Time filter

Source Type

Watanabe S.-I.,Japan National Agriculture and Food Research Organization | Inoue K.,Nagasaki Agricultural and Forestry Technical Development Center | Emoto S.,Forestry and Fisheries Research Center | Ishibashi Y.,Saga Prefectural Agriculture Research Center | And 2 more authors.
Japan Agricultural Research Quarterly | Year: 2014

Kyushu island, in southern Japan, is one of the main producers of green asparagus (Asparagus officinalis L.) in Japan, accommodating protected mother-fern culture in plastic greenhouses (PMC) as a conventional crop. In PMC, since few spears are produced from November to January, we attempted to produce spears during this period by prolonging the harvest of PMC to November and introducing "Fusekomi" forcing culture (FFC), in which rootstocks grown outdoors are transplanted into heated and/or thermal-retained culture beds in a plastic greenhouse in late autumn or early winter and spears are generally harvested for the following two or three months. In PMC, a combination of a foliage spray of Benzyladenine solution with thermal retention treatment in autumn prolonged the harvest period to the end of November without any significant decrease in yield the following spring. In FFC, rootstocks grown in the lowlands showed inferior spear productivity when transplanted into a greenhouse in late November, probably due to insufficient exposure to low temperature for breaking dormancy. In contrast, rootstocks grown in the highlands showed superior in productivity because they would be sufficiently exposed to low temperature for breaking dormancy by the time of transplanting. In lowlands, heating of greenhouses was unnecessary for FFC throughout the harvest period due to the mild winter climate. These results suggested that profitable year-round production of green asparagus without heating could be realized in Kyushu by combining PMC and FFC cropping types.


Mori K.,Nagasaki Agricultural and Forestry Technical Development Center | Asano K.,Japan National Agriculture and Food Research Organization | Tamiya S.,Japan National Agriculture and Food Research Organization | Nakao T.,Nagasaki Agricultural and Forestry Technical Development Center | Mori M.,Japan National Agriculture and Food Research Organization
Breeding Science | Year: 2015

The potato (Solanum tuberosum L.) is cultivated all year round in Japan by using four types of cropping: summer and winter croppings, and double cropping in spring and fall. In each cropping season, growth conditions such as temperature, day length, and growing period, differ drastically; thus, different cultivars adapted to each environment are required. Breeding stations are located in both summer cropping areas and double cropping areas, and cultivars suitable for each cropping system are developed. The required cultivars differ according to cropping type and according to use such as table use, food processing, and starch production. The qualities necessary for each purpose differ and are therefore evaluated accordingly. Improvements in pest and disease resistance and in yield abilities are important as common breeding targets for all purposes. To develop potato cultivars that meet different needs, breeders have continued efforts to improve these traits. In this review, we introduce our approaches to developing new potato cultivars. We also discuss problems predicted in the future and introduce our efforts on broadening genetic diversity. © 2015, Japanese Society of Breeding. All rights reserved.


Fukagawa S.,Nagasaki Agricultural and Forestry Technical Development Center | Ishii Y.,University of Miyazaki | Sato K.,Japan National Agriculture and Food Research Organization | Kobayashi R.,Japan National Agriculture and Food Research Organization | Hattori I.,Japan National Agriculture and Food Research Organization
American Journal of Plant Physiology | Year: 2014

Most tropical grasses in the warm southwestern region of Japan are cultivated as annuals and can be sown from May to July, after the harvest of an alternating crop of Italian ryegrass. However, suboptimal thermal conditions in the region may affect the germination and growth of seedlings. The objective of this study was to compare the growth of seedlings of promising tropical grasses in a Controlled Environment Facility (CEF) and in a Field Data (FD) trial. Temperatures in the CEF averaged 17.9, 22.9 and 27.9°C for the low (LT), middle (MT) and high temperature (HT) regimes, respectively, while the temperature in the FD trial was higher in the June-sowing (average 23.7°C, with a range between MT and HT in the CEF) than in the May sowing (average 21.2°C, between LT and MT). Favorable plant growth attributes tended to increase with the increase in air temperature in both trials. The growth rates in Guinea grass and Sudan grass exhibited a linear response to the increase in temperature from LT to HT, whereas the growth rate of Rhodes grass and colored Guinea grass showed saturation between MT and HT. Thus, for early sowing in mid-May, the prominent species judged in terms of high seedling potential were Sudan grass and Rhodes grass and for the late-sowing in early July, Sudan grass and Guinea grass. © 2014 Academic Journals Inc.


Horita M.,Japan National Institute for Agro - Environmental Sciences | Suga Y.,Nagasaki Agricultural and Forestry Technical Development Center | Ooshiro A.,Okinawa Prefectural Agricultural Research Center | Tsuchiya K.,Kyushu University
Journal of General Plant Pathology | Year: 2010

We assessed the geographic distribution, biovar, phylotype, DNA fingerprints (rep-PCR), and/or endoglucanase sequence of potato bacterial wilt pathogen, Ralstonia solanacearum (Rs), in Japan. Rs has been isolated from potato fields in southwestern, warm, temperate regions. Of the 188 isolates, 74 belonged to biovar N2 (39%), 44 to biovar 3 (24%), and 70 to biovar 4 (37%). Biovars N2 and 4 strains were widely distributed, from northern (Hokkaido) to southern (Okinawa) Japan. Based on the results of multiplex-PCR analysis, every potato strains belonged to either phylotype I or IV. Phylotype I comprised both biovars 3 and 4 strains. On the other hand, phylotype IV included biovar N2 strains. None of the strains belonged to phylotype II or III or biovar 1 or 2. Phylogenetic analysis based on DNA fingerprints and endoglucanase gene sequences clarified the genetic diversity of the Japanese potato strains and the close genetic relationship between the Japanese strains and the Asian strains in phylotypes I and IV. © 2010 The Phytopathological Society of Japan and Springer.


Suga Y.,Nagasaki Agricultural and Forestry Technical Development Center | Suga Y.,Kyushu University | Horita M.,Japan National Institute for Agro - Environmental Sciences | Umekita M.,Kyushu University | And 2 more authors.
Journal of General Plant Pathology | Year: 2013

Ralstonia solanacearum (Rs) strains in phylotypes I and IV isolated from potato in Japan were investigated for pathogenicity on potato, tomato, eggplant, Solanumintegrifolium, tobacco, groundnut, and pumpkin. The strains were divided into 17 types based on differences in their pathogenicity on the tested plants. Particularly, the pathogenicity of most phylotype I strains on eggplant was distinctly different from that of the phylotype IV strains. When nine potato varieties (included two breeding lines) were inoculated with several Rs strains, phylotype IV strains were highly virulent on the breeding lines that are regarded as resistant to phylotype I strains. © 2013 The Phytopathological Society of Japan and Springer Japan.


Omar M.,Nagasaki University | Matsuo Y.,Nagasaki University | Maeda H.,Nagasaki Agricultural and Forestry Technical Development Center | Saito Y.,Nagasaki University | Tanaka T.,Nagasaki University
Organic Letters | Year: 2014

Two unusual ellagitannin metabolites, quercusnins A (3) and B (4), have been isolated from the sapwood of Quercus crispula, and their structures determined by spectroscopic methods, as well as DFT calculations of 1H and 13C NMR chemical shifts of the possible four diastereomers. Treatment of the major ellagitannin species, vescalagin, with Shiitake mushroom (Lentinula edodes) gave 3, which indicated that these unique ellagitannins were the fungal metabolites of ellagitannins. © 2014 American Chemical Society.


Miyazaki T.,Nagasaki Agricultural and Forestry Technical Development Center | Okayasu T.,Kyushu University | Inoue E.,Kyushu University
American Society of Agricultural and Biological Engineers Annual International Meeting 2011, ASABE 2011 | Year: 2011

Soil compaction is one of the major subjects in a well-mechanized modern agricultural system. In order to reduce the risk of soil compaction, the mechanical impacts to topsoil and subsoil during agricultural machinery operations have to be controlled. Soil compaction test using an off-road vehicle was conducted in the reclamation farmland at Isahaya bay, Nagasaki, Japan. The farmland was formed by sedimentary heavy clayey soil having high water-holding capacity and strong cohesion. In all the tests, the penetration resistance (cone index) of the soil and the bulk density before and after the vehicle running was measured in the field. The detail of soil mechanical properties was obtained from typical soil tests such as the standard compression, oedometer and triaxial compression test, respectively. On the other hand, an unconventional elastoplastic constitutive model for soils having the structure and cohesion was proposed by the superloading-yield/subloading surface model with Hashiguchi and Mase concept (2007). The validity of the model was evaluated by comparisons with the test data mentioned above.


Mori K.,Nagasaki Agricultural and Forestry Technical Development Center | Mori K.,Kobe University | Sakamoto Y.,Nagasaki Agricultural and Forestry Technical Development Center | Mukojima N.,Nagasaki Agricultural and Forestry Technical Development Center | And 4 more authors.
Euphytica | Year: 2011

Multiplex PCR is practically a reasonable choice for molecular marker-assisted selection in potato breeding. We had developed and were using a multiplex PCR method for selection of resistance genes to cyst nematode (H1), Potato virus X (Rx1) and late blight (R1 and R2). Since then, more reliable and tightly linked markers for H1 and R2, and a new marker for resistance to Potato virus Y (Rychc) were developed. In this article, all these superior markers, including a positive marker to eliminate PCR-failed samples, were incorporated into one multiplex PCR assay. Using the newly developed multiplex PCR technique, five plants potentially harboring all five resistance genes were selected from 96 hybrid plants approximately 5 h after DNA extraction, which is a third of the operation time compared with separate PCR reactions for each marker. © 2011 Springer Science+Business Media B.V.


Mori K.,Nagasaki Agricultural and Forestry Technical Development Center | Mori K.,Kobe University | Mukojima N.,Nagasaki Agricultural and Forestry Technical Development Center | Nakao T.,Nagasaki Agricultural and Forestry Technical Development Center | And 9 more authors.
American Journal of Potato Research | Year: 2012

Saikai 35 was bred from a cross between TD0101 as the female, which was created by chromosome-doubling of a good-tasting and bacterial wilt resistant diploid variety, Inca-no-mezame, and Sakurafubuki as the male, the latter of which has H1 and Ry chc genes showing resistance to potato cyst nematode (PCN) and Potato virus Y (PVY), respectively. All favorable traits were combined into Saikai 35, although marketable yield in the spring cropping was 20.4-21.0% lower than those of major double-cropping varieties. Saikai 35 is particularly useful for having Solanum phureja-derived cytoplasm (S/ε), which resulted in high male and female fertility. In addition, sets of very tightly linked DNA markers sandwiching H1 and Ry chc are available. Therefore, Saikai 35 is being released as a breeding line, which can confer efficiently PCN and PVY resistance genes. © 2011 Potato Association of America.


Omar M.,Nagasaki University | Omar M.,Al - Azhar University of Egypt | Matsuo Y.,Nagasaki University | Maeda H.,Nagasaki Agricultural and Forestry Technical Development Center | And 2 more authors.
Phytochemistry Letters | Year: 2013

Two novel glycosides, 4,5-dimethoxy-3-hydroxyphenol 1-O-β-(6′-O- galloyl)-glucopyranoside (1) and (+)-2α-O-galloyl lyoniresinol 3α-O-β-D-xylopyranoside (2), as well as a novel ellagitannin named epiquisqualin B (3), were isolated from sapwood of Quercus mongolica var. crispula along with 19 known phenolic compounds. The structures of the novel compounds were elucidated on the basis of chemical and spectroscopic investigation. Compound 2 is the first example of a lignan galloyl ester, and 3 is the oxidation product of vescalagin, which is the major ellagitannin of this plant. © 2013 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved.

Loading Nagasaki Agricultural and Forestry Technical Development Center collaborators
Loading Nagasaki Agricultural and Forestry Technical Development Center collaborators