Kale D.S.,National Dairy Research Institute |
Kale D.S.,Nagpur Veterinary College |
Yadav B.R.,National Dairy Research Institute
Indian Journal of Animal Sciences | Year: 2011
DNA polymorphism of Butyrophilin gene was analyzed using PCR-RFLP, SSCP and to find possible relationship (if any) of identified polymorphism with milk production traits in Murrah buffalo. Genomic DNA was isolated from 110 Murrah from Institute's herd. The PCR-RFLP polymorphism analysis of Butyrophilin gene using TaqI, MboI and HaeIII restriction enzymes revealed single restriction pattern indicating absence of variation in buffalo. The PCR-SSCP analysis of intron 3 and 4 exhibited monomorphic SSCP patterns. However, intron 1 region of Butyrophilin revealed polymorphic BTI1SSCP with 3 patterns. The identified buffalo butyrophilin variants were found with following frequencies: A, 0.6; B, 0.31 and C, 0.09 in Murrah buffaloes. The direct DNA sequencing of 3 polymorphic SSCPs within Murrah buffalo Butyrophilin gene revealed BTI1 SNP (c.184C>T>G) confirming 3 variants, viz. A, B and C. The statistical analysis indicated BTI1TT SNP genotypes were significantly associated with 305 days lactation milk yield of Murrah buffaloes. The Murrah buffaloes with BTI1TT SNP genotypes had 683.93 kg and 320.48 kg higher milk yield as compared to BTI1CT and BTI1CG SNP genotypes respectively. The identified association of BTI1TT SNP genotypes with milk yield after validation in large population with more records will be useful for designing selection and breeding strategies for genetic improvement of buffaloes for milk production.
Kharkar K.,Post Graduate Institute of Veterinary and Animal science |
Kharkar K.,Nagpur Veterinary College |
Kuralkar S.V.,Post Graduate Institute of Veterinary and Animal science |
Hadge M.R.,Post Graduate Institute of Veterinary and Animal science
Indian Journal of Animal Research | Year: 2015
The data on 481 lactation records including 204 records of Red Kandhari cows and 277 records of Jersey x Red Kandhari crossbred cows were collected from Cattle Unit, College of Veterinary and Animal Sciences, (MAFSU), Parbhani over a period of 27 years (1977-2004). The lactation milk yield for first, second, third, fourth and fifth and above lactations for Red Kandhari cows were 375.16±34.15 kg, 458.17±37.62 kg, 450.91±26.74 kg, 532.69±67.74 kg and 674.29±82.09 kg, respectively and for Jersey x Red Kandhari crossbred were 1100.28±73.18 Kg, 1318.50±135.20 Kg and 1353.92±91.61 Kg, 1549.77±188.47 kg and 1438.37±48.89 kg, respectively. The first lactation length and first dry period in Red Kandhari cows were 273.30±10.33 days and 239.05±21.88 days, while in Jersey x Red Kandhari crossbred cows were 302.68±9.19 days and 86.72±8.90 days, respectively. The least square analysis of variance for first, second and third and more than three lactations for lactation milk yield, lactation length and dry period showed that the genetic group had significant source of variation for all traits in all lactations except first and second lactation length. Sire within genetic group had significant source of variation for first lactation milk yield and all traits of third and more than three lactations. Season and period of calving had non-significant influence on all the traits in all lactations except first dry period. It was also observed that the improved performance in all the traits under study in Red Kandhari cow and Jersey x Red Kandhari crossbred cow was observed in fourth and fifth and above lactations. © 2015, Agricultural Research Communication Centre. All rights reserved.
Yedke S.,Yashwant Ayurved College |
Raut S.,Government Ayurved College |
Jangde C.,Nagpur Veterinary College
Journal of Ayurveda and Integrative Medicine | Year: 2013
Background: Success of surgery depends on wound closure and healing. Ancients had coated many suture materials from plant and animal origin. As the quest for natural nonabsorbable, monofilament surgical suture continues, horsehair has been taken for study, which is mentioned in ancient literature. Objectives: Aim of the study was to evaluate detail mechanical and biophysical properties of horsehair. Materials and Methods: Physical properties, that are diameter, straight pull and knot pull tensile strength, bioburden, sterility tests were performed. Visual and histological wound healing parameters were studied in experimental Wistar rat incision wound model. Two experimental wounds about 5 cm long were created on each side of dorsal midline. Each animal received two sutures-Horsehair 4-0 and Ethilon 4-0. The sutured areas were grossly examined on 3 rd and 7 th days for visual observations like congestion, edema, infection, wound disruption, and impression of suture material on healed wound and then subjected for histological study. Results: Revealed that horsehair has got diameter of 0.19 mm which complies with the 4-0 size USP standard. Straight pull tensile strength was found 0.5851 ± 0.122 kg and knot pull tensile strength was 0.3998 ± 0.078 kg, which complies with the standards of United State Pharmacopia for class II nonabsorbable suture materials. In vivo study revealed that there was no evidence of edema, congestion, and discharge in both the groups. Wounds healed with minimum impressions of suture material with minimum scar mark. Mean histological scoring shows very mild tissue reaction. Conclusion: Horsehair has got properties of standard suture material except low tensile strength and hence can be used in reconstructive, plastic surgeries, and ophthalmic surgeries.
Mohod P.S.,Rajiv Gandhi Ayurved College |
Jangde C.R.,Nagpur Veterinary College |
Narnaware S.D.,National Research Center on Camel |
Raut S.,Government Ayurved College
Journal of Applied Pharmaceutical Science | Year: 2014
The aqueous and alcoholic extracts of bark skin of Saraca indica (Ashoka) and Shorea robusta (Shal) were evaluated for their analgesic activity in Swiss albino rats by making use of different pain models such as, tail immersion test, tail clip method and writhing induced by 4% NaCl solution. The aqueous and alcoholic extract of Saraca indica and Shorea robusta showed significant analgesic activity at 300 mg/ Kg body weight in Swiss albino rats as compared with control rats from physical, thermal and chemical stimulus of evaluation techniques. The analgesic activity might have been attributed to the presence of alkaloids, steroids in these plants as revealed from phytochemical analysis. On the basis of these observations it was concluded that Ashoka and Shal has got analgesic property, however further experimental as well as clinical evaluations are necessary. © 2014 Poonam S. Mohod et al.
Basunathe V.K.,Nagpur Veterinary College |
Sawarkar S.W.,Nagpur Veterinary College |
Sasidhar P.V.K.,University for Development Studies
Outlook on Agriculture | Year: 2010
This paper describes the uptake of five dairy production technologies - artificial insemination, deworming, vaccination, concentrate feeding and mineral mixture feeding - in the Nagpur district of India's Vidarbha region. The survey was carried out in 21 villages and data were collected from 210 dairy farmers. The major reasons for the non-adoption or discontinuation of these technologies were: non-availability of service; lack of knowledge among farmers; and withdrawal of free/subsidized services by government. The implications for dairy development are discussed.