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Kongju, South Korea

Ahn J.-H.,Namyang Dairy Co. | Kim Y.-P.,Korea Advanced Institute of Science and Technology | Kim Y.-P.,Hanyang University | Kim H.-S.,Korea Advanced Institute of Science and Technology
Food Control | Year: 2012

Lipid oxidation was effectively prevented by natural antioxidants (NA) for surface free and encapsulated oils in microencapsulated seed oil (MESO) coated with dextrin and milk protein. Three kinds of NA were extracted from rosemary, broccoli sprout, and citrus, respectively. The antioxidant effect was significantly enhanced by the use of a mixture composed of 0.05% (w/w, oil based) rosemary, 1% broccoli sprout, and 1% citrus extract. The peroxide value (POV) of the total oil in the MESO was shown to lower to about 60 meq/kg even under the accelerated storage condition at 60 ± 1 °C for 30 days. The POV of total oil from MESO was 28.1 meq/kg in the presence of antioxidants, whereas the POV of the control sample without antioxidants reached 78.8 meq/kg. The present approach can find widespread use for preventing various microencapsulated seed oils from lipid oxidation. © 2011 Elsevier Ltd.

Ahn J.-H.,Namyang Dairy Co. | Jeong Y.-S.,KAIST | Lee T.G.,Korea Research Institute of Standards and Science | Kim Y.-P.,Hanyang University | Kim H.-S.,KAIST
Biochip Journal | Year: 2012

A rapid and reliable analysis of toxins is prerequisite for food control and human healthcare. Here we demonstrate a simple and multiplexed assay of aflatoxins using time-of-flight secondary ion mass spectrometry (TOF-SIMS). By simply adsorbing either a single analyte or mixed ones onto a gold substrate, the corresponding secondary molecular ions([M+H]+) were clearly observed in a single mass spectrum. As a result of concentration-dependent peak intensity, quantitative and multiplexed analysis of different aflatoxin analogs from corns was accomplished with immunoaffinity column and TOF-SIMS analysis, which showed a good correlation with HPLC data. The detection sensitivity was estimated to be as low as 10 ng mL-1. This approach presented here will find a wide application to detection of low-levels of toxins in a rapid and multiplexed way. © 2012 The Korean BioChip Society and Springer-Verlag Berlin Heidelberg.

Khan N.,Chosun University | Jeong I.S.,Chosun University | Hwang I.M.,Chosun University | Kim J.S.,Chosun University | And 7 more authors.
Food Chemistry | Year: 2013

This study aimed to validate the analytical method for simultaneous determination of chromium (Cr), molybdenum (Mo), and selenium (Se) in infant formulas available in South Korea. Various digestion methods of dry-ashing, wet-digestion and microwave were evaluated for samples preparation and both inductively coupled plasma optical emission spectrometry (ICP-OES) and inductively coupled plasma mass spectrometry (ICP-MS) were compared for analysis. The analytical techniques were validated by detection limits, precision, accuracy and recovery experiments. Results showed that wet-digestion and microwave methods were giving satisfactory results for sample preparation, while ICP-MS was found more sensitive and effective technique than ICP-OES. The recovery (%) of Se, Mo and Cr by ICP-OES were 40.9, 109.4 and 0, compared to 99.1, 98.7 and 98.4, respectively by ICP-MS. The contents of Cr, Mo and Se in infant formulas by ICP-MS were found in good nutritional values in accordance to nutrient standards for infant formulas CODEX values. © 2013 Elsevier Ltd. All rights reserved.

Cheon S.,Sookmyung Womens University | Lee J.H.,Konkuk University | Park S.,Sookmyung Womens University | Bang S.I.,Sungkyunkwan University | And 12 more authors.
Journal of Biological Chemistry | Year: 2011

IL-32 was recently identified as a proinflammatory cytokine that is induced by IL-18 in natural killer (NK) cells and is highly correlated with inflammatory disorders. However, the relationship between IL-32 and tumor progression is still unknown. In this study, we investigated whether overexpression of IL-32 affects susceptibility of chronic myeloid leukemia (CML) cells to NK cells. Interestingly, IL-32α-overexpressing CML cell lines, K562, Kcl22, and BV173, showed higher NK cell-mediated killing. Flow cytometry analysis revealed that overexpression of IL-32α induced increased expression of Fas and UL16-binding protein 2 (ULBP2) in CML cells. The direct relationship between overexpression of surface molecules by IL-32α and increased NK cell-mediated killing was confirmed by Fas or ULBP2 siRNA transfection. IL-32α-induced Fas and ULBP2 expression are regulated p38 MAPK. In addition, the transcription factor Ets1 plays a key role in ULBP2 specific expression by IL-32α overexpression in ULBP family members. Taken together, these data show that IL-32α stimulates Fas and ULBP2 expression via activation of p38 MAPK, which increases NK susceptibility of CML cells. Enhanced NK cell susceptibility of CML cells by IL-32α overexpression may improve the efficiency of NK cell-based immunotherapy. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

Kim H.J.,Namyang Dairy Co. | Lee J.E.,Namyang Dairy Co. | Kwak B.-M.,Namyang Dairy Co. | Ahn J.-H.,Namyang Dairy Co. | Jeong S.-H.,Namyang Dairy Co.
Journal of Food Safety | Year: 2010

The contamination level of aflatoxin M1 (AFM1) in raw milk samples produced in South Korea during the winter season was investigated using immunoaffinity column chromatography and high performance liquid chromatography equipped with a fluorescence detector. Among the test samples collected from 100 cattle ranches in 2008 and 2009, none were contaminated with AFM1 over the maximum level (0.5 μg/L) set by South Korea and U.S. regulatory agencies. Seven percent of the tested samples obtained in 2008 were not contaminated with AFM1; however, this increased to 34% for samples obtained in 2009. The percent of samples with an AFM1 concentration in the range of 0.05-0.10 μg/L decreased from 21% in 2008 to 4% in 2009. In this study, the level of AFM1 in raw milk was determined to be very low and safe for market milk process. © 2010 Wiley Periodicals, Inc.

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