Mybasol srl

Alessandria del Carretto, Italy

Mybasol srl

Alessandria del Carretto, Italy
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Gamalero E.,University of Piemonte Orientale | Marzachi C.,CNR Institute of Plant virology | Galetto L.,CNR Institute of Plant virology | Veratti F.,CNR Institute of Plant virology | And 8 more authors.
Plant Biosystems | Year: 2016

Flavescence dorée is an epidemic yellows disease of grapevine, caused by a phytoplasma (FDP), for which there is currently no cure. We assessed whether the endophyte Pseudomonas migulae 8R6, able to synthesize 1-aminocyclopropane-1-carboxylate (ACC) deaminase, can limit the phytoplasma-induced damages in periwinkle, a model plant hosting FDP. Plant protection induced by 8R6 and its mutant, impaired in ACC deaminase synthesis, was compared. Fifteen plants per treatment were used; FD infection was transmitted by grafting. Evaluation of symptoms was performed every 4 days for 40 days. The presence and the amount of FDP were assessed by nested PCR and qPCR, respectively. Images of phytoplasma inside the infected plants were obtained by transmission electron microscopy. The strain 8R6 significantly reduced the number of symptomatic plants (53% vs 93%). While the density of FDP inside the leaves was unaffected by the bacterial strains, the FDP titre was under the quantification threshold in 38% of the plants inoculated with strain 8R6. Microscopical observations showed damaged FDP cells in plants inoculated with strain 8R6. The ACC deaminase activity of the endophytic bacteria P. migulae 8R6 helps the plant to regulate the level of the stress-related hormone ethylene, leading to significantly improved resistance to phytoplasma infection. © 2016 Società Botanica Italiana


Lingua G.,University of Piemonte Orientale | Copetta A.,University of Piemonte Orientale | Copetta A.,Mybasol S.r.l. | Copetta A.,Cra Unita Of Ricerca Per Le Produzioni Legnose Fuori Foresta | And 9 more authors.
Environmental Science and Pollution Research | Year: 2015

High nitrogen concentration in wastewaters requires treatments to prevent the risks of eutrophication in rivers, lakes and coastal waters. The use of constructed wetlands is one of the possible approaches to lower nitrate concentration in wastewaters. Beyond supporting the growth of the bacteria operating denitrification, plants can directly take up nitrogen. Since plant roots interact with a number of soil microorganisms, in the present work we report the monitoring of nitrate concentration in macrocosms with four different levels of added nitrate (0, 30, 60 and 90 mg l−1), using Phragmites australis, inoculated with bacteria or arbuscular mycorrhizal fungi, to assess whether the use of such inocula could improve wastewater denitrification. Higher potassium nitrate concentration increased plant growth and inoculation with arbuscular mycorrhizal fungi or bacteria resulted in larger plants with more developed root systems. In the case of plants inoculated with arbuscular mycorrhizal fungi, a faster decrease of nitrate concentration was observed, while the N%/C% ratio of the plants of the different treatments remained similar. At 90 mg l−1 of added nitrate, only mycorrhizal plants were able to decrease nitrate concentration to the limits prescribed by the Italian law. These data suggest that mycorrhizal and microbial inoculation can be an additional tool to improve the efficiency of denitrification in the treatment of wastewaters via constructed wetlands. © 2015, Springer-Verlag Berlin Heidelberg.


Bona E.,University of Piemonte Orientale | Lingua G.,University of Piemonte Orientale | Manassero P.,University of Piemonte Orientale | Cantamessa S.,University of Piemonte Orientale | And 12 more authors.
Mycorrhiza | Year: 2014

There is increasing interest in the quality of crops because of the implications concerning health, economic revenue, and food quality. Here we tested if inoculation with a mixture of arbuscular mycorrhizal fungi (AMF) and/or two strains of plant growth-promoting bacteria (PGPB), in conditions of reduced chemical inputs, affects the quality and yield of strawberry fruits. Fruit quality was measured by concentrations of soluble sugars, various organic acids, and two vitamins (ascorbic and folic acid). Co-inoculation with the AMF and each of the two PGPB resulted in increased flower and fruit production, larger fruit size, and higher concentrations of sugars and ascorbic and folic acid in comparison with fruits of uninoculated plants. These results provide further evidence that rhizospheric microorganisms affect fruit crop quality and show that they do so even under conditions of reduced chemical fertilization and can thus be exploited for sustainable agriculture. © 2014 Springer-Verlag Berlin Heidelberg.


Bona E.,University of Piemonte Orientale | Cantamessa S.,University of Piemonte Orientale | Cantamessa S.,Mybasol srl | Massa N.,University of Piemonte Orientale | And 10 more authors.
Mycorrhiza | Year: 2016

The aim of this work was to assess the effects of plant-beneficial microorganisms (two Pseudomonas strains and a mixed mycorrhizal inoculum, alone or in combination) on the quality of tomato fruits of plants grown in the field and subjected to reduced fertilization. Pseudomonas strain 19Fv1T was newly characterized during this study. The size and quality of the fruits (concentration of sugars, organic acids and vitamin C) were assessed. The microorganisms positively affected the flower and fruit production and the concentrations of sugars and vitamins in the tomato fruits. In particular, the most important effect induced by arbuscular mycorrhizal (AM) fungi was an improvement of citric acid concentration, while bacteria positively modulated sugar production and the sweetness of the tomatoes. The novelty of the present work is the application of soil microorganisms in the field, in a real industrial tomato farm. This approach provided direct information about the application of inocula, allowed the reduction of chemical inputs and positively influenced tomato quality. © 2016 Springer-Verlag Berlin Heidelberg


Berta G.,University of Piemonte Orientale | Copetta A.,University of Piemonte Orientale | Gamalero E.,University of Piemonte Orientale | Bona E.,University of Piemonte Orientale | And 3 more authors.
Mycorrhiza | Year: 2014

Arbuscular mycorrhizal (AM) fungi and plant growth-promoting bacteria (PGPB) can increase the growth and yield of major crops, and improve the quality of fruits and leaves. However, little is known about their impact on seed composition. Plants were inoculated with AM fungi and/or the bacterial strain Pseudomonas fluorescens Pf4 and harvested after 7 months of growth in open-field conditions. Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined. Plant growth and yield were increased by inoculation with the microorganisms. Moreover, spikes and grains of inoculated plants were bigger than those produced by uninoculated plants. Regarding grain composition, the bacterial strain increased grain starch content, especially the digestible components, whereas AM fungi-enhanced protein, especially zein, content. Plant inoculation with the fluorescent pseudomonad and mycorrhizal fungi resulted in additive effects on grain composition. Overall, results showed that the bacterial strain and the AM fungi promoted maize growth cultivated in field conditions and differentially affected the grain nutritional content. Consequently, targeted plant inoculation with beneficial microorganisms can lead to commodities fulfilling consumer and industrial requirements. © 2013 Springer-Verlag Berlin Heidelberg.


Cantamessa S.,University of Piemonte Orientale | Cantamessa S.,Mybasol s.r.l. | D'agostino G.,Mybasol s.r.l. | Berta G.,University of Piemonte Orientale
Plant Biosystems | Year: 2015

Pteris vittata, a fern able to hyperaccumulate arsenic (As) in its fronds, has been object of a number of studies aimed to understand the mechanisms involved in As absorption and tolerance. This study has focused on a new mechanism, As leaching, already observed in P. vittata, but not explained, based on the possible involvement of hydathodes, not yet described in this fern; moreover, the results contained in this article will provide information on a more detailed frond anatomy of P. vittata. A combination of light and electron microscopic techniques (transmission electron microscopy and environmental scanning electron microscopy with energy dispersive X-ray) and chemical analyses (inductively coupled plasma atomic emission spectroscopy) was used. The results suggest that in phytoremediation processes under field conditions there could be the need to know the atmospheric conditions before harvesting plants, to avoid the loss of As in the environment. © 2015 Società Botanica Italiana


Lingua G.,University of Piemonte Orientale | Bona E.,University of Piemonte Orientale | Manassero P.,University of Piemonte Orientale | Marsano F.,University of Piemonte Orientale | And 10 more authors.
International Journal of Molecular Sciences | Year: 2013

Anthocyanins are a group of common phenolic compounds in plants. They are mainly detected in flowers and fruits, are believed to play different important roles such as in the attraction of animals and seed dispersal, and also in the increase of the antioxidant response in tissues directly or indirectly affected by biotic or abiotic stress factors. As a major group of secondary metabolites in plants commonly consumed as food, they are of importance in both the food industry and human nutrition. It is known that arbuscular mycorrhizal (AM) fungi can influence the plant secondary metabolic pathways such as the synthesis of essential oils in aromatic plants, of secondary metabolites in roots, and increase flavonoid concentration. Plant Growth-Promoting Bacteria (PGPB) are able to increase plant growth, improving plant nutrition and supporting plant development under natural or stressed conditions. Various studies confirmed that a number of bacterial species living on and inside the root system are beneficial for plant growth, yield and crop quality. In this work it is shown that inoculation with AM fungi and/or with selected and tested Pseudomonas strains, under conditions of reduced fertilization, increases anthocyanin concentration in the fruits of strawberry. © 2013 by the authors; licensee MDPI, Basel, Switzerland.


PubMed | Mybasol srl and University of Piemonte Orientale
Type: Journal Article | Journal: Mycorrhiza | Year: 2016

The aim of this work was to assess the effects of plant-beneficial microorganisms (two Pseudomonas strains and a mixed mycorrhizal inoculum, alone or in combination) on the quality of tomato fruits of plants grown in the field and subjected to reduced fertilization. Pseudomonas strain 19Fv1T was newly characterized during this study. The size and quality of the fruits (concentration of sugars, organic acids and vitamin C) were assessed. The microorganisms positively affected the flower and fruit production and the concentrations of sugars and vitamins in the tomato fruits. In particular, the most important effect induced by arbuscular mycorrhizal (AM) fungi was an improvement of citric acid concentration, while bacteria positively modulated sugar production and the sweetness of the tomatoes. The novelty of the present work is the application of soil microorganisms in the field, in a real industrial tomato farm. This approach provided direct information about the application of inocula, allowed the reduction of chemical inputs and positively influenced tomato quality.

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