N Shankaran Nair Research Institute For Nanotechnology And Bionanotechnology

Ambernath, India

N Shankaran Nair Research Institute For Nanotechnology And Bionanotechnology

Ambernath, India
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Pandey S.,N Shankaran Nair Research Institute For Nanotechnology And Bionanotechnology | Oza G.,N Shankaran Nair Research Institute For Nanotechnology And Bionanotechnology | Mewada A.,N Shankaran Nair Research Institute For Nanotechnology And Bionanotechnology | Shah R.,N Shankaran Nair Research Institute For Nanotechnology And Bionanotechnology | And 2 more authors.
Journal of Materials Chemistry B | Year: 2013

Azadirachta indica as a biological sink for fabrication of gold nanoparticles (GNPs) and its applications in efficient delivery of doxorubicin (DOX) are presented here. Sucrose density gradient centrifugation was used to isolate the spherical GNPs of <50 nm from the mixture (containing both spherical and non-spherical) of nanoparticles synthesized using leaves of A. indica at inherent pH (6.14). The stability of GNPs due to the biological capping agents was scrutinised by measuring the flocculation parameter which was found to be in the range of 0-0.65. On the surface of these capped GNPs, doxorubicin was attached along with activated folic acid (FA) as navigational molecules for targeted drug delivery. Attachments were verified using FTIR which confirmed the formation of non-covalent interactions. The GNPs-FA-DOX complex was found be non-toxic for normal cells and considerably toxic for HeLa cells. The drug loading capacity of the GNPs was found to 93%. Doxorubicin release kinetics using GNPs followed 1st order at pH 5.3 which is ideal for solid tumor targeting. © 2013 The Royal Society of Chemistry.


PubMed | N Shankaran Nair Research Institute for Nanotechnology and Bionanotechnology
Type: | Journal: Colloids and surfaces. B, Biointerfaces | Year: 2013

We are presenting facile bio-fabrication of extremely stable gold nanoparticles (GNPs) using medicinal plant Azadirachta indica (commonly called Neem) and its comparison with most commonly used glutathione (GSH) protected GNPs in terms of stability under physiological conditions, seperation using density gradient centrifugation and aggregation properties in the solution. There was dual peak at 536 and 662 nm indicating the presence of non-spherical GNPs including triangles, rods and hexagons in case of A. indica mediated GNPs unlike citrate stabilized GNPs which exhibited single sharp peak. Spherical GNPs were separated from the consortium of uniquely shaped nanoparticles bio-fabricated using A. indica leaf extract using sucrose density gradient centrifugation (SDGC).To comprehend the anti-agglomeration potentials of A. indica leaf mediated GNPs and GSH-GNPs under physiological conditions, flocculation parameters (FP) were calculated and found to be least for A. indica leaf mediated GNPs, indicating their exceptional stability.

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