Nāgpur, India
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Kolte S.W.,Nagpur Veterinary College | Larcombe S.D.,University of Glasgow | Jadhao S.G.,Nagpur Veterinary College | Magar S.P.,Nagpur Veterinary College | And 4 more authors.
PLoS ONE | Year: 2017

Tick-borne pathogens (TBP) are responsible for significant economic losses to cattle production, globally. This is particularly true in countries like India where TBP constrain rearing of high yielding Bos taurus, as they show susceptibility to acute tick borne disease (TBD), most notably tropical theileriosis caused by Theileria annulata. This has led to a programme of cross breeding Bos taurus (Holstein-Friesian or Jersey) with native Bos indicus (numerous) breeds to generate cattle that are more resistant to disease. However, the cost to fitness of subclinical carrier infection in crossbreeds relative to native breeds is unknown, but could represent a significant hidden economic cost. In this study, a total of 1052 bovine blood samples, together with associated data on host type, sex and body score, were collected from apparently healthy animals in four different agro-climatic zones of Maharashtra state. Samples were screened by PCR for detection of five major TBPs: T. annulata, T. orientalis, B. bigemina, B. bovis and Anaplasma spp. The results demonstrated that single and co-infection with TBP are common, and although differences in pathogen spp. prevalence across the climatic zones were detected, simplistic regression models predicted that host type, sex and location are all likely to impact on prevalence of TBP. In order to remove issues with autocorrelation between variables, a subset of the dataset was modelled to assess any impact of TBP infection on body score of crossbreed versus native breed cattle (breed type). The model showed significant association between infection with TBP (particularly apicomplexan parasites) and poorer body condition for crossbreed animals. These findings indicate potential cost of TBP carrier infection on crossbreed productivity. Thus, there is a case for development of strategies for targeted breeding to combine productivity traits with disease resistance, or to prevent transmission of TBP in India for economic benefit. © 2017 Kolte et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Majumdar S.,Central Avian Research Institute | Bhanja S.K.,Central Avian Research Institute | Mandal A.B.,Central Avian Research Institute | Kadam M.,Nagpur Veterinary College
Journal of Applied Animal Research | Year: 2017

Two hundred turkey breeder hens and 24 viable toms of 30-35 weeks (wk) old of small white variety were distributed into 2 treatment groups having 4 replicates First four replicates were offered diet A [National Research Council. 1994. Nutrient requirements of poultry. 8th ed. Washington, DC: National Academy Press] and other four replicates were maintained on a higher plane of nutrition – diet B for 8 wk. Five hundred and fortyeight fertile eggs on 21st embryonic day were in ovo injected with nutrients (essential amino acids - INA; linolenic acid, linoleic acid, retinol and DL-alpha-tocopherol-INFV; INA + INFV-INAFV, sham control – S and un-injected control – C). INAFV poults had significantly (P < .01) higher body weight compared to other treatment groups till 8 wk of age. Total immunoglobulins in response to 1% sheep red blood cells were significantly higher (P < .01) in the INA group compared to the C group. Cell-mediated immune response was significantly higher (P < .01) in the diet B group compared to diet A group. Thus, INAFV treatment may be carried out for better posthatch growth and breeders may be maintained on higher plane of nutrition along with INA treatment to elicit better post-hatch immunity. © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group


Lahon A.,National Institute of Virology | Ingle V.C.,Nagpur Veterinary College | Raut C.G.,National Institute of Virology | Chitambar S.D.,National Institute of Virology
Veterinary Microbiology | Year: 2014

The occurrence of group B rotavirus (RVB) infections in pigs has been reported from different parts of world. However, such infection in the pig population maintained in Indian farms has not been investigated as yet. A total of 187 faecal specimens were collected from pigs reared in different pig farms/pigsties located in western and northern regions of India and tested for the presence of porcine RVB by amplification of the NSP2 gene using conventional RT-PCR. Nine specimens (4.8%) were shown to contain RVB RNA. N2 and N4 genotypes of NSP2 gene were detected in three and six RVB strains respectively. VP7 (G-type) and NSP5 (H-type) genes of selected six RVB strains were characterized to identify the genotypes. Multiple G (G7, G19 and G20) and H (H4 and H5) genotypes detected in the RVB strains indicated circulation of heterogeneous population of RVB strains in pigs of India. Additionally, one strain was proposed to belong to a novel RVB genotype designated as G21 on account of <80% identity of VP7 gene sequence with its counterpart in RVB strains from 20 established genotypes. Deduced amino acid sequence of VP7 gene also displayed the presence of seven unique substitutions in the strain. The study reports for the first time the occurrence of RVB infections in Indian pig herds and provides important epidemiological data useful for better understanding of ecology and evolution of porcine RVBs. © 2014 Elsevier B.V.


Husain A.A.,Central India Institute of Medical science | Kashyap R.S.,Central India Institute of Medical science | Kalorey D.R.,Nagpur Veterinary College | Warke S.R.,Nagpur Veterinary College | And 3 more authors.
Indian Journal of Experimental Biology | Year: 2011

BCG is the only vaccine presently available against tuberculosis but it is estimated to prevent only 5% of the all potentially vaccine-preventable deaths due to Tuberculosis. Keeping these in view the present study has been undertaken to evaluate the efficacy of BCG and the effect of repeat dose of BCG on antimycobacterial humoral response in mouse model. To improve BCG immunogenicity, specific anti-mycobacterial immune responses (anti-BCG titre and total IgG level) were evaluated in mouse model using boost immunization protocols with the BCG vaccine. Mice induced with a repeat dose of BCG showed an increased anti mycobacterial humoral response, which gradually declined few weeks after single dose of BCG administration. The results suggest improved efficacy of BCG vaccine by giving repeat dose of BCG that can enhance the level of immunoprotection against tuberculosis as opposed to a single BCG dose.


PubMed | Nagpur Veterinary College, Central India Institute of Medical science and Indian National Environmental Engineering Research Institute
Type: Journal Article | Journal: Immune network | Year: 2015

Evaluation and screening of vaccines against tuberculosis depends on development of proper cost effective disease models along with identification of different immune markers that can be used as surrogate endpoints of protection in preclinical and clinical studies. The objective of the present study was therefore evaluation of subcutaneous model of M.tuberculosis infection along with investigation of different immune biomarkers of tuberculosis infection in BALB/c mice. Groups of mice were infected subcutaneously with two different doses : high (210(6) CFU) and low doses (210(2) CFU) of M.tuberculosis and immune markers including humoral and cellular markers were evaluated 30 days post M.tuberculosis infections. Based on results, we found that high dose of subcutaneous infection produced chronic disease with significant (p<0.001) production of immune markers of infection like IFN, heat shock antigens (65, 71) and antibody titres against panel of M.tuberculosis antigens (ESAT-6, CFP-10, Ag85B, 45kDa, GroES, Hsp-16) all of which correlated with high bacterial burden in lungs and spleen. To conclude high dose of subcutaneous infection produces chronic TB infection in mice and can be used as convenient alternative to aerosol models in resource limited settings. Moreover assessment of immune markers namely mycobacterial antigens and antibodies can provide us valuable insights on modulation of immune response post infection. However further investigations along with optimization of study protocols are needed to justify the outcome of present study and establish such markers as surrogate endpoints of vaccine protection in preclinical and clinical studies in future.


PubMed | Nagpur Veterinary College and Central India Institute of Medical science
Type: Journal Article | Journal: Clinical and experimental vaccine research | Year: 2015

In the present study booster efficacies of Ag85 B, Bacillus Calmette-Guerin (BCG), and Ag85B peptides were evaluated using prime boost regimes in BALB/c mice.Mice were primed with BCG vaccine and subsequently boosted with Ag85B, BCG and cocktail of Ag85B peptides.Based on analysis of immune response it was observed mice boosted with Ag85B peptides showed significant (p < 0.001) cytokines levels (interferon , interleukin 12) and BCG specific antibodies (anti-BCG and anti-purified protein derivative titre) compared to booster dose of BCG, Ag85B and BCG alone.Our pilot results suggest that prime boost regimes with Ag85B peptides can boost waning BCG induced immunity and may improve immunogenicity of BCG vaccine. However, lot of work is further needed using experimental model of tuberculosis infection to justify the result.


Yedke S.,Yashwant Ayurved College | Raut S.,Government Ayurved College | Jangde C.,Nagpur Veterinary College
Journal of Ayurveda and Integrative Medicine | Year: 2013

Background: Success of surgery depends on wound closure and healing. Ancients had coated many suture materials from plant and animal origin. As the quest for natural nonabsorbable, monofilament surgical suture continues, horsehair has been taken for study, which is mentioned in ancient literature. Objectives: Aim of the study was to evaluate detail mechanical and biophysical properties of horsehair. Materials and Methods: Physical properties, that are diameter, straight pull and knot pull tensile strength, bioburden, sterility tests were performed. Visual and histological wound healing parameters were studied in experimental Wistar rat incision wound model. Two experimental wounds about 5 cm long were created on each side of dorsal midline. Each animal received two sutures-Horsehair 4-0 and Ethilon 4-0. The sutured areas were grossly examined on 3 rd and 7 th days for visual observations like congestion, edema, infection, wound disruption, and impression of suture material on healed wound and then subjected for histological study. Results: Revealed that horsehair has got diameter of 0.19 mm which complies with the 4-0 size USP standard. Straight pull tensile strength was found 0.5851 ± 0.122 kg and knot pull tensile strength was 0.3998 ± 0.078 kg, which complies with the standards of United State Pharmacopia for class II nonabsorbable suture materials. In vivo study revealed that there was no evidence of edema, congestion, and discharge in both the groups. Wounds healed with minimum impressions of suture material with minimum scar mark. Mean histological scoring shows very mild tissue reaction. Conclusion: Horsehair has got properties of standard suture material except low tensile strength and hence can be used in reconstructive, plastic surgeries, and ophthalmic surgeries.


PubMed | Indian Veterinary Research Institute, Nagpur Veterinary College, National Institute of Biotic Stress Management and Justus Liebig University
Type: | Journal: Journal of microbiological methods | Year: 2016

A novel multiplex PCR assay was developed to identify genus Listeria, and discriminate Listeria monocytogenes and its major lineages (LI, LII, LIII). This assay is a rapid and inexpensive subtyping method for screening and characterization of L. monocytogenes.


Basunathe V.K.,Nagpur Veterinary College | Sawarkar S.W.,Nagpur Veterinary College | Sasidhar P.V.K.,University for Development Studies
Outlook on Agriculture | Year: 2010

This paper describes the uptake of five dairy production technologies - artificial insemination, deworming, vaccination, concentrate feeding and mineral mixture feeding - in the Nagpur district of India's Vidarbha region. The survey was carried out in 21 villages and data were collected from 210 dairy farmers. The major reasons for the non-adoption or discontinuation of these technologies were: non-availability of service; lack of knowledge among farmers; and withdrawal of free/subsidized services by government. The implications for dairy development are discussed.


Mahajan V.E.,Nagpur Veterinary College | Yadav R.R.,Indian National Environmental Engineering Research Institute | Dakshinkar N.P.,Nagpur Veterinary College | Dhoot V.M.,Nagpur Veterinary College | And 5 more authors.
Environmental Monitoring and Assessment | Year: 2012

Cattle grazing nearby coal-fired power stations are exposed to fly ash. The present investigation aims to assess the environmental and health impacts of fly ash containing mercury emitted from thermal power plant. The health effect of fly ash were studied using 20 lactating cattle reared within a 5-km radius of s thermal power plant for the possible effect of fly ash such as the alterations in hematological and biochemical parameters of blood, milk, and urine. Results indicated that the hemoglobin levels (6.65±0.40 g/dl) were significantly reduced in all the exposed animals. Biochemical parameters viz., blood urea nitrogen (27.35± 1.19 mg/dl), serum glutamate oxaloacetate transaminase (43.39±3.08 IU/l), albumin, and creatinine were found to be increased, whereas serum glutamate pyruvic transaminase (29.26±2.02) and Ca2+ were observed to be statistically insignificant in exposed animals. Mercury concentrations estimated in the blood, milk, and urine of exposed (n020) and control (n020) animals were 7.41±0.86, 4.75±0.57, 2.08±0.18, and 1.05±0.07, 0.54±0.03, 0.20±0.02 μg/kg, respectively. The significant increase (P<0.01) in the levels of mercury in blood, milk, and urine of exposed animals in comparison to control indicated that the alterations of biochemical parameters in exposed cattle could be due to their long term exposure to fly ash mercury which may have direct or indirect impact on human populations via food chain. © Springer Science+Business Media B.V. 2012.

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