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Liu L.,Nanjing General Hospital of Nanjing Military Area Command of PLA | Chen B.,Nanjing General Hospital of Nanjing Military Area Command of PLA | Wu Y.-Y.,Nanjing General Hospital of Nanjing Military Area Command of PLA | Yuan L.,Nanjing General Hospital of Nanjing Military Area Command of PLA | And 3 more authors.
Journal of Interventional Radiology (China) | Year: 2014

Objective: To evaluate CT perfusion imaging in evaluating the changes of the blood vessel function and anatomical morphology in rabbits with VX 2 hind leg muscle transplantation tumor after antiangiogenic drugs via different administration routes, and to discuss the difference in therapeutic efficacy via different administration routes. Methods: A total of 18 hind leg transplantation tumor rabbit models were established. The animals were randomly and equally divided into 3 groups by using digital method. Recombinant human endostatin injection through intravenous therapy was carried out in the rabbits of intravenous group, and local intratumoral injection of recombinant human endostatin was employed in the rabbits of local groups, while local injection of saline was performed in the rabbits of control group. Seven days after the treatment, the VX2 tumor volumes were measured. The vascular function was evaluated by CT perfusion imaging parameters, including blood flow (BF), blood volume (BV) and surface permeability (PS). The results were compared with pathological findings as well as with microvessel density (MVD) which was estimated by immunohistochemical study. Results: The success rate of modeling was 100%. After the treatment, the tumor volumes of intravenous group and local group became smaller than that of control group (P < 0.05). The inhibition rates of intravenous group and local group were 50.52% and 56.21% respectively. Significant tumor necrosis was seen in one rabbit of the local group. Postoperative CT perfusion scanning showed that the perfusion parameters, including BF, BV and PS, in the intravenous group and local group were decreased (P < 0.05). No statistically significant differences in the perfusion parameters existed between the preoperative data and postoperative ones in the control group. The differences in CT perfusion parameters between the intravenous group and the local group were not significant (P > 0.05). The mean values of BF, BV and PS of both the intravenous group and the local group were smaller than those of the control group (P < 0.05). Histopathological examination with HE staining showed that large area of tissue necrosis could be seen in the intravenous group and the local group. In the control group, HE staining showed mild tumor necrosis. Immunohistochemical examination showed that no statistically significant difference in MVD existed between the intravenous group and the local group, although the MVD of the intravenous group and local group was lower than that of the control group (P < 0.05). The CT perfusion parameters were closely correlated with MVD. Conclusion: CT perfusion imaging is an effective and convenient means to assess tumor angiogenesis and vascular function. Local intratumoral injection of anti-angiogenesis drugs is an effective anti-tumor therapy.

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