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Zhang X.,Nanjing University | Zheng X.,Nanjing Municipal Hospital of TCM | Liu X.,Nanjing University | Yu H.,Nanjing University | And 3 more authors.
Asian Journal of Chemistry | Year: 2015

The aim of this study is to establish the fingerprint of Coptis chinensis by HPLC. HPLC analysis was carried out with Kromasil C18column (4.6 × 250 mm) at the wavelength of 300 nm at 30°C. Acetonitrile-0.01 mol L-1 KH2PO4 solution (gradient elution) was used as the mobile phase at the flow rate of 1 mL min-1. Different Coptis chinensis samples had ten common peaks with the similarity not less than 95%. The areas of non-common peaks accounted for less than 10% of the total peak area. This method was highly stable and repeatable and the established HPLC fingerprint provided evidence for the quality control of Coptis chinensis. © 2015, Chemical Publishing Co. All rights reserved. Source


Qiu P.,Nanjing University | Qiu P.,Zhejiang Chinese Medical University | Li X.,Nanjing University | Kong D.-S.,Nanjing Municipal Hospital of TCM | And 3 more authors.
Evidence-based Complementary and Alternative Medicine | Year: 2015

Our previous study indicated that herbal SGR formula partially attenuates ethanol-induced fatty liver, but the underlying mechanisms remain unclear. In the present study, mice were pretreated with SGR (100 and 200 mg/kg/d bw) for 30 d before being exposed to ethanol (4.8 g/kg bw). The biochemical indices and histopathological changes were examined to evaluate the protective effects and to explore potential mechanisms by investigating the adiponectin, tumor necrosis factor-α (TNF-α), peroxisome proliferators-activated receptor-α (PPAR-α), sterol regulatory element binding protein-1c (SREBP-1c), adenosine monophosphate-activated protein kinase (AMPK), and so forth. Results showed that SGR pretreatment markedly inhibited acute ethanol-induced liver steatosis, significantly reduced serum and hepatic triglyceride (TG) level, and improved classic histopathological changes. SGR suppressed the protein expression of hepatic SREBP-1c and TNF-α and increased adiponectin, PPAR-α, and AMPK phosphorylation in the liver. Meanwhile, acute toxicity tests showed that no death or toxic side effects within 14 days were observed upon oral administration of the extracts at a dose of 16 g/kg body wt. These results demonstrate that SGR could protect against acute alcohol-induced liver steatosis without any toxic side effects. Therefore, our studies provide novel molecular insights into the hepatoprotective effect of SGR formula, which may be exploited as a therapeutic agent for ethanol-induced hepatosteatosis. © 2015 Ping Qiu et al. Source


Li S.M.,Nanjing Municipal Hospital of TCM
Zhongguo gu shang = China journal of orthopaedics and traumatology | Year: 2010

OBJECTIVE: To evaluate the treatment effect of three and four-part fractures of proximal humerus in elder patients using indirect reduction combined with locking proximal humeral plate (LPHP) fixation. METHODS: From November 2004 to May 2008, 24 cases of proximal humeral three and four-part fracture were treated with percutaneous plate fixation using LPHP included 6 males and 18 females with an average age of 70 years old ranging from 55 to 88 years. The surgery was performed through antrolateral transdeoltoid approach. After extra articular capsule indirect reduction, the plate was inserted through the incision, and then an incision was made distal to previous incision to expose distal plate. The proximal fragments were fixed with 4 to 6 screws, and distal fragments were fixed with 3 screws. Shoulder exercises were performed at the 2nd to 3rd day after operation. Two weeks after operation, active shoulder exercise was done, which was gradually intensified 3 weeks after operation. The functional outcomes of the shoulder were assessed according to Constant score. RESULTS: All the fractures united clinically and radiologically. The mean healing time was 10.5 weeks (8 to 21 weeks). The mean Constant score was 81.6 points (49 to 92 points). A total of 20 patients had excellent or satisfactory result, with only one scored as poor. CONCLUSION: The technique of extra articular capsule indirect reduction combined LPHP internal fixation has the advantages of stable, easy to operate, less vascular damage and so on. It can effectively treat the proximal humerus three-and four-part fractures, especially to elder patients. Source


Zong H.,Nanjing Municipal Hospital of TCM | Zhang Y.,Nanjing Municipal Hospital of TCM | You Y.,Zhangjiagang Wandong Anorectal Hospital | Cai T.,Nanjing Municipal Hospital of TCM | Wang Y.,Nanjing Municipal Hospital of TCM
Medical Oncology | Year: 2015

ATP citrate lyase (ACLY) is responsible for the conversion of cytosolic citrate into acetyl-CoA and oxaloacetate, and the first rate-limiting enzyme involved in de novo lipogenesis. Recent studies have demonstrated that inhibition of elevated ACLY results in growth arrest and apoptosis in a subset of cancers; however, the expression pattern and underlying biological function of ACLY in pancreatic ductal adenocarcinoma (PDAC) remains unclear. In the current study, overexpressed ACLY was more commonly observed in PDAC compared to normal pancreatic tissues. Kaplan–Meier survival analysis showed that high expression level of ACLY resulted in a poor prognosis of PDAC patients. Silencing of endogenous ACLY expression by siRNA in PANC-1 cells led to reduced cell viability and increased cell apoptosis. Furthermore, significant decrease in glucose uptake and lactate production was observed after ACLY was knocked down, and this effect was blocked by 2-deoxy-d-glucose, indicating that ACLY functions in the Warburg effect affect PDAC cell growth. Collectively, this study reveals that suppression of ACLY plays an anti-tumor role through decreased Warburg effect, and ACLY-related inhibitors might be potential therapeutic approaches for PDAC. © 2015, Springer Science+Business Media New York. Source


Li J.-Y.,Nanjing Municipal Hospital of TCM | Zhao Y.,Nanjing Municipal Hospital of TCM | Zhang Z.-N.,Nanjing Municipal Hospital of TCM
Journal of Clinical Neurology | Year: 2010

Objective: To compare the curative effects between Tongnaohuoluo acupuncture therapy and the conventional therapy on acute cerebral infarction (ACI), and the best therapeutic time window of Tongnaohuoluo acupuncture therapy on ACI. Methods: By the methods of multi-centered, randomized and controlled, 145 ACI patients (acupuncture therapy group) according course were divided into <6 h sub-group, 6-48 h sub-group and > 48 h sub-group. Based on conventional treatment, Tongnaohuoluo acupuncture therapy was given once daily for 14 d. The score of National Institute of Health stroke scale (NIHSS) , Barthel index (B1) pre and post treatment were evaluated. Ninety d after treatment, the curative effects were assessed and compared with other 125 ACI patients who only taken conventional therapy (conventional therapy group). Results: After treatment, the total effective rates in each acupuncture therapy sub-group (96.4%, 90.9%, 80.4%) were significant higher 4han those in each conventional therapy sub-group (86.7%, 75.0%, 58.3%) (all P<0.01); and in acupuncture therapy group, which in <6 h sub-group was significantly higher than 6-48 h and >48 h sub-groups(P <0.05-0.01). Compared with each conventional sub-group, NIHSS scores in the each acupuncture sub-group was, significantly lower and BI was significantly higher(P < 0.05-0.01); and in acupuncture therapy group, which in <6 h sub-group were superior to 6-48 h and >48 h sub-groups(P <0.05-0.01). Conclusions: Tongnaohuoluo acupuncture therapy for ACI has remarkable efficacious. The best time of this therapy is within 6 h after ACI onset. Source

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