Laboratory, India
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Verma R.,Indian Veterinary Research Institute | Verma R.,Mycobacteria Laboratory | Sena D.S.,National Research Center on Camel | Sharma N.,National Research Center on Camel | And 4 more authors.
Indian Journal of Animal Sciences | Year: 2011

An adult male camel was diagnosed with tuberculosis (TB). It exhibited typical TB lesions in the lungs, liver and spleen. The histopathological examination of sections of tissues revealed presence of acid-fast bacilli. Mycobacteria were isolated from the camel's lung and were identified as the member of Mycobacterium tuberculosis complex (MTBC) subsequently confirmed as M. bovis by biochemical tests and multiplex PCR where 445 bp band indicative of MTBC and 823 bp band indicative of M. bovis was observed.

Ramane S.,Mycobacteria Laboratory | Verma R.,Center for Animal Disease Research and Diagnosis | Mondal T.,Mycobacteria Laboratory | Upmanyu V.,Indian Veterinary Research Institute
Journal of Pure and Applied Microbiology | Year: 2014

Mycobacterium bovis 3/86 strain isolated from cattle was characterized based on RD region encoded Mb3904, Mb3905 and Mb2002c gene sequences. PCR was performed to amplify Mb3904, Mb390S and Mb2002c genes. Restriction enzymes digested amplified geneswere cloned in compatible pET vector and sequenced with vector specific primers. The sequenced genes and its deduced amino acid sequences were compared with the published sequences of reference strains. The sequences of the Mb3904, Mb3905 and Mb2002c genes share 99.6 to 100% nucleotide homology and 99.5 to 100% deduced protein sequence homology for all studied genes with published reference mycobacterial strains indicating their conserved nature.

Kidangan A.,Indian Veterinary Research Institute | Verma R.,Indian Veterinary Research Institute | Verma R.,Mycobacteria Laboratory
Indian Journal of Animal Sciences | Year: 2014

The usefulness of polymerase chain reaction-single stranded confirmation polymorphism (PCR-SSCP) for determination of rifampicin and isoniazid resistance in Mycobacterium tuberculosis and M. bovis cultures from human and animal origin was investigated. Mycobacteria (81) in the study included, viz. 12 MDR-TB samples, 35 sputum samples, 3 lung and lymphnode tissues from bovines and 11 M. tuberculosis and 18 M. bovis culture, M. tuberculosis H37Rv and M. bovis BCG strain). All the mycobacterial cultures were characterized on growth characteristics, biochemical test pattern, MTB complex specific IS6110 PCR and species specific 12.7 kb multiplex PCR. PCR-SSCP was used to determine resistance against rifiampin by targeting rpoB gene (305 bp) and isoniazid by targeting katG (237 bp) and inhA (261 bp). Rifampicin resistance was detected by PCR-SSCP in 1 out of 12 MDR-TB samples (8.3%), while isoniazid resistance was detected in 66.7% of MDR-TB samples using PCR-SSCP of katG and 75% of MDR-TB samples using inhA SSCP analysis.

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