Time filter

Source Type

Kashiwa, Japan

Takahashi I.,Nakano Ladies Clinic | Hatori A.,Nakano Ladies Clinic | Nakano H.,Nakano Ladies Clinic
Journal of Mammalian Ova Research | Year: 2013

We examined whether partial thinning of zona pellucida by a LAH (laser-assisted hatching) system in ICSI could ease pressure on the egg and influence the results of subsequent embryogenesis and pregnancy rate (90 subjects, 101 cycles). ICSI was performed with and without thinning of zona pellucida in a thinning group and a control group, respectively, and the results of embryogenesis and pregnancy rate were compared between the two groups. Fertilization rate, oocyte degeneration rate, good embryo rate, and pregnancy rate in early embryo implantation were higher, though not significantly, in the thinning group (80.8%, 7.5%, 66.5%, and 28.6%) than in the control group (73.0%, 9.7%, 64.6%, and 23.1%). The fertilization rate was significantly higher in the thinning group compared to the control group. Our results suggest that partial thinning of zona pellucida using a LAH system is useful in ICSI. Source

Hatori A.,Nakano Ladies Clinic | Takahashi I.,Nakano Ladies Clinic | Nakano H.,Nakano Ladies Clinic
Journal of Mammalian Ova Research | Year: 2011

We investigated how the visibility and position of the mitotic spindle which had undergone affects embryogenesis. The subjects were 830 oocytes at metaphase II intracytoplasmic sperm injection (ICSI). When the mitotic spindle was visible, oocytes were classified into the following groups according to its position: Group A, directly beneath the polar body; Group B, at 0°< to ≤45° clockwise from the polar body; Group C, at 45°< to ≤90°; Group D, 90°< to ≤135°; and Group E, 135°< to ≤180°. When invisible, oocytes were classified into Group F. The percentage of Groups A to F were 57.4%, 38.5%, 3.5%, 0.5%, 0.1%, and 27.9%, respectively. The fertilization rate, the good embryogenesis rate, the blastocyst formation rate, and the good blastocyst formation rate of the visible oocytes (Groups A to E) were 70.6%, 48.3%, 41.5%, and 5.2%, respectively, and those of the invisible oocytes (Group F) were 65.9%, 47.7%, 29.5%, and 4.2%, respectively. The fertilization rate and the blastocyst formation rate tended to be higher for the visible oocytes. We confirmed that visualization of the mitotic spindle after ICSI is a meaningful method. Source

Discover hidden collaborations