Nagano Agricultural Experiment Station

Nagano-shi, Japan

Nagano Agricultural Experiment Station

Nagano-shi, Japan
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Abe T.,Japan Institute for Environmental Sciences | Ito M.,Akita | Takahashi R.,Akita | Honma T.,Niigata Agricultural Research Institute | And 5 more authors.
Soil Science and Plant Nutrition | Year: 2017

Previously, we showed that qCdp7, an allele identified in the high-Cd-accumulating indica rice variety ‘Jarjan,’ is associated with effective phytoextraction of Cd from paddy soils. However, ‘Jarjan’ may not be practical for phytoextraction because it is susceptible to seed shattering and culm lodging, which are unfavorable traits for mechanical rice harvesting. In this study, to develop a practical rice line for phytoextraction, we introduced the qCdp7 allele into ‘Tachisugata,’ a rice variety with a nonshattering habit and lodging resistance, to produce a new high-Cd-accumulating rice line designated ‘TJTT8.’ This line inherited high-Cd accumulation and brown pericarps from ‘Jarjan’ and a nonshattering habit and lodging resistance from ‘Tachisugata;’ all of these traits are necessary for rice intended for Cd phytoextraction in Japan. Backcross inbred lines (BILs) were produced by two backcrosses to ‘Tachisugata’ after a cross between ‘Jarjan’ and ‘Tachisugata.’ ‘TJTT8’ was selected from the BILs by means of marker-assisted selection and phenotypic evaluation. When ‘TJTT8,’ the parents, and ‘Cho-ko-koku’ which is a high-Cd-accumulating indica variety were cultivated in Cd-contaminated paddy fields in four locations in Japan, ‘TJTT8’ exhibited lodging resistance and shattering resistance that were higher than those of ‘Jarjan’ and ‘Cho-ko-koku’ and equivalent to those of ‘Tachisugata.’ ‘TJTT8’ accumulated Cd in the aerial parts of the plants at concentrations ranging from 6.5 to 22.7 mg m−2: it showed significantly higher Cd accumulation than ‘Tachisugata’ and was equivalent to ‘Jarjan’ and slightly superior to ‘Cho-ko-koku.’ Soil Cd concentration was estimated to have been reduced by 8.7–33.6% based on the amount of Cd accumulation in the aerial parts of the plants. Thus, we succeeded in using the qCdp7 allele to produce a practical rice line for Cd phytoextraction by improving several agronomic traits for compatibility with Japanese cultivation systems. © 2017 Japanese Society of Soil Science and Plant Nutrition


Kubo K.,Japan National Agriculture and Food Research Organization | Fujita M.,Japan National Agriculture and Food Research Organization | Kawada N.,Japan National Agriculture and Food Research Organization | Nakajima T.,Japan National Agriculture and Food Research Organization | And 5 more authors.
Journal of Phytopathology | Year: 2013

Fusarium head blight (FHB) remains a serious problem due to yield loss and mycotoxin accumulation in wheat production worldwide. We previously reported that the closed-flowering (no anther extrusion) characteristic was effective for increasing resistance to FHB infection. In this study, we investigated the relationships between the degree of anther extrusion (AE) and FHB damage using double haploid lines (DHLs), derived from F1 plants from crosses between closed-flowering and opened-flowering varieties. These DHLs exhibited various degrees of AE, and the degree of AE was significantly different among DHLs, regardless of the year and environment (pot- or field-grown). FHB severity was the lowest in closed-flowering DHLs, and DHLs with partially extruded anthers showed significantly higher FHB symptoms than those with closed-flowering phenotypes. In general, DHLs with partially extruded anthers also had relatively severe FHB symptoms compared with those exhibiting full anther extrusion. FHB severity was significantly correlated with Fusarium-damaged kernels and deoxynivalenol concentration. The results of this study showed that partially extruded anthers were considered to be a source of FHB infection. The closed-flowering phenotype improved resistance to FHB infection. Meanwhile, phenotypes with rapid anther extrusion and ejection also could contribute to the avoidance of FHB infection. © 2012 Blackwell Verlag GmbH.


Ishikawa G.,Japan National Agriculture and Food Research Organization | Nakamura K.,Japan National Agriculture and Food Research Organization | Ito H.,Japan National Agriculture and Food Research Organization | Saito M.,Japan National Agriculture and Food Research Organization | And 8 more authors.
PLoS ONE | Year: 2014

The winter wheat variety Kitahonami shows a superior flour yield in comparison to other Japanese soft wheat varieties. To map the quantitative trait loci (QTL) associated with this trait, association mapping was performed using a panel of lines from Kitahonami's pedigree, along with leading Japanese varieties and advanced breeding lines. Using a mixed linear model corrected for kernel types and familial relatedness, 62 marker-trait associations for flour yield were identified and classified into 21 QTLs. In eighteen of these, Kitahonami alleles showed positive effects. Pedigree analysis demonstrated that a continuous pyramiding of QTLs had occurred throughout the breeding history of Kitahonami. Linkage analyses using three sets of doubled haploid populations from crosses in which Kitahonami was used as a parent were performed, leading to the validation of five of the eight QTLs tested. Among these, QTLs on chromosomes 3B and 7A showed highly significant and consistent effects across the three populations. This study shows that pedigree-based association mapping using breeding materials can be a useful method for QTL identification at the early stages of breeding programs. © 2014 Ishikawa et al.


PubMed | Japan National Institute of Agrobiological Science, Hokkaido Research Organization, Japan National Agriculture and Food Research Organization and Nagano Agricultural Experiment Station
Type: Journal Article | Journal: PloS one | Year: 2014

The winter wheat variety Kitahonami shows a superior flour yield in comparison to other Japanese soft wheat varieties. To map the quantitative trait loci (QTL) associated with this trait, association mapping was performed using a panel of lines from Kitahonamis pedigree, along with leading Japanese varieties and advanced breeding lines. Using a mixed linear model corrected for kernel types and familial relatedness, 62 marker-trait associations for flour yield were identified and classified into 21 QTLs. In eighteen of these, Kitahonami alleles showed positive effects. Pedigree analysis demonstrated that a continuous pyramiding of QTLs had occurred throughout the breeding history of Kitahonami. Linkage analyses using three sets of doubled haploid populations from crosses in which Kitahonami was used as a parent were performed, leading to the validation of five of the eight QTLs tested. Among these, QTLs on chromosomes 3B and 7A showed highly significant and consistent effects across the three populations. This study shows that pedigree-based association mapping using breeding materials can be a useful method for QTL identification at the early stages of breeding programs.


Noguchi T.,Nagano Agricultural Experiment Station
Japanese Journal of Applied Entomology and Zoology | Year: 2014

In the laboratory, the adult rice leaf bug, Trigonotylus caelestialium Kirkaldy, most preferred 375nm light-emitting diodes LED among lights of four wavelengths ranging from 375 to 450nm with constant light intensity. Under a rated output current condition, short-wavelength range LEDs 375nm, 400nm attracted more bugs than longwavelength range LEDs 505-600nm. In the field, the prototype LED 375 or 400nm trap caught few adult bugs of the overwintering generation in the spring April to mid-June, while it caught adult bugs continuously during the summer late June to September. Many adult bugs of the presumed second generation, which is considered to be especially related to pecky rice damage, were caught in the trap from late July to mid-August. Thus, LED traps might be useful tools to monitor T. carestialium under field conditions.

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