Ji W.-W.,Nanjing NormalLuniversity |
Chen T.,Nanjing NormalLuniversity |
Sang Q.,Nanjing NormalLuniversity |
Dai Y.-J.,Nanjing NormalLuniversity |
And 2 more authors.
Journal of Ecology and Rural Environment | Year: 2010
A strain of filamentous fungus capable of degrading acetamiprid, an insecticide of chloronicotinyl neonicotinoid series, was isolated from soil and named as Strain IM -3. IM -3 was identified as Penicillium oxalicum by morphology and 18S rDNA sequence analysis. In the medium of mineral salts, incubated for 14 days, P. oxalicum degraded 41. 6% of AAP and 14. 1% of imidacloprid ; but nil of thiacloprid and nitenpyram. HPLC and LC-MS/MS analysis revealed that the metabolic pathway of AAP by P. oxalicum IM - 3 was N-demethylation of AAP to form IM 2 - 1 and oxidative cleavage of cyanoimine moiety to form IM 1 -3.