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Alexander R.E.,Indiana University | Lopez-Beltran A.,University of Cordoba, Spain | Montironi R.,Marche Polytechnic University | MacLennan G.T.,Case Western Reserve University | And 10 more authors.
Histopathology | Year: 2012

Aims: To determine whether KRAS mutations occur in primary bladder adenocarcinoma. Methods and results: Twenty-six cases of primary urinary bladder adenocarcinoma were analysed. DNA was extracted from formalin-fixed, paraffin-embedded tissue and amplified with shifted termination assay technology, which recognizes wild-type or mutant target sequences and selectively extends detection primers with labelled nucleotides. A mutation in KRAS was found in three (11.5%) of 26 primary bladder adenocarcinomas. Two of these three cases exhibited a G13D mutation, whereas the remaining case contained a mutation in G12V. None of the ten cases of urothelial carcinoma with glandular differentiation displayed KRAS mutation. Colonic adenocarcinoma contained a KRAS mutation in 18 (33%) of 55 cases. There was no distinct difference with regard to grade, stage or outcome according to the limited clinicopathological data available. However, the two youngest patients, aged 32 and 39years, in our study group, with a mean population age of 61years, were found to have mutations in KRAS. Conclusions: KRAS mutations are present in a small subset of primary urinary bladder adenocarcinomas. Future clinical trials for treatment of bladder adenocarcinoma, employing targeted therapies similar to those used for treatment of colon cancer, may also benefit from the predictive implications of KRAS mutational testing. © 2012 Blackwell Publishing Limited. Source

Wang Y.,Nanjing University | Zhou J.,Wuxi Peoples Hospital | Huang W.-B.,Nanjing Medical College Affiliated Nanjing First Hospital | Rao Q.,Nanjing University | And 2 more authors.
International Journal of Surgical Pathology | Year: 2011

Calcifying nested stromal-epithelial tumor (CNSET) of the liver is extremely rare. This tumor is characterized by nests of epithelial and spindle cells, an associated desmoplastic stroma, as well as variable calcifications and ossifications. Only 24 cases have been reported in the literature whereas none has been reported in Asian descendants. The authors report the first case of CNSET in a 34-year-old Asian woman and provide detailed histological and clinical follow-up data. Compared with those reported earlier, the present case with a history of oral contraceptive use displayed most typical features and the oldest age of onset. A retrospective study was made and the characteristics of CNSET were summarized. © The Author(s) 2011. Source

Huang W.,Nanjing Medical College Affiliated Nanjing First Hospital | Zhao Y.,Nanjing Medical College Affiliated Nanjing First Hospital | Zhao J.,Nanjing Medical College Affiliated Nanjing First Hospital | Huang Y.,Nanjing Medical College Affiliated Nanjing First Hospital | And 2 more authors.
Pathology Research and Practice | Year: 2010

Endometrioid carcinoma is a common tumor of the female genital tract, mainly affecting the uterus corpus and ovary. In the fallopian tube, endometrioid carcinoma is relatively uncommon. The female adnexal tumor of probable wolffian origin (FATWO), although occasionally seen elsewhere, is most typically encountered in the broad ligament. Endometrioid carcinoma of the fallopian tube resembling FATWO is a rare type of endometrioid carcinoma, and only 20 cases have been reported to date. Here, we report a case of endometrioid carcinoma of the fallopian tube resembling FATWO and review the literature. A 67-year-old woman presented with a history of prolapse for about one month outside the vulva. Physical examination and pelvic ultrasonography of the patient revealed a streak mass covering the left ovary. A total hysterectomy and a bilateral salpingo-oophorectomy were performed. The tumor was confined to the tube and strongly resembled FATWO. Immunohistochemically, the tumor cells were positive for AE1/AE3, epithelial membrane antigen (EMA), and p16, but negative for CD10, inhibin-α, calretinin, vimentin, myogenin, and desmin, CD99,CgA, and Syn. We review the previously published cases of endometrioid carcinomas of the fallopian tube resembling FATWO, and discuss the principal differential diagnosis of the tumor in the fallopian tube. © 2009 Elsevier GmbH. All rights reserved. Source

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