Wang L.-P.,Nanjing Forestry University |
Ji B.-Z.,Nanjing Forestry University |
Liu S.-W.,Administration Bureau of Dr. Sun Yat Sens Mausoleum |
Zhao Z.-P.,Nanjing Forestry University |
And 2 more authors.
Journal of the Lepidopterists' Society | Year: 2015
The morphology of the reproductive systems and the coupling mechanism between the male and the female genitalia during copulation in Dioryctria rubella Hampson are described. Differing from those in other genera of Phycitinae moths, the corpus bursae possesses a well-developed signum, and the seminal duct does not exhibit a bulla seminalis, instead arising from the ventral surface of the corpus bursae. The ultrastruetural observation shows that the spines on the inner surface of the ductus bursae and the cervix bursa are complementary to that of cornuti on the outer surface of vesica in position and direction, which elucidates the matching mechanism between the ductus bursae and the vesica. The male possesses well-developed scale brushes, which consist of six pairs of overlapped structures. Finally, the morphological differences of scale brushes between D. rubella and other Dioryctria species and related genera, and their function during copulation are discussed.
Yao Y.,Nanjing Agricultural University |
Su J.,Nanjing Agricultural University |
Yang G.,Nanjing Agricultural University |
Zhang G.,Nanjing Agricultural University |
And 6 more authors.
Peptides | Year: 2011
Neuropeptide S (NPS), a newly identified neuropeptide, is involved in many physiological and pathological activities through the NPS receptor (NPSR). Recently, the NPS and NPSR have been detected in peripheral systems of pigs including immune tissues, suggesting that NPS may play an important role in the regulation of immune function. The aim of this study was to demonstrate the presence and function of NPS and NPSR in splenic lymphocytes (SPLs) and pulmonary alveolar macrophages (PAMs) of pigs. By RT-PCR, the expression of NPS and NPSR mRNA was detected in the SPLs and PAMs. NPS immunoreactivity was observed in the membrane and cytoplasm of both SPLs and PAMs. We found that NPS could stimulate the proliferation of SPLs, when NPS was added at concentrations of 0.01, 0.1, 1, 10, 100 and 1000 nM alone or in combination with PHA/LPS in vitro. In macrophages from bronchoalveolar lavage (BAL) fluid of pigs, various doses of NPS (0.01, 0.1, 1, 10, 100 and 1000 nM) up-regulated the phagocytosis of PAMs in comparison to controls. In PAMs, NPS could induce the production of the pro-inflammatory cytokines IL-1β, IL-6 and TNF-α. Taken together, all data suggest that NPS is capable of inducing phagocytosis of non-opsonized E. coli. NPS might act as potent neuroimmunomodulatory factors and affects the maintenance of immune homeostasis. © 2010 Elsevier Inc. All rights reserved.
Wang G.-L.,Jiangnan University |
Xu X.-F.,Jiangnan University |
Cao L.-H.,Nanjing Entry Exit Inspection and Quarantine Bureau |
He C.-H.,Nanjing Entry Exit Inspection and Quarantine Bureau |
And 2 more authors.
RSC Advances | Year: 2014
Research on oxidase mimics is challenging but important. In this article, mercury(ii) ion enabled citrate-capped silver nanoparticles (Cit-AgNPs) exhibit catalytic activity toward the oxidation of typical chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) by dissolved oxygen under mild conditions, suggesting a new type of oxidase mimic. In addition, the oxidase-like activity of Cit-AgNPs was sensitive to the concentration of mercury(ii) ions and selective towards mercury(ii) ions among other metal ions. Based on this, a facile colorimetric mercury(ii) ion sensor was developed. Hg2+ was reduced on the surface of Cit-AgNPs to form Hg-Ag alloys. The Hg-Ag alloys activated oxygen and generated superoxide anions, which oxidized TMB. This discovery indicates the potential of nanoparticles for efficient enzyme mimetics. © 2014 The Royal Society of Chemistry.
Gao H.,Innerspec Technologies Inc. |
Lopez B.,Innerspec Technologies Inc. |
Minguez X.,Innerspec Technologies Europe |
Chen J.,Nanjing Entry Exit Inspection and Quarantine Bureau
Proceedings of 2015 IEEE Far East NDT New Technology and Application Forum, FENDT 2015 | Year: 2015
Weld inspection is a recurring topic in nondestructive testing because of the potential safety and environmental impact associated to poor weld quality. Thick-wall, multi-pass welds are very common in construction, bridge building, ship building, pressure vessel, and many other industries. This paper presents a novel technique to provide process and quality control of a partially completed weld after each individual weld pass, which can provide significant savings in time and money. The technique uses electromagnetic acoustic transducer (EMAT) to generate surface acoustic waves that detect defects on the top-most layer of a multi-pass weld. The paper describes the theory and empirical verification on a partially finished weld with 100mm thick base metal. © 2015 IEEE.
Tian Y.,Nanjing Agricultural University |
Liu D.,Nanjing Agricultural University |
Zhao Y.,CAS Institute of Botany |
Wu J.,Nanjing Entry Exit Inspection and Quarantine Bureau |
And 3 more authors.
European Journal of Plant Pathology | Year: 2016
A method was developed using a Loop-mediated isothermal amplification assay (LAMP) for detecting Didymella bryoniae in cucurbit seeds. The LAMP primers were designed based on the DNA-dependent RNA polymerase II RPB140 gene (RPB2) from D. bryoniae. Calcein was used as an indicator for the endpoint visual detection of DNA amplification. The LAMP assay was conducted in isothermal (65 °C) conditions within 1 h. The detection threshold of the LAMP assay was 10 pg of genomic DNA and D. bryoniae was detected in 100 % of artificially infested seedlots with 0.05 % infestation or greater. With the LAMP assay, 16 of 60 watermelon and muskmelon seedlots collected from Xinjang province were determined to be positive for D. bryoniae. In contrast, a real-time PCR assay determined that 11 of the 60 seedlots from Xinjiang province were positive for the pathogen. These results showed that the LAMP technique was simple, rapid and well suited for detecting D. bryoniae DNA, especially in seed health testing. © 2016 Koninklijke Nederlandse Planteziektenkundige Vereniging
Gao H.,Huazhong University of Science and Technology |
Wang D.,Huazhong University of Science and Technology |
Zhang S.,Huazhong University of Science and Technology |
Xu M.,Huazhong University of Science and Technology |
And 8 more authors.
Regulatory Toxicology and Pharmacology | Year: 2015
3-methyl-2-quinoxalin benzenevinylketo-1, 4-dioxide (Quinocetone, QCT) has been broadly used to treat dysentery and promote animal growth in food producing animals. However, its potential toxicity could not been neglected as parts of safety assessment according to the acceptable guidelines for QCT administration. In this study, the immunotoxicity of QCT was investigated in male Sprague-Dawley (SD) rats following a 28-day oral exposure at doses of 0, 50, 800, and 2400 mg/kg/day. The food consumption, body weight gain and relative spleen weight were significantly decreased by QCT in a dose-dependent manner. Treatment of rats with QCT also notably suppressed the T-cell proliferation and natural killer (NK) cell activity, accompanied by intracellular reactive oxygen species (ROS) accumulation, antioxidant system inhibition and DNA damage enhancement. Thus, the primary finding of this study is that QCT exposure (2400 mg/kg/day) could cause immunotoxicity in SD rats due to ROS mediated oxidative stress and DNA damage. © 2015 Elsevier Inc.
Yao Y.,Nanjing Agricultural University |
Yao Y.,Nanjing Institute of Zootechnics and Poultry Science |
Su J.,Nanjing Agricultural University |
Zhang F.,Nanjing Entry Exit Inspection and Quarantine Bureau |
Lei Z.,Nanjing Agricultural University
NeuroImmunoModulation | Year: 2013
Objectives: The recently discovered neuropeptide S (NPS) and its cognate receptor represent a novel system of neuromodulation and are involved in many physiological and pathological processes. NPS has been implicated in the regulation of proinflammatory cytokine secretion in the pulmonary alveolar macrophages (PAMs) of pigs in vitro. In this study, we tested the hypothesis whether either central or peripheral injection of NPS would stimulate the secretion of the proinflammatory cytokines in pigs. Methods: In experiment 1, pigs were fitted with an intracerebroventricular cannula and indwelling jugular catheters, and were then randomly assigned to receive 10 or 30 nmol NPS in artificial cerebrospinal fluid. In experiment 2, pigs were fitted with indwelling jugular catheters, and randomly received 15 or 30 nmol NPS in saline. Serial blood samples were collected every 10 min for 1 h before and for 2 h after injections, and serum concentrations of IL-1β, IL-6 and TNF- were determined. Results: Serum concentrations of these cytokines were increased in pigs that received central and peripheral injection of NPS, and the elevated secretion of these cytokines was in a time- and concentration-dependent manner. Conclusion: The level of serum proinflammatory cytokines could be activated by both central and peripheral administration of NPS in a dose- and time-dependent manner in the pig. The present data support the concept that NPS may be considered as a potent modulator for the immune system and may play an important role in the inflammation and immune system of pigs. © 2013 S. Karger AG, Basel.
PubMed | Chinese Academy of Agricultural Sciences, Nanjing Entry Exit Inspection and Quarantine Bureau, Emory University and Huazhong University of Science and Technology
Type: | Journal: Brain, behavior, and immunity | Year: 2016
Aging is a principal risk factor for neurodegenerative diseases and especially shares similar pathologic mechanisms to Alzheimers disease (AD). Amyloid- (A) plaques deposition and neurofibrillary tangles (NFTs) are the prominent age-dependent pathologies implicated in the cognitive deficits. Accumulation of mis-folded proteins in the endoplasmic reticulum triggers a cellular stress response called the unfolded protein response (UPR), the activation of which is increased in AD patients. However, the UPR relates to the pathological hallmarks of aging is still elusive. In this study, we report that long-term supplement of -linolenic acid (ALA), starting before the onset of disease symptoms (6month-old), prevents the age-related memory deficits during natural aging. The amelioration of the memory impairment is associated with a decrease in UPR related markers [glucose regulated protein 78 (GRP78), protein kinase RNA-like endoplasmic reticulum kinase (PERK), eukaryotic Initiation Factor 2 (eIF2)]. ALA suppressed the PERK/eIF2 signaling, which may be responsible for multifaceted memory-deteriorating and neurodegenerative mechanisms, including inhibition of A production by suppressing -site APP-cleaving enzyme 1 (BACE1) expression, enhancement of cAMP response element binding protein (CREB) function via down-regulating activating transcription factor 4 (ATF4), and suppression of Tau phosphorylation by inhibiting glycogen synthase kinase 3 (GSK-3) pathway. Taken together, our findings provide new insights into the link between ALA and PERK/eIF2 signaling, which could contribute to a better understanding of an ALA-mediated protective effect in aging-associated neuropathology.
PubMed | Nanjing Entry Exit Inspection and Quarantine Bureau and Purdue University
Type: Journal Article | Journal: ACS applied materials & interfaces | Year: 2016
Fabricating portable devices for the determination of heavy metal ions is an ongoing challenge. Here, a 3D printing approach was adopted to fabricate a microfluidic electrochemical sensor with the desired shape in which the model for velocity profiles in microfluidic cells was built and optimized by the finite element method (FEM). The electrode in the microfluidic cell was a flexible screen-printed electrode (SPE) modified with porous Mn
PubMed | Nanjing Entry Exit Inspection and Quarantine Bureau and Huazhong University of Science and Technology
Type: Journal Article | Journal: Regulatory toxicology and pharmacology : RTP | Year: 2015
3-methyl-2-quinoxalin benzenevinylketo-1, 4-dioxide (Quinocetone, QCT) has been broadly used to treat dysentery and promote animal growth in food producing animals. However, its potential toxicity could not been neglected as parts of safety assessment according to the acceptable guidelines for QCT administration. In this study, the immunotoxicity of QCT was investigated in male Sprague-Dawley (SD) rats following a 28-day oral exposure at doses of 0, 50, 800, and 2400 mg/kg/day. The food consumption, body weight gain and relative spleen weight were significantly decreased by QCT in a dose-dependent manner. Treatment of rats with QCT also notably suppressed the T-cell proliferation and natural killer (NK) cell activity, accompanied by intracellular reactive oxygen species (ROS) accumulation, antioxidant system inhibition and DNA damage enhancement. Thus, the primary finding of this study is that QCT exposure (2400 mg/kg/day) could cause immunotoxicity in SD rats due to ROS mediated oxidative stress and DNA damage.