Nandha Arts and Science College
Nandha Arts and Science College
PubMed | Ton Duc Thang University, Daegu University, Nandha Arts and Science College, Japan National Institute of Materials Science and Bharathidasan University
Type: | Journal: Enzyme and microbial technology | Year: 2016
The synthesis of nanoparticles (NP) using algae has been underexploited and even unexplored. In recent times, there are few reports on the synthesis of NP using algae, which are being used as a bio-factory for the synthesis. Moreover, the algae are a renewable source, so that it could be effectively explored in the green synthesis of NP. Hence, this review reports on the biosynthesis of NP especially gold and silver NP using algae. The most widely reported NP from algae are silver and gold than any other metallic NP, which might be due to their enormous biomedical field applications. The NP synthesized by this method is mainly in spherical shape; the reports are revealing the fact that the cell free extracts are highly exploited for the synthesis than the biomass, which is associated with the problem of recovering the particles. Besides, mechanism involving in the reduction and stabilization is well demonstrated to deepen the knowledge towards enhancement possibilities for the synthesis and applications.
Sahayaraj K.,Crop Protection Research Center |
Roobadevi M.,Nandha Arts and Science College |
Rajesh S.,Crop Protection Research Center |
Azizi S.,University Putra Malaysia
Research on Chemical Intermediates | Year: 2015
Noble-metal nanomaterials are of particular interest today because of their applications in many areas, including agriculture. The latter topic is one of the most active areas of research in metal nanomaterials. Metal nanoparticles are traditionally synthesized by wet chemical techniques, in which the chemicals used are often toxic and flammable. We report here biosynthesis of silver nanoparticles using leaf extract of Vernonia cinerea (L.) Less. (Asteraceae). Treatment of aqueous solution of AgNO3 with V. cinerea leaf extract resulted in rapid formation of stable silver nanoparticles. The growth of nanoparticles was monitored by UV-Visible spectrophotometry complemented by characterization using transmission electron microscopy (TEM), X-ray diffraction analysis, and Fourier-transform infrared spectroscopy. A feasible mechanism for the formation of nanomaterial and the difference in the reduction time for silver nanoparticle synthesis is discussed. TEM analysis revealed the presence of polydisperse silver nanoparticles with average size of 5-50 nm. X-ray diffraction studies corroborated that the biosynthesized nanoparticles were crystalline silver. Furthermore, this green biogenic approach is a rapid and simple alternative to chemical synthesis. The biologically synthesized silver nanoparticles were found to be highly effective against Xanthomonas campestris pv. malvacearum (13.00 ± 0.58 mm) with minimum inhibitory concentration of 80 μg/mL. Hence, such biosynthesized silver nanoparticles can be used in control of cotton bacterial blight. © 2014 Springer Science+Business Media.
Santhosh Kumar P.,Sri Venkateswara Institute of Medical Sciences |
Santhosh Kumar P.,Nandha Arts and Science College |
Jayanthi B.,Nandha Arts and Science College |
Abdul K.,Nandha Arts and Science College |
And 3 more authors.
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2012
Cefuroxime axetil (CFA) is the oral prodrug formulation of the injectable antibiotic cefuroxime sodium. It is the second generation cephalosporin with both an intravenous and oral formulation. CFA is the 1-acetoxyethyl ester of cefuroxime. A rapid and reproducible High Performance Liquid Chromatographic method has been developed for the estimation of CFA in its pure form as well as in pharmaceutical dosage forms. Chromatography was carried out on an ODS C18column (150 x 4.6 mm x 5 μm length), using a mixture of methanol and 0.01M potassium dihydrogen orthophosphate buffer (pH-2.0±0.05) (60:40 v/v) as the mobile phase at a flow rate of 0.8 mL/min and the detection was done at 248 nm was developed and fully validated for the determination of CFA. The retention time of the drug was 3.693 min. The method produced linear responses in the concentration range of 0.45 to 80 μg/mL of CFA. Developed HPLC method was sensitive with LOD= 0.26 μgmL-1 and LOQ= 0.58 μgmL-1. The method was successfully validated in accordance to ICH guidelines and was found to be reproducible for analysis of the drug in parental preparations.