Nanjing Institute for the Comprehensive Utilization of Wild Plants

Nanjing, China

Nanjing Institute for the Comprehensive Utilization of Wild Plants

Nanjing, China
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Long L.,Nanjing Forestry University | Lin Q.,Nanjing Institute for the Comprehensive Utilization of Wild Plants | Yao Q.,South China Agricultural University | Zhu H.,Guangdong Institute of Microbiology
3 Biotech | Year: 2017

This study was aimed to investigate the diversity and function of bacterial population associated with Gigaspora margarita spores. The fungus was propagated in sterilized sand/soil pots using alfalfa (Medicago sativa), grain sorghum (Sorghum bicolor), or maize (Zea mays) as host plants, or in sterilized vermiculite pots using alfalfa as host plants, respectively. Bacteria were isolated from the new-formed spores using diluted plate method, and typical bacterial isolates were identified according to 16S rRNA gene phylogenetic analysis. Total 43 bacterial isolates affiliated to three phyla and 23 genera were obtained. The spore-associated bacterial communities were obviously different among the four source spores, suggesting that plant species or substrates could influence the bacterial population. Bacillus and Streptomyces were most frequently associated with the fungal spores. Function analysis of these bacteria by plate tests, it was found that about 30.2% isolates stimulated the spore germination, five out of seven tested isolates improved the hyphal growth, total 57.5% of the tested isolates solubilized phosphorus at different levels, 15% isolates degraded chitin, and a few isolates suppressed the growth of Escherichia coli or Staphylococcus aureus. In pot experiment, three bacterial isolates (belonging to Curtobacterium, Ensifer, or Bacillus, respectively) displayed improvement effect on alfalfa growth and/or the colonization of roots by G. margarita. © 2017, The Author(s).


Yan T.-L.,Nanjing Institute for the Comprehensive Utilization of Wild Plants | Yan T.-L.,Nanjing University | Yan T.-L.,Nature Technology | Bai L.-F.,Jiangsu University | And 9 more authors.
ChemMedChem | Year: 2017

Vascular endothelial growth factor receptor 2 (VEGFR2) has been proven to play a major role in the regulation of tumor angiogenesis. A series of novel glycyrrhetic acid derivatives were synthesized and evaluated for their VEGFR2 inhibitory activity as well as their antiproliferative properties against four cancer cell lines (MCF-7, HeLa, HepG2, and A549). In vitro biological evaluations against these human tumor cell lines indicate that most of the prepared compounds have antiproliferative activities; compound 3 a (3β-hydroxy-30-(4-phenyl-1-piperazinyl)olean-12-ene-11,30-dione) exhibited the best inhibitory activity against MCF-7 cells, with an IC50 value of 1.08 μm. Compound 3 a also showed the most potent inhibitory activity against VEGFR2 tyrosine kinase, with an IC50 value of 0.35 μm. Docking simulations were performed with the aim of discovering the binding mode of compound 3 a, and the results indicate that 3 a could bind at the VEGFR2 active site. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim


Peng X.,Nanjing Normal University | Peng X.,Shanxi Datong University | Cao Y.,Nanjing Normal University | Yang Z.,Nanjing Normal University | And 4 more authors.
Pakistan Journal of Botany | Year: 2012

Medicinal plants Apocynum venetum L. and Poacynum pictum (Schrenk) Baill. having similar efficacy belong to two genera. However, many similarities of morphological characteristics in pollen, seeds, leaves and stems etc. between the two species recommend it under one genus Apocynum L. To address this question, we selected DNA sequences [nuclear rDNA internal transcribed spacer (ITS), trnL intron and trnL-trnF intergenic spacer (IGS) sequence] and RAPD markers to assess the relationship between them, with samples from 10 populations of A. venetum and 5 populations of P. pictum from China. ITS sequences from all samples of A. venetum and P. pictum were same, and only two different sites were found in the trnL intron region and one different site in the trnL-trnF IGS region. The genetic distance between A. venetum and P. pictum calculated upon these regions was much smaller than both the intergeneric and interspecies genetic distances calculated upon the same regions in the vast majority of plants. This reaffirmed the notion that A. venetum and P. pictum had a close genetic relationship. Results from RAPD markers showed that there was high similarity and still stable difference between A. venetum and P. pictum. The genetic difference between A. venetum and P. pictum was greater than the differences among populations within species. Our studies recommend that A. venetum and P. pictum were genetic close-related species and they may be treated within one genus.


Long L.,Nanjing Forestry University | Ding D.,Nanjing Forestry University | Han Z.,Nanjing Forestry University | Zhao H.,Nanjing Forestry University | And 2 more authors.
Journal of Applied Microbiology | Year: 2016

Aims: To characterize the hemicellulolytic and cellulolytic enzymes from novel fungi, and evaluate the potential of novel enzyme system in releasing ferulic acid (FA) from biomass resource. Methods and Results: A hemicellulolytic and cellulolytic enzyme-producing fungus 4-14 was isolated from soil by Congo red staining method, and identified as Eupenicillium parvum based on the morphologic and molecular phylogenetic analysis. The optimum temperature of fungal growth was 37°C. Hemicellulolytic and cellulolytic enzymes were produced by this fungus in solid-state fermentation (SSF), and their maximum activities were 554, 385, 218, 2·62 and 5·25 U g−1 for CMCase, xylanase, β-glucosidase, FPase and FAE respectively. These enzymes displayed the best catalytic ability at low pH values (pH 4·5–5·0). The optimum temperatures were 70°C, 70°C, 75°C and 55°C for CMCase, β-glucosidase, xylanase and FAE respectively. CMCase, xylanase and FAE were stable at different pHs or high temperature (60°C). Enzymatic hydrolysis experiment indicated that the maximum (76·8 ± 4)% of total alkali-extractable FA was released from de-starched wheat bran by the fungal enzyme system. Conclusions: High activities of thermotolerant CMCase, β-glucosidase, xylanase and FAE were produced by the newly isolated fungus E. parvum 4-14 in SSF. The fungal enzyme system displayed high efficiency at releasing FA from wheat bran. Significance and Impact of the Study: This study provides a new fungal strain for researches of novel hemicellulolytic and cellulolytic enzymes and will improve the bioconversion and utilization of agricultural by-products. © 2016 The Society for Applied Microbiology


PubMed | Nanjing Institute for the Comprehensive Utilization of Wild Plants and Nanjing Forestry University
Type: Journal Article | Journal: Journal of applied microbiology | Year: 2016

To characterize the hemicellulolytic and cellulolytic enzymes from novel fungi, and evaluate the potential of novel enzyme system in releasing ferulic acid (FA) from biomass resource.A hemicellulolytic and cellulolytic enzyme-producing fungus 4-14 was isolated from soil by Congo red staining method, and identified as Eupenicillium parvum based on the morphologic and molecular phylogenetic analysis. The optimum temperature of fungal growth was 37C. Hemicellulolytic and cellulolytic enzymes were produced by this fungus in solid-state fermentation (SSF), and their maximum activities were 554, 385, 218, 262 and 525Ug(-1) for CMCase, xylanase, -glucosidase, FPase and FAE respectively. These enzymes displayed the best catalytic ability at low pH values (pH 45-50). The optimum temperatures were 70C, 70C, 75C and 55C for CMCase, -glucosidase, xylanase and FAE respectively. CMCase, xylanase and FAE were stable at different pHs or high temperature (60C). Enzymatic hydrolysis experiment indicated that the maximum (7684)% of total alkali-extractable FA was released from de-starched wheat bran by the fungal enzyme system.High activities of thermotolerant CMCase, -glucosidase, xylanase and FAE were produced by the newly isolated fungus E.parvum 4-14 in SSF. The fungal enzyme system displayed high efficiency at releasing FA from wheat bran.This study provides a new fungal strain for researches of novel hemicellulolytic and cellulolytic enzymes and will improve the bioconversion and utilization of agricultural by-products.


Yang J.,Nanjing Normal University | Cao Y.,Nanjing Normal University | Yang Z.,Nanjing Normal University | Zhang W.,Nanjing Institute for the Comprehensive Utilization of Wild plants | And 2 more authors.
Acta Agriculturae Scandinavica Section B: Soil and Plant Science | Year: 2013

Saline lands are characterized by salinity and nutrient deficiency and there is an ever increasing need for economical, adaptable plant species to rejuvenate these lands. In this study, we determined the suitability and tolerance of Euphorbia lathyris L. (Caper spurge), a well-known biofuel plant, as a sustainable candidate to colonize saline lands. We investigated the germination rate, seedling growth, solute change and anti-oxidative enzyme activities etc. under salt stress conditions. Our results showed that Caper spurge seeds prefer to germinate under nonsaline environments and high salt stress induced temporary dormancy during germination, but did not completely hamper the viability of the seeds. The seedling biomass increased without any visible distress symptoms in the presence of NaCl not over 171 mM. Further increase in NaCl concentration had a negative impact on the seedling growth. These demonstrate that Caper spurge seedlings have the potential to grow in saline lands. The salinity tolerance of Caper spurge seedlings was closely associated with the regional distribution of Na+ in roots, stable absorption of Ca2 +, K+ and Mg2 +, accumulation of organic solutes, and increased activity of superoxide dismutase (SOD) and catalase (CAT) enzymes. However, excessive accumulation of Na+, sharp increase of superoxide (O2 -), H2O2, malonaldehyde (MDA) and cell membrane leakage, reduction of osmoprotectants, and decreased activities of CAT and ascorbate peroxidase (APX) etc. under high salinity might be the reasons for the restrained seedling growth. © 2013 Copyright Taylor and Francis Group, LLC.

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