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Hibino S.,Nagoya City Rehabilitation Center | Ueda N.,Nagoya University | Horiba M.,Suizawa Hospital | Yasui K.,Sakae KEN Heart Clinic | And 4 more authors.
Circulation Journal

Background: To establish a simple and accurate method for the automated identification of the end of a T wave, we approximated electrocardiograph (ECG) traces using a Gaussian mixture model in conjunction with a split-andmerge expectation-maximization algorithm. Methods and Results: A total of 286 ECG traces of heart beats of 50 healthy men were used as control data and ECGs from 15 subjects recorded before and after 400 mg oral moxifloxacin as positive controls. An experienced cardiologist determined the reference points by visual inspection of the original ECGs. The primary estimated point for the end of the T wave was selected as the point 2 ms before the point at which the gradient of the approximated wave was not steeper than the common threshold value. This point was then adjusted by applying modification rules proposed by an experienced cardiologist. The absolute value of the average interval between the resulting final estimated point and the manually selected reference point was 1.8±7.7 ms for the control data. After treatment with moxifloxacin, the average QT interval, corrected by Bazett's formula, showed a 17.2±27.1 ms prolongation with a lower bound of the 95% confidence interval of 4.9 ms. Conclusions: When the modification rules were applied, the accuracy of QT measurement was improved, and the present system was capable of detecting QT prolongation correctly. Source

Hibino S.,Nagoya City Rehabilitation Center | Nakatochi M.,Nagoya University | Ueda N.,Nagoya University | Horiba M.,Nagoya University | And 7 more authors.
Transactions of Japanese Society for Medical and Biological Engineering

The purpose of this study is to establish the accurate automated QT measurement system for detecting drug-induces QT interval prolongation. One hundred and thirty five electrocardiograms (ECGs) were recorded from fifty healthy men. We applied Gaussian mixture model to approximate the ECG trace with split and merge expectation maximization (SMEM) algorithm for optimal model parameters. Lead II ECG trace from the beginning of a T wave to the end of subsequent beats P wave was approximated by six Gaussian functions with SMEM algorithm. The end of T wave from original ECG was registered by an experienced cardiologist and corresponding point on the approximated ECG trace was defined as a reference point. A primary estimated point for the end of T wave was selected by using a threshold that was the averaged height differences between the reference point and the 2 ms preceding point. The averaged interval in absolute value between the estimated point and the reference point was 5.4 ± 4.3 ms. Furthermore, it was diminished to 5.0 ± 3.8 ms by multiple regression model analysis (Dependent variable: the reference point as the end of T wave. Independent variables: the primary estimated point as the end of T wave and the each height associated with the fourth and the sixth Gaussian function). The final estimated QT interval and the reference QT interval were 399.2 ± 27.2 ms and 399.2 ± 28.0 ms, respectively. We conclude that our system is able to measure QT interval correctly. Source

Suzuki S.,Nagoya City University | Suzuki S.,Nagoya City East Medical Center | Shiraga K.,Nagoya City University | Sato S.,Nagoya City University | And 7 more authors.
Cancer Science

Recent evidence suggests that oxidative stress contributes to the pathogenesis of prostate cancer. The present study focused on the effect of apocynin, an inhibitor of NADPH oxidase, on prostate carcinogenesis using the transgenic rat for adenocarcinoma of prostate (TRAP) model. There were no toxic effects with apocynin treatment. The percentages and numbers of carcinomas in both the ventral and lateral prostate were significantly reduced by apocynin treatment, with dose dependence. Reduction of reactive oxygen species by apocynin was confirmed by immunohistochemistry of 8-OHdG and dihydroethidium staining. Positivity of Ki67 was significantly reduced by apocynin treatment, and downregulation of clusterin expression, as well as inactivation of the MEK-ERK1/2 pathway, was a feature of the apocynin treated groups. In human prostate cancer cell line LNCaP, apocynin also inhibited reactive oxygen species production and blocked cell growth by inducing G0/G1 arrest with downregulation of clusterin and cyclin D1. These data suggest that apocynin possesses chemopreventive potential against prostate cancer. © 2013 Japanese Cancer Association. Source

Tokudome S.,Nagoya City University | Tokudome S.,National Institute of Health and Nutrition | Tokudome S.,Social Welfare Institutions Seizanri kai | Kuriki K.,Nagoya City University | And 20 more authors.
Prostaglandins Leukotrienes and Essential Fatty Acids

To address preventive effects of n-3 PUFAs/LC n-3 PUFAs on CRTs, a randomized controlled trial was conducted. One-hundred four experimental group participants were advised to increase intake of n-3 PUFAs, including fish/shell fish, fish oil supplements and perilla oils, and to decrease consumption of n-6 PUFAs and fats/oils as a whole for 24 months. One-hundred one control group participants were only cautioned to reduce consumption of fats/oils as a whole. Random allocation was satisfactorily attained, and participants sufficiently complied with our regimen. Intakes, plasma concentrations, and compositions of the RBC and sigmoid colon membranes of n-3 PUFAs, LC n-3 PUFAs, EPA and DHA increased, and the ratios of n-6 PUFAs/n-3 PUFAs and AA/LC n-3 PUFAs decreased without any adverse response. Twenty-four months after the intervention, the multivariate-adjusted hazard ratio (95% confidence intervals) was estimated to be 0.805 (0.536-1.209) with a signal towards the reduced CRT incidence. © 2014 Elsevier Ltd. Source

Hibino S.,Nagoya City Rehabilitation Center | Mase M.,Nagoya City University | Shirataki T.,Nagoya City Rehabilitation Center | Nagano Y.,Nagoya City Rehabilitation Center | And 10 more authors.
Neurologia Medico-Chirurgica

To investigate cerebral reactions to cognitive rehabilitation tasks, oxyhemoglobin changes were compared in 9 patients with cognitive impairments after traumatic brain injury (TBI) and 47 healthy controls using functional near infrared spectroscopy (fNIRS) during nine cognitive rehabilitation tasks employed at Nagoya City Rehabilitation Center. Forty-seven measurement channels were placed on the frontal to temporal cortices, and organized into seven channel regions. Oxyhemoglobin changes were normalized based on the mean oxyhemoglobin value at the resting state, and integrated throughout a task. Statistical analyses of the differences between the TBI patients and controls were performed with the two-sided Mann-Whitney U test. Oxyhemoglobin changes were high for both controls and TBI patients in the lateral frontal regions. Oxyhemoglobin changes in TBI patients tended to be higher than controls in the medial frontal regions for most training tasks, and significant differences (p < 0.05) were seen for two tasks in the medial frontal regions. Different regions were activated during the tasks in TBI patients compared to controls. fNIRS measurement is useful in the evaluation of changes of neuronal activities during rehabilitation tasks in TBI patients. Source

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