Nanjing Institute of Environmental Sciences

Nanjing, China

Nanjing Institute of Environmental Sciences

Nanjing, China
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Patent
Nanjing Institute of Environmental Sciences, Chongqing University of Arts and Sciences | Date: 2016-10-21

A detection device for simultaneous in-situ measurement of dissolved oxygen at different submerged plant leaf-water interface levels. The detection device includes a dissolved oxygen micro-optrode host. A plurality of detection probes are externally connected to the dissolved oxygen micro-optrode host and can extend out probes. The detection device includes a leaf clamp having an upper clamping head, a lower clamping head, a hinged shaft and a clamping handle. Each of the upper and lower clamping head includes a plurality of water passing cavities penetrating through the back and the front thereof. Each of the upper and lower clamping head includes a plurality of slots horizontally extending inwardly of the corresponding clamping head. The detection device includes a plurality of insertion pieces having a probe groove. The detection probe is locatable in the respective probe groove for fixation. The insertion pieces are insertable and fixable in the slots.


Patent
Nanjing Institute of Environmental Sciences, Chongqing University of Arts and Sciences | Date: 2016-10-21

A spliced detection apparatus for simultaneous wide-range in-situ detection of dissolved oxygen in a sludge-water interface of the present invention comprises a dissolved oxygen shimmer host, several detection probes, a casing and detection kits, several vertically through seat chambers being disposed in the casing, the detection kits can be placed and fixed in the seat chambers, the detection kits may be placed on the sludge-water interface, and each detection kit is provided with a vertically through water passage chamber, while the front side face and the back side face of the detection kit are respectively provided with several front slots and rear slots horizontally extending into the detection kit respectively, the detection apparatus further comprises tabs, with probe grooves being provided on the tabs, each detection probe can be put into the probe groove of the corresponding tab and fixed therein, the tabs may be inserted either into the front slots or the rear slots and can enable the probe tips of the detection probes to be placed in the water passage chamber, the detection apparatus can carry out simultaneous wide-range detection of the dissolved oxygen at different heights and horizontal positions of the sludge-water interface, and thus is quick and convenient for use.


Ji G.,Nanjing Institute of Environmental Sciences | Yan L.,Nanjing Medical University | Liu W.,Nanjing Medical University | Qu J.,Nanjing Medical University | Gu A.,Nanjing Medical University
Toxicology Letters | Year: 2013

8-Oxoguanine DNA glycosylase 1 (OGG1) plays an important role in repairing oxidative DNA damage induced by chemical agents, such as tobacco. This study examined the effects of OGG1 Ser326Cys polymorphism and cigarette smoking, alone or combined, on sperm oxidative DNA damage and the risk of male infertility. A total of 620 idiopathic infertile subjects and 480 fertile controls were recruited in this study. Sperm 8-hydroxydeoxyguanine (8-OHdG) was measured by immunofluorescent assay using flow cytometry and genotypes were determined by OpenArray platform with a chip-based Taq-Man genotyping technology. Our results demonstrated that both cigarette smoking and OGG1 polymorphism can affect the sperm 8-OHdG levels. Individuals with variant Cys/Cys homozygote showed higher levels of sperm 8-OHdG than wide-type homozygote carriers (Ser/Ser). Stratified analysis found that the association between OGG1 polymorphism and sperm 8-OHdG levels was only observed among smokers with pack-years ≥5 but not among those subjects with pack-years<5 (pack-years=packs smoked per day×years as a smoker). Further analysis based on the case-control study revealed that variant allele (Cys) of OGG1 was significantly associated with male infertility risk in a dominant model (OR=1.35, 95% CI: 1.01-1.82; trend P<0.001). Furthermore, we found a significant gene-environment interaction between OGG1 Ser326Cys polymorphism and cigarette smoking in relation to male infertility risk (Pinteration=0.0003). These findings provided the first evidence about potential interactive effects of OGG1 polymorphism and cigarette smoking on male infertility risk. © 2013 Elsevier Ireland Ltd.


Liu J.-J.,Nanjing University | Zhao M.-Y.,Nanjing University | Zhang X.,Nanjing University | Zhao X.,Nanjing Institute of Environmental Sciences | Zhu H.-L.,Nanjing University
Mini-Reviews in Medicinal Chemistry | Year: 2013

Within the past years, many researches on the synthesis, structure-activity relationships (SAR), antitumor, antiinflammatory and anti-bacterial activities of the pyrazole derivatives have been reported. Several pyrazole derivatives possess important pharmacological activities and they have been proved useful materials in drug research. Pyrazole derivatives play an important role in antitumor agents because of their good inhibitory activity against BRAFV600E, EGFR, telomerase, ROS Receptor Tyrosine Kinase and Aurora-A kinase. In addition, pyrazole derivatives also show good antiinflammatory and anti-bacterial activities. In this review, the bioactivities of the pyrazole derivatives mentioned above will be summarized in detail. We sincerely hope that increasing knowledge of the SAR and cellular processes underlying the bioactivity of pyrazole derivatives will be beneficial to the rational design of new generation of small molecule drugs. © 2013 Bentham Science Publishers.


Luo Y.,Jinling Institute of Technology | Xie W.,Nanjing Institute of Environmental Sciences
Food Chemistry | Year: 2012

The effect of dephytinisation, using an exogenous phytase under optimal conditions (pH 5.5, 37 °C), and subsequent removal of the soaking solution after processing, on the bioavailability of iron from faba bean (Vicia faba L.) flour was studied. Soaking of the faba bean flour led to a considerable reduction in the content of iron (39%), whereas a lower reduction in iron content (10%), was obtained after additional treatment with phytase, than in the soaked faba bean flour. The digestive utilisation of iron from the raw and soaked faba bean flours by growing rats was negligible, but increased significantly as a result of phytase treatment. The low iron absorption obtained for the former two treatments, during an experimental period of 10 days, was not reflected in any of the haematological indices (red blood count, haemoglobin, haematocrit) or tissues (femur, heart, kidney) studied, with the exception of the sternum. The latter appears to be a useful indicator of iron bioavailability. © 2012 Elsevier Ltd. All rights reserved.


Luo Y.-W.,Jinling Institute of Technology | Xie W.-H.,Nanjing Institute of Environmental Sciences
CYTA - Journal of Food | Year: 2013

Effects of several physical treatments (soaking, dehulling, ordinary cooking, microwave cooking, and autoclaving) on the level of antinutrtional factors and in vitro protein digestibility (IVPD) of green and white faba beans were investigated. The raw green and white faba bean seeds were found to contain phytic acid (PA), tannins, trypsin inhibitor activity (TIA), and lectin activity, but aamylase inhibitor was absent. Dehulling significantly increased the levels of PA, TIA, decreased the level of tannins, but lectin activity was not changed. Cooking methods differently affected the levels of the antinutrtional factors. Thus, PA increased but tannins were not changed; on the other hand, TI and lectin activities were inactivated; ordinary cooking and autoclaving were the most effective in both seeds. For combination effect, soaking following cooking treatments significantly decreased PA, tannins, and lectin activity but increased TIA in both seeds. Moreover, dehulling following soaking and cooking methods resulted in a significant increase of PA and TIA. In vitro protein digestibility of raw green and white faba bean seeds were 72.65 and 73.28%, respectively, and it was improved by all processing methods; soaking-dehulling after autoclaving was the most effective in both seeds. Although some treatments increased the level of antinutrtional factors, they improved IVPD. Therefore, the studied antinutrients are not the only responsible factor for lowering IVPD. © 2012 Taylor & Francis.


Li X.,Nanjing Institute of Environmental Sciences | Li X.,CAS Nanjing Institute of Soil Science | Liu B.,Nanjing Institute of Environmental Sciences
PLoS ONE | Year: 2013

Transgenic insect-resistant cotton has been released into the environment for more than a decade in China to effectively control the cotton bollworm (Helicoverpa armigera) and other Lepidoptera. Because of concerns about undesirable ecological side-effects of transgenic crops, it is important to monitor the potential environmental impact of transgenic insect-resistant cotton after commercial release. Our 2-year study included 1 cotton field where non-transgenic cotton had been planted continuously and 2 other cotton fields where transgenic insect-resistant cotton had been planted for different lengths of time since 1997 and since 2002. In 2 consecutive years (2009 and 2010), we took soil samples from 3 cotton fields at 4 different growth stages (seedling, budding, boll-forming and boll-opening stages), collected soil nematodes from soil with the sugar flotation and centrifugation method and identified the soil nematodes to the genus level. The generic composition, individual densities and diversity indices of the soil nematodes did not differ significantly between the 2 transgenic cotton fields and the non-transgenic cotton field, but significant seasonal variation was found in the individual densities of the principal trophic groups and in the diversity indices of the nematodes in all 3 cotton fields. The study used a comparative perspective to monitor the impact of transgenic insect-resistant cotton grown in typical 'real world' conditions. The results of the study suggested that more than 10 years of cultivation of transgenic insect-resistant cotton had no significant effects-adverse or otherwise-on soil nematodes. This study provides a theoretical basis for ongoing environmental impact monitoring of transgenic plants. © 2013 Li, Liu.


Zhou H.,Nanjing Institute of Environmental Sciences | Gao C.,Nanjing University
Environmental Management | Year: 2011

Agricultural nonpoint phosphorus (P) pollution is a primary cause of eutrophication in many freshwater systems. Identifying areas that are at high risk for P loss in a watershed and concentrating management efforts on these smaller sections is a more effective method for limiting P loss than implementing general strategies over a broad area. A modified P index scheme was used to assess the risk of P loss and identify critical source areas in the Chaohu Lake watershed on a regional scale. In the new P ranking scheme, soil P sorption index (PSI) and degree of P saturation (DPS) were introduced as source factors to represent the inherent ability of P transport in the soil-water interface. Distance from P sources to Chaohu Lake was also considered as a transport factor to take into account P degradation from source to the final receiving water. The ranking scheme was modified to use available data on the regional scale. P index calculation results showed high spatial variation of P loss risk in the Chaohu Lake watershed. The highest risk areas focused on the downstream parts of the main rivers that discharge into Chaohu Lake. The induction of new components into the P index calculation makes it possible to identify critical source areas of nonpoint P loss on a regional scale, thus allowing decision makers to implement best management practices (BMPs) in such a manner as to minimize P loss to sensitive watercourses. © 2011 Springer Science+Business Media, LLC.


Cao W.,Xuzhou Institute of Technology | Zhang H.,Nanjing Institute of Environmental Sciences | Wang Y.,Xuzhou Institute of Technology | Pan J.,CAS Nanjing Institute of Geography and Limnology
Ecological Engineering | Year: 2012

Polluted surface water was remediated in a bioreactor using biofilms on filamentous bamboo in batch and continuous flow modes. The COD cr (Chemical Oxygen Demand, using K 2Cr 2O 7 as oxidizer) removal rate of the enhanced systems increased more than 13% relative to a controlled system. In the batch reactor with 4-h cycles, the COD cr was mainly removed by the biofilm on the filamentous bamboo. The removal rate of the COD Mn (permanganate index), NH 4 +-N (ammonia nitrogen), turbidity, and total bacteria were 11.2-74.3%, 2.2-56.1%, 20-100%, and more than 88.6% in a continuous flow biofilm reactor with a 3.5h retention time. The biofilms contained diverse organisms including protozoa and metazoa. © 2012 Elsevier B.V.


Ji Y.,Nanjing Agricultural University | Dong C.,Nanjing Agricultural University | Kong D.,Nanjing Institute of Environmental Sciences | Lu J.,Nanjing Agricultural University
Journal of Hazardous Materials | Year: 2015

The widespread occurrence of atrazine in waters poses potential risk to ecosystem and human health. In this study, we investigated the underlying mechanisms and transformation pathways of atrazine degradation by cobalt catalyzed peroxymonosulfate (Co(II)/PMS). Co(II)/PMS was found to be more efficient for ATZ elimination in aqueous solution than Fe(II)/PMS process. ATZ oxidation by Co(II)/PMS followed pseudo-first-order kinetics, and the reaction rate constant (kobs) increased appreciably with increasing Co(II) concentration. Increasing initial PMS concentration favored the decomposition of ATZ, however, no linear relationship between kobs and PMS concentration was observed. Higher efficiency of ATZ oxidation was observed around neutral pH, implying the possibility of applying Co(II)/PMS process under environmental realistic conditions. Natural organic matter (NOM), chloride (Cl-) and bicarbonate (HCO3 -) showed detrimental effects on ATZ degradation, particularly at higher concentrations. Eleven products were identified by applying solid phase extraction-liquid chromatography-mass spectrometry (SPE-LC/MS) techniques. Major transformation pathways of ATZ included dealkylation, dechlorination-hydroxylation, and alkyl chain oxidation. Detailed mechanisms responsible for these transformation pathways were discussed. Our results reveal that Co(II)/PMS process might be an efficient technique for remediation of groundwater contaminated by ATZ and structurally related s-triazine herbicides. © 2014 Elsevier B.V.

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