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Ghita M.A.,University of Bucharest | Caruntu C.,University of Bucharest | Caruntu C.,Prof N Paulescu National Institute Of Diabetes | Rosca A.E.,University of Bucharest | And 10 more authors.
Oncology Letters | Year: 2016

Superficial basal cell carcinoma (sBCC) is the second most frequent histological type of basal cell carcinoma (BCC), usually requiring a skin biopsy to confirm the diagnosis. It usually appears on the upper trunk and shoulders as erythema­tous and squamous lesions. Although it has a slow growth and seldom metastasizes, early diagnosis and management are of crucial importance in preventing local invasion and subsequent disfigurement. Dermoscopy is nowadays an indispensable tool for the dermatologist when evaluating skin tumors. Reflectance confocal microscopy (RCM) is a novel imaging technique that allows the non‑invasive, in vivo quasi‑microscopic morpho­logical and dynamic assessment of superficial skin tumors. Moreover, it offers the advantage of performing infinite repeatable determinations to monitor disease progression and non-surgical treatment for sBCC. Herein, we present three lesions of sBCC evaluated using in vivo and non‑invasive imaging techniques, emphasizing the usefulness of combining RCM with dermoscopy for increasing the diagnostic accuracy of sBCC. © 2016, Spandidos Publications. All rights reserved.


PubMed | Prof N Paulescu National Institute Of Diabetes, Carol Davila University of Medicine and Pharmacy, University of Medicine and Pharmacy of Craiova, Carol Davila Central Military Emergency Hospital and 2 more.
Type: Journal Article | Journal: Oncology letters | Year: 2016

Superficial basal cell carcinoma (sBCC) is the second most frequent histological type of basal cell carcinoma (BCC), usually requiring a skin biopsy to confirm the diagnosis. It usually appears on the upper trunk and shoulders as erythematous and squamous lesions. Although it has a slow growth and seldom metastasizes, early diagnosis and management are of crucial importance in preventing local invasion and subsequent disfigurement. Dermoscopy is nowadays an indispensable tool for the dermatologist when evaluating skin tumors. Reflectance confocal microscopy (RCM) is a novel imaging technique that allows the non-invasive,


Neagu M.,Victor Babes National Institute of Pathology | Neagu M.,University of Bucharest | Caruntu C.,Carol Davila University of Medicine and Pharmacy | Caruntu C.,Prof N Paulescu National Institute Of Diabetes | And 5 more authors.
Oncology Reports | Year: 2016

Skin cancer is one of the most common malignancies affecting humans worldwide, and its incidence is rapidly increasing. The study of skin carcinogenesis is of major interest for both scientific research and clinical practice and the use of in vivo systems may facilitate the investigation of early alterations in the skin and of the mechanisms involved, and may also lead to the development of novel therapeutic strategies for skin cancer. This review outlines several aspects regarding the skin toxicity testing domain in mouse models of chemically induced skin carcinogenesis. There are important strain differences in view of the histological type, development and clinical evolution of the skin tumor, differences reported decades ago and confirmed by our hands-on experience. Using mouse models in preclinical testing is important due to the fact that, at the molecular level, common mechanisms with human cutaneous tumorigenesis are depicted. These animal models resemble human skin cancer development, in that genetic changes caused by carcinogens and pro-inflammatory cytokines, and simultaneous inflammation sustained by pro-inflammatory cytokines and chemokines favor tumor progression. Drugs and environmental conditions can be tested using these animal models. keeping in mind the differences between human and rodent skin physiology.


Rusu E.,Prof N Paulescu National Institute Of Diabetes | Rusu E.,Carol Davila University of Medicine and Pharmacy | Rusu E.,Healthy Nutrition Foundation | Jinga M.,Carol Davila University of Medicine and Pharmacy | And 23 more authors.
Nutrition Journal | Year: 2013

Background: In patients with chronic hepatitis C (CHC), obesity is involved in the pathogenesis of insulin resistance, fatty liver disease and progression of fibrosis. The objective of this study was to compare a normoglucidic low-calorie diet (NGLCD) with a low-fat diet (LFD) among participants with CHC. Aimed to measure the impact of dietary changes in reduction of insulin resistance, obesity but also in steatosis and fibrosis. Methods. Randomized, controlled trial in three medical centers with assessments at baseline, 6 months and 12 months. Participants were patients over 35 years with chronic hepatitis C (n = 120) with BMI over 25 kg/m§ssup§2§esup§. We evaluated the effects of NGLCD vs. LFD in weight management and metabolic improvement. The primary endpoint was to measure the impact of dietary changes through nutritional intervention in reversibility of insulin resistance, obesity, steatosis, and fibrosis. We performed anthropometric measurements, fasting glucose profile, serum lipids, liver profile, blood count at baseline, 6 and 12 months. Steatosis was evaluated using ultrasonographic criteria. Liver fibrosis was non-invasively assessed. Results: After 6 and 12 months of intervention, both groups had a significant decrease in caloric consumption. At 6 months, weight loss was greater in the NGLCD group (-5.02 ± 3.43 kg vs. -4.1 ± 2.6 kg; p = 0.002) compared to the LFD group. At 1-year, however, weight loss was similar in both groups (-3.9 ± 3.3 kg vs. -3.1 ± 2.6 kg; p = 0.139). At 12 months, fasting plasma glucose, fasting plasma insulin, and HOMA-IR had significant improvements in both groups. With both diets aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transpeptidase (GGT) decreased with significant differences; also there were significant improvements in AST/ALT ratio, Forns fibrosis index. The two diets were associated with reduction of both the prevalence and the severity of steatosis (all p < 0.001). At 12 months, total cholesterol, HDL-cholesterol, triglycerides improved in both groups (all p < 0.05). Conclusions: The present study establishes the benefits of low-calorie diet and low-fat diet in management of patients with hepatitis C regarding improvement of insulin resistance, steatosis and also fibrosis.Overweight or obese patients with CHC undergoing a lifestyle intervention (specific dietary intervention and physical activity) for 1-year had significant improvements in body weight, lipid and hepatic profile. Trial registration. PNCI2-3343/41008/2007. © 2013 Rusu et al.; licensee BioMed Central Ltd.


Rusu E.,Prof N Paulescu National Institute Of Diabetes | Rusu E.,Healthy Nutrition Foundation | Rusu E.,Carol Davila University of Medicine and Pharmacy | Radulian G.,Prof N Paulescu National Institute Of Diabetes | And 15 more authors.
Romanian Journal of Diabetes, Nutrition and Metabolic Diseases | Year: 2011

Aims: The aims of this study was to indentify independent predictive factors of liver steatosis in patients with hepatitis C and to examine the correlations between ultrasound-diagnosed HS and anthropometric, clinical, biochemical and sonographic measurements. Materials and methods: We included 173 patients with CHC. HS has a high prevalence in diabetes mellitus patients, and we considered patients with CHC and diabetes as a separate subgroup for analysis: 113 patients (54 men/59 women) with hepatitis C - group A and 60 patients (28 men/32 women) with hepatitis C and T2DM - group B. We performed anthropometric measurements (weight, height, BMI (body mass index), as well as laboratory analysis and ultrasonography. All patients signed an informed consent before inclusion in the study. Results: In all patients with CHC, in univariate analysis triglycerides, hypertension, MetS, HOMA-IR >2, T2DM, AST/ALT ratio >1, and BMI >30kg/m2 was associated with HS. Logistic regression analysis revealed five factors independently related with ultrasound-diagnosed HS: the triglyceride levels, hypertension, the BMI, AST/ALT ratio, and T2DM. Conclusions: This study revealed a high prevalence of ultrasound-diagnosed HS in patients CHC and CHC and T2DM. Identification of risk factor for HS has therapeutic importance, because targeted therapies might prevent the progression of HS to fibrosis and cirrhosis.


PubMed | Colentina Clinical Hospital, Prof N Paulescu National Institute Of Diabetes, Carol Davila University of Medicine and Pharmacy, University of Crete and Victor Babes National Institute of Pathology
Type: Journal Article | Journal: Oncology reports | Year: 2016

Skin cancer is one of the most common malignancies affecting humans worldwide, and its incidence is rapidly increasing. The study of skin carcinogenesis is of major interest for both scientific research and clinical practice and the use of in vivo systems may facilitate the investigation of early alterations in the skin and of the mechanisms involved, and may also lead to the development of novel therapeutic strategies for skin cancer. This review outlines several aspects regarding the skin toxicity testing domain in mouse models of chemically induced skin carcinogenesis. There are important strain differences in view of the histological type, development and clinical evolution of the skin tumor, differences reported decades ago and confirmed by our handson experience. Using mouse models in preclinical testing is important due to the fact that, at the molecular level, common mechanisms with human cutaneous tumorigenesis are depicted. These animal models resemble human skin cancer development, in that genetic changes caused by carcinogens and proinflammatory cytokines, and simultaneous inflammation sustained by proinflammatory cytokines and chemokines favor tumor progression. Drugs and environmental conditions can be tested using these animal models. keeping in mind the differences between human and rodent skin physiology.


In patients with chronic hepatitis C (CHC), obesity is involved in the pathogenesis of insulin resistance, fatty liver disease and progression of fibrosis. The objective of this study was to compare a normoglucidic low-calorie diet (NGLCD) with a low-fat diet (LFD) among participants with CHC. Aimed to measure the impact of dietary changes in reduction of insulin resistance, obesity but also in steatosis and fibrosis.Randomized, controlled trial in three medical centers with assessments at baseline, 6 months and 12 months. Participants were patients over 35 years with chronic hepatitis C (n=120) with BMI over 25 kg/m. We evaluated the effects of NGLCD vs. LFD in weight management and metabolic improvement. The primary endpoint was to measure the impact of dietary changes through nutritional intervention in reversibility of insulin resistance, obesity, steatosis, and fibrosis. We performed anthropometric measurements, fasting glucose profile, serum lipids, liver profile, blood count at baseline, 6 and 12 months. Steatosis was evaluated using ultrasonographic criteria. Liver fibrosis was non-invasively assessed.After 6 and 12 months of intervention, both groups had a significant decrease in caloric consumption. At 6 months, weight loss was greater in the NGLCD group (-5.023.43 kg vs. -4.12.6 kg; p=0.002) compared to the LFD group. At 1-year, however, weight loss was similar in both groups (-3.93.3 kg vs. -3.12.6 kg; p=0.139). At 12 months, fasting plasma glucose, fasting plasma insulin, and HOMA-IR had significant improvements in both groups. With both diets aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transpeptidase (GGT) decreased with significant differences; also there were significant improvements in AST/ALT ratio, Forns fibrosis index. The two diets were associated with reduction of both the prevalence and the severity of steatosis (all p<0.001). At 12 months, total cholesterol, HDL-cholesterol, triglycerides improved in both groups (all p<0.05).The present study establishes the benefits of low-calorie diet and low-fat diet in management of patients with hepatitis C regarding improvement of insulin resistance, steatosis and also fibrosis.Overweight or obese patients with CHC undergoing a lifestyle intervention (specific dietary intervention and physical activity) for 1-year had significant improvements in body weight, lipid and hepatic profile.PNCI2-3343/41008/2007.

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