Brennan R.,James Hutton Institute |
Russell J.,James Hutton Institute |
Jorgensen L.,James Hutton Institute |
Jarret D.,Mylnefield Research Services |
Hackett C.,Biomathematics and Statistics Scotland
Acta Horticulturae | Year: 2012
The deployment of marker-assisted breeding strategies in blackcurrant has started with the use of a PCR-based marker for gall mite resistance. This is now a routine part of the selection process within the breeding programmes at the James Hutton Institute, and further work is in progress to develop similar approaches for other more complex traits. Whilst the need for accurate field- and glasshouse-based phenotyping has never been more vital, the integration of enhanced phenotyping with he development of significant genomics resources offers an important opportunity for the improvement of breeding efficiency in blackcurrant.
Prashar A.,James Hutton Institute |
Hornyik C.,James Hutton Institute |
Young V.,Mylnefield Research Services |
McLean K.,James Hutton Institute |
And 4 more authors.
Theoretical and Applied Genetics | Year: 2014
Key message Generation of a dense SNP-based linkage map of a diploid potato population and identification of major QTLs for tuber shape and eye depth on chromosomes 2 and 10. This paper reports the construction of a genetic map of a highly heterozygous full-sib diploid potato population (06H1) based on the use of a set of 8,303 single nucleotide polymorphism (SNP) markers. The map contains 1,355 distinct loci and 2,157 SNPs, 802 of which co-segregate with other markers. We find high levels of collinearity between the 12 chromosomal maps with a recently improved version of the potato genome assembly, with the expected genetic clustering in centromeric regions. The linkage maps are used in combination with highly detailed phenotypic assessments conducted over two growing seasons to perform quantitative trait loci analysis of two important potato traits, tuber shape and eye depth. The major loci segregating for tuber shape in 06H1 map to loci on chromosomes 2 and 10, with smaller effects mapping to three other chromosomes. A major locus for tuber eye depth co-locates with the tuber shape locus on chromosome 10. To assess when tuber shape is established in the developing tuber, we have performed staged observations of tuber formation. Our observations suggest that tuber shape is determined very early in tuber development. © 2014 Springer-Verlag Berlin Heidelberg.
Meier-Augenstein W.,James Hutton Institute |
Kemp H.F.,James Hutton Institute |
Hardie S.M.L.,Mylnefield Research Services
Food Chemistry | Year: 2012
Neat Scottish whiskies and the local source water used for and during whisky production have been analysed for their 2H and 18O isotopic composition by High Temperature Conversion-Isotope Ratio Mass Spectrometry (TC/EA-IRMS). With coefficients of determination R 2 for δ 2H and δ 18O of 0.71 and 0.88, respectively, measured δ 2H and δ 18O values for water used in the production of Scottish whisky were found to be well correlated with the δ 2H and δ 18O values observed for the corresponding whiskies. By plotting bulk 2H against bulk 18O isotope abundance data of authentic whisky and counterfeit whisky samples it was possible to discriminate between samples of authentic whisky and samples of counterfeit whisky. © 2012 Elsevier Ltd. All rights reserved.