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Karabasanavar N.S.,Mumbai Veterinary College | Singh S.P.,University of Veterinary and Animal Sciences | Umapathi V.,Indian Council of Agricultural Research | Kumar D.,Indian Veterinary Research Institute | Shebannavar S.N.,Gennova Biopharmaceutical Pvt. Ltd.
Journal of Food Quality | Year: 2011

A highly species-specific polymerase chain reaction (PCR) assay was developed for the authentic identification of goat. A product of 436bp was amplified using newly designed primers against mitochondrial D-loop region. The possibility of cross-amplification was ruled out by considering as many as 25 other animal species. Suitability of the developed goat species-specific PCR assay was confirmed for in raw, cooked (60, 80 and 100C for 30min) and micro-oven-processed meat samples (n=20 each). A sensitivity of 0.1% was established for detection of adulteration and limit of detection of goat DNA was 0.1pg. This investigation presents a novel PCR assay with its newly designed primers that could be used for the authentic identification of goat species. © 2011 Wiley Periodicals, Inc..


Badhe S.R.,Mumbai Veterinary College | Fairoze M.N.,Mumbai Veterinary College
Indian Journal of Animal Research | Year: 2012

The extracts of many spices and herbs have become popular in recent years for their antimicrobial and antioxidant properties. Cloves (Eugeniacaryophylata Thunb) of family Myrtaceae is a commonly used spice in India. A study on antimicrobial efficiency of clove powder on food borne pathogens viz., standard culture of Staphylococcus aureus, Salmonella typhimurium, Escherichia coli, and Bacillus cereus was carried out in this research work at refrigerated temperature (8±2°C) of interval 0, 3, 6, 12, 24, 48 hrs.whole chicken legs were marinating with 1 and 2 per cent clove powder. The present study showed that use of 2% level of clove powder was more effective against S. aureus followed by E.coli and Salmonella typhimurium under refrigeration (8±2°C) and significant reduction of bacterial counts was observed at 24 hr under refrigeration (8±2°C).


Gatne M.M.,Mumbai Veterinary College | Yadav M.H.,Mumbai Veterinary College | Mahale T.R.,Mumbai Veterinary College
Buffalo Bulletin | Year: 2012

The pharmacokinetics of flunixin after single intramuscular injection 2.2 mg/kg in six male buffalo calves was studied. Plasma flunixin concentration was analysed using a sensitive LC-MS/MS method with emtricitabine as internal standard. The limit of quantification for the method was 0.1 μg/ml. A set of eight calibrants ranging from 0.100 μg/ml to 19.996 μg/ml was used to plot a standard linear curve for quantifying flunixin with r2 value 0.9964. Pharmacokinetic parameters were determined for each buffalo calf using non-compartmental analysis and were calculated using WinNonlin.software Analysis of plasma showed rapid absorption, extensive distribution and slow elimination of flunixin following intramuscular administration in buffalo calves. Mean peak plasma flunixin concentration of 7.00±1.82 μg/ml occurred at a mean time of 0.67±0.11 h.

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