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Fukuoka-shi, Japan

Nakamura Gakuen University is a private university in Fukuoka, Fukuoka, Japan. The predecessor of the school was founded in 1954. It was chartered as a junior college in 1957 and became a four-year college in 1965. Wikipedia.

Suwa M.,Tohoku Institute of Technology | Nakano H.,Nakamura Gakuen University | Radak Z.,Semmelweis University | Kumagai S.,Kyushu University
Metabolism: Clinical and Experimental

Adenosine monophosphate-activated protein kinase (AMPK) has been proposed to stimulate mitochondrial biogenesis and fat and glucose metabolism in skeletal muscle. Nicotinamide adenine dinucleotide-dependent histone deacetylase sirtuin 1 (SIRT1) is also thought to play a pivotal role for such metabolic adaptations. The purpose of the present study was to examine the effect of AMPK activation with the administration of AMPK activator 5-aminoimidazole-4- carboxamide-1-β-d-ribofuranoside (AICAR) to rats on skeletal muscle SIRT1 protein expression as well as peroxisome proliferator activated receptor γ coactivator-1α (PGC-1α) and glucose transporter 4 (GLUT4) protein expression and hexokinase activity. The AICAR promoted the phosphorylation of AMPK α-subunit (Thr172) and acetyl-coenzyme A carboxylase (Ser79) without any change of total AMPK α-subunit or acetyl-coenzyme A carboxylase protein levels in both the slow-twitch soleus and fast-twitch extensor digitorum longus (EDL) muscles. The SIRT1 protein expression increased at 24 hours after administration of AICAR in the EDL muscle but not in the soleus muscle. The PGC-1α protein expression increased in both the soleus and EDL muscles and GLUT4 did in the EDL muscle at 24 hours after an administration of AICAR. The hexokinase activity increased at 18 and 24 hours in the soleus and at 12, 18, and 24 hours in the EDL after an AICAR treatment. These results suggest that short-term AICAR treatment to rats promotes skeletal muscle AMPK phosphorylation and then coincidently increases the SIRT1 protein expression. In addition, such treatment also enhances the PGC-1α and GLUT4 protein contents and hexokinase activity in skeletal muscle. Crown Copyright © 2011 Published by Elsevier Inc. All rights reserved. Source

Shiikuwasha (Citrus depressa Hayata) is produced in the northern area of the main Okinawa island, the southern most region of Japan, and is a typical Japanese citrus along with Tachibana (Citrus tachibana). The seed of Shiikuwasha contains high concentration of limonoid glucosides, including limonin glucoside and nomilin glucoside, which has anti-tumor activity. Synephrine, a well-known enhancer of lipid metabolism, is also present in the fruit peel. The most notable feature of Shiikuwasha, among citrus fruits harvested commercially in Japan, is its high concentration of polymethoxylflavones, such as nobiletin (NBL), tangeretin and sinensetin. These are present in the peel, where NBL is predominant. Many studies have demonstrated that NBL possesses various biological properties such as anti-carcinogenic, anti-inflammatory and hepatoprotective activities. The ingestion of Shiikuwasha paste decreases plasma glucose level in laboratory animals, such as mouse and rat, as well as human volunteers. Study of the metabolism, by animal and human liver microsomes and cytochrome P450 of NBL shows the presence of 4'-OH, 7-OH, 6-OH metabolites, as well as the novel metabolites 3' and 4'-diOH NBL. These results indicate that Shiikuwasha is an attractive foodstuff from the perspective of health promotion. Furthermore, in order to maintain Shiikuwasha authenticity, methods distinguishing between Shiikuwasha and Calamondin were developed using a unique phloretin glucoside and certain polymethoxyflavones as chemical markers, as well as Shiikuwasha chloroplast DNA. Source

The in vitro metabolism of 2, 2', 3, 4', 5, 5'-hexachlorobiphenyl (hexaCB) (CB146) was examined using liver microsomes of rats, guinea pigs, hamsters and human. Untreated animal livers produced one metabolite (M-2) in rats, three metabolites (M-l, M-2 and M-3) in guinea pigs and no metabolite in hamsters. Pretreatment of phenobarbital (PB) resulted in a marked increase of M-1 in three animals and of M-2 in guinea pigs. In contrast, pretreatment of 3-methylcholanthrene showed a significant increase of M-3 in guinea pigs and a decrease of M-2 in rats. Human liver microsomes prepared from nine Caucasians mainly formed M-2 and M-3 at a ratio of 2 : 1 and two individuals also formed one more metabolite M-1. The formation of M-1 was significantly correlated with CYP2B6 activity. By comparison of the GC-MS data of three metabolites with synthesized authentic samples, M-1 and M-2 were determined to be 3'-hydroxy (OH)-CB146 and 4-OH-CB146, respectively. However, M-3 is unclear at present except the fact that it is OH-hexaCB. These results suggest that 3'-OH-CB146 is formed by PB-inducible cytochrome P450 (CYP2B enzymes) in animal and human livers and 4-OH-CB146 is a major metabolite in rat and human liver. Source

Hamasaki N.,Nagasaki International University | Kuma H.,Nagasaki International University | Tsuda H.,Nakamura Gakuen University
Annals of Laboratory Medicine

Thrombophilia that is common among Caucasians is caused by genetic polymorphisms of coagulation factor V Leiden (R506Q) and prothrombin G20210A. Unlike that in Caucasians, thrombophilia that is common in the Japanese and Chinese involve dysfunction of the activated protein C (APC) anticoagulant system caused by abnormal protein S and protein C molecules. Approximately 50% of Japanese and Chinese individuals who develop venous thrombosis have reduced activities of protein S. The abnormal sites causing the protein S molecule abnormalities are distributed throughout the protein S gene, PROS1. One of the most common abnormalities is protein S Tokushima (K155E), which accounts for about 30% of the protein S molecule abnormalities in the Japanese. Whether APC dysfunction occurs in other Asian countries is an important aspect of mapping thrombophilia among Asians. International surveys using an accurate assay system are needed to determine this. © The Korean Society for Laboratory Medicine. Source

Ozawa M.,Kyushu University | Ninomiya T.,Kyushu University | Ohara T.,Kyushu University | Doi Y.,Kyushu University | And 5 more authors.
American Journal of Clinical Nutrition

Background: To our knowledge, there are no previous reports that assessed the association between dietary patterns and risk of dementia in Asian populations. Objective: We investigated dietary patterns and their potential association with risk of incident dementia in a general Japanese population. Design: A total of 1006 community-dwelling Japanese subjects without dementia, aged 60-79 y, were followed up for a median of 15 y. The reduced rank regression procedure was used to efficiently determine their dietary patterns. Estimated risk conferred by a particular dietary pattern on the development of dementia was computed by using a Cox proportional hazards model. Results: Seven dietary patterns were extracted; of these, dietary pattern 1 was correlated with high intakes of soybeans and soybean products, vegetables, algae, and milk and dairy products and a low intake of rice. During the follow-up, 271 subjects developed allcause dementia. Of these individuals, 144 subjects had Alzheimer disease (AD), and 88 subjects had vascular dementia (VaD). After adjustment for potential confounders, risks of development of allcause dementia, AD, and VaD were reduced by 0.66 (95% CI: 0.46, 0.95), 0.65 (95% CI: 0.40, 1.06), and 0.45 (95% CI: 0.22, 0.91), respectively, in subjects in the highest quartile of score for dietary pattern 1 compared with subjects in the lowest quartile. Conclusion: Our findings suggest that a higher adherence to a dietary pattern characterized by a high intake of soybeans and soybean products, vegetables, algae, and milk and dairy products and a low intake of rice is associated with reduced risk of dementia in the general Japanese population. © 2013 American Society for Nutrition. Source

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