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Ahmed Y.M.,Nahda University | Messiha B.A.S.,Beni Suef University | Abo-Saif A.A.,Nahda University
Pharmacology | Year: 2015

Background/Aims: Rheumatoid arthritis (RA) is a disabling autoimmune disease for which most current treatments cause massive complications, thereby limiting treatment dose and duration. The anti-arthritic effects of the 3-hydroxy-3-metylglutary-CoA reductase inhibitor, simvastatin, and the natural flavonoid, hesperidin, were investigated against complete Freund's adjuvant-induced RA in rats. Methods: A normal control group, an arthritis control group, 2 reference treatment groups receiving dexamethasone (1.5 mg/kg/day) and methotrexate (1 mg/kg/day), and 2 treatment groups receiving simvastatin (0.5 mg/kg/day) and hesperidin (200 mg/kg/day) were included in the study. Serum rheumatoid factor, matrix metalloprotinease-3 (MMP-3) and cartilage oligomeric matrix protein (COMP) as specific rheumatoid biomarkers, serum immunoglobulin G (IgG) and antinuclear antibody (ANA) as immunological biomarkers, serum tumor necrosis factor-alpha and interleukin-10 as immunomodulatory cytokines, serum myeloperoxidase (MPO) and C-reactive protein as inflammatory biomarkers, and malondialdehyde (MDA) and glutathione (GSH) as oxidative stress biomarkers were assessed, supported by joint and spleen histopathological study. Results: Simvastatin significantly improved all the measured biomarkers, with MMP-3, COMP, and GSH restored to normal levels. Hesperidin significantly improved all the measured biomarkers, with COMP, IgG, ANA, MPO, MDA and GSH restored to normal levels. Conclusion: Simvastatin and hesperidin may be promising protective agents against RA through immunomodulatory, anti-inflammatory and antioxidant potentials. © 2015 S. Karger AG, Basel. All rights reserved.


Fahmy Wahba M.G.,Nahda University | Shehata Messiha B.A.,Beni Suef University | Abo-Saif A.A.,Nahda University
European Journal of Pharmacology | Year: 2015

Rheumatoid arthritis (RA) is a challenging autoimmune disorder, whose treatments usually cause severe gastrointestinal, renal and other complications. We aimed to evaluate the beneficial anti-arthritic effects of an angiotensin converting enzyme (ACE) inhibitor, ramipril and a dopamine receptor blocker, haloperidol, on Complete Freund's Adjuvant-induced RA in adult female albino rats. Rats were allocated into a normal control group, an arthritis control group, two reference treatment groups receiving dexamethasone (1.5 mg/kg/day) and methotrexate (1 mg/kg/day), and two treatment groups receiving ramipril (0.9 mg/kg/day) and haloperidol (1 mg/kg/day). Serum rheumatoid factor, matrix metalloprotinease-3 (MMP-3) and cartilage oligomeric matrix protein as specific rheumatoid biomarkers, serum immunoglobulin G and antinuclear antibody as immunological biomarkers, serum tumor necrosis factor alpha (TNF-α) and interleukin-10 (IL-10) as immunomodulatory cytokines, serum myeloperoxidase and C-reactive protein as inflammatory biomarkers, as well as malondialdehyde and glutathione reduced (GSH) as oxidative stress biomarkers were assessed. A histopathological study on joints and spleens was performed to support the results of biochemical estimations. Ramipril administration significantly corrected all the measured biomarkers, being restored back to normal levels except for MMP-3, TNF-α and IL-10. Haloperidol administration restored all the measured biomarkers back to normal levels except for TNF-α, IL-10 and GSH. In conclusion, ACE inhibitors represented by ramipril and dopamine receptor blockers represented by haloperidol may represent new promising protective strategies against RA, at least owing to their immunomodulatory, anti-inflammatory and antioxidant potentials. © 2015 Elsevier B.V. All rights reserved.


Mohammed N.E.M.,Nahda University | Messiha B.A.S.,Beni Suef University | Abo-Saif A.A.,Nahda University
Saudi Pharmaceutical Journal | Year: 2016

Background Exposure to chemotherapeutic agents such as acetaminophen may lead to serious liver injury. Calcium deregulation, angiotensin II production and xanthine oxidase activity are suggested to play mechanistic roles in such injury. Objective This study evaluates the possible protective effects of the calcium channel blocker amlodipine, the angiotensin converting enzyme inhibitor lisinopril, and the xanthine oxidase inhibitor allopurinol against experimental acetaminophen-induced hepatotoxicity, aiming to understand its underlying hepatotoxic mechanisms. Material and methods Animals were allocated into a normal control group, a acetaminophen hepatotoxicity control group (receiving a single oral dose of acetaminophen; 750 mg/kg/day), and four treatment groups receive N-acetylcysteine (300 mg/kg/day; a reference standard), amlodipine (10 mg/kg/day), lisinopril (20 mg/kg/day) and allopurinol (50 mg/kg/day) orally for 14 consecutive days prior to acetaminophen administration. Evaluation of hepatotoxicity was performed by the assessment of hepatocyte integrity markers (serum transaminases), oxidative stress markers (hepatic malondialdehyde, glutathione and catalase), and inflammatory markers (hepatic myeloperoxidase and nitrate/nitrite), in addition to a histopathological study. Results Rats pre-treated with amlodipine, lisinopril or allopurinol showed significantly lower serum transaminases, significantly lower hepatic malondialdehyde, myeloperoxidase and nitrate/nitrite, as well as significantly higher hepatic glutathione and catalase levels, compared with acetaminophen control rats. Serum transaminases were normalized in the lisinopril treatment group, while hepatic myeloperoxidase was normalized in the all treatment groups. Histopathological evaluation strongly supported the results of biochemical estimations. Conclusion Amlodipine, lisinopril or allopurinol can protect against acetaminophen-induced hepatotoxicity, showing mechanistic roles of calcium channels, angiotensin converting enzyme and xanthine oxidase enzyme in the pathogenesis of hepatotoxicity induced by acetaminophen. © 2015 The Authors


Ahmed O.M.,Beni Suef University | Ahmed O.M.,Nahda University | Gabar M.A.,Beni Suef University | Ali T.M.,Beni Suef University
Journal of Diabetes and its Complications | Year: 2012

The present study was conducted to assess the interrelationship and the influence of the coexistence of diabetes and hypothyroidism on thyroid hormone levels, insulin levels and biochemical variables related to carbohydrate, lipid and protein metabolism in addition to thyroid gland and Islets of Langerhans histological changes and antioxidant defense system. Diabetes mellitus was induced by intraperitoneal injection of streptozotocin to fasting albino rats at dose level of 45 mg/kg b. w. Hypothyroidism in diabetic and normal rats was induced by adding methimazole in drinking water (0.02% w/v) for 4 weeks. The obtained results revealed that hypothyroidism interacts with diabetes in a way that prevents the progress of the hyperglycemic state. This may be due to the increase in the insulin secretory response in diabetic hypothyroid than diabetic rats. Serum T3 level decreased in order in diabetic (- 26.63%), hypothyroid (- 61.89%) and diabetic hypothyroid (- 65.69%) rats while T4 level was increased in diabetic rats and decreased in hypothyroid ones. The decrease in T3 level in diabetic animals in spite of T4 increase may be attributed to the decrease in conversion of T4 to T3 as a result of hepatic 5′-DI decreased activity. Liver glycogen content was three-fold decreased in diabetic rats and was not significantly altered in both hypothyroid and diabetic hypothyroid rats. The serum leptin level and body weight gain were decreased in diabetic and diabetic hypothyroid rats; the leptin level was more deteriorated in diabetic hypothyroid rats while body weight gain was more affected in diabetic rats. Serum triglycerides level was more increased in diabetic rats than in diabetic hypothyroid ones on one hand, while total lipids, total cholesterol, LDL-cholesterol levels as well as cardiovascular indices were more deteriorated in diabetic hypothyroid rats than diabetic ones on the other hand. Serum total protein and globulin levels were decreased in diabetic rats and were increased in hypothyroid and diabetic hypothyroid rats. Hepatic total thiols and glutathione contents and catalase and peroxidase activity were profoundly decreased in diabetic rats while they (except glutathione) were increased in hypothyroid and diabetic hypothyroid rats. In conclusion, the hypothyroidism may have a counteracting effect on the hyperglycemic state and the elevated serum T4 level as well as the deteriorated antioxidant defense system found in diabetes mellitus, but both experimentally-induced diseases may synergize in inducing more elevation of serum total lipids, total cholesterol and LDL-cholesterol and more decrease in leptin levels. © 2012 Elsevier Inc.


Elazab S.,Nahda University | Alazab M.,Higher Technological Institute
Proceedings - 2015 5th International Conference on e-Learning, ECONF 2015 | Year: 2015

Recent communication and information technology have unlocked entirely new directions for education and training. Mounting pressure from increasing free, online course offerings is opening discussion and catalyzing change in the physical classroom. The flipped classroom is at the center of this arena. The flipped classroom is a new pedagogical method, which employs asynchronous video lectures and practice problems as homework, and active, group-based problem solving activities in the classroom. It represents a unique combination of learning theories once thought to be incompatible-active, problem-based learning activities founded upon a constructivist ideology and instructional lectures derived from direct instruction methods founded upon behaviorist principles. This paper provides a comprehensive survey of prior and ongoing research of the flipped classroom and introduces a case study for higher education and the perception of the both students and instructors. Studies are characterized on the type of in-class and out-of-class activities, On the other hand, we introduce some notes to help faculties to smoothly transform from traditional classroom to flipped classroom. © 2015 IEEE.


Radwan A.A.,Minia University | El-Bakry H.M.,Mansoura University | El Hadad H.M.,Nahda University
Recent Researches in Applied Informatics - Proceedings of the 2nd International Conference on Applied Informatics and Computing Theory, AICT'11 | Year: 2011

The successful application of data mining in highly visible fields like e-business and marketing have led to the popularity of its use in knowledge discovery in databases (KDD) in other industries and sectors. Among these sectors that are just discovering data mining are the fields of medicine and public health. The medical industries collect huge amounts of healthcare data which, unfortunately, are not "mined" to discover hidden information. We can describe this data as being 'information rich' yet 'knowledge poor'. In this study, we briefly examine the use of the most important data mining techniques such as Artificial Neural Network to massive volume of data in medical field which is pediatric respiratory disease. Using medical symptoms such as dry cough, productive cough, fever, heamoptysis, tachypnea, dysnea and etc. Also using doctor sign such as bronchial breathing, chest pain, clubbing of finger, crepitation, ronchi, cyanosis, decrease brearthing sound on auscultation, dullness on percussion, hyper resonant on percussion, inability to swallow, mucopurelent sputum, pleural rub, respiratory distress, sputum (white), stridor, upper respiratory infection, wheezing, X-ray [showing lung consolidation], X-ray [shawing edematous epiglottic], X-ray [shawing subglottic narrowing and classic narrow trachea], X-ray [showing hypertanslucent lung], X-ray [showing lobar collapse and (increase) bronchovascular marking], X-ray [showing diffuse haziness], it can predict the likelihood of patients getting a respiratory disease. They enable significant knowledge, e.g. patterns, relationships between medical factors related to respiratory disease, to be established.


Khorshid A.F.,Nahda University | Issa Y.M.,Cairo University
Biosensors and Bioelectronics | Year: 2014

A novel, simple, rapid, selective and sensitive method for the determination of neostigmine (Ns) ion in its bulk powder, different pharmaceutical dosage forms, and biological fluids (plasma and urine) using four modified carbon paste electrodes was developed. Sensor 1 is based on ion-association Ns-TPB, sensor 2 used Ns-PT, sensor 3 comprises a mixture of (Ns-PT+Ns-TPB) and sensor 4 was constructed using (Ns-PT+β-CD). Solvent mediator 2-NPPE exhibited a proper behavior including Nernstian slope ranging from 61.5±0.5 to 64.5±0.5mV per decade over the pH range of 3.8-10 for the four sensors. Linear responses of Ns within the concentration range 1.0×10-7-1.0×10-2mol/L were obtained. The response time is very short (≤10s) with a detection limit 6.3×10-8M. In flow injection analysis (FIA), sensor 3 shows a Nernstian slope value 75.5±0.5mV per decade within the concentration range of 1×10-6-1×10-2mol/L and with a detection limit 7.5×10-7 mol/L. The utility of mixed or additives of β-CD had a significant influence on increasing the sensitivity of sensors 3 and 4 compared to sensors 1 and 2. The sensors were applied for the determination of neostigmine (Ns) ion in its bulk powder, different pharmaceutical dosage forms, and biological fluids (plasma and urine). The results obtained were satisfactory with excellent percentage recovery comparable with official method for the assay based on non-aqueous titration using perchloric acid as a titrant. © 2013 Elsevier B.V.


The fabrication and the performance response characteristics of a sensitive, selective, simple, and rapid sensor for the determination of clopidogrel bisulfate (CLO-H2SO4) were described. The constructed carbon paste sensor comprised of an ion-pair based on clopidogrel with silicotungstate (CLO-ST) where this study included: composition, usable pH range, response time and temperature. The sensor exhibited a wide linear dynamic concentration ranging from 1.00×10-7 to 1.00×10-2 and the usable pH ranges from 1.2 to 4.8 with the response time ranging from 5 to 8s which is much faster compared to liquid ISEs with a detection limit equalling 0.34nM. The selectivity of the sensor (CLO-H2SO4) was applied with respect to many of organic and inorganic cations, amino acids and sugars. The sensor had applications in bulk powder, tablets, humans (serum-urine) and in monitoring Plavix tablets' dissolution rates. The obtained results were statistically analyzed for both accuracy and precision and were compared using the US pharmacopeial method where no significant difference was observed. © 2014 King Saud University.


El-Sherbiny G.M.,October 6 University | el Sherbiny E.T.,Nahda University
Iranian Red Crescent Medical Journal | Year: 2011

Background: Trichomoniasis vaginalis is now an important worldwide health problem. Metronidazole has so far been used in treatment, but the metronidazole-resistant strains and unpleasant adverse effects have been developed. Myrrh is one of the oldest known medicinal plants used by the ancient Egyptians for medical purposes and for mummification. Commiphora molmol (Myrrh) proved safe for male reproductive organ which is the main habitat of T. vaginalis and this study aims to evaluate the efficacy of the herbal against T. vaginalis in females. Methods: In the present study, 33 metronidazole-resistant T. vaginalis females were treated with a combined course of metronidazole and tinidazole. Those still resistant to the combined treatment were given C. molmol. Also, natural plant extract purified from pomegranate (Punica granatum, Roman) was in-vitro investigated for its efficacy against T. vaginalis on Diamond media. Results: The anti-T. vaginalis activity of both P. granatum (in-vitro) and C. molmol (in-vivo) extracts gave promising results. Conclusion: The anti-T. vaginalis activity of P. granatum and C. molmol showed promising results indicating to sources of new anti-Ttrichomonas agents. © Iranian Red Crescent Medical Journal.


Anodic oxidation of Co, Cu, Zn, and Sn metals in an anhydrous acetone solution of 1,1-oxalyl-, malonyl- or succinyl-bis(4-p- chlorophenylthiosemicarbazide) yields a new polynuclear complexes. The isolated complexes have the general composition [M 2(L)(H 2O) 6], L = pClSuTS and M = Co(II), Cu(II) or Sn(II), [M 2(L)(H 2O) n]·nH 2O where M = Cu(II), Co(II) or Sn(II), L = pClOxTS and n = 2 or 6, and [M 2(L)(ac) 2]·nH 2O where M = Co(II) or Zn(II), L = pClOxTS or pClSuTS and n = 2. The thermogravimetry (TG) and derivative thermogravimetry (DTG) have been used to study the thermal decomposition of the investigated bisthiosemicarbazide ligands and their metal complexes. The kinetic thermodynamic parameters such as: E, ΔH, ΔS and ΔG are calculated using Horowitz-Metzger (HM) and Coats-Redfern (CR) equations. The kinetic thermodynamic parameters such as: E, ΔH, ΔSand ΔG are calculated from the DTG curves. The tested compounds show a reasonable activity against four strains of Gram-negative bacteria; Escherichia coli, Pseudomonas aeruginosa species and Gram-positive bacteria; Bacillus cereus and Staphylococcus aureus. © 2011 Elsevier B.V. All rights reserved.

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