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Liu X.,Nanjing Agricultural University | Liu X.,Nanjing Institute of Physical Education | Pan S.,Nanjing Agricultural University | Li X.,Nanjing Agricultural University | And 3 more authors.
European Journal of Nutrition | Year: 2015

Purpose: We tested the hypothesis that maternal low-protein (LP) diet during gestation and lactation can program myostatin (MSTN) signaling and protein synthesis in skeletal muscle of offspring at weaning stage (35 days). Methods: Fourteen Meishan sows were fed either LP or standard-protein diets throughout gestation and lactation, male offspring piglets were killed at weaning stage and longissimus dorsi (LD) muscles were taken. The cross-sectional areas (CSA) of LD muscles were measured by hematoxylin and eosin staining. The levels of free amino acids in plasma were measured by amino acid auto-analyzer. Proteins and mRNA were determined by Western blot and RT-qPCR, respectively. Results: Body weight, LD muscle weight and the myofiber CSA were significantly decreased (P < 0.05) in LP piglets; meanwhile, the concentration of branched-chain amino acids was also significantly decreased (P < 0.001). MSTN protein content tended to be higher (P = 0.098) in LP piglets, while the expression of MSTN receptors, activin type II receptor-beta and transforming growth factor type-beta type I receptor kinase, was significantly up-regulated (P < 0.05). Furthermore, p38 mitogen-activated protein kinase, the downstream signaling factor of MSTN, was also enhanced significantly (P < 0.05). In addition, key factors of translation initiation, phosphorylated eukaryotic initiation factor 4E and the 70 kDa ribosomal protein S6 kinase, were significantly decreased (P < 0.05) in LP piglets. Conclusions: Our results suggest that maternal LP diet during gestation and lactation affects MSTN signaling and protein synthesis in skeletal muscle of offspring at weaning stage. © 2014, Springer-Verlag Berlin Heidelberg.

Qiu Y.,Shandong University | Zhu Y.,Tangshan Branch of Jinling General Hospital | Jia W.,Shanghai University | Chen S.,Southern Medical University | Meng Q.,Nanjing Institute of Physical Education
Complementary Therapies in Medicine | Year: 2014

Objective: To investigate the effect of spa adjuvant therapy on diabetic lower extremity arterial disease (LEAD). Methods: 128 patients with type II diabetes were separated into three groups according to the degree of lower extremity vascular stenosis. Patients within each group were then randomly divided to receive no treatment (control) or spa adjuvant therapy (treatment). Clinical symptoms, blood pressure and hemodynamic analyses were compared between control and treatment groups by Chi square or t-test. Results: After adjuvant therapy with spa, patients' pain, numbness, and cold sensation were significantly improved compared with control groups (P<. 0.05). Spa adjuvant therapy also significantly increased the dorsalis pedis pulse and systolic peak velocity ratio of patients with mild lower extremity vascular stenosis compared with control groups (P<. 0.05), while there were no significant differences between the two groups for patients with moderate and severe stenosis (P>. 0.05). Both in the spa and control groups, there were no significant differences before and after medication for fasting, 2-h postprandial blood glucose and glycosylated hemoglobin (HbA1C) analyses (P>. 0.05). Conclusions: Spa adjuvant therapy can significantly alleviate lower extremity pain, numbness, and cold sensory symptoms in diabetic LEAD patients with stenosis. Moreover, in LEAD patients with mild stenosis, spa adjuvant therapy also improves the dorsalis pedis pulse and systolic peak velocity ratio, suggesting a potential role for spa therapy as an early intervention strategy to treat the initial stages of disease. © 2014 Elsevier Ltd.

Ya-Wei S.,Nanjing Institute of Physical Education | Jing-Guang Q.,Nanjing Institute of Physical Education
Proceedings of the 2011 International Conference on Future Computer Science and Education, ICFCSE 2011 | Year: 2011

Using the German Zebris measuring systems for plantar force distribution to measure the youth trampolinists' static balance stability who attended the National Youth Trampoline Championship, Guilin, Guangxi from the 18th - 23th of November 2009. We got 6 balance parameters results which are ellipse's width, height, area, track length, horizontal offset and vertical offset. Using these 6 parameters for comparing the differences of balance stability when youth trampolinists eyes open stand on both feet with closed and eyes open stand on single foot with closed. Through this way to discuss the features of trampolinists' static balance performance and provide a theoretical basis for trampolinists' scientific athlete selection and training. © 2011 IEEE.

Qian J.-G.,Nanjing Institute of Physical Education | Su Y.,Nanjing Institute of Physical Education | Song Y.-W.,Nanjing Institute of Physical Education | Qiang Y.,Nanjing Institute of Physical Education | Zhang S.,University of Tennessee at Knoxville
Journal of Human Kinetics | Year: 2012

The purpose of this study was to establish a multi-segment dynamic model in the LifeMOD to examine kinematics of the center of mass and foot, and muscle forces of selected upper extremity muslces during a double-leg circle (DLC) movement on pommel horse in gymnastics and compared with three-dimensional kinematics of the movement and surface electromyographic (sEMG) activity of the muscles. The DLC movement of one elite male gymnast was collected. The three-dimensional (3D) data was imported in the Lifemod to create a full-body human model. A 16-Channel surface electromyography system was used to collect sEMG signals of middle deltoid, biceps brachii, triceps brachii, latissimusdorsi, and pectoralis major. The 3D center of mass and foot displacement showed a good match with the computer simulated results. The muscle force estimations from the model during the four DLC phases were also generally supported by the integrated sEMG results, suggesting that the model was valid. A potential application of this model is to help identify shortcomings of athletes and help establish appropriate training plans errors in the DLC technique during training. © Editorial Committee of Journal of Human Kinetics.

Song Y.-W.,Nanjing Institute of Physical Education | Sun W.,Nanjing Institute of Physical Education | Kou H.-J.,Nanjing Institute of Physical Education
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010

BACKGROUND: Kinematics studies the movement of the objects using a method of geometry, with strength and quality not taken into consideration; Kinetics major studies the force of people acting on objects and its relationship with the movement of objects. OBJECTIVE: Through an introduction to kinematics and kinetics research equipment, this study was aimed to lead to the current status of kinematics and kinetics, to provide for experimental basis for the research of new equipment and gait experiments. METHODS: Taking "gait, kinetics, kinematics" in English for the search terms, Pubmed database (1999-01/2009-06) was retrieved. Taking "gait, kinematics, kinetics" in Chinese for the search terms, CNKI database (1999-01/2009-06) was searched. Literatures were limited to English and Chinese languages. The kinematics and kinetics research equipment regarding the gait were included, while other kinematics and kinetics experiments were excluded, also outdated and repetitive researches were excluded. RESULTS AND CONCLUSION: Totally 2 367 literature were obtained from the computer screen, and 33 documents of them were involved for analysis according to the inclusion and exclusion criteria. In case of gait analysis method, at home and abroad scholars have carried out extensive studies although the content are quite different, they used identical instruments and methods in the testing of gait, in spite of photography or video recording were applied to calculate and measure kinematic parameters, the force platform was all used to record kinetic parameters. Also there are a small number of studies using the distribution of plantar pressure to measure more precise foot-ground pressure distribution, and then proceed to a comprehensive statistical analysis. Kinetics and kinematics gait analysis is applied not only in rehabilitation medicine, but also widely in athletics sports and the related footwear manufactures, the commonly used method is the plane fixed-point photograph and force platform analysis.

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