Mourya D.T.,National Institute of Virology |
Yadav P.D.,National Institute of Virology |
Shete A.M.,National Institute of Virology |
Sathe P.S.,National Institute of Virology |
And 9 more authors.
Emerging Infectious Diseases | Year: 2015
We conducted a cross-sectional serosurvey of Crimean- Congo hemorrhagic fever (CCHF) among livestock in 22 states and 1 union territory of India. A total of 5,636 samples from bovines, sheep, and goats were screened for CCHF virus IgG. IgG was detected in 354 samples, indicating that this virus is widespread in this country. © 2015, Centers for Disease Control and Prevention (CDC). All rights reserved.
Sharma G.K.,Mukteswar |
Mahajan S.,Mukteswar |
Das B.,Mukteswar |
Ranjan R.,Mukteswar |
And 2 more authors.
OIE Revue Scientifique et Technique | Year: 2014
Multiplex reverse-transcription polymerase chain reaction (mRT-PCR) assay is a sensitive and rapid method for the detection and serotyping of foot and mouth disease virus (FMDV). However, the method has not been used to its full potential, because of factors such as cost, a lack of infrastructure and the complexity of the reaction mixture. This study was undertaken to optimise and validate a thermostable, lyophilised, ready-to-use mRT-PCR kit for the rapid detection of FMDV in field laboratories in India. Trehalose, PEG-8000 and glycerol were evaluated for stabilisation of the PCR mixture at ambient temperatures. The lyophilised mRT-PCR kit was validated and found robust enough for use in field-level laboratories. The PCR reaction mixture in the ready-to-use kit has low complexity, so chances of cross-contamination during the preparation of the mixture are limited, but may easily be monitored by using lyophilised internal positive and negative controls. In addition, the requirement to maintain live FMDV isolates as internal positive controls at field-level regional laboratories is eliminated.