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Invergowrie, United Kingdom

Cerekovic N.,University of Aarhus | Jarret D.,Mylnefield Research Services Ltd | Pagter M.,University of Aarhus | Pagter M.,Max Planck Institute of Molecular Plant Physiology | And 5 more authors.
European Journal of Horticultural Science | Year: 2015

This study provides genome expression analyses from the blackcurrant cultivar ‘Ben Gairn’ after five days of drought stress. RNA Sequencing (RNA-Seq) data was utilized to generate a non-redundant set of 40,225 predicted transcripts used to design a custom Ribes microarray. A set of 2,115 differentially expressed genes were identified during drought treatment; 429 of these genes were up-regulated, with 263 showing homology to unique Arabidop-sis thaliana (At) accessions, and 1,686 genes were down-regulated, with 675 unique At numbers. The Arabidopsis homologs were analysed for enrichment of GO (gene ontology) terms using the Term Enrichment Tool. This showed a number of GO terms highly enriched in the drought up-regulated and down-regulated gene lists in GO categories associated with molecular function, biological process and cellular component. The identification of several hormone metabolism, cell wall, cell cycle, and transcription factor genes indicated that they could play an important role in the drought stress tolerance response. The results provide relevant information for focusing future studies with the aim to develop drought tolerant cultivars for sustainable production. © ISHS 2015.

Clark K.E.,James Hutton Institute | Clark K.E.,University of Sussex | Hartley S.E.,University of Sussex | Hartley S.E.,University of York | And 8 more authors.
Crop Protection | Year: 2012

Black vine weevil (Otiorhynchus sulcatus) is regarded as a damaging pest of horticultural crops, yet empirical data about its population dynamics and effects on crop yield are largely lacking. This paper reports a four year (2007-2010) field study that simulated colonisation by O. sulcatus in a red raspberry (Rubus idaeus) plantation comprising two cultivars (Glen Ample and Glen Rosa). The study tested whether adult O. sulcatus abundance was affected by cultivar or original egg distribution. Total population estimates were also made using mark and recapture methods. Finally, the effects of O. sulcatus on plant growth and berry yield were quantified. O. sulcatus abundance was significantly higher on Glen Ample than Glen Rosa, with the difference becoming even greater in 2009 compared to 2008. The abundance of O. sulcatus adults on particular plants was not related to original egg density. Mark and recapture based estimates suggested that the overall O. sulcatus population reached 3500 in 2008 and 44,100 in 2009. O. sulcatus abundance on plants was negatively related to plant height. Comparing plants with greatest and lowest O. sulcatus burdens showed that the heavily infested plants had lower shoot and root biomass in both Glen Ample (62% and 60%, respectively) and Glen Rosa (50% and 12%, respectively), significantly smaller berries (5.1g and 2.8g, respectively, in Glen Ample and 3.3g and 2.7g, respectively, in Glen Rosa) and smaller yields (2.93kg and 0.99kg in Glen Ample and 2.80kg-1.71kg in Glen Rosa, respectively). While cane fruits like raspberry were thought to tolerate O. sulcatus attack due to extensive root systems, this study suggests that damage can be extensive, particularly on cultivars such as Glen Ample. © 2011 Elsevier Ltd.

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