Nakdonggang National Institute of Biological Resources

South Korea

Nakdonggang National Institute of Biological Resources

South Korea
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Kim D.-Y.,International Ginseng & Herb Research Institute | Yeom S.-J.,Synthetic Biology & Bioengineering Research Center | Park C.-S.,Catholic University of Daegu | Kim Y.-S.,Nakdonggang National Institute of Biological Resources
Biotechnology Letters | Year: 2016

Objectives: To optimize conversion of rutin to isoquercetin by commercial α-l-rhamnosidase using high hydrostatic pressure (HHP). Results: The de-rhamnosylation activity of α-l-rhamnosidase for isoquercetin production was maximal at pH 6.0 and 50 °C using HHP (150 MPa). The enzyme showed high specificity for rutin. The specific activity for rutin at HHP was 1.5-fold higher than that at atmospheric pressure. The enzyme completely hydrolysed 20 mM rutin in tartary buckwheat extract after 2 h at HHP, with a productivity of 10 mM h−1. The productivity and conversion were 2.2- and 1.5-fold higher at HHP than at atmospheric pressure, respectively. Conclusions: This is the first report concerning the enzymatic hydrolysis of isoquercetin in tartary buckwheat at HHP. © 2016 Springer Science+Business Media Dordrecht

PubMed | Soonchunhyang University, GnC BIO Co., Chonnam National University, Korea Research Institute of Bioscience and Biotechnology and 4 more.
Type: Journal Article | Journal: Genes | Year: 2016

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PubMed | Kyungpook National University, Daegu Haany University, Gachon University, Korea Research Institute of Bioscience and Biotechnology and Nakdonggang National Institute of Biological Resources
Type: Journal Article | Journal: Journal of medicinal food | Year: 2016

The traditional manufacturing method used to produce goods from Hizikia fusiforme, utilizes extraction steps with hot water. The byproduct (of hot water extraction) is rich in polysaccharide and is considered a waste. To evaluate the osteogenic effects of the byproduct of H. fusiforme (HFB), osteogenic cells and animal models were used to test it effects on osteogenesis. The HFB-treated mouse myoblast C2C12 cells exhibited significant dose dependently elevated alkaline phosphatase (ALP) activity and slightly increased bone morphogenetic protein-2 (BMP-2). HFB also suppressed the formation of tartrate-resistant acid phosphatase (TRAP) activity and TRAP staining in the bone marrow-derived macrophages (BMM) cells that had been stimulated with the receptor activator of the nuclear factor kB ligand/macrophage colony-stimulating factor kB ligand. In addition, HFB also increased the phosphorylation of extracellular signal-regulated protein kinase (p-ERK) level. Finally, osteogenic effects of HFB were clearly confirmed in the three in vivo models: zebrafish, ovariectomized mice, and mouse calvarial bones. HFB accelerated the rate of skeletal development in zebrafish and prevented much of the mouse femoral bone density loss of ovariectomized mice. Moreover, HFB enhanced woven bone formation over the periosteum of mouse calvarial bones. Our result showed that HFB functions as a bone resorption inhibitor as well as an activator of bone formation in vivo and in osteogenic in vitro cell systems.

PubMed | Jeju National University, Jeju Biodiversity Research Institute, Hanbul Cosmetics Co. and Nakdonggang National Institute of Biological Resources
Type: | Journal: EXCLI journal | Year: 2015

During our on-going screening program designed to isolate natural compounds from marine environments, we isolated isoketochabrolic acid (IKCA) from Sargassum micracanthum, an important brown algae distributed in Jeju Island, Korea. Furthermore, we evaluated the inhibitory effects of IKCA on nitric oxide (NO) production in lipopolysaccharide (LPS)-triggered macrophages. IKCA strongly inhibited NO production, with an IC50 value of 58.31 M. Subsequent studies demonstrated that IKCA potently and concentration-dependently reduced prostaglandin E2 (PGE2), tumor necrosis factor-alpha (TNF-), interleukin (IL)-1, and IL-6 cytokine production. In conclusion, to the best of our knowledge, this is the first study to show that IKCA isolated from S. micracanthum has a potent anti-inflammatory activity. Therefore, IKCA might be useful as an anti-inflammatory health supplement or functional cosmetics.

PubMed | Chungnam National University, Korea Atomic Energy Research Institute and Nakdonggang National Institute of Biological Resources
Type: Journal Article | Journal: Molecules (Basel, Switzerland) | Year: 2016

In this study, we investigated the anti-inflammatory effect of rosmarinic acid methyl ester (RAME) isolated from a mutant cultivar of Perilla frutescens (L.) Britton. We found that RAME inhibits lipopolysaccharide (LPS)-induced nitric oxide (NO) production, with an IC50 of 14.25 M, in RAW 264.7 cells. RAME inhibited the LPS-induced expression of pro-inflammatory cytokines including interleukin (IL)-1, IL-6, IL-10, monocyte chemoattractant protein-1, interferon-, and inducible nitric oxide synthase (iNOS). Moreover, RAME suppressed the activation of nuclear factor kappa B. These results suggest that the downregulation of iNOS expression by RAME was due to myeloid differentiation primary response gene 88 (MyD88)-dependent and -independent pathways. Furthermore, RAME induced the expression of heme oxygenase-1 (HO-1) through activation of nuclear factor-erythroid 2-related factor 2. Treatment with tin protoporphyrin, an inhibitor of HO-1, reversed the RAME-induced suppression of NO production. Taken together, RAME isolated from P. frutescens inhibited NO production in LPS-treated RAW 264.7 cells through simultaneous induction of HO-1 and inhibition of MyD88-dependent and -independent pathways.

PubMed | 8Korea Research Institute of Bioscience & Biotechnology KRIBB, 4Korea Research Institute of Bioscience & Biotechnology KRIBB, Korea Research Institute of Bioscience and Biotechnology, 1Nanjing Forestry University and 2 more.
Type: | Journal: International journal of systematic and evolutionary microbiology | Year: 2016

A polyphasic taxonomic study was carried out on strain EBR-4-2T isolated from a biofilm reactor in Korea. The cells of the strain were Gram-staining-positive, non-spore-forming, non-motile, and rod-shaped. Comparative 16S rRNA gene sequence studies showed a clear affiliation of this strain with Actinobacteria, which had the highest pairwise sequence similarities with Actinotalea suaedae EGI 60002T (98.7 %), Actinotalea ferrariae CF5-4T (96.3 %) and Actinotalea fermentans DSM 3133T (96.2 %). The phylogenetic analysis based on the 16S rRNA gene sequences showed that the strain formed a clear phylogenetic lineage with the genus Actinotalea. The major fatty acids were identified as C15:0 anteiso, C16:0, C16:0 N alcohol, C15:1 anteiso A and C15:0 iso. The major cellular polar lipids were identified as diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol and glycolipid. The peptidoglycan type was A4 containing L-Orn-D-Glu. The whole-cell-wall sugars were glucose and ribose. The respiratory quinone was identified as MK-10(H4), and the genomic DNA G+C content was determined to be 74.8 mol%. Based on the polyphasic evidence, it is proposed that strain EBR-4-2T should be designated as a new species named Actinotalea caeni sp. nov. The type stain is EBR-4-2T (=KCTC 33604T =JCM 30447T).

Song M.-Y.,Inland Fisheries Research Institute | Lee W.-O.,Inland Fisheries Research Institute | Kim K.-H.,Inland Fisheries Research Institute | Park Y.-S.,Kyung Hee University | Bae M.-J.,Nakdonggang National Institute of Biological Resources
Annales de Limnologie | Year: 2016

Dams can cause fragmentation of the natural structure and function of fish communities up- and downstream of the dam. In this study, we examined the effects of the Goesan Dam on the longitudinal distribution of fish in the Dal stream, using two computational methods: a self-organizing map (SOM) and spatial autocorrelation. Fish were collected at 16 different study sites in two seasons (spring and autumn). SOM classified the study sites into four clusters (1-4), reflecting differences in the longitudinal distribution patterns of fish, and the effects of the dam on fish community composition. Samples in clusters 1 and 2 mostly originated from tributaries and the upstream section of the dam, whereas samples in cluster 4 originated from downstream study sites of the dam. Cluster 3 included study sites originating directly from up- and downstream of the dam. Species turnover and spatial autocorrelation results confirmed the fish community alteration near the dam. Species turnover was the highest between study sites directly up- and downstream of the dam. The Mantel correlogram was positive when applied to short distances between the study sites. However, it was negative for the farthest distance, including sites near the dam, indicating that the dam caused significant alterations to the fish community. Overall, our results suggest that the habitat alterations (e.g., fragmentation of longitudinal gradients and disruption of flow regimes) due to dam construction have caused fragmentation of fish communities. © 2016 EDP Sciences.

Jeong H.I.,Chung - Ang University | Jin H.M.,Chung - Ang University | Jin H.M.,Nakdonggang National Institute of Biological Resources | Jeon C.O.,Chung - Ang University
Journal of Biotechnology | Year: 2016

Sphingorhabdus sp. M41, capable of degrading aliphatic and aromatic hydrocarbons, was isolated from crude oil-contaminated costal sediment by an enrichment culture and its complete genome was sequenced. The genome of strain M41 has a chromosome with a size of 3,324,420 bp, including 44 tRNAs, 6 rRNAs, and 3118 protein-coding genes. In addition, many potential genes responsible for the biodegradation of aliphatic and aromatic hydrocarbons were identified from the genome. This is the first complete genome of the genus Sphingorhabdus, which will provide insights into the bioremediation of crude oil-contaminated costal sediment by strain M41. © 2016.

Kim K.H.,Chung - Ang University | Jin H.M.,Chung - Ang University | Jin H.M.,Nakdonggang National Institute of Biological Resources | Jeong H.I.,Chung - Ang University | Jeon C.O.,Chung - Ang University
International Journal of Systematic and Evolutionary Microbiology | Year: 2016

A Gram-staining-negative, moderately halophilic and strictly aerobic bacterium, designated strain KJ4T, was isolated from marine sediment at Gangjin in South Korea. Cells were catalaseand oxidase-positive long rods with gliding motility. Growth of strain KJ4T was observed at 4-37 °C (optimum, 15-25 °C), at pH 6.0-9.0 (optimum, pH 7.0-8.5) and in the presence of 1.0-7.0 % (w/v) NaCl (optimum, 2.0-5.0 %). Menaquinone 6 (MK-6) was the only isoprenoid quinone detected and iso-C15: 0, iso-C17: 0 3-OH, iso-C15: 1 G and summed feature 3 (comprising C16: 1ω7c and/or C16: 1ω6c) were the major cellular fatty acids. The polar lipids of strain KJ4T consisted of phosphatidylethanolamine, an unidentified aminophospholipid and five unidentified lipids. The G+C content of the genomic DNA was 38.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KJ4T formed a distinct phylogenetic lineage within the genus Maribacter. Strain KJ4T was most closely related to Maribacter orientalis KMM 3947T with 97.4 % 16S rRNA gene sequence similarity. On the basis of phenotypic, chemotaxonomic and molecular features, strain KJ4T clearly represents a novel species of the genus Maribacter, for which the name Maribacter lutimaris sp. Nov. Is proposed. The type strain is KJ4T (=KACC 16438T=JCM 31154T). © 2016 IUMS.

Yun S.M.,Sangmyung University | Lee S.D.,Nakdonggang National Institute of Biological Resources | Park J.S.,Sangmyung University | Lee J.H.,Sangmyung University
Algae | Year: 2016

Paralia species have been frequently reported as P. sulcata in Korea, despite the species diversity within the genus. To understand the species diversity of Paralia in Korea, we collected phytoplankton samples at 79 sites from April 2006 to April 2015. Five Paralia species, P. fenestrata, P. guyana, P. marina, P. cf. obscura, and P. sulcata, were observed during this study, and we described their fine structure in terms of quantitative and qualitative morphological characteristics. To provide additional criteria to identify Paralia species more clearly, we morphometrically analysed four quantitative characteristics on valve diameter: pervalvar axis / diameter, internal linking spines / diameter, marginal linking spines/diameter, and fenestrae/diameter using non-metric multidimensional scaling (MDS). MDS analysis distinguished four Paralia species: P. guyana, P. marina, P. cf. obscura, and P. sulcata, with the exception of P. fenestrata. This new approach in using morphometric analysis is useful for the accurate identification of Paralia species. © 2016 The Korean Society of Phycology.

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