Nanjing General Hospital of Nanjing Military Command
Nanjing General Hospital of Nanjing Military Command
Liu Y.-W.,Xuzhou Medical College |
Liu Y.-W.,Nanjing General Hospital of Nanjing Military Command |
Zhu X.,Xuzhou Medical College |
Yang Q.-Q.,Xuzhou Medical College |
And 7 more authors.
Psychopharmacology | Year: 2013
Rationale: Evidences indicate that methylglyoxal, a highly reactive metabolite of hyperglycemia, can enhance protein glycation, oxidative stress, or inflammation. Mangiferin, a polyphenol compound of C-glucoside, has many beneficial biological activities, including anti-inflammation, anti-oxidation, neuroprotection, cognitive enhancement, etc. Whether mangiferin alleviates diabetes-associated cognitive impairment is still unclear. Objectives: The present study was designed to investigate the effects of mangiferin on the behavioral deficits of diabetic rats induced by streptozotocin; the mechanisms associated with methylglyoxal toxicity are especially investigated. Methods: Diabetic rats were treated with mangiferin (15, 30, and 60 mg/kg, p.o.) for 9 weeks. Cognitive performances were evaluated with the Morris water maze. Hippocampus and blood were obtained for evaluation of the effects of mangiferin on protein glycation, oxidative stress, and inflammation in diabetic state. Results: Mangiferin significantly improved the behavioral performances of diabetic rats, evidenced by a decrease in escape latency as well as increases in numbers of crossing the platform and percentage of time spent in the target quadrant, which were accompanied by decreases in the levels of advanced glycation end-products and their receptor (RAGE), interleukin-1β, TNF-α, and malondialdehyde and increases in the activity and expression of glyoxalase 1 as well as glutathione level in the hippocampus of diabetic rats. Furthermore, mangiferin produced a significant decrease in malondialdehyde level and increased glutathione level and superoxide dismutase activity in the serum of diabetic rats. Conclusions: This study demonstrates that mangiferin can markedly ameliorate diabetes-associated cognitive decline in rats, which is done likely through suppressing methylglyoxal hyperactivity (promoting protein glycation, oxidative stress, and inflammation) mediated noxious effects. © 2013 Springer-Verlag Berlin Heidelberg.
Lu X.-X.,Nanjing University |
Chen Y.-T.,Nanjing University |
Feng B.,Nanjing University |
Mao X.-B.,Nanjing University |
And 2 more authors.
World Journal of Gastroenterology | Year: 2013
Aim: To investigate the expression of CD73 and hypoxia- inducible factor-1α (HIF-1α) in human gastric carcinoma, and explore their clinical significance and prognostic value. Methods: CD73 and HIF-1α expressions were detected by immunohistochemistry in consecutive sections of tissue samples from 68 gastric carcinoma patients. The peritumor tissues 2 cm away from the tumor were obtained and served as controls. The presence of CD73 and HIF-1α was analyzed by immunohistochemistry using the Envision technique. Results: CD73 and HIF-1α expressions in gastric carcinoma were significantly higher than those in gastric mucosal tissues as control (P < 0.001) and showed a close correlation (Spearman r = 0.390, P = 0.001). Overexpression of CD73 was positively correlated with differentiation of tumor (P = 0.000), histopathology (P = 0.041), depth of invasion (P < 0.001), nodal status (P = 0.003), metastasis (P = 0.013), and the American Joint Committee on Cancer (AJCC) stage (P < 0.001). High expression of HIF-1α was positively correlated with tumor diameter (P = 0.031), depth of invasion (P = 0.022), and AJCC stage (P = 0.035). The overall survival rate was low in the patients with high expression of CD73 (P < 0.001). Moreover, CD73+/HIF-1α+ patients had the worst prognosis (P < 0.001). CD73 expression was proven to be an independent predictor for patients with gastric carcinoma by both multivariate Cox regression analysis (P = 0.021) and receiver operating characteristic curves (P = 0.001). 5: CD73 expression correlates closely with HIF-1α expression in gastric carcinoma. CD73 could be an independent prognostic indicator for gastric carcinoma. © 2013 Baishideng. All rights reserved.
Jiang M.,Nanjing General Hospital of Nanjing Military Command
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2013
The aim of this study was to explore the changes of the extracellular matrix in nasal mucosa by a guinea pig model of prolonged allergic-induced rhinitis. Thirty-two male Hartley guinea pigs were randomly divided into four groups: allergen challenged groups (Group 2 w, Group 6 w and Group 12 w) and a control group. Ovalbumin-sensitized guinea pigs were repeatedly challenged with allergen twice a week from 2 weeks to 12 weeks. Matched control groups were challenged with physiological saline. Nasal mucosa were obtained from the animals killed. Hematoxylin-Eosin, Masson's trichrome, and immunohistochemical staining against transforming growth factor-beta1 (TGF-beta1), Collagen III and Collagen I were performed to nasal mucosa. (1) Pathological examination showed obvious infiltration of eosinophils and the enlarged thickness of epithelial layer of nasal mucosa in the experiment groups. (2) The area ratios of blue stained in the extracellular matrix of nasal mucosa were increased. The area ratios of blue stained were statistically different in Group 6 w and Group 12 w compared with the control group. (3) The increasing absorbance of TGF-beta1 were statistically different in the experiment groups with the control group. The absorbance of Collagen III and Collagen I showed a rising trend along prolonged allergen challenged in the experiment groups. Prolonged allergen challenge and the inflammation of nasal mucosa, can lead to the increasing of the inflammation relevant factors and the deposit of collagen in the extracellular matrix of nasal mucosa.
Cai H.,Nanjing General Hospital of Nanjing Military Command
Zhen ci yan jiu = Acupuncture research / [Zhongguo yi xue ke xue yuan Yi xue qing bao yan jiu suo bian ji] | Year: 2011
To study the relationship between serum leptin and insulin resistance, and to analyze the effect of acupuncture on serum leptin level in patients with type-II diabetes mellitus (DM). A total of 80 type-II DM patients were randomized into acupuncture and medication groups. Acupuncture was applied to Yishu (EX), Feishu (BL13), Pishu (BL 20), etc. according to syndrome identification. The treatment was given once every other day for 12 weeks. For patients in the medication group, Glibenclamide (2.5-7.5 mg/time, 1-2 times/d according to blood sugar level) was given for 12 weeks. Fasting blood glucose (FBG), fasting insulin (FINS) and fasting leptin (FLP) were detected by using glucose oxidase method, radioimmunoassay and ELISA, respectively. Insulin sensitivity index (ISI) and homeostasis model assessment-insulin resistance (HOMA-IR) were calculated. In comparison with pre-treatment, FBG levels and HOMA-IR in both acupuncture and medication groups, and FINS and FLP levels in the acupuncture group were decreased significantly (P < 0.01), while ISI in both acupuncture and medication groups, and FINS level in the medication group were increased remarkably after the treatment (P < 0.01). Comparison between two groups showed that after the treatment, FINS and FLP levels, and HOMA-IR of the acupuncture group were considerably lower than those of the medication group (P < 0.01), while ISI of the acupuncture group was significantly higher than that of the medication group (P < 0.01). Acupuncture therapy is effective in lowering FLP level, which may contribute to its clinical effect in improving type-II DM.
Shao J.Q.,Nanjing General Hospital of Nanjing Military Command
Zhonghua yi xue za zhi | Year: 2010
OBJECTIVE: To set up islet perifusion system, a new method to evaluate first-phase insulin secretory function of beta-cell in vitro. METHODS: Islet perifusion system was set up, including perifusion framework, waterbath system, infusion pump system, afferent system, islet capsule, and efferent system. Kinetics of insulin release in vitro was studied using the perifusion system. Pancreatic islets were isolated as mentioned above and used freshly after isolation. Size-matched 50 islets were placed in each column. Then the columns were gently closed with the top adaptors, immersed in vertical position and controlled temperature in the water bath at 37 degrees C. The perifusion medium was maintained at 37 degrees C in a water bath. And all columns were perifused in parallel at a flow rate of 0.5 ml/min with KRBB (2.8 mmol/L glucose) at 37 degrees C. After 60 min static incubation with KRBB (2.8 mmol/L glucose), the islets were stimulated in the continuous presence of a high concentration of 16.7 mmol/L glucose. Samples were collected every 20-second until 2 min, every 1 min until 5 min, thereafter every 5 min until 30 min. Samples were immediately stocked at -80 degrees C until further analysis. Insulin concentration was measured with an insulin RIA kit. RESULTS: An Islet perifusion system was established successfully, and kinetic curves of insulin secretion of db/m and db/db mice were traced out. CONCLUSION: An established islet perifusion system, could be widely used to evaluate insulin secretory function of beta-cell in in vitro research of diabetes.
Zhang Y.,Nanjing General Hospital of Nanjing Military Command
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2013
To study the reference values for differential cell counts in nasal lavage of normality in Nanjing. The total and differential cell counts were examined in nasal lavage from samples of a total of 300 healthy non-smoking adult volunteers. Nasal lavage succeeded in 281 subjects, the achievement ratio was 93.67%. The proportions of eosinophils 0.46 +/- 1.03, neutrophils were 4.46 +/- 9. 84, macrophages 0. 19 +/- 0.73 and lymphocytes 0.04 +/- 0. 16, respectively. There were no significant differences in the differential cell counts between male and female(P>0. 05) . The 95% ceiling percentile of eosinophils and neutrophils in nasal lavage are 2. 58 and 19.76 respectively. The reference values were established for differential cell counts in nasal lavage of normality in Nanjing. We propose that these data be used in research on pathogenesis, diagnosis and treatment of the patients with nasal inflammation.
Yang Z.,Nanjing General Hospital of Nanjing Military Command
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi | Year: 2010
Many functional magnetic resonance imaging (fMRI) studies have revealed the deactivation phenomenon of default mode network in the patients with epilepsy; however, nearly not any of the reports has focused on the dorsal attention network of epilepsy. In this paper, independent component analysis (ICA) was used to isolate the dorsal attention network of 16 patients with temporal lobe epilepsy (TLE) and of 20 healthy normals; and a goodness-of-fit analysis was applied at the individual subject level to choose the interesting component. Intra-group analysis and inter-group analysis were performed. The results indicated that the dorsal attention network included bilateral intraparietal sulcus, middle frontal gyrus, human frontal eye field, posterior lobe of right cerebellum, etc. The TLE group showed decreased functional connectivity in most of the dorsal attention regions with the predominance in the bilateral intraparietal sulcus, middle frontal gyrus, and posterior lobe of right cerebellum. These data suggested that the intrinsic organization of the brain function might be disrupted in TLE. In addition, the decrease of goodness-of-fit scores suggests that activity in the dorsal attention network may ultimately prove a sensitive biomarker for TLE.
DU H.,Nanjing General Hospital of Nanjing Military Command
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2011
To investigate the hypoglycemic action of rhein (4,5-dihydroxyanthraquinone-2-carboxylic acid), one of the anthraquinone derivatives isolated from rhubarb, and study its effects on pancreatic beta-cells in db/db mice. Thirty 4-week-old db/db mice were randomized for an 8-week treatment with intragastric administration of rhein (120 mg/kg, n=15) or placebo (1% natrium cellulose solution, n=15). After the treatment, intraperitoneal glucose tolerance test (IPGTT) was performed and the area under curve (AUC) of insulin levels in IPGTT was calculated to evaluate insulin secretory function. The AUC(INS0-30) was calculated to evaluate the early-phase insulin secretion. Immunohistochemical staining for insulin was performed to estimate the beta-cell mass, and beta-cell apoptosis was detected using TUNEL assay. Compared with the control group, rhein-treated group showed significantly reduced blood glucose concentrations at 0, 30, 60 and 120 min after glucose load with significantly higher insulin levels at 30, 60 and 120 min. The early-phase insulin secretion was also obviously increased. The beta-cell mass was obviously rescued by the 8-week treatment with rhein, which also notably improved the staining intensity of insulin and suppressed beta-cell apoptosis compared with the control. Early rhein treatment significantly improves glucose tolerance by restoring the early-phase insulin secretion in db/db mice and inhibiting the apoptosis of the beta-cells, suggesting the potential of rhein as a novel therapeutic agent for type 2 diabetes.
Liu J.,Nanjing General Hospital of Nanjing Military Command |
Liu J.,Xuzhou Medical College |
Xu Q.,Xuzhou Medical College |
Wang H.,Xuzhou Medical College |
And 3 more authors.
Stroke | Year: 2013
BACKGROUND AND PURPOSE - : Ischemic postconditioning, a brief episode of ischemia after a prolonged ischemic insult, has been found to reduce the delayed neuronal loss after stroke. However, the mechanisms underlying such endogenous neuroprotective strategy remain obscure. In this study, we try to explore the excitatory postsynaptic signal events associated with neuroprotective effect of ischemic postconditioning. METHODS - : Global cerebral ischemia was induced for 15 minutes by the 4-vessel occlusion method in male Sprague-Dawley rats. Ischemic postconditioning was conducted 10 minutes later by a single reocclusion for 3 minutes. RESULTS - : A severe global cerebral ischemia after 5 days of reperfusion destroyed almost all hippocampal CA1 pyramidal neurons. A brief ischemic postconditioning robustly reduced the neuronal loss after ischemia. Preadministration of phosphoinositide 3-kinase inhibitor LY294002 blocked the neuroprotection of postconditioning, whereas mitogen-Activated protein kinase kinase 1 inhibitor PD98059 had no effect. Ischemic postconditioning significantly increased the Akt phosphorylation (Ser473). In addition, postconditioning not only perturbed the binding of postsynaptic density protein-95 with glutamatergic kainate receptor subunit 2 and mixed lineage kinase 3 but also suppressed the downstream activation of mixed lineage kinase 3, mitogen-Activated protein kinase kinase 7, and c-Jun N-terminal kinase 3. LY294002, but not PD98059, abolished the postconditioning-induced decreases in the assembly of glutamatergic kainate receptor subunit 2-postsynaptic density protein-95-mixed lineage kinase 3 complex and in the mixed lineage kinase 3-c-Jun N-terminal kinase 3 signaling. Akt inhibitor IV, a specific Akt inhibitor, showed the same effects as LY294002. CONCLUSIONS - : Ischemic postconditioning protects neurons against stroke by attenuating the postsynaptic glutamatergic kainate receptor subunit 2-postsynaptic density protein-95-mixed lineage kinase 3-c-Jun N-terminal kinase 3 signal cascade via phosphoinositide 3-kinase-Akt pathway. (Stroke. 2013;44:2031-2035.) © 2013 American Heart Association, Inc.
Li Q.,Nanjing General Hospital of Nanjing Military Command |
Hou T.,Chongqing Medical University |
Zhao J.,Nanjing General Hospital of Nanjing Military Command |
Xu J.,Chongqing Medical University
Tissue Engineering - Part A | Year: 2011
Bone tissue engineering has generated promising results in bone defect repair, but is limited by the inherently poor nutrient supply to nonvascularized tissue-engineered bone grafts. In this study, we investigated the delivery of vascular endothelial growth factor (VEGF) and the effect on in vitro cultured human umbilical vein endothelial cells (HUVECs), in an attempt to provide experimental basis for promoting the vascularization of tissue-engineered bone grafts. A mechanical stimulator was developed to generate a periodic compressive load analogous to goat locomotor characteristics, simulating the mechanical stimulation applied on the fracture ends of the load-bearing bone. Poly-l-lysine-coated VEGF/alginate microspheres were combined with demineralized bone matrices into composites, and the in vitro release of VEGF from these composites was evaluated under periodic compression. The effect of the release media on HUVECs was also investigated. Compression slightly accelerated VEGF release at the early stage (<11 days) compared with noncompressed composites, although the release profiles of the two composite groups were generally similar. The released VEGF promoted HUVEC proliferation. In addition, the periodic compression applied on composites containing both HUVECs and VEGF/alginate microspheres promoted the expression of matrix metalloproteinases-2/9 in HUVECs. This study provides a model for investigating VEGF release under simulated in vivo biomechanical conditions and without the disadvantage of the rapid degradation of VEGF in in vivo investigation of VEGF release. The results also provide important guidelines for future in vivo experiments. © Copyright 2011, Mary Ann Liebert, Inc.