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Karabasanavar N.S.,Mumbai Veterinary College | Singh S.P.,University of Veterinary and Animal Sciences | Umapathi V.,Indian Council of Agricultural Research | Kumar D.,Indian Veterinary Research Institute | Shebannavar S.N.,Gennova Biopharmaceutical Pvt. Ltd
Journal of Food Quality | Year: 2011

A highly species-specific polymerase chain reaction (PCR) assay was developed for the authentic identification of goat. A product of 436bp was amplified using newly designed primers against mitochondrial D-loop region. The possibility of cross-amplification was ruled out by considering as many as 25 other animal species. Suitability of the developed goat species-specific PCR assay was confirmed for in raw, cooked (60, 80 and 100C for 30min) and micro-oven-processed meat samples (n=20 each). A sensitivity of 0.1% was established for detection of adulteration and limit of detection of goat DNA was 0.1pg. This investigation presents a novel PCR assay with its newly designed primers that could be used for the authentic identification of goat species. © 2011 Wiley Periodicals, Inc..

Hagawane T.N.,Maharashtra University of Health Sciences | Gaikwad R.V.,Mumbai Veterinary College | Kshirsagar N.A.,Indian Council of Medical Research
Indian Journal of Medical Research | Year: 2016

Background & objectives: Despite advances in therapy and overall medical care, acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) management remains a problem. Hence the objective of this study was to develop a rat model that mimics human ALI/ARDS. Methods: Four groups of Wistar rats, 48 per group were treated with (i) intratracheal (IT) lipopolysaccharide (LPS) (5 mg/kg) dissolved in normal saline (NS), (ii) intravenous (iv) oleic acid (OA) (250 μl/kg) suspension in bovine serum albumin (BSA), (iii) dual hit: IT LPS (2 mg/kg) dissolved in NS and iv OA (100 μl/kg) and (iv) control group: IT NS and iv BSA. From each group at set periods of time various investigations like chest X-rays, respiratory rate (RR), tidal volume (TV), total cell count, differential cell count, total protein count and cytokine levels in bronchoalveolar lavage fluid (BALF), lung wet/dry weight ratio and histopathological examination were done. Results: It was noted that the respiratory rate, and tumour necrosis factor-α (TNF-α) levels were significantly higher at 4 h in the dual hit group as compared to LPS, OA and control groups. Interleukin-6 (IL-6) levels were significantly higher in the dual hit group as compared to LPS at 8 and 24 h, OA at 8 h and control (at all time intervals) group. IL-1β levels were significantly higher in LPS and dual hit groups at all time intervals, but not in OA and control groups. The injury induced in dual hit group was earlier and more sustained as compared to LPS and OA alone. Interpretation & conclusions: The lung pathology and changes in respiration functions produced by the dual hit model were closer to the diagnostic criteria of ALI/ARDS in terms of clinical manifestations and pulmonary injury and the injury persisted longer as compared to LPS and OA single hit model. Therefore, the ARDS model produced by the dual hit method was closer to the diagnostic criteria of ARDS in terms of clinical manifestations and pulmonary injury. © 2016, Indian Council of Medical Research. All rights reserved.

Gatne M.M.,Mumbai Veterinary College | Yadav M.H.,Mumbai Veterinary College | Mahale T.R.,Mumbai Veterinary College
Buffalo Bulletin | Year: 2012

The pharmacokinetics of flunixin after single intramuscular injection 2.2 mg/kg in six male buffalo calves was studied. Plasma flunixin concentration was analysed using a sensitive LC-MS/MS method with emtricitabine as internal standard. The limit of quantification for the method was 0.1 μg/ml. A set of eight calibrants ranging from 0.100 μg/ml to 19.996 μg/ml was used to plot a standard linear curve for quantifying flunixin with r2 value 0.9964. Pharmacokinetic parameters were determined for each buffalo calf using non-compartmental analysis and were calculated using WinNonlin.software Analysis of plasma showed rapid absorption, extensive distribution and slow elimination of flunixin following intramuscular administration in buffalo calves. Mean peak plasma flunixin concentration of 7.00±1.82 μg/ml occurred at a mean time of 0.67±0.11 h.

Badhe S.R.,Mumbai Veterinary College | Fairoze M.N.,Mumbai Veterinary College
Indian Journal of Animal Research | Year: 2012

The extracts of many spices and herbs have become popular in recent years for their antimicrobial and antioxidant properties. Cloves (Eugeniacaryophylata Thunb) of family Myrtaceae is a commonly used spice in India. A study on antimicrobial efficiency of clove powder on food borne pathogens viz., standard culture of Staphylococcus aureus, Salmonella typhimurium, Escherichia coli, and Bacillus cereus was carried out in this research work at refrigerated temperature (8±2°C) of interval 0, 3, 6, 12, 24, 48 hrs.whole chicken legs were marinating with 1 and 2 per cent clove powder. The present study showed that use of 2% level of clove powder was more effective against S. aureus followed by E.coli and Salmonella typhimurium under refrigeration (8±2°C) and significant reduction of bacterial counts was observed at 24 hr under refrigeration (8±2°C).

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