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Tang X.-L.,Nanjing University of Technology | Lin B.-L.,Nanjing University of Technology | Cui S.,Nanjing University of Technology | Zhang X.,Nanjing University of Technology | And 4 more authors.
RSC Advances | Year: 2016

In this paper, the preparation of Fe3O4/PTX/HSA nanoparticles with magnetic resonance imaging (MRI) and slow release functionality by a self-assembly method is presented. Firstly, hydrophobic Fe3O4 nanoparticles with an average size of 10 nm are synthesized by thermal decomposition of an iron-oleate complex and then modified and stabilized by hydrophobic paclitaxel (PTX). Lastly, Fe3O4/PTX nanoparticles with a 3 nm PTX shell on the Fe3O4 surface are loaded into human serum albumin (HSA) to form uniform Fe3O4/PTX/HSA nanoparticles by PTX-HSA interaction after breaking the disulfide bond and unfolding hydrophobic region of HSA. The novel PTX modified Fe3O4/PTX nanoparticles have good dispersity in ethanol and strong binding capacity with HSA, which can be homogenously dispersed in HSA matrices to form novel Fe3O4/PTX/HSA nanoparticles with a pie structure by the self-assembly method. The resulting Fe3O4/PTX/HSA nanoparticles with a high saturation magnetization value of 10.2 emu g-1, good T2 imaging functionality and excellent ability to cross the cell membrane have been demonstrated by magnetization curves, in vitro MRI and cellular uptake. Furthermore, the in vitro antitumor ability of the system has also been evaluated. © The Royal Society of Chemistry 2016.

Zhou J.,Nanjing Jiangbei Peoples Hospital | Zhang H.-J.,Jiangsu Province Peoples Hospital
World Chinese Journal of Digestology | Year: 2015

AIM: To investigate the expression of potassium channel tetramerization domain-containing 12 (KCTD-12) protein in gastrointestinal stromal tumors (GISTs) of the stomach and its clinical significance. METHODS: The expression of KCTD-12 was immunohistochemically examined in 39 gastric GIST tissues, and its correlations with the clinicopathological parameters, National Institutes of Health (NIH) risk classification and Ki-67 labeling index were analyzed. Univariate and Cox multivariate analyses were used to evaluate the factors associated with 3-year recurrence-free survival (RFS) rate of patients with gastric GISTs. RESULTS: Positive KCTD-12 expression was detected in 29 (74.4%) of the 39 gastric GIST tissues. The absence of KCTD-12 expression was associated with large tumor size, high mitotic count and NIH high-risk classification (P < 0.05). GISTs recurred after surgery in 10 of the 39 cases during the follow-up, and the 3-year RFS rate was 74.4%. Univariate analysis showed that tumor size, NIH risk classification, KCTD-12 expression and Ki-67 labeling index had an impact on the 3-year RFS rate of patients with GISTs of the stomach. On multivariate analysis, KCTD-12 [relative risk (RR) = 0.014; 95%CI: 0.001-0.320, P = 0.007] was shown to be a highly correlated predictor. The 3-year RFS rate of patients without KCTD-12 expression was only 30% compared with 89.7% in those with KCTD-12 expression (P = 0.001). The 3-year RFS rate of patients with KCTD-12 expression and a Ki-67 labeling index of 5% or less was 95.7%. The 3-year RFS rate of patients without KCTD-12 expression and a Ki-67 labeling index more than 5% was only 20%. CONCLUSION: KCTD-12 is a useful and reliable biomarker for the prognosis of GISTs of the stomach, especially when combined with Ki-67 labeling index. © 2015 Baishideng Publishing Group Inc. All rights reserved.

Hong M.,Nanjing Thoracic Hospital | Jiang Z.,Nanjing Southeast University | Zhou Y.-F.,Nanjing Jiangbei Peoples Hospital
Asian Pacific Journal of Cancer Prevention | Year: 2014

Background: To investigate the effects of double radiofrequency hyperthermia on Th1/Th2 cells in esophageal cancer patients treated with radiotherapy. Materials and Methods: 22 patients with esophageal cancer were divided into a radiotherapy group (10 cases) and a combined group (double radiofrequency hyperthermia combined with radiotherapy group, 12 cases). Both groups received conventional radiotherapy using a cobalt-60 therapy apparatus (TD60-66Gy/30-33F). Patients in the combined group also underwent double radiofrequency hyperthermia (2F/W, 8-10F). Before and after treatment, Th1, Th2, Tc1 and Tc2 cells in peripheral blood were determined with flow cytometry. Results: In the radiotherapy group, Th1 cell contents before and after radiotherapy were 17.5±5.26% and 9.69±4.86%, respectively, with a significant difference (p<0.01). The Th1/Th2 ratio was significantly decreased from 28.2±14.3 to 16.5±10.4 (p<0.01). In the combined group, Th1 cell content before radiotherapy was 15.9±8.18%, and it increased to 18.6±8.84 after radiotherapy (p>0.05), the Th1/Th2 ratio decreasing from 38.4±36.3 to 28.1±24.0 (p>0.05). Changes in Th2, Tc1 and Tc2 cell levels were not significant in the two groups before and after therapy (p>0.05). Conclusions: Double radiofrequency hyperthermia can promote the conversion from Th2 to Th1 cells, and regulate the balance of Th1/Th2 cells.

Ji W.,Nanjing University of Traditional Chinese Medicine | Chen Y.,Nanjing University of Traditional Chinese Medicine | Zhao X.,Nanjing University of Traditional Chinese Medicine | Guo Y.,Nanjing University of Traditional Chinese Medicine | And 4 more authors.
Molecular Medicine Reports | Year: 2015

The aim of the current study was to explore the effects and possible mechanisms of tripterygium glycosides tablet (TGT) in the treatment of active ankylosing spondylitis (AS). Thirty-six patients with active AS were given a 20 mg TGT treatment three times per day for 12 weeks, and 21 unrelated healthy controls were recruited as the control group. Efficacy measures included the Bath AS disease activity index (BASDAI), erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) prior and subsequent to TGT treatment. Serum dickkopf homolog 1 (DKK1) and interleukin-17 (IL-17) levels before and after TGT treatment were assessed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and ELISA assay. The levels of several serum biomarkers were determined by ELISA, including receptor activator of nuclear factor κ-B ligand (RANKL), osteoprotegerin (OPG), bone alkaline phosphatase (BAP), bone morphogenetic protein-2 (BMP-2), matrix metalloproteinase-3 (MMP-3), cross-linked telopeptide of type II collagen (CTX-II), vascular endothelial growth factor (VEGF), and prostaglandin E2 (PGE2). After 12 weeks of TGT treatment, the BASDAI score of the patients was significantly reduced (P<0.05), their levels of ESR and CRP were significantly reduced to a normal level (P<0.05, P<0.05), RT-PCR and ELISA showed a significant increase in the level of DKK1 expression (P<0.05) and a significant decreased IL-17 expression (P<0.05), there was a significant increase in the expression of OPG, BAP and BMP-2 (P<0.01, P<0.01, P<0.01) and a significant reduction in the expression levels of RANKL, CTX-II. MMP-3, PGE2, and VEGF (P<0.01, P<0.01, P<0.01, P<0.05, P<0.01) compared with those of the controls. TGT is effective at improving the signs and symptoms of patients with AS through the regulation of serum biomarkers, and the mechanisms may be associated with the anti-inflammatory effect, inhibition of new bone formation and potential bone-protective effects.

Feng H.,Nanjing Jiangbei Peoples Hospital | Yin J.,Nanjing Jiangbei Peoples Hospital | Han Y.-P.,Nanjing Medical University | Zhou X.-Y.,Nanjing Medical University | Zhang G.-X.,Nanjing Medical University
International Journal of Clinical and Experimental Medicine | Year: 2015

CD64 was up-regulated in infection diseases, but there was no report about the change of CD64 in chronic hepatitis B virus (HBV) infection. The purpose of this study was to determine whether there was a dynamic change of CD 64 index and to judge the value to antiviral treatment. 96 CHB patients were enrolled and selected 33 healthy adults as control. We detected the level of CD64, found the level of CD64 were significantly increased in chronic HBV infection patients, especially the lymphocyte CD64 (8.12 ± 0.23 vs. 6.25 ± 0.27; P < 0.001). Further, we proved CD64 index was increased in various stages of chronic HBV infection. ROC curve analysis showed the level of lymphocyte CD64 had higher AUC value than neutrophil or monocyte. Then we monitor longitudinally the impact of the treatment with interferon-α and found that the suppression of viral replication induced by interferon-α resulted in a decrease in CD64 index. In conclusion, this study showed that CD64 index was increased in chronic HBV infection patients and changed with the course of disease, the therapy of interferon-α would correct it, and analysis prompted that the level of lymphocyte CD64 would be more suitable for as a biomarker to judge the condition of chronic HBV infection and the curative effect of interferon-α treatment. © 2015, E-Century Publishing Corporation. All rights reserved.

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