Nanjing Longliang Biological Science and Technology Ltd Company

Nanjing, China

Nanjing Longliang Biological Science and Technology Ltd Company

Nanjing, China
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Li Z.,Nanjing Southeast University | Li Z.,Nanjing University of Posts and Telecommunications | Li Z.,Nanjing Longliang Biological Science and Technology Ltd Company | Yang H.,Nanjing Southeast University | And 10 more authors.
Journal of Biomedical Nanotechnology | Year: 2013

For reducing the steric hindrance and nonspecific binding of the target DNA, the dextran was used as molecular arms to be immobilized on the surface of magnetic nanoparticles (MNPs). Magnetic separation was used in preparation of dextran- MNPs (DMNPs). Aspartic acid and aminated DNA probe were successively modified on the dextran immobilized on the surface of MNPs. These probe-DMNPs were successfully applied to detect biotin-labeled PCR product of E. coli O157:H7 genome by hybridization. Then the complexes were bonded with streptavidin-modified alkaline phosphatase (ALP-SA). Finally the chemiluminescent signals were detected by adding 3-(2-spiroadamantane)-4- methoxy-4- (3-phosphoryloxy) phenyl-1,2-dioxetane (AMPPD). The results showed that this method had a good specificity, and higher sensitivity than that when only MNPs were used as solid carriers. Copyright © 2013 American Scientific Publishers All rights reserved.


Tang Y.,Nanjing Southeast University | Tang Y.,Hunan Institute of Engineering | Li Z.,Nanjing Southeast University | Li Z.,Nanjing Longliang Biological Science and Technology Co. | And 12 more authors.
Journal of Biomedical Nanotechnology | Year: 2013

A rapid detection method of Pseudomonas aeruginosa based on magnetic separation and chemiluminescence was developed in this paper. Magnetic nanoparticles (MNPs) were prepared by solvothermal method with PEG-4000 as a surfactant, and then were modified. The prepared MNPs present a uniform morphology and good dispersion. The sizes of MNPs can be controlled by adjusting the dosage of FeCl3 · 6H2O. The obtained particles were characterized with Scanning electron microscope (SEM), Transmission electronic microscopy (TEM) and Fourier transform infrared (FTIR). The biotin-dUTP-labeled DNA fragments of gyrB gene were amplified by polymerase chain reaction (PCR), and Pseudomonas aeruginosa was successfully detected with detection limit as low as 7.5 fM of gyrB fragments. Copyright © 2013 American Scientific Publishers All rights reserved.


Liu B.,Nanjing Southeast University | Jia Y.,Nanjing Southeast University | Ma M.,Nanjing Southeast University | Li Z.,Nanjing Southeast University | And 15 more authors.
Journal of Biomedical Nanotechnology | Year: 2013

Single-nucleotide polymorphism (SNP) was one-base variations in DNA sequence that can often be helpful to find genes associations for hereditary disease, communicable disease and so on. We developed a high throughput SNP detection system based on magnetic nanoparticles (MNPs) separation and dual-color hybridization or single base extension. This system includes a magnetic separation unit for sample separation, three high precision robot arms for pipetting and microtiter plate transferring respectively, an accurate temperature control unit for PCR and DNA hybridization and a high accurate and sensitive optical signal detection unit for fluorescence detection. The cyclooxygenase-2 gene promoter region -765G > C polymorphism locus SNP genotyping experiment for 48 samples from the northern Jiangsu area has been done to verify that if this system can simplify manual operation of the researchers, save time and improve efficiency in SNP genotyping experiments. It can realize sample preparation, target sequence amplification, signal detection and data analysis automatically and can be used in clinical molecule diagnosis and high throughput fluorescence immunological detection and so on. Copyright © 2013 American Scientific Publishers All rights reserved.


He N.,Nanjing Southeast University | He N.,Hunan University of Technology | Wang F.,Nanjing Southeast University | Ma C.,Nanjing Southeast University | And 8 more authors.
Journal of Biomedical Nanotechnology | Year: 2013

Molecular detection of HBV has a significant impact on prognosis and therapy of the disease. In this paper, a sensitive nucleic acid detection method of HBV was established taking advantage of magnetic nanoparticles (MNPs), chemiluminescence (CL) and polymerase chain reaction (PCR). HBV-DNA was extracted from hepatitis B positive human blood samples using MNPs adsorption method and biotin was labeled on the DNA segment after base insertion of bintin-dUTP in PCR. The biotinylated DNA segment was captured by amino probe immobilized on carboxyl MNPs and was detected by the chemiluminescence system of alkaline phosphatase catalyzing 3-(2'- spiroadamantane) -4-methoxy -4-(3''-phosphoryloxy) phenyl-1, 2-dioxetane. Different concentrations of HBV-DNA were detected under the optimized experiment conditions and the relevant CL intensity were obtained, which provided a novel research or clinic diagnosis method for the quantification detection of HBV-DNA. Copyright © 2013 American Scientific Publishers All rights reserved.


Liu B.,Nanjing Southeast University | Li Z.,Nanjing Southeast University | Li Z.,Yangtze University | Chen H.,Nanjing Southeast University | And 5 more authors.
Journal of Biomedical Nanotechnology | Year: 2012

With the wide application of nanomaterials in biomedical detection in recent years, hybridization methods which use nanoparticles as solid phase hybridization carriers have emerged. However, commercial equipments, such as conventional thermal cyclers and hybridization ovens are usually not appropriate for DNA hybridization on the surface of nanoparticles. We designed and improved a total temperature micro-volume blended incubating and hybridizing apparatus (TTMHA), which can be used for blending and suspending of nanoparticles in a small volume and liquid phase environment. This device highlights the mechanical rotation structure which can not only provide a uniform temperature field, but also makes the liquid flow fully in the reaction system and improves DNA hybridization efficiency significantly. A complex PID control algorithm, including Bang-Bang control and Fuzzy-PID control algorithm, was applied in this research project to improve the control accuracy and stability. Furthermore, a model detection experiment using ssDNA (single strand DNA) sequence was conducted in thermal cyclers and TTMHA respectively to verify the optimal hybridization efficiency of the TTMHA. Copyright © 2012 American Scientific Publishers All rights reserved.

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