Muhammadiyah University of Purwokerto
Purwokerto, Indonesia
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Diniatik K.,Gadjah Mada University | Diniatik K.,Muhammadiyah University of Purwokerto | Pramono S.,Gadjah Mada University | Riyanto S.,Gadjah Mada University
Asian Journal of Pharmaceutical and Clinical Research | Year: 2017

Objective: Stelechocarpus burahol, (Bl.) Hook f. & Th. is a plant widely distributed in Java Island of Indonesia. The aim of this study is to identify compounds from the leaves of S. burahol that exhibited activity as xanthine oxidase inhibitor (XOI). Methods: The leaves were extracted with aqueous ethanol and hydrolyzed with HCl methanol, then, partitioned sequentially with chloroform and ethyl acetate. The ethyl acetate fractions were separated by column chromatography with cellulose as stationary phase and methanol 50% as mobile phase. Results: Purification from this extracts afforded three compounds with one compound identified, namely kaempferol. The four compounds possessed as XOI with IC50 values ranging from 0.27 to 0.45 µg/ml. Conclusion: Kaempferol exhibited the highest inhibition of 0.27 µg/ml. © 2017 The Authors.

Mulia D.S.,Muhammadiyah University of Purwokerto | Latifah K.A.,Muhammadiyah University of Purwokerto | Purbomartono C.,Muhammadiyah University of Purwokerto | Maryanto H.,Muhammadiyah University of Purwokerto
AIP Conference Proceedings | Year: 2016

African catfish farming often find obstacle by disease problem, chiefly caused by Aeromonas hydrophila. The disease is known as Motile Aeromonas Septicemia (MAS) disease. The problem which often appear is the height of feed price, whereas the production price tends stable, besides the price of the feed reached 60-70% from the whole production price. As a result, fish farmer is often loss. It needs strategy to make feed from cheap material, but it still has good nutrient quality, even by using the waste, all at once giving immune impact for fish body that is by vaccinated feed-making. The feed made of chicken feathers, tahu dregs and trash fish mixed, then, added A. hydrophila polyvalent vaccine, so it produces vaccinated feed. The study aims to test the use of vaccinated feed to African catfish in Kebumen district. The test field was conducted in Jatijajar village, Ayah sub district, Kebumen district. The research used Completed Random Design (CRD) with three treatments and five times test. The treatment consists of P1: the distribution of vaccinated feed for 10 days; P2: the distribution of vaccinated feed for 15 days; and P3: control (non vaccine). The feed was given as many as 5%/BB/a fish/day. The research used plastic tarpaulin pool with the length x width x height: 60×60×80 cm. The African catfish used is fish in the age of two months, the length 12-16 cm with the weight 7,3-21,1 grams. The fish was protected for eight weeks. Parameter observed was immune response formed of antibody titers, the additional of weight and length, as well as fish survival. The supporting parameter observed was the parameter of water quality including water temperature, pH and dissolved oxygen. The analysis data used Analysis of Variance (Anova) and Duncan Multiple Range Test (DMRT) on test standard 5%. The result of the study showed that vaccinated feed could increase antibody titers production (P<0.05) compared the control. The test P1 (10 days vaccinated feed distribution) was more effective and efficient than with P2 (15 days vaccinated feed distribution). The use of vaccinated feed can be applied in the field. © 2016 Author(s).

Noviyana A.,Muhammadiyah University of Purwokerto | Noviyana A.,Brawijaya University | Nooryanto M.,Brawijaya University | Andarini S.,Brawijaya University
Clinical Nutrition Experimental | Year: 2017

Objective: The purpose of the present study was to analyze the effects of ferrous sulfate in different trimesters of pregnancy on oxidative stress and p-NF-κB p65 in the placenta. Material and methods: Twenty-four female Wistar rats were divided into four groups: the control group (no treatment), the group treated with ferrous sulfate from the first trimester (the first day of pregnancy), the second trimester (the eighth day of pregnancy) and the third trimester (the fifteenth day of pregnancy). Malondialdehyde levels as a marker of oxidative stress in the placenta was analyzed by a spectrophotometer. Placental expression of p-NF-κB p65 was analyzed by western blotting. Results: Placental MDA levels were significantly higher for the three groups of ferrous sulfate administration relative to the group without ferrous sulfate administration (p < 0.05). Placental MDA levels increased significantly for the groups treated with ferrous sulfate in trimesters one and two relative to that in trimester tree (p < 0.05). There were no significant differences in placental MDA levels between the groups treated with ferrous sulfate during the first and the second trimester (p > 0.05). Expression of p-NF-κB p65 decreased significantly for the groups treated with ferrous sulfate relative to that of controls (p < 0.05). There was a significant decreased in placental expression of p-NF-κB p65 for the group treated with ferrous sulfate in the first trimester relative to that in the second and third trimesters (p < 0.05). Expression of p-NF-κB p65 was significantly higher for the group treated with ferrous sulfate in the second trimester than that of the first trimester (p < 0.05). Conclusion: In conclusion, administration of ferrous sulfate will induce placental oxidative damage. Increased activation of NF-κB is underlain by changes in the degree of oxidative stress in the placenta from administration of ferrous sulfate. © 2017 The Authors.

Djalil A.D.,Bandung Institute of Technology | Djalil A.D.,Muhammadiyah University of Purwokerto | Nurulita N.A.,Muhammadiyah University of Purwokerto | Limantara L.W.,Ma Chung University | And 2 more authors.
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2012

Protoporphyrin IX (1), pheophorbide a (3), and its 1-hydroxyethyl derivativess (2,4) were studied in vitro as photosensitizer candidates for photodynamic therapy. Protoporphyrin IX has been indicated to have a cytotoxic effect in the absence of light excitation. The dark toxicity of 14 was evaluated against normal cells (Vero), human epithelial cervix carcinomas (HeLa) and human breast cancer (T47D) cell lines, while the phototoxicity of 1-4 was evaluated against HeLa and T47D cell lines. Moreover, the MTT assay was employed to evaluate cell viability. The 1-hydroxyethyl derivativess showed a lower dark toxicity in the three types of cells compared to the parent molecules. It was also observed that the parent molecules were more phototoxic than those of its 1-hydroxyethyl derivativess.

Ma'Ruf A.,Muhammadiyah University of Purwokerto | Ma'Ruf A.,Diponegoro University | Pramudono B.,Diponegoro University | Aryanti N.,Diponegoro University
AIP Conference Proceedings | Year: 2017

Biomass is one of abundance resources in the world. Biomass consists of three main materials such as cellulose, hemicelluloses and lignin. Therefore, biomass can be referred to lignocellulosic material. Both the cellulose and hemicelluloses fractions are polymers of sugars, and thereby a potential source of fermentable sugars, or other processes that convert sugars into products. Lignin is a polymer compound which contains of phenolic compounds. Rice husk is one of biomass, which has high contain of lignin. Rice husk has special characteristics because of silica content. The aim of this paper is to analyze lignin and silica extracted during lignin isolation process of rice husk using alkaline hydrogen peroxide. Three main variables such as solvent/solid ratio, concentration of hydrogen peroxide and pH of the mixture are studied. The optimum conditions for lignin isolation are at solvent/solid ratio 9:1 ml/gr, hydrogen peroxide concentration of 1.5%v and pH of the mixture of 11. © 2017 Author(s).

Anggoro S.,Indonesia University of Education | Anggoro S.,Muhammadiyah University of Purwokerto | Sopandi W.,Indonesia University of Education | Sholehuddin M.,Indonesia University of Education
Journal of Physics: Conference Series | Year: 2017

This study investigated the effects of joyful learning approach on elementary school students' attitudes toward science. The method used is quasy experiment with the participants were divided into two groups. Thirty three of 4th grade students volunteered as an experimental group, and the other forty two act as a control group. The data was collected by questionnaire that are given before and after the lesson, observation sheet, and interview. The effect of joyful learning on students' attitude was obtained by determining the n-gain and independent t-test. Observation and interview results were used to triangulate and support the quantitative findings. The data showed that the gain scores of the experimental group students' attitudes toward science were significantly higher than the gain scores of control group. In addition, the experimental group made significantly greater progress in their cognitive, affective and conative experiences. Interviews and observations indicated that their attitude toward science changed over the intervention. This indicated that joyful learning approach can enhance the elementary school students' attitudes toward science. According to these findings, it can be concluded that joyful learning approach can be used as an alternative approach to improve student's attitude toward science. © Published under licence by IOP Publishing Ltd.

PubMed | Padjadjaran University, University of Groningen and Muhammadiyah University of Purwokerto
Type: | Journal: Value in health regional issues | Year: 2016

Although cervical cancer is a preventable disease, the clinical and economic burdens of cervical cancer are still substantial issues in Indonesia.The main purpose of this study was to model the costs, clinical benefits, and cost-utility of both visual inspection with acetic acid (VIA) screening alone and human papillomavirus (HPV) vaccination in addition to VIA screening in Indonesia.We developed a population-based Markov model, consisting of three health states (susceptible, cervical cancer, and death), to assess future costs, health effects, and the cost-utility of cervical cancer prevention strategies in Indonesia. We followed a cohort of 100,000 females 12 to 100 years old and compared VIA screening alone with the addition of HPV vaccination on top of the screening to no intervention.The implementation of VIA screening alone and in combination with HPV vaccination would reduce the cervical cancer incidence by 7.9% and 58.5%, corresponding to 25 and 98 deaths avoided within the cohort of 100,000, respectively. We also estimated that HPV vaccination combined with VIA screening apparently yielded a lower incremental cost-effectiveness ratio at international dollar 1863/quality-adjusted life-year (QALY), compared with VIA screening alone (I$3126/QALY). Both strategies could however be definitely labeled as very cost-effective interventions, based on a threshold suggested by the World Health Organization. The incremental cost-effectiveness ratio was sensitive to the discount rate, cervical cancer treatment costs, and quality of life as part of the QALY.The addition of HPV vaccination on top of VIA screening could be a cost-effective strategy in Indonesia even if relatively conservative assumptions are applied. This population-based model can be considered as an essential tool to inform decision makers on designing optimal strategies for cervical cancer prevention in Indonesia.

Sisunandar,University of Queensland | Sisunandar,Muhammadiyah University of Purwokerto | Rival A.,CIRAD - Agricultural Research for Development | Turquay P.,CIRAD - Agricultural Research for Development | And 3 more authors.
Planta | Year: 2010

The present study aimed at exploring the fidelity of coconut (Cocos nucifera L.) plants recovered from cryopreservation. Zygotic embryos from various different cultivars were cryopreserved following four successive steps, namely: rapid dehydration, rapid freezing, rapid thawing and in vitro recovery followed by acclimatization. At the end of the acclimatization period, the seedlings were compared to counterparts of the same age, which were produced from non-cryopreserved embryos. Both series were submitted to morphological, cytological and molecular comparisons. No significant differences in terms of growth rates could be measured. In addition, no morphological variation could be detected through the measurement of shoot elongation rates, production of opened leaves, and the number and total length of primary roots. Karyotype analysis revealed the same chromosome number (2n = 32) in all studied cultivars independently of cryopreservation. No significant differences could be observed between control and cryopreserved material concerning the type of chromosomes, the length of the long and short arms, the arm length ratio and the centromeric index. However, idiogram analysis did show a greater number of black banding on chromosomes isolated from cryopreserved material. Genetic and epigenetic fidelity was assessed through microsatellite (SSR) analysis and global DNA methylation rates; no significant differences would be observed between genomic DNAs isolated from seedlings originating from cryopreserved embryos and respective controls. In conclusion, our results suggest that the method of cryopreservation under study did not induce gross morphological, genetic or epigenetic changes, thus suggesting that it is an appropriate method to efficiently preserve coconut germplasm. © 2010 Springer-Verlag.

Astuti H.J.,Muhammadiyah University of Purwokerto | Nagase K.,Kanazawa University
Health Services Management Research | Year: 2016

This paper develops a framework with which to conceptualize loyalty to healthcare organizations in terms of relationship marketing, patient satisfaction, provider switching, and retention programs. This conceptual framework can be viewed from the perspective of hospitals and clinics using structural equation modeling with a partial least squares approach (Smart PLS 2.0). Respondents received healthcare services, funded by the government and a university health insurance plan, from both types of organizations. The concept of loyalty differed slightly depended on whether data from the hospital and the clinic were analyzed together or separately. Indeed, hospitals and clinics differ with regard to several factors that can affect loyalty. © The Author(s) 2016.

Putri H.,Muhammadiyah University of Purwokerto | Cahyani P.C.,Muhammadiyah University of Purwokerto
International Journal on Advanced Science, Engineering and Information Technology | Year: 2016

Agricultural product is commodity which tends to fluctuate. Price volatility is caused by the change of agricultural production due to climate change as well as pest and disease. Furthermore, it is also caused by the change of agricultural land and high demand of agricultural products on religious holidays. This study was conducted to examine how volatile some of main food commodities in Banyumas Regency. Secondary data analysis method with quantitative approach was used in this research. Time series data of some food commodity prices (rice IR 64, local soybean, maize, chili red peppers, onion and garlic) from January 2008 - December 2013 were utilized. The coefficient of variation was calculated to determine price volatility. The result showed that the price of red chili pepper, onion and garlic was tending to volatile. The coefficient of variation ratio of those commodities was about 20% - 35%. While the price of rice, local soybean and maize was stable. The coefficient of variation ratio of those commodities was less than 9%. This study also includes some policies that can be suggested to prevent price volatility.

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