Nanjing, China
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Tan X.,Nanjing Dachang Hospital | He X.,Red Cross | Jiang Z.,Nanjing Medical University | Wang X.,Nanjing Medical University | And 4 more authors.
Molecular and Cellular Biochemistry | Year: 2015

Derlin-1 is overexpressed in many types of solid tumors and plays an important role in cancer progression. However, the expression pattern and functions of Derlin-1 in human colon cancer are not fully understood. In the present study, we examined Derlin-1 expression in colon cancer cell lines and human tissues and investigated its role in colon cancer. We found that Derlin-1 expression was increased significantly in colon cancer tissues and its overexpression correlated with the tumor differentiation, Dukes stage, invasion, lymph node metastasis, distant metastasis, and poor overall survival. The silencing of Derlin-1 by shRNA led to the growth inhibition of colon cancer cells, which were associated with the promotion of apoptosis. Furthermore, Derlin-1 silencing significantly inhibited the activation of the PI3K/AKT signaling pathway. Taken together, our results showed that Derlin-1 is overexpressed in colon cancer and promotes proliferation of colon cancer cells. Derlin-1 may be a potential therapeutic target for the treatment of colon cancer. © 2015, Springer Science+Business Media New York.

Wang L.,Nanjing University | Chen Y.,Nanjing Dachang Hospital | Qian J.,Nanjing University | Tan Y.,Nanjing University | And 4 more authors.
Journal of Mechanics in Medicine and Biology | Year: 2013

Tissue engineering is a promising approach to regenerate transplantable tissue or organ substitutes in vitro. However, the existing methods are based on seeding cells on macroscale polymer scaffolds, which are associated with several challenges including limited control over cell microenvironment, limited nutrient diffusion, directed cell alignment. The emerging bottom-up tissue engineering methods hold great potential to address these challenges by assembling building blocks into complex 3D tissue constructs. In this study, we developed a layer-by-layer assembly approach to recreate 3D cell-laden constructs. Our experiment showed the predefined channels form a vascular system and help the transplant cells to transport the requirement of culture cells in early case of cells attaching and growing up. It is an original concept to demonstrate the feasibility of forming a network with a vascular geometry in a biocompatible polymer and fabricated different scaffold with different cells. The concept was developed to create a complete branching vascular circulation in 3D on surface of mixture of chitosan and gelatin structures and pre-define the structure of channel for culturing smooth muscle for controlling the SMC growing up as smooth muscle fiber. © World Scientific Publishing Company.

Jia B.,Nanjing Dachang Hospital | Tian Z.-M.,Nanjing Dachang Hospital | Nie H.-C.,Nanjing Dachang Hospital | Wu C.-J.,Nanjing Dachang Hospital
Journal of Interventional Radiology | Year: 2010

Objective: To discuss the therapeutic effects of oxygen-ozone injection into both the nucleus pulposus and lateral recess in treating lumbar disc herniation. Methods Eighty-eight patients with CT-proved or MRI-proved lumbar disc herniation were used as study group, who received the treatment of percutaneous oxygen-ozone injection into both the nucleus pulposus and lateral recess. Another 74 patients with lumbar disc herniation were used as control group, who received percutaneous oxygen-ozone injection into the nucleus pulposus only. Results After the treatment, the patients in the study group were relived from their symptoms much faster and the clinical effect was kept much longer. At 6 month after the procedure, the clinical therapeutic efficiency of study group was 84.1%. The effective rates of study group at 1, 2 and 6 months after the operation were much higher than those of control group, the differences between two groups were statistically significant (χ2 = 4.09, P = 0.043; χ2 = 4.62, P = 0.032; χ2 = 4.38, P = 0.036, respectively). Conclusion For the treatment of lumbar disc herniation percutaneous oxygen-ozone injection into both the nucleus pulposus and lateral recess is more effective than injection into nucleus pulposus only.

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