Nakano Seiyaku Co.

Kyoto, Japan

Nakano Seiyaku Co.

Kyoto, Japan

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Washika S.,Gunma University | Yoshii N.,Nakano Seiyaku Co. | Fukuta S.,Nakano Seiyaku Co. | Nishiyama S.,Nakano Seiyaku Co. | And 4 more authors.
Sen'i Gakkaishi | Year: 2012

Two types of peptide fractions were extracted from the human hairs and the properties for the fractions were investigated. The molecular weight of A fractions was 11 kDa, and that of B fractions 9.7 kDa. Artificially damaged human hairs were prepared by repeating the bleach treatments, and the recoveries in physical properties for the damaged hairs were estimated by impregnating those extracted peptide fractions. When the A fractions were impregnated into the damaged hairs, the formation of permanent waves was slightly recovered. As for the B fractions the tensile strengths for the damaged hairs were regained to some extent.


Horibe I.,Nakano Seiyaku Co. | Satoh Y.,Kansai University | Shiota Y.,Kansai University | Kumagai A.,Kansai University | And 9 more authors.
Journal of Natural Medicines | Year: 2013

Agents to control melanogenesis are in demand for the development of cosmetics to improve pigmentation disorders of skin and hair. In this study, we examined and evaluated the effects of flavonoids on melanogenesis in the melanogenic cells model, murine B16F10 melanoma cells. In the course of this study, we found that incubation of the cells in a medium containing 10 μM of the 4′-O-methylated flavonoids, diosmetin (4′-O-methylluteolin), acacetin (4′-O-methylapigenin) or kaempferide (4′-O- methylkaempferol), increased the melanin contents of the cells 3- to 7-fold higher than the control cells. The concentration-dependence test revealed that 20 μM acacetin showed the highest effect, up to 33-fold higher than the vehicle. On the other hand, the corresponding 4′-OH-type flavonoids, luteolin, apigenin and kaempferol, had a significantly smaller effect. Furthermore, by evaluating the melanogenic proteins, we found that the cells treated with 4′-O-methylated flavonoids showed higher tyrosinase activity, as well as upregulation of tyrosinase expression, preceded by activation of cAMP response element binding protein (CREB) and extracellular signal-regulated kinases types 1 and 2 (ERK1/2). These results indicate that the 4′-O-methyl group of flavonoids plays an important role in the induction of melanogenesis by activating its major signal transduction pathway through the upregulation of phospho-CREB in murine B16F10 melanoma cells. © 2012 The Japanese Society of Pharmacognosy and Springer Japan.

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