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Guo S.-L.,Shanghai University | Guo S.-L.,Nanjing Regenerative Medicine Engineering and Technology Research Center | Zhang Z.-Y.,Shanghai University | Xu Y.,Nanjing Regenerative Medicine Engineering and Technology Research Center | And 6 more authors.
Stem Cells International | Year: 2015

Effective repair of peripheral nerve defects is difficult because of the slow growth of new axonal growth. We propose that "neural-like cells" may be useful for the protection of peripheral nerve destructions. Such cells should prolong the time for the disintegration of spinal nerves, reduce lesions, and improve recovery. But the mechanism of neural-like cells in the peripheral nerve is still unclear. In this study, bone marrow-derived neural-like cells were used as seed cells. The cells were injected into the distal end of severed rabbit peripheral nerves that were no longer integrated with the central nervous system. Electromyography (EMG), immunohistochemistry, and transmission electron microscopy (TEM) were employed to analyze the development of the cells in the peripheral nerve environment. The CMAP amplitude appeared during the 5th week following surgery, at which time morphological characteristics of myelinated nerve fiber formation were observed. Bone marrow-derived neural-like cells could protect the disintegration and destruction of the injured peripheral nerve. © 2015 Shi-lei Guo et al.

Xu Y.,Nanjing Regenerative Medicine Engineering and Technology Research Center | Xu Y.,Shanghai University | Guo S.,Nanjing Regenerative Medicine Engineering and Technology Research Center | Guo S.,Shanghai University | And 5 more authors.
Stem Cells International | Year: 2016

Background. Mesenchymal stem cells are the most commonly used seed cells in biomedical research and tissue engineering. Their secretory proteins have also been proven to play an important role in tissue healing. Methods. We isolated adipose stem cells and placental stem cells and performed analysis examining characteristics. The secretory proteins were extracted from conditioned medium and analyzed by MALDI-TOF/TOF. The antiaging effect of conditioned mediums was evaluated by the results of facial skin application. Results. Adipose stem cells and placental stem cells were found to be very similar in their surface markers and multipotency. The specific proteins secreted from adipose stem cells were more adept at cell adhesion, migration, wound healing, and tissue remodeling, while the proteins secreted by placental stem cells were more adept at angiogenesis, cell proliferation, differentiation, cell survival, immunomodulation, and collagen degradation. While these two types of conditioned medium could improve the facial index, the improvement of Melanin index after injection of the adipose stem cell conditioned medium was much more significant. Conclusion. The results suggest that the secreted proteins are ideal cell-free substances for regeneration medicine, especially in the antiaging field. Copyright © 2016 Yan Xu et al.

Huang J.,Nanjing University | Huang J.,Nanjing Southeast University | Huang J.,Nanjing Regenerative Medicine Engineering and Technology Research Center | Wang S.,Nanjing Medical University | And 7 more authors.
Tissue and Cell | Year: 2011

A hybrid hydrogel (PP) composed of Polomaxer-407 (PO) and octapeptide with amino acid sequence of KFEFKFEF (PE) was prepared to make a scaffold material incorporating PO's high and tunable mechanical strength and integrity with PE's superior bioactivity. Human adipose-derived mesenchymal stem cells (hASCs) were encapsulated into PE, PO and PP hydrogels respectively and injected subcutaneously at the dorsal neck area of nude mice. Adipose-like tissue regeneration was only observed in the mice injected with cell-encapsulated PP hydrogel. No adipose regeneration was found in the mice injected with PO or PE. Immunohistochemistry analysis with mouse anti-human nuclei monoclonal antibody demonstrated that the cells in the regenerated adipose-like tissue was originated from the injected hASCs. The growth of blood capillaries indicated that the regenerated adipose-like tissue was living tissue. In addition, human-originated cells were also found in nude mice skin. These cells were positive with mouse anti-human cells keratin antibody, suggesting that the injected hASCs migrated to the skin and differentiated into epithelial cells in vivo. © 2011 Elsevier Ltd.

Xu Y.,Nanjing Medical University | Zhang J.-A.,Nanjing Medical University | Xu Y.,Nanjing Regenerative Medicine Engineering and Technology Research Center | Guo S.-L.,Nanjing Regenerative Medicine Engineering and Technology Research Center | And 5 more authors.
Stem Cells and Development | Year: 2015

Photoaging of skin occurs partially due to decreased synthesis and increased degradation of dermal collagen. Antiphotoaging therapy aims to counteract these effects. This study aimed to investigate whether secretory factors from dedifferentiated adipocytes (DAs) could alleviate photoaging in human dermal fibroblasts (HDFs) and in mice and to clarify the underlying mechanism. DAs were acquired and verified based on cellular biomarkers and multilineage differentiation potential. The concentrations of several cytokines in conditioned medium from DAs (DA-CM) were determined. In vivo pathological changes, collagen types I and III, and matrix metalloproteinase (MMP)-1 and -3 were evaluated following the injection of 10-fold concentrated DA-CM into photoaged mice. In vitro, the effect of DA-CM on stress-induced premature senescence in HDFs was investigated by 5-ethynyl-2'-deoxyuridine (EdU) staining and β-galactosidase staining. The influence of DA-CM and transforming growth factor-β1 (TGF-β1) on the secretion of collagen types I and III, MMP-1, and MMP-3 in HDFs was evaluated by ELISA. In vivo, we found that subcutaneously injected 10-fold concentrated DA-CM increased the expression of collagen types I and III. In vitro, DA-CM clearly mitigated the decreased cell proliferation and delayed the senescence status in HDFs induced by ultraviolet B (UVB). HDFs treated with DA-CM exhibited higher collagen types I and III secretion and significantly lower MMP-1 and MMP-3 secretion. The TGF-β1-neutralizing antibody could partially reduce the recovery effect. Our results suggest that DAs may be useful for aging skin and their effects are mainly due to secreted factors, especially TGF-β1, which stimulate collagen synthesis and alleviate collagen degradation in HDFs. © Copyright 2015, Mary Ann Liebert, Inc. 2015.

Zhou B.-R.,Nanjing Medical University | Zhang T.,Nanjing Medical University | Bin Jameel A.A.,Nanjing Medical University | Xu Y.,Nanjing Medical University | And 5 more authors.
Journal of Cosmetic and Laser Therapy | Year: 2016

Objective: To evaluate the effects of conditioned medium of adipose-derived stem cells (ADSC-CM) on efficacy and side effects after fractional carbon dioxide laser resurfacing (FxCR) when treating subjects with facial atrophic acne scars or with skin rejuvenation needs. Materials and methods: Twenty-two subjects were enrolled in the study and divided into two groups. Nine subjects were included in skin rejuvenation group and thirteen subjects were included in acne scar group, and all subjects underwent three sessions of FxCR. ADSC-CM was applied on FxCR site of one randomly selected face side. Evaluations were done at baseline, 1 week after first treatment, and 1 month after each treatment. The outcome assessments included subjective satisfaction scale; blinded clinical assessment; and the biophysical parameters of roughness, elasticity, skin hydration, transepidermal water loss (TEWL), and the erythema and melanin index. Biopsies taken from one subject in skin rejuvenation group were analyzed using hematoxylin and eosin, Masson's Trichrome, and Gomori's aldehyde fuchsin staining. Results: ADSC-CM combined with FxCR increased subject satisfaction, elasticity, skin hydration, and skin elasticity and decreased TEWL, roughness, and the melanin index in both acne scars and skin rejuvenation groups. Histologic analysis showed that ADSC-CM increased dermal collagen density, elastin density, and arranged them in order. Conclusion: ADSC-CM with FxCR is a good combination therapy for treating atrophic acne scars and skin rejuvenation. Trial registration: JSPH2012-082 – Registered 14 Feb 2012 © 2016 Taylor & Francis Group, LLC.

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