Wine-processing, which is sautéing with rice wine, is one of the most popular technologies of herb processing. Wine-processing increases the inclination and direction of its actions, thereby producing or strengthening their efficacy in cleaning the upper-energizer heat. Radix scutellariae, the dried roots of Scutellaria baicalensis Georgi, is a well-known TCM used for the treatment of inflammation, pyrexia, jaundice, etc. Recently, wine-processed Radix scutellariae was normally applied in clinical studies for the treatment of upper-energizer syndrome. In order to investigate the effects of wine-processing on ascending and descending of Radix scutellariae, the comparative study of distribution of flavonoids in rat tissues of triple energizers (SanJiao-upper, middle, lower jiao) after oral administration of crude and wine-processed Radix scutellariae aqueous extracts was carried out. Materials and methods The rats were randomly assigned to two groups and orally administered with crude and wine-processed Radix scutellariae aqueous extracts, respectively. At different pre-determined time points after administration, the concentrations of compounds in rat tissue homogenate were determined, and the main tissue pharmacokinetic parameters were investigated. Tissue pharmacokinetic parameters including AUC0-t, t1/2, Tmax and Cmax were calculated using DAS 2.0. An unpaired Student t-test was used to compare the differences in tissue pharmacokinetic parameters between the two groups. All the results were expressed as arithmetic mean±S.D. Results The parameters of Cmax and AUC0-t of some flavonoids in wine-processed Radix scutellariae were remarkably increased (p<0.05, p<0.01, p<0.001) in the rat upper-energizer tissues (lung and heart) compared with those of the crude group. However, in the rat middle- and lower-energizer tissues (spleen, liver and kidney), the C max and AUC0-t of some flavonoids were significantly decreased (p<0.05, p<0.01) compared with the crude group. The main explanation for these differences seems to the effects of wine-processing on ascending and descending theory. Conclusions All of these differences in the distribution of triple energizers after oral administration of crude and wine-processed Radix scutellariae aqueous extracts may lead to the increase of efficacy on the upper-energizer tissues and were in compliance with the ascending and descending theory. Therefore, wine-processing was recommended when Radix scutellariae was used for cleaning the upper-energizer heat and humidity. The obtained knowledge can be used to evaluate the impact of these differences on the efficacy of both the drugs in clinical applications and might be helpful in explaining the effects of wine-processing on ascending and descending theory. © 2014 Elsevier Ireland Ltd. All rights reserved.
Ji H.,U.S. Center for Disease Control and Prevention |
Gu Q.,Nanjing Drum Tower Hospital |
Chen L.-L.,U.S. Center for Disease Control and Prevention |
Xu K.,U.S. Center for Disease Control and Prevention |
And 22 more authors.
PLoS ONE | Year: 2014
Background: A novel avian influenza A (H7N9) virus has caused great morbidity as well as mortality since its emergence in Eastern China in February 2013. However, the possible risk factors for death are not yet fully known. Methods and Findings: Patients with H7N9 virus infection between March 1 and August 14, 2013 in Jiangsu province were enrolled. Data were collected with a standard form. Mean or percentage was used to describe the features, and Fisher's exact test or t-test test was used to compare the differences between fatal and nonfatal cases with H7N9 virus infection. A total of 28 patients with H7N9 virus infection were identified among whom, nine (32.1%) died. The median age of fatal cases was significant higher than nonfatal cases (P<0.05). Patients with older age were more strongly associated with increased odds of death (OR = 30.0; 95% CI, 2.85-315.62). Co-morbidity with chronic lung disease and hypertension were risk factors for mortality (OR = 14.40; 95% CI, 1.30-159.52, OR = 6.67; 95% CI, 1.09-40.43, respectively). Moreover, the presence of either bilateral lung inflammation or pulmonary consolidation on chest imaging on admission was related with fatal outcome (OR = 7.00; 95%CI, 1.10-44.61). Finally, dynamic monitoring showed that lymphopenia was more significant in fatal group than in nonfatal group from day 11 to week five (P<0.05). The decrease in oxygenation indexes were observed in most cases and more significantly in fatal cases after week three (P<0.05), and the value of nearly all fatal cases were below 200 mmHg during our evaluation period. Conclusions: Among cases with H7N9 virus infection, increased age accompanied by co-morbidities was the risk of death. The severity of lung infection at admission, the persistence of lymphocytopenia, and the extended duration of lower oxygenation index all contributed to worsened outcomes of patients with H7N9 virus infection. © 2014 Ji et al.
Zhang B.,Wuxi Infectious Diseases Hospital |
Hu M.,Wuxi Infectious Diseases Hospital |
Zhang P.,Nanjing Medical University |
Cao H.,Wuxi Infectious Diseases Hospital |
And 3 more authors.
Brazilian Journal of Medical and Biological Research | Year: 2013
Primary biliary cirrhosis (PBC) is a chronic and slowly progressive cholestatic liver disease of autoimmune etiology. A number of questions regarding its etiology are unclear. CD4+CD25+regulatory T cells (Tregs) play a critical role in self-tolerance and, for unknown reasons, their relative number is reduced in PBC patients. B-cell-activating factor (BAFF) is a key survival factor during B-cell maturation and its concentration is increased in peripheral blood of PBC patients. It has been reported that activated B cells inhibit Treg cell proliferation and there are no BAFF receptors on Tregs. Therefore, we speculated that excessive BAFF may result in Treg reduction via B cells. To prove our hypothesis, we isolated Tregs and B cells from PBC and healthy donors. BAFF and IgM concentrations were then analyzed by ELISA and CD40, CD80, CD86, IL-10, and TGF-b expression in B cells and Tregs were measured by flow cytometry. BAFF up-regulated CD40, CD80, CD86, and IgM expression in B cells. However, BAFF had no direct effect on Treg cell apoptosis and cytokine secretion. Nonetheless, we observed that BAFF-activated B cells could induce Treg cell apoptosis and reduce IL-10 and TGF-b expression. We also showed that BAFF-activated CD4+T cells had no effect on Treg apoptosis. Furthermore, we verified that bezafibrate, a hypolipidemic drug, can inhibit BAFF-induced Treg cell apoptosis. In conclusion, BAFF promotes Treg cell apoptosis and inhibits cytokine production by activating B cells in PBC patients. The results of this study suggest that inhibition of BAFF activation is a strategy for PBC treatment.
He L.-W.,Nanjing University |
Liu H.-Q.,Second Hospital of Nanjing |
Chen Y.-Q.,Nanjing University |
Yang J.-Y.,Nanjing University |
And 2 more authors.
Molecules | Year: 2014
Radix isatidis (Banlangen), a famous traditional Chinese medicine, has been used for thousands of years in China due to its anti-viral activity. Through our research, we inferred that the anti-viral activity of Radix isatidis depended on the water-soluble part. Among the components of this extract, the isoquinoline derivative 1 was isolated for the first time and has shown better anti-viral activity than other constituents. In this study, to solve the problem of sourcing sufficient quantities of compound 1, a total synthesis route is described, and several analogues are also evaluated for their anti-viral activities. Among them, compound 8 shown potent anti-viral activity with an IC50 value of 15.3 μg/mL. The results suggested that isoquinoline derivatives possessed potent anti-viral activity and are worthy further development. © 2014 by the authors licensee MDPI Basel Switzerland.
Chen Y.,Nanjing University |
Liu H.,Second Hospital of Nanjing |
Xu S.,Nanjing University |
Wang T.,Nanjing University |
Li W.,Nanjing University
MedChemComm | Year: 2015
Prostaglandin E2 (PGE2) is an important substance in diverse human organs and regulates a variety of physiological and pathological processes, such as inflammation, fever, pain and cancer. Microsomal prostaglandin E2 synthase-1 (mPGES-1), a glutathione (GSH)-dependent enzyme, can induce the generation of PGE2. Since the 1990s, scientists have been studying the biochemistry and pathophysiology of mPGES-1, proposing it to be a promising target for the treatment of PGE2 metabolic abnormalities. Nevertheless, selective mPGES-1 inhibitors have not been available in clinics up to now, and studies on mPGES-1 are rare. Unlike non-steroidal anti-inflammatory drugs (NSAIDs), mPGES-1 inhibitors can reduce PGE2 production without affecting the generation of other PGs and thromboxane (Tx), indicating fewer side effects of mPGES-1 inhibitors. Recently, many chemical substances obtained from natural plants, human endogenous substances and chemical synthesis have been demonstrated to have strong inhibitory effects on mPGES-1. Here, we mainly review studies on mPGES-1 biology and mPGES-1 inhibitors along with their chemistry, biological activity and docking algorithm of computer-aided molecular design in the past decade. © Royal Society of Chemistry 2015.
Yu X.-Y.,Second Hospital of Nanjing |
An J.-G.,Second Hospital of Nanjing |
Hua Y.-H.,Second Hospital of Nanjing |
Li Z.-H.,Second Hospital of Nanjing |
Yan N.,Second Hospital of Nanjing
Journal of Clinical Dermatology | Year: 2015
Objective: To investigate the relationship between gender and depression incidence in patients with psoriasis in China. Methods: Two hundred and forty six, aged ≥ 18-year-old, outpatients with psoriasis were surveyed using center for epidemiologic studies depression scale (CES-D). Results: 31.43% of the patients had obvious depression symptoms. Although the social status and work status were different between the males and females, depression symptoms did not vary with gender. Conclusion: In China, psoriatic patients showed no gender differences in depression symptoms.