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Ibrahim A.M.A.,Assiut University | Ibrahim A.M.A.,Humanity | Alrakan L.A.,Naif Arab University for Security science | Alaifan S.A.,King Saud University
Entomological Research | Year: 2015

Female mosquitoes feed on human blood, which can be collected to analyze human short tandem repeat (STR) sequences; these are specific to each human individual. Analysis of STRs might help in identification of a person found near a crime scene. Aedes aegypti and Culex pipiens mosquitoes fed on human blood were cultured at 18°C or 40°C (median temperature for summer and winter time in Riyadh governorate, Saudi Arabia) for 3, 6, 12, 24, 48 and 72h. In A.aegypti, human DNA concentration was reduced with time at both temperatures. At 18°C, we obtained full STR profiles up to 48h post feeding on human blood while none of the 16 loci were obtained at 72h. At 40°C, we missed six sites at 12h after blood sucking, 12 at 24h, and 15 at 48h and 72h. In C.pipiens cultured at 18°C, full profiles were developed up to 48h following blood feeding while we could not amplify five sites at 72h. At 40°C, mortality among females was 50% at 24h and 100% at both 48h and 72h; however, we had full profiles in all samples including dead insects. This research addressed the possibility of using mosquitoes in forensic research by DNA genotyping by changing the mosquito culturing temperature and mosquito genus. Our findings proved that different types of mosquito change the temporal pattern of STR analysis and showed that the mosquito culturing temperature affects the integrity of DNA for STR analysis. © 2015 The Entomological Society of Korea and Wiley Publishing Asia Pty Ltd. Source

Abd-El-Nabey B.A.,Alexandria University | Abdel-Gaber A.H.,Alexandria University | Ali M.E.S.,Naif Arab University for Security science | Khamis E.,Alexandria University | El-Housseiny S.,Alexandria University
International Journal of Electrochemical Science | Year: 2013

The effect of extract of cannabis plant on the corrosion of copper in aqueous 0.5M sulphuric acid was investigated by electrochemical impedance spectroscopy (EIS), potentiodynamic polarization, weight loss and optical micrograph techniques. EIS and polarization measurements showed that the dissolution process of copper occurs under diffusion control. Potentiodynamic polarization curves indicated that the plant extract behaves as cathodic-type inhibitor. The corrosion rates of copper and the inhibition efficiencies of the extract were calculated. The results obtained show that the extract solution of the plant could serve as an effective inhibitor for the corrosion of copper in sulphuric acid media. Theoretical fitting of different isotherms, Langmuir, Flory-Huggins, and the kinetic-thermodynamic model, were tested to clarify the nature of adsorption. © 2013 by ESG. Source

Al-Okour A.,Al - Balqa Applied University | Ajlouni A.-W.,Naif Arab University for Security science | Ajlouni A.,Al - Balqa Applied University | Shakhatreh S.,Al - Balqa Applied University
Contemporary Engineering Sciences | Year: 2013

Gamma Radiation measurements conducted in Al Hammah hot springs, Jordan are introduced in this paper. These data represent new result of high-radiation dose equivalents found in part of the country measured for the first time. The radiation doses, attributable to terrestrial gamma radiation, were measured using a portable Geiger-Muller counter and an NaI(Tl) detector. The measured absorbed dose rates in air ranged from 70 to 580 nGyh-1, in hot water spring areas. The maximum high radiation dose was nearby the principal hot water source of the cooling pool. The gamma doses outside the spa region were 30 to 70 nGyh-1, which is higher than average registered radiation doses rates in Jordan. © 2013 Amin Al-Okour et al. Source

Hussein A.S.,Naif Arab University for Security science | Hussein A.S.,Research Institute of Nuclear Power Plants
Radioprotection | Year: 2014

Radon (222Rn) is a radioactive gas that occurs naturally when uranium in soil and rock breaks down. Long-term exposure to 222Rn increases the risk of lung cancer. The principal objective of this work was to determine the 222Rn activity concentration in an indoor air environment at the El-Dabaa site proposed for a nuclear power plant project in Egypt using the track etch technique with LR115 detectors. The annual average indoor 222Rn activity concentration in apartment buildings varies from 24 to 77 Bq.m-3, with a mean value of 54 Bq.m-3. The annual effective dose received by residents of the studied area was estimated to be 0.41 mSv. The annual estimated effective dose is less than the recommended action level by ICRP (3-10 mSv.y-1). The results from this work provide a radiological assessment program and update the background of the natural radioactivity map at the El-Dabaa site. © EDP Sciences, 2014. Source

Refaat A.M.,Naif Arab University for Security science
Egyptian Journal of Medical Human Genetics | Year: 2016

Background: Azoospermia constitutes 20% of male infertility situations and affects 1% of the total male population (Jarvi et al., 2010). This condition is classified into three major types; pre-testicular, testicular and post-testicular Azoospermia (Sermondade et al., 2012). Genetic defects causing Azoospermia are due to chromosomal or non-chromosomal alterations on the Y-Chromosome (Lee et al., 2011). Initial diagnosis of Azoospermia is established when no spermatozoa are detected on microscopic examination of semen (World Health Organisation, 1999). Objective: To evaluate the correlation of Y-STR Profiling results and the prevalence of Azoospermia, to help for early prognosis of Azoospermia before puberty. Methods: Buccal swab samples were taken from two groups of individuals (50 fertile and 50 Azoospermic patients), then DNA was isolated using QIAamp DNA Micro kit. DNA quantification was done using a Real-time PCR utilizing Quantifiler Kit. PCR was done using PowerPlex® Y PCR Amplification Kit, then amplified products were typed using a 3130 Genetic Analyzer. Results: Five haplotypes in four different Y-STR loci were found to possess significantly higher occurrence percentages in Azoospermic than in fertile Saudi individuals, which can serve as a group of pre-diagnostic markers for early prognosis of Azoospermia in Saudi population. Conclusion: There was a significant correlation of Y-STR Profiling results and the prevalence of Azoospermia condition, which supports the idea of using Y-STR Profiling in early prognosis of Azoospermia. © 2015 The Author. Source

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