Nanhai Entry Exit Inspection and Quarantine Bureau

Foshan, China

Nanhai Entry Exit Inspection and Quarantine Bureau

Foshan, China
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Wang Y.,South China Agricultural University | Chen G.,South China Agricultural University | Liang J.,University Putra Malaysia | Zou Y.,Nanhai Entry Exit Inspection and Quarantine Bureau | And 3 more authors.
Environmental Science and Pollution Research | Year: 2015

Using manure collected from swine fed with diet containing antibiotics and antibiotic-free swine manure spiked with antibiotics are the two common methods of studying the degradation behavior of veterinary antibiotic in manure in the environment. However, few studies had been conducted to co-compare these two different antibiotic addition methods. This study used oxytetracycline (OTC) as a model antibiotic to study antibiotic degradation behavior in manure under the above two OTC addition methods. In addition, the role of microorganisms present in the manure on degradation behavior was also examined. The results showed that degradation half-life of OTC in manure from swine fed OTC (9.04 days) was significantly shorter than that of the manure directly treated with OTC (9.65 days). Concentration of 4-epi-OTC in manure from swine fed OTC peaked earlier than that in manure spiked with OTC, and the degradation rates of 4-epi-OTC and α-apo-OTC in the manure from swine fed OTC were faster, but the peak concentrations were lower, than those in manure spiked with OTC. Bacterial diversity and relative abundance of Bacillus cereus data demonstrated that sterilization of the manure before experiment significantly decreased OTC degradation rate in both of the addition methods. Results of the present study demonstrated that the presence of the metabolites (especially 4-epi-OTC) and microorganisms had significant influence on OTC degradation. © 2015 Springer-Verlag Berlin Heidelberg


Tan J.,Central South University | Zhang T.,Central South University | Jiang L.,Nanhai Entry Exit Inspection and Quarantine Bureau | Chi J.,Central South University | And 5 more authors.
Journal of Biological Chemistry | Year: 2011

Mutations in the ATP13A2 gene are associated with Kufor-Rakeb syndrome (KRS) and are found also in patients with various other types of parkinsonism. ATP13A2 encodes a predicted lysosomal P5-type ATPase that plays important roles in regulating cation homeostasis. Disturbance of cation homeostasis in brains is indicated in Parkinson disease pathogenesis. In this study, we explored the biological function of ATP13A2 as well as the pathogenic mechanism of KRS pathogenic ATP13A2 mutants. The results revealed that wild-type ATP13A2, but not the KRS pathogenic ATP13A2 mutants, protected cells from Mn2+-induced cell death in mammalian cell lines and primary rat neuronal cultures. In addition, wild-type ATP13A2 reduced intracellular manganese concentrations and prevented cytochrome c release from mitochondria compared with the pathogenic mutants. Furthermore, endogenous ATP13A2 was up-regulated upon Mn2+ treatment. Our results suggest that ATP13A2 plays important roles in protecting cells against manganese cytotoxicity via regulating intracellular manganese homeostasis. The study provides a potential mechanism of KRS and parkinsonism pathogenesis. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc.


Chen G.-X.,South China Agricultural University | He W.-W.,South China Agricultural University | Wang Y.,South China Agricultural University | Zou Y.-D.,Nanhai Entry Exit Inspection and Quarantine Bureau | And 3 more authors.
Science of the Total Environment | Year: 2014

The degradation behavior of veterinary antibiotics in soil is commonly studied using the following methods of adding antibiotics to the soil: (i) adding manure collected from animals fed with a diet containing antibiotics, (ii) adding antibiotic-free animal manure spiked with antibiotics and (iii) directly adding antibiotics. No research simultaneously comparing different antibiotic addition methods was found. Oxytetracycline (OTC) was used as a model antibiotic to compare the effect of the three commonly used antibiotic addition methods on OTC degradation behavior in soil. The three treatment methods have similar trends, though OTC degradation half-lives show the following significant differences (P. <. 0.05): manure from swine fed OTC (treatment A). <. antibiotic-free manure. +. OTC (treatment B). <. OTC (treatment C). Differences could be caused by distinct chemical reaction equilibria due to dissimilar concentrations of 4-epi-OTC and α-apo-OTC. The pH could also have affected the concentration of 4-epi-OTC and α-apo-OTC, thus influencing OTC degradation. The treatments presenting manure (A and B) significantly enhanced EC, enzyme activity, microbial biomass carbon and nitrogen when compared to the treatment without manure (C), thus increasing degradation of OTC in the soil. Because the main entry route for veterinary antibiotics into soil is via the manure of animals given with antibiotics, the most appropriate method to study the degradation and ecotoxicity of antibiotic residues in soil may be to use manure from animals that are given a particular antibiotic, rather than by adding it directly to the soil. © 2014 Elsevier B.V.


Wang X.,South China Agricultural University | Guo R.,South China Agricultural University | Ma B.,Nanhai Entry Exit Inspection and Quarantine Bureau | Liang J.,University Putra Malaysia | And 2 more authors.
Environmental Progress and Sustainable Energy | Year: 2014

This study examined the fate and effects of tylosin (TYL) on anaerobic digestion of swine wastewater at two concentrations of tylosin A (TYLA) using manure collected from swine fed with TYLA (TYLFED) or direct addition of TYLA (TYLADD) to antibiotic-free swine manure, to examine whether there are differences between the two TYL addition methods on methane production and other fermentation parameters. TYLA and tylosin D (TYLD) concentrations, pH, Chemical oxygen demand (COD), Total nitrogen (TN), and diversity of methanogenic archaea population were parameters used for this study. The results showed that concentrations of TYLA and TYLD in the TYL FED treatment were higher (P < 0.05) than those in the TYL ADD treatment. Methane production in the TYLFEDH and TYLFEDL was reduced by 35.52% and 37.06%, respectively, compared with the control during the 7 days addition period; but for TYLADDH and TYLADDL, the decrease (P < 0.05) was only 12.98% and 7.84%, respectively. The diversity index of methanogenic archaea in the TYL FED treatment were lower than that in the control and the TYL ADD on Day 4, but no difference (P > 0.05) between the control and the TYLADD treatment was observed. pH, COD, and TN were not affected by treatment. Our results showed clear differences in the rate of methane inhibition by the two antibiotic inclusion methods; been higher for the TYL FED treatment than the TYLADD treatment. Since the former method resembles more under actual farm conditions, we suggested that studies on effects of antibiotic residues on anaerobic fermentation should adopt such an approach. © 2013 American Institute of Chemical Engineers.


Huang L.,South China Agricultural University | Guo R.,South China Agricultural University | Liang J.,University Putra Malaysia | Ma B.,Nanhai Entry Exit Inspection and Quarantine Bureau | And 2 more authors.
Asian Journal of Chemistry | Year: 2014

Veterinary antibiotic residue in manure is one of the most important factors which have an impact on the anaerobic digestion. This article studied the effect of sulfamethazine (SM-2) on psychrophilic anaerobic digestion of swine manure by two adding methods, addition of manure from swine fed with SM-2 in diet and direct addition of SM-2 with blank manure, under two adding concentrations of SM-2 (2.38 and 1.24 mg/g dry swine manure). Anaerobic digestion experiment was conducted at 20 °C in laboratory digesters with the effective working volume of 1.3 L. Methane production of each digester was measured by displacement method daily. Grad samples were collected to determine the concentration of SM-2, pH, chemical oxygen demand (COD) in slurry and organic matter in biogas residue. The results showed that the concentration of SM-2 in anaerobic system fed with manure containing SM-2 was lower than that fed with blank manure and SM-2 under the same addition of SM-2 and the degradation rate of SM-2 in the high concentration treatment was faster than that in the low concentration treatment. Compared with the control group, the treatments added with SM-2 had no significant difference in methane production, COD in slurry and organic matter in biogas residue, but adding SM-2 and blank manure improved pH of swine slurry in SM-2 adding period. Due to the different degradation rate of SM-2 in anaerobic digestion between the two adding methods of SM-2, using manure collect from animals administered with SM-2 is more appropriate to assess the effect of SM-2 on anaerobic digestion.


Peng P.-C.,South China Agricultural University | Wang Y.,South China Agricultural University | Liu L.-Y.,South China Agricultural University | Zou Y.-D.,Nanhai Entry Exit Inspection and Quarantine Bureau | And 4 more authors.
Poultry Science | Year: 2016

The excretion rates and ecological risk to the environment of three commonly used veterinary antibiotics (VAs), amoxicillin, ciprofloxacin, and doxycycline, in layer hen manure during the application and withdrawal periods were investigated in a study consisting of a control group fed with VA-free basal diet and nine treatment groups consisted of three levels (200 mg/kg, 100 mg/kg, and 50 mg/kg) of amoxicillin (AMX), ciprofloxacin (CIP), or doxycycline (DOC). Each treatment group was replicated seven times with three layer hens per replication. Results of the study showed that the average excretion rates of AMX in the 200, 100, and 50 mg/kg groups were 67.88, 55.82, and 66.15%, respectively, while those for CIP and DOC were 47.84, 51.85, and 44.87% and 82.67, 94.39, and 95.72%, respectively. The concentrations of the above veterinary drugs in manure decreased sharply in the withdrawal period (7, 28, and 10 d, respectively), for AMX, DOC, and CIP. Neither AMX nor DOC was detected in the manure after the withdrawal period. In contrast to AMX and DOC, the excretion rate of CIP was significantly lower and thus had a longer residence time. Ecological risk study, estimated using hazard quotient values, showed that AMX in the 100 and 50 mg/kg groups posed no risk to the environment after d 1 of withdrawal, while CIP in the 50 mg/kg group posed no risk to the environment from d 5 of withdrawal. CIP in the 200 and 100 mg/kg groups required 10 d withdrawal in order to pose no risk to the environment. In contrast, DOC residue during withdrawal in the manure posed no risk to the environment, thus making it more environmentally safe. © 2016 Poultry Science Association Inc.


Li Q.,South China Agricultural University | Wang Y.,South China Agricultural University | Zou Y.-D.,Nanhai Entry Exit Inspection and Quarantine Bureau | Liao X.-D.,South China Agricultural University | And 3 more authors.
Science of the Total Environment | Year: 2015

The behavior of veterinary antibiotics in the soil is commonly studied using the following methods to add antibiotics to the soil: (A) adding manure collected from animals fed a diet that includes antibiotics; (B) adding antibiotic-free animal manure spiked with antibiotics; and (C) the direct addition of antibiotics. However, most studies have only used methods (B) and (C) in their research, and few studies have simultaneously compared the different antibiotic addition methods. This study used tylosin A (TYLA) as a model antibiotic to compare the effects of these three commonly used antibiotic addition methods on the dissipation rates of TYLA and the numbers of resistance genes in laboratory incubation experiments. The results showed that the three treatment methods produced similar TYLA degradation trends; however, there were significant differences (P<0.05) in the TYLA degradation half-life (t1/2) among the three methods. The half-life of TYLA degradation in treatments A, B and C was 2.44±0.04, 1.21±0.03 and 5.13±0.11days, respectively. The presence of manure resulted in a higher electrical conductivity (EC), higher relative abundance of Citrobacter amalonaticus, higher macrolide resistant gene (ermB, ermF and ermT) count and lower ecological toxicity in the soil, which could partially explain the higher TYLA degradation rate in the treatments containing manure. The higher degradation rate of TYLA in treatment B when compared to treatment A could be due to the lower concentrations of tylosin B (TYLB) and tylosin D (TYLD). The main route for veterinary antibiotics to enter the soil is via the manure of animals that have been administered antibiotics. Therefore, the more appropriate method to study the degradation and ecotoxicity of antibiotic residues in the soil is by using manure from animals fed/administered the particular antibiotic rather than by adding the antibiotic directly to the soil. © 2015.


PubMed | University Putra Malaysia, South China Agricultural University and Nanhai Entry Exit Inspection and Quarantine Bureau
Type: Journal Article | Journal: Journal of environmental sciences (China) | Year: 2014

Effects of antibiotic residues on methane production in anaerobic digestion are commonly studied using the following two antibiotic addition methods: (1) adding manure from animals that consume a diet containing antibiotics, and (2) adding antibiotic-free animal manure spiked with antibiotics. This study used chlortetracycline (CTC) as a model antibiotic to examine the effects of the antibiotic addition method on methane production in anaerobic digestion under two different swine wastewater concentrations (0.55 and 0.22mg CTC/g dry manure). The results showed that CTC degradation rate in which manure was directly added at 0.55mg CTC/g (HSPIKE treatment) was lower than the control values and the rest of the treatment groups. Methane production from the HSPIKE treatment was reduced (p<0.05) by 12% during the whole experimental period and 15% during the first 7days. The treatments had no significant effect on the pH and chemical oxygen demand value of the digesters, and the total nitrogen of the 0.55mg CTC/kg manure collected from mediated swine was significantly higher than the other values. Therefore, different methane production under different antibiotic addition methods might be explained by the microbial activity and the concentrations of antibiotic intermediate products and metabolites. Because the primary entry route of veterinary antibiotics into an anaerobic digester is by contaminated animal manure, the most appropriate method for studying antibiotic residue effects on methane production may be using manure from animals that are given a particular antibiotic, rather than adding the antibiotic directly to the anaerobic digester.


PubMed | University Putra Malaysia, South China Agricultural University and Nanhai Entry Exit Inspection and Quarantine Bureau
Type: Comparative Study | Journal: Environmental science and pollution research international | Year: 2015

Using manure collected from swine fed with diet containing antibiotics and antibiotic-free swine manure spiked with antibiotics are the two common methods of studying the degradation behavior of veterinary antibiotic in manure in the environment. However, few studies had been conducted to co-compare these two different antibiotic addition methods. This study used oxytetracycline (OTC) as a model antibiotic to study antibiotic degradation behavior in manure under the above two OTC addition methods. In addition, the role of microorganisms present in the manure on degradation behavior was also examined. The results showed that degradation half-life of OTC in manure from swine fed OTC (9.04 days) was significantly shorter than that of the manure directly treated with OTC (9.65 days). Concentration of 4-epi-OTC in manure from swine fed OTC peaked earlier than that in manure spiked with OTC, and the degradation rates of 4-epi-OTC and -apo-OTC in the manure from swine fed OTC were faster, but the peak concentrations were lower, than those in manure spiked with OTC. Bacterial diversity and relative abundance of Bacillus cereus data demonstrated that sterilization of the manure before experiment significantly decreased OTC degradation rate in both of the addition methods. Results of the present study demonstrated that the presence of the metabolites (especially 4-epi-OTC) and microorganisms had significant influence on OTC degradation.


PubMed | University Putra Malaysia, South China Agricultural University and Nanhai Entry Exit Inspection and Quarantine Bureau
Type: | Journal: The Science of the total environment | Year: 2014

The degradation behavior of veterinary antibiotics in soil is commonly studied using the following methods of adding antibiotics to the soil: (i) adding manure collected from animals fed with a diet containing antibiotics, (ii) adding antibiotic-free animal manure spiked with antibiotics and (iii) directly adding antibiotics. No research simultaneously comparing different antibiotic addition methods was found. Oxytetracycline (OTC) was used as a model antibiotic to compare the effect of the three commonly used antibiotic addition methods on OTC degradation behavior in soil. The three treatment methods have similar trends, though OTC degradation half-lives show the following significant differences (P<0.05): manure from swine fed OTC (treatment A)

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