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Liu J.-X.,Harbin Institute of Technology | Liu J.-X.,Qufu Normal University | Xu Y.,Harbin Institute of Technology | Zheng C.-H.,Anhui University | And 2 more authors.
IEEE/ACM Transactions on Computational Biology and Bioinformatics | Year: 2015

Microarray techniques have been used to delineate cancer groups or to identify candidate genes for cancer prognosis. As such problems can be viewed as classification ones, various classification methods have been applied to analyze or interpret gene expression data. In this paper, we propose a novel method based on robust principal component analysis (RPCA) to classify tumor samples of gene expression data. Firstly, RPCA is utilized to highlight the characteristic genes associated with a special biological process. Then, RPCA and RPCA+LDA (robust principal component analysis and linear discriminant analysis) are used to identify the features. Finally, support vector machine (SVM) is applied to classify the tumor samples of gene expression data based on the identified features. Experiments on seven data sets demonstrate that our methods are effective and feasible for tumor classification. © 2015 IEEE.

Kong H.,Nan Shan District Peoples Hospital | Tao L.,Nan Shan District Peoples Hospital | Qi K.,Nan Shan District Peoples Hospital | Wang Y.,Nan Shan District Peoples Hospital | And 3 more authors.
Oncology Reports | Year: 2013

The present study was conducted to explore the efficacy of suicide gene therapy with thymidine kinase (TK) in combination with cytosine deaminase (CD) for breast cancer. The expression of CD/TK was detected in the infected cells by RT-PCR. The killing effect on MCF-7 cells following treatment was analyzed by MTT assay. The morphological characteristics of the cells were observed by electron microscopy, and the distribution of the cell cycle was analyzed by flow cytometry. Caspase-3 and -8 activities were detected by absorption spectrometry. Cytotoxic assays showed that cells transfected with CD/TK became more sensitive to the prodrugs. Morphological features characteristic of apoptosis were noted in the MCF-7 cells via electron microscopy. The experimental data showed that the proportion of MCF-7 cells during the different phases of the cell cycle varied significantly following treatment with the prodrugs. The activity of caspase-3 gradually increased following treatment with increasing concentrations of the prodrugs. We conclude that the TK/ganciclovir and CD/5-fluorocytosine suicide gene system used here induces apoptosis in breast cancer cells, and provides a promising treatment modality for breast cancer.

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