Nanchong Institute of Agricultural science

Nanchong, China

Nanchong Institute of Agricultural science

Nanchong, China
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Zheng Z.P.,Nanchong Institute of Agricultural science | Liu X.H.,China West Normal University | Huang Y.B.,Sichuan Agricultural University | Wu X.,Nanchong Institute of Agricultural science | And 2 more authors.
Genetics and Molecular Research | Year: 2012

Days to silking (DTS) is one of the most important traits in maize (Zea mays). To investigate its genetic basis, a recombinant inbred line population was subjected to high and low nitrogen (N) regimes to detect quantitative trait loci (QTLs) associated with DTS. Three QTLs were identified under the high N regime; these explained 25.4% of the phenotypic variance. Due to additive effects, the QTL on chromosome 6 increased DTS up to 0.66 days; while the other two QTLs mapped on chromosome 9 (one linked with Phi061 and the other linked with Nc134) decreased DTS 0.89 and 0.91 days, respectively. Under low N regime, two QTLs were mapped on chromosomes 6 and 9, which accounted for 25.9% of the phenotypic variance. Owing to additive effects, the QTL on chromosome 6 increased DTS 0.67 days, while the other QTL on chromosome 9 decreased it 1.48 days. The QTL on chromosome 6, flanked by microsatellite markers Bnlg1600 and Phi077, was detectedchromosome 6 and three on chromosome 9. These results contribute to our understanding of the genetic basis of DTS and will be useful for developing marker-assisted selection in maize breeding programs. ©FUNPEC-RP.


Zhang K.,Southwest University | Zhang K.,Sweet Potato Engineering and Technology Research Center | Wu Z.-D.,Southwest University | Li Y.-H.,Southwest University | And 13 more authors.
Journal of Integrative Agriculture | Year: 2014

To determine the genetic diversity and population structure of sweet potato accessions cultivated in China, and to establish the genetic relationships among their germplasm types, a representative collection of 240 accessions was analyzed using inter-simple sequence repeat (ISSR) markers. The mean genetic similarity coefficient, Nei's gene diversity, and shared allele distance of tested sweet potato accessions were 0.7302, 0.3167 and 0.2698, respectively. The 240 accessions could be divided into six subgroups and five subpopulations based on neighbor-joining (NJ) clustering and STRUCTURE results, and obvious genetic relationships among the tested sweet potato accessions were identified. The marker-based NJ clustering and population structure showed no distinct assignment pattern corresponding to flesh color or geographical ecotype of the tested sweet potato germplasm. Analysis of molecular variance (AMOVA) revealed small but significant difference between white and orange-fleshed sweet potato accessions. Small but significant difference were also observed among sweet potato accessions from the Southern summer-autumn sweet potato region, the Yellow River Basin spring and summer sweet potato region and the Yangtze River Basin summer sweet potato region. This study demonstrates that genetic diversity in the tested sweet potato germplasm collection in China is lower than that in some reported sweet potato germplasm collections from other regions. Pedigree investigations suggest that more diverse Chinese sweet potato varieties should be formed by broadening the selection scope of breeding parents and incorporating the introduced varieties into future breeding programs. © 2014 Chinese Academy of Agricultural Sciences.


Liu X.H.,China West Normal University | He S.L.,Nanchong Institute of Agricultural science | Zheng Z.P.,Nanchong Institute of Agricultural science | Huang Y.B.,Sichuan Agricultural University | And 2 more authors.
Maydica | Year: 2010

Row number per ear (RNPE) and grain number per row (GNPR) were two very important agronomic traits in maize (Zea mays L.), related to yield. To understand their genetic basis, an F 9 recombinant inbred line (RIL) population and two nitrogen (N) regimes were used to map and characterize the quantitative trait loci (QTLs) associated with RNPE and GNPR. As a result, three QTLs for RNPE were simultaneously detected under both N conditions, on chromosomes 4 (two) and 9 (one). The three identified QTLs could explain 44.55 and 43.26% of phenotypic variation under high and low N regimes, respectively. Among which, the one linked with Umc2188 (bin4.08) on chromosome 4 had negative additive effect, while, the other two possessed positive additive effects. For the trait GNPR, total five QTLs were mapped on chromosomes 1 (one), 2 (one), 6 (two) and 9 (one), of which the two near to Bnlg439 (bin1.03) and Umc1490 (bin6.07) were detected at one time under the two N environments. These QTLs for GNPR could account for 45.47 and 62.15% of phenotypic variation under high and low N conditions respectively, and all of them had negative additive effects except for the one near to Bnlg1583 (bin9.01) on chromosome 9. These results were beneficial for understanding the genetic basis of RNPE and GNPR and carrying out maker-assisted breeding project in maize.


Liu X.-H.,China West Normal University | He S.-L.,Nanchong Institute of Agricultural science | Zheng Z.-P.,Nanchong Institute of Agricultural science | Huang Y.-B.,Sichuan Agricultural University | And 5 more authors.
African Journal of Agricultural Research | Year: 2010

Quantitative trait locus (QTL) mapping can provide useful information for breeding programs, since it allows the estimation of genomic locations and genetic effects of chromosomal regions related to the expression of quantitative traits. To realize the genetic basis of grain yield of maize (Zea mays L.), a recombinant inbred line (RIL) population and two nitrogen (N) regimes were used to detect the QTLs for grain yield in maize, as a result, a total of six QTLs associated with grain yield per year (GYPE) were identified on chromosomes 1 (one), 6 (one), 8 (two) and 9 (two), with 0 - 12.0 cm of mapping interval between QTLs and their nearest markers. The three QTLs identified under high N regime could explain 18.07% of phenotypic variance, and could increase GYPE from 3.91 - 5.40 g, due to positive additive effects. Whereas, the three QTLs located under low N regime could account for 20.96% of phenotypic variance, and due to negative additive effects, they could decrease GYPE from 3.40 to 6.68 g. These results were beneficial for realizing the genetic basis of GYPE and developing marker-assisted selection in maize breeding project. ©2010 Academic Journals.


Liu X.,China West Normal University | Zheng Z.,Nanchong Institute of Agricultural science | Tan Z.,China West Normal University | Li Z.,Nanchong Institute of Agricultural science | And 4 more authors.
African Journal of Biotechnology | Year: 2010

Nitrogen (N) deficiency will severely affect many metabolic pathways and physiological progresses during maize (Zea mays L.) growth and change of anthesis-silking interval (ASI) is one of the most serious consequences. To realize the genetic basis of ASI, a recombinant inbred line (RIL) population consisting of 239 RILs, derived from the cross between Mo17 and Huangzao4, was used to identify the quantitative trait loci (QTLs) controlling ASI under different N environments. As a result, 6 QTLs were detected under high N environment on chromosome 3, 6, 7 and 8 and could explain total 53.67% of phenotypic variance. While, under low N environment, only 3 QTLs were identified on chromosome 6, 7 and 8, and they could account for total 31.87% of phenotypic variance. The two QTLs Qasihn6-1 and Qasihn3-1, identified under high N environment, were quite near to their linked marker Phi077 and Bnlg197, respectively, with less than 1 cM of genetic distance. These results are beneficial for understanding the genetic basis of ASI in maize. © 2010 Academic Journals.


Liu X.,China West Normal University | Liu X.,Key Laboratory of Southwest China Wildlife Resources Conservation Ministry of Education | He S.,Nanchong Institute of Agricultural science | Zheng Z.,Nanchong Institute of Agricultural science | And 2 more authors.
African Journal of Biotechnology | Year: 2010

Grain rate (GR) is a very important trait in maize (Zea mays L.) breeding program related to yield. To realize its genetic basis, a recombinant inbred line (RIL) population and different nitrogen (N) regimes were used to map the quantitative trait loci (QTLs) for GR in maize. As a result, two QTLs were identified under high N regime and could explain a total of 14.84% of phenotypic variance. Due to additive effect, the QTL on chromosome 6 could decrease 0.029 of GR, while the QTL on chromosome 9 could increase 0.0203 of GR. Under low N regime, one QTL was mapped on chromosome 6 and could account for 9.52% of phenotypic variance, and owning to additive effect, the QTL could make GR decrease by 0.0234. The result in comparison with previous studies showed that the three QTLs in this present study were new quantitative loci associated with GR in maize. These results were beneficial for understanding the genetic basis of GR in maize. © 2010 Academic Journals.


Liu X.-H.,China West Normal University | Zheng Z.-P.,Nanchong Institute of Agricultural science | Tan Z.-B.,Beijing IPE Bio technology Co. | Li Z.,Nanchong Institute of Agricultural science | He C.,Nanchong Institute of Agricultural science
African Journal of Biotechnology | Year: 2010

100-kernel weight (KW) is one of the most important agronomic traits in maize (Zea mays L.), related to yield. To realize its genetic basis, in this study, a recombinant inbred line (RIL) population derived from the cross between Mo17 and Huangzao4 was used for quantitative trait locus (QTL) mapping for KW under high and low nitrogen (N) regimes. As a result, five QTLs were identified on chromosomes 3, 4, 7 and 9, of which three were detected under both N environments, while the other two QTLs were respectively detected under high and low N regimes. These QTLs could explain phenotypic variance from 4.47 to 14.47%. Due to additive effects, the three QTLs from Mo17, including two on chromosome 3 and one on chromosome 4, could increase KW from 0.64 to 1.01 g, while the other two from Huangzao4 on chromosomes 7 and 9 could decrease KW from 0.62 to 1.07 g. These results are beneficial for understanding the genetic basis of KW and developing the markers linked with KW for marker-assisted selection breeding in maize. © 2010 Academic Journals.


Liu X.H.,China West Normal University | He S.L.,Nanchong Institute of Agricultural science | Zheng Z.P.,Nanchong Institute of Agricultural science | Tan Z.B.,Beijing IPE Bio Technology Co. | And 2 more authors.
Genetics and Molecular Research | Year: 2011

Kernel number per ear (KNE) is one of the most important yield-related agronomic traits in maize (Zea mays). To clarify its genetic basis, we made a quantitative trait locus (QTL) analysis of KNE in a recombinant inbred line population derived from lines Mo17 and Huangzao4, under two nitrogen (N) regimes. Seven QTLs, on chromosomes 4, 6 and 9, were mapped under the high N regime, which explained phenotypic variation ranging from 5.03 to 15.49%. Under the low N regime, three QTLs were located on chromosomes 6 and 9, which accounted for phenotypic variation ranging from 8.54 to 12.21%. These QTLs had different mapping intervals to their nearest markers, ranging from 0 to 16.5 cM. According to the chromosome positions and genetic effects of these QTLs, only seven QTLs for KNE were identified in our experiment, out of which three were found under both N regimes, on chromosomes 6 (one) and 9 (two); the other four were mapped only under the high N regime, on chromosomes 4 (three) and 6 (one). This information could be useful for developing marker-assisted selection in maize-breeding projects. © FUNPEC-RP www.funpecrp.com.br.


Zhang H.,Shanxi Agricultural University | Zhang H.,Shanxi Academy of Agricultural Sciences | Zheng Z.,Nanchong Institute of Agricultural science | Liu X.,China West Normal University | And 7 more authors.
African Journal of Agricultural Research | Year: 2010

Ear length (EL) and ear diameter (ED) are two of the most important traits in maize (Zea mays L.), related to yield and plant morphology. In this study, an F9 recombinant inbred line (RIL) population was used to identify the quantitative trait loci (QTLs) controlling EL and ED under two nitrogen (N) regimes. As a result, 3 QTLs were detected under N rich environment, 1 for EL on chromosome 1 and 2 for ED on chromosome 9. Under N stress environment, 4 QTLs were identified, 1 for EL on chromosome 1 and 3 for ED on chromosome 4 and 9. The phenotypic variances explained by these QTLs ranged from 5.68% to 9.86%, their additive effects were from -0.74 to 0.16. These results were beneficial for realizing the genetic basis of EL and ED and carrying out marker-assisted selection in maize. © 2010 Academic Journals.


PubMed | Nanchong Institute of Agricultural science
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2012

Days to silking (DTS) is one of the most important traits in maize (Zea mays). To investigate its genetic basis, a recombinant inbred line population was subjected to high and low nitrogen (N) regimes to detect quantitative trait loci (QTLs) associated with DTS. Three QTLs were identified under the high N regime; these explained 25.4% of the phenotypic variance. Due to additive effects, the QTL on chromosome 6 increased DTS up to 0.66 days; while the other two QTLs mapped on chromosome 9 (one linked with Phi061 and the other linked with Nc134) decreased DTS 0.89 and 0.91 days, respectively. Under low N regime, two QTLs were mapped on chromosomes 6 and 9, which accounted for 25.9% of the phenotypic variance. Owing to additive effects, the QTL on chromosome 6 increased DTS 0.67 days, while the other QTL on chromosome 9 decreased it 1.48 days. The QTL on chromosome 6, flanked by microsatellite markers Bnlg1600 and Phi077, was detected under both N regimes. In conclusion, we identified four QTLs, one on chromosome 6 and three on chromosome 9. These results contribute to our understanding of the genetic basis of DTS and will be useful for developing marker-assisted selection in maize breeding programs.

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