Zhou J.-Y.,Soochow University of China |
Li X.-Q.,Nanjing Medical College |
Xu X.-T.,Soochow University of China |
Wang L.-L.,Soochow University of China |
And 2 more authors.
Tumor | Year: 2010
Objective: To investigate the inhibitory effects of celecoxib, a selective cyclooxygenase 2 (COX-2) inhibitor, on the proliferation and migration of human glioblastoma SHG-44 cells in vitro. Methods: The human glioblastoma cell lines SHG-44 were treated with different concentrations of celecoxib (30, 50, 100, and 150 μmol/L) for 24 and 48 h. The cells without celecoxib treatment were as control group. Cell morphologic changes were observed using optical microscopy. MTT assay was performed to determine the effects of celecoxib on the proliferation of SHG-44 cells. The migration ability of SHG-44 cells was measured using wound healing assay. The content of matrix metalloproteinase 2 (MMP-2) in the culture supernatant was detected with ELISA. Results: Celecoxib inhibited the growth of SHG-44 cells in concentration-and time-dependent manners. The migration ability of SHG-44 cells in celecoxib treated group was decreased compared with control group. The decreasing degree was more significant with the increase of the concentration of celecoxib. ELISA found that the level of MMP-2 in the supernatant of celecoxib-treated cells was significantly decreased compared with that of control group (P < 0.05). Conclusion: Celecoxib inhibits the proliferation and migration of human glioblastoma cell lines SHG-44 in a concentration-and time-dependent manner in vitro. Celecoxib may inhibit the migration ability of the human glioblastoma cell lines SHG-44 by suppressing MMP-2 secretion.