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Yu Y.,Nanchang University | Yu Y.,Nanchang Key Laboratory of Diabetes | Cai W.,Nanchang University | Pei C.-G.,Nanchang University | Shao Y.,Nanchang University
Biochemical and Biophysical Research Communications

Abstract Anti-angiogenesis targeting vascular endothelial growth factor receptor 2 (VEGFR2) has emerged as an important tool for cancer therapy. The identification of new drugs from natural products has a long and successful history. In this study, we described a novel VEGFR2 inhibitor, rhamnazin, which inhibits tumor angiogenesis and growth. Rhamnazin significantly inhibited proliferation, migration and tube formation of human umbilical vascular endothelial cells (HUVECs) in vitro as well as inhibited sprouts formation of rat aorta ring. In addition, it inhibited vascular endothelial growth factor (VEGF)-induced phosphorylation of VEGFR2 and its downstream signaling regulator in HUVECs. Moreover, rhamnazin could directly inhibit proliferation of breast cancer cells MDA-MB-231 in vitro and in vivo. Oral administration of rhamnazin at a dose of 200 mg/kg/day could markedly inhibited human tumor xenograft growth and decreased microvessel densities (MVD) in tumor sections. Taken together, these preclinical evaluations suggest that rhamnazin inhibits angiogenesis and may be a promising anticancer drug candidate. © 2015 Elsevier Inc. All rights reserved. Source

Shao Y.,Nanchang University | Yu Y.,Nanchang University | Yu Y.,Nanchang Key Laboratory of Diabetes | Li C.,Xiamen University | And 3 more authors.
RSC Advances

Ginger and quercetin have been reported to have significant antidiabetic effects. It has been proposed that 6-gingerol and quercetin are responsible for the antidiabetic activity and that a combination of the two produces a synergistic effect. However, the per se effect of 6-gingerol has not been reported. The objective of this study was to investigate the effects of 6-gingerol and quercetin per se, on streptozotocin induced non-insulin dependent diabetes mellitus (NIDDM) and hyperlipidemia. 6-Gingerol and quercetin reduced fasting glucose significantly with producing significant changes in the insulin levels of diabetic rats. The amelioration was significantly more with the combination, compared with either compound alone. The results of the oral glucose tolerance test (OGTT) indicated that 6-gingerol and quercetin reduced streptozotocin (STZ) induced increases in both area under curve glucose (AUCglucose) and area under curve insulin (AUCinsulin) in high fat feed type 2 diabetic rats. Treatment with 6-gingerol and quercetin reduced the levels of lipids in rats with NIDDM significantly. 6-Gingerol and quercetin had antihyperlipidemic effects in polaxamer P-407-induced hyperlipidemia. Quercetin and 6-gingerol show reduced serotonin induced hyperglycemia. This is the first study to demonstrate synergism of 6-gingerol and quercetin that has significant antidiabetic and beneficial cardiac effects in type 2 diabetic rats and polaxamer-induced hyperlipidemic rats. The synergistic action is probably through modulation of the serotonergic system. © The Royal Society of Chemistry 2016. Source

Yu Y.,Nanchang University | Yu Y.,Nanchang Key Laboratory of Diabetes | Yang L.,Nanchang University | Lv J.,Nanchang University | And 4 more authors.
International Journal of Clinical and Experimental Pathology

Diabetic Retinopathy (DR) is one of the most common complications of the late phase diabetes, and also a common cause of blindness. High mobility group box 1 (HMGB-1) is considered to be an inflammatory mediator in the late phase that promotes inflammation and neovascularization in diabetes. Therefore, this paper discussed the role of HMGB-1 in diabetic retinopathy inflammation and neovascularization. 96 adult SD rats were randomly divided into control and diabetes group. The diabetic rat model was established by intraperitoneal injection of streptomycin (0.1mol/L). Western blot was applied to determine HMGB-1 and its receptor RAGE and TLR2 protein expression in the serum. TUNEL was used to detect retinal apoptosis. Immunofluorescence was performed to test HMGB1 protein expression in retina. HBGM-1 and RAGE expression in diabetic rat retina was significantly higher than the control (P<0.05), while TLR2 expression was lower (P<0.05). TUNEL detection showed that diabetic rat retinal cells presented obviously higher apoptosis rate (P<0.05). Immunofluorescence test revealed that HMGB1 largely expressed in the diabetic rat retinal cells (P<0.05). HMGB1 may involve in the pathogenesis of diabetic retinopathy by binding with RAGE receptor to accelerate rat retinal cells apoptosis. Source

Zong Y.,Nanchang Key Laboratory of Diabetes | Duan P.,Nanchang Key Laboratory of Diabetes
Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]

OBJECTIVE: To investigate the impact of self-efficacy theory health education in patients of diabetic osteoporosis.METHODS: We used SPSS 19.0 software to generate random numbers and 260 diabetic osteoporosis patients were randomly divided into an observation group and a control group. There was 130 patients in observation group, 130 in control group. The self-efficacy theory health education was carried out in observation group, and routine health education in control group. SF-36 questionnaire was used to assess the quality of life. Osteoporosis self-efficacy scale (OSES) was used to assess self-efficacy. Bone mineral density (BMD) was measured by dual energy X-ray absorptiometry. Blood glucose was detected by glucose oxidase method. Glycosylated hemoglobin was detected by high efficiency liquid chromatography to estimate the index change before intervention and after one year's follow up.RESULTS: The number of patients that had completed follow-up was 104 in control group and 107 in observation group. The self-efficacy scores, movement efficiency scores and calcium intake efficiency scores were all higher in intervention group ((82.25 ± 13.54) , (79.26 ± 15.37) , (84.39 ± 17.09) points) than which in the control group ((71.14 ± 14.19), (63.89 ± 19.87), (75.24 ± 10.70) points) after one year's follow up, there were significant differences in two groups (t values were 6.04, 7.95, 5.77, all P values<0.05). The scores of quality of life in the dimension of general health, vitality, social function, role emotional and mental health were all higher in intervention group ((75.29 ± 14.90) , (68.61 ± 17.38) , (75.74 ± 18.50) , (71.22 ± 17.93) , (73.69 ± 14.40 ) points) than in the control group ( (44.25 ± 11.01) , (47.39 ± 18.90) , (63.54 ± 15.95), (49.04 ± 19.36), (55.15 ± 19.74) points), there were significant differences in two groups (t values were 8.45, 8.83, 6.92, 8.79, 8.05, all P values<0.05) . Fasting blood-glucose 2 hour postprandial blood glucose and glycosylated hemoglobin were all lower in intervention group ((7.29 ± 1.81) mmol/L, (8.21 ± 2.37) mmol/L, (6.59 ± 0.92) %) than in the control group ((8.53 ± 1.66) mmol/L, (9.41 ± 3.30) mmol/L, (7.66 ± 1.50) %) , there were significant differences in two groups (t values were 5.33, 4.67, 5.49, all P values<0.05). There were 1 (0.93%) fracture case in observation group and 7 (6.73%) cases in control group during one year's follow up, there are significant difference in two groups (χ(2) = 4.86, P = 0.028).CONCLUSIONS: The self-efficacy theory health education may improve the quality of life and decreased fracture risk of diabetic osteoporosis patients. Source

Duan P.,Nanchang Key Laboratory of Diabetes | Tu P.,Nanchang Key Laboratory of Diabetes | Wu H.,Nanchang Key Laboratory of Diabetes | Ding X.,Nanchang Key Laboratory of Diabetes | And 2 more authors.
Archives of Biological Sciences

We investigated changes in bone mineral density (BMD) and relevant factors of BMD in patients with type 1 diabetes (T1D). A total of 47 patients with T1D and 40 healthy controls participated in this study. The waist-to-hip ratio (WHR) and body mass index (BMI) were calculated after physical examination. The lumbar spine (L2-L4) BMD and left femoral neck BMD were examined. Blood samples were collected. The BMI, WHR, fasting C peptide (FCP), postprandial C peptide (2hCP), lumbar spine and left femoral neck BMD of the patients with T1D were significantly lower than those of healthy controls, while the fasting plasma glucose (FPG), postprandial plasma glucose (2hPG) and hemoglobin A1c (HbA1c) were higher (P <0.05). Duration of T1D and HbA1c were negatively correlated with lumbar spine and left femoral neck BMD. The FCP and 2hCP were positively correlated with lumbar spine and left femoral neck BMD. Source

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