Muhammadiyah University of Purwokerto
Purwokerto, Indonesia
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Setiawan D.,University of Groningen | Setiawan D.,Muhammadiyah University of Purwokerto | Kotsopoulos N.,University of Groningen | Wilschut J.C.,University of Groningen | And 2 more authors.
PLoS ONE | Year: 2016

Background Cervical cancer poses a substantial burden in terms of morbidity, mortality, and economic losses, especially in low/middle-income countries. HPV vaccination and/or cervical cancer screening among females may reduce the burden of HPV-related diseases, including cervical cancer. However, limited funds may impede the implementation of population-based programmes. Governmental investments in the prevention of infectious disease may have broader economic and fiscal benefits, which are not accounted in conventional economic analyses. This study estimates the broader economic and fiscal impacts of implementing HPV vaccination and/or cervical cancer screening in Indonesia from the perspective of the government. Methods A government-perspective quantitative analytic framework was applied to assess the Net Present Value (NPV) of investment on cervical cancer prevention strategies including HPV vaccination, cervical screening and its combination in Indonesia. All monetary values were presented in International Dollars (I$). Results Based on a cohort of 10,000,000 Indonesian 12-year-old females, it was estimated that HPV vaccination and/or cervical cancer screening result in a positive NPV for the Indonesian government. The combination of cervical screening and HPV vaccination generated a substantial reduction of cervical cancer incidence and HPV-related mortality of 87,862 and 19,359, respectively. It was estimated that HPV vaccination in combination with cervical screening is the most favorable option for cervical cancer prevention (NPV I $2.031.786.000), followed by HPV vaccination alone (NPV I$1.860.783.000) and cervical screening alone (NPV I$375.244.000).Conclusion In addition to clinical benefits, investing in HPV vaccination and cervical screening may yield considerable fiscal benefits for the Indonesian governments due to lifelong benefits resulting from reduction of cervical cancer-related morbidity and mortality. © 2016 Setiawan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PubMed | Padjadjaran University, University of Groningen and Muhammadiyah University of Purwokerto
Type: | Journal: Value in health regional issues | Year: 2016

Although cervical cancer is a preventable disease, the clinical and economic burdens of cervical cancer are still substantial issues in Indonesia.The main purpose of this study was to model the costs, clinical benefits, and cost-utility of both visual inspection with acetic acid (VIA) screening alone and human papillomavirus (HPV) vaccination in addition to VIA screening in Indonesia.We developed a population-based Markov model, consisting of three health states (susceptible, cervical cancer, and death), to assess future costs, health effects, and the cost-utility of cervical cancer prevention strategies in Indonesia. We followed a cohort of 100,000 females 12 to 100 years old and compared VIA screening alone with the addition of HPV vaccination on top of the screening to no intervention.The implementation of VIA screening alone and in combination with HPV vaccination would reduce the cervical cancer incidence by 7.9% and 58.5%, corresponding to 25 and 98 deaths avoided within the cohort of 100,000, respectively. We also estimated that HPV vaccination combined with VIA screening apparently yielded a lower incremental cost-effectiveness ratio at international dollar 1863/quality-adjusted life-year (QALY), compared with VIA screening alone (I$3126/QALY). Both strategies could however be definitely labeled as very cost-effective interventions, based on a threshold suggested by the World Health Organization. The incremental cost-effectiveness ratio was sensitive to the discount rate, cervical cancer treatment costs, and quality of life as part of the QALY.The addition of HPV vaccination on top of VIA screening could be a cost-effective strategy in Indonesia even if relatively conservative assumptions are applied. This population-based model can be considered as an essential tool to inform decision makers on designing optimal strategies for cervical cancer prevention in Indonesia.

Nurulita N.A.,Gadjah Mada University | Nurulita N.A.,Muhammadiyah University of Purwokerto | Meiyanto E.,Gadjah Mada University | Sugiyanto,Gadjah Mada University | And 2 more authors.
Oriental Pharmacy and Experimental Medicine | Year: 2012

Recent studies both in vitro and in vivo of G. procumbens exhibits chemopreventive properties for tumor inhibition on several types of cancer. Our study was carried out to observe the anticancer property of ethyl acetate fraction of G. procumbens leaves (FEG) on breast cancer cells as well as the co-chemotherapeutic potential, and to investigate its molecular mechanisms. 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to measure the growth inhibitory effect of FEG, doxorubicin (DOX), and 5-fluorouracil (5-FU) and their combination. Flowcytometry, 4′,6-diamidino-2-phenylindole (DAPI) staining, and immunobloting were used to explore the mechanism of cell cycle arrest and apoptosis. FEG inhibited cell proliferation, induced G 1 phase arrest and apoptosis. The inhibitory effect of FEG was enhanced when combined with Dox and 5-FU. The apoptosis induction was related to the increase of c-PARP expression after combination treatment of FEG and Dox or 5-FU on MCF-7 cells. However, treatment of DOX, 5-FU, and FEG on T47D cells, resulting no significance DNA fragmentation and nuclei condensation evidance. Only combination treatment of 5-FU + FEG showed c-PARP expression in T47D cells. In T47D cells, The FEG treatment also caused the decrease of microtubule expression as shown by Western blotting assay. The decreasing level of microtubul expression might be caused by protein aggregation, as shown by immunostaning using α-tubulin antibody. All these results suggest that FEG potentiates the DOX and 5-FU efficacy on MCF-7 and T47D cells. FEG induces T47D cell death through different mechanism than MCF-7 that proposed to be mitotic catastrophe. The FEG may have specific targeted on microtubule integrity modulation leading to the cell cycle arrest and proliferation inhibition. Further FEG could be developed as a co-chemotherapeutic agent for reducing side effect and have specific molecular target for breast cancer. © 2012 Institute of Oriental Medicine, Kyung Hee University.

Febriansah R.,Gadjah Mada University | Febriansah R.,Muhammadiyah University of Yogyakarta | Putri D.D.P.,Gadjah Mada University | Sarmoko,Gadjah Mada University | And 5 more authors.
Asian Pacific Journal of Tropical Biomedicine | Year: 2014

Objective: To evaluate of hesperidin to overcome resistance of doxorubicin in MCF-7 resistant doxorubicin cells (MCF-7/Dox) in cytotoxicity apoptosis and P-glycoprotein (Pgp) expression in combination with doxorubicin. Methods: The cytotoxic properties, 50% inhibition concentration (IC50) and its combination with doxorubicin in MCF-7 cell lines resistant to doxorubicin (MCF-7/Dox) cells were determined using MTT assay. Apoptosis induction was examined by double staining assay using ethidium bromide-acridine orange. Immunocytochemistry assay was performed to determine the level and localization of Pgp. Results: Single treatment of hesperidin showed cytotoxic activity on MCF-7/Dox cells with IC50 value of 11 μmol/L. Thus, combination treatment from hesperidin and doxorubicin showed addictive and antagonist effect (CI > 1.0). Hesperidin did not increase the apoptotic induction, but decreased the Pgp expressions level when combined with doxorubicin in low concentration. Conclusions: Hesperidin has cytotoxic effect on MCF-7/Dox cells with IC50 of 11 μmol/L. Hesperidin did not increased the apoptotic induction combined with doxorubicin. Co-chemotherapy application of doxorubicin and hesperidin on MCF-7/Dox cells showed synergism effect through inhibition of Pgp expression. © 2014 by the Asian Pacific Journal of Tropical Biomedicine.

Sisunandar,University of Queensland | Sisunandar,Muhammadiyah University of Purwokerto | Rival A.,CIRAD - Agricultural Research for Development | Turquay P.,CIRAD - Agricultural Research for Development | And 3 more authors.
Planta | Year: 2010

The present study aimed at exploring the fidelity of coconut (Cocos nucifera L.) plants recovered from cryopreservation. Zygotic embryos from various different cultivars were cryopreserved following four successive steps, namely: rapid dehydration, rapid freezing, rapid thawing and in vitro recovery followed by acclimatization. At the end of the acclimatization period, the seedlings were compared to counterparts of the same age, which were produced from non-cryopreserved embryos. Both series were submitted to morphological, cytological and molecular comparisons. No significant differences in terms of growth rates could be measured. In addition, no morphological variation could be detected through the measurement of shoot elongation rates, production of opened leaves, and the number and total length of primary roots. Karyotype analysis revealed the same chromosome number (2n = 32) in all studied cultivars independently of cryopreservation. No significant differences could be observed between control and cryopreserved material concerning the type of chromosomes, the length of the long and short arms, the arm length ratio and the centromeric index. However, idiogram analysis did show a greater number of black banding on chromosomes isolated from cryopreserved material. Genetic and epigenetic fidelity was assessed through microsatellite (SSR) analysis and global DNA methylation rates; no significant differences would be observed between genomic DNAs isolated from seedlings originating from cryopreserved embryos and respective controls. In conclusion, our results suggest that the method of cryopreservation under study did not induce gross morphological, genetic or epigenetic changes, thus suggesting that it is an appropriate method to efficiently preserve coconut germplasm. © 2010 Springer-Verlag.

Haryanto,Muhammadiyah University of Purwokerto | Haryanto,Yeungnam University | Singh D.,Yeungnam University | Huh P.H.,Pusan National University | Kim S.C.,Yeungnam University
Journal of biomedical materials research. Part A | Year: 2016

A microporous hydrogel scaffold was developed from hyperbranched poly(glycidol) (HPG) and poly(ethylene oxide) (PEO) using electron beam (e-beam) induced cross-linking for tissue engineering applications. In this study, HPG was synthesized from glycidol using trimethylol propane as a core initiator and cross-linked hydrogels were made using 0, 10, 20, and 30% HPG with respect to PEO. The effects of %-HPG on the swelling ratio, cross-linking density, mechanical properties, morphology, degradation, and cytotoxicity of the hydrogel scaffolds were then investigated. Increasing the HPG content increased the pore size of the hydrogel scaffold, as well as the porosity, elongation at break, degree of degradation and swelling ratio. In contrast, the presence of HPG decreased the cross-linking density of the hydrogel. There was no significant difference in compressive modulus and tensile strength of all compositions. The pore size of hydrogel scaffolds could be easily tailored by controlling the content of HPG in the polymer blend. Evaluation of the cytotoxicity demonstrated that HPG/PEO hydrogel scaffold has potential for use as a matrix for cellular attachment and proliferation. These results indicate that cross-linked HPG/PEO hydrogel can function as a potential material for tissue engineering scaffolds. Moreover, a facile method to prepare hydrogel microporous scaffolds for tissue engineering by e-beam irradiation was developed. © 2015 Wiley Periodicals, Inc.

Astuti H.J.,Muhammadiyah University of Purwokerto | Nagase K.,Kanazawa University
Health Services Management Research | Year: 2016

This paper develops a framework with which to conceptualize loyalty to healthcare organizations in terms of relationship marketing, patient satisfaction, provider switching, and retention programs. This conceptual framework can be viewed from the perspective of hospitals and clinics using structural equation modeling with a partial least squares approach (Smart PLS 2.0). Respondents received healthcare services, funded by the government and a university health insurance plan, from both types of organizations. The concept of loyalty differed slightly depended on whether data from the hospital and the clinic were analyzed together or separately. Indeed, hospitals and clinics differ with regard to several factors that can affect loyalty. © The Author(s) 2016.

Hamad A.,Muhammadiyah University of Purwokerto | Alifah A.,Muhammadiyah University of Purwokerto | Permadi A.,Muhammadiyah University of Purwokerto | Hartanti D.,Muhammadiyah University of Purwokerto
International Food Research Journal | Year: 2016

Crude extract and essential oils from dried rhizome of two Zingiberaceae family plants, Alpinia galanga and Zingiber officinale, were evaluated for their chemical constituents and antimicrobial activity. The essential oils were analyzed by Gas Chromatography - Mass Spectroscopy (GCMS). The abundant constituents for A. galanga were cineole, 4-allylphenyl acetate, α-farnesene, (2,6-dimethylphenyl)borate and α-pinene; and for Z. officinale were cineole, 2,2-dimethyl-3-methylenenorbornane, α-curcumenene, β-sesquiphellandrene and rosefuran epoxide. Alpha-pinene, 2,2-dimethyl-3-methylenenorbornane, β-pinene, β-mircene, cineole, β-citral, α-citral, bornyl acetate, α-curcumene, α-zingiberene, β-sesquiphellandrene, and hexadecanoic acid are detected in both galangal and ginger rhizomes. Minimum Inhibitory Concentration (MIC) of essential oils and crude extracts were evaluated by broth dillution method against foodborne bacteria Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Salmonella typhymurium and Vibrio cholera. MIC of crude extract and essential oils of galangal and ginger against all tested microogranisms were relatively high. © 2008 IFRJ.

Putri H.,Muhammadiyah University of Purwokerto | Cahyani P.C.,Muhammadiyah University of Purwokerto
International Journal on Advanced Science, Engineering and Information Technology | Year: 2016

Agricultural product is commodity which tends to fluctuate. Price volatility is caused by the change of agricultural production due to climate change as well as pest and disease. Furthermore, it is also caused by the change of agricultural land and high demand of agricultural products on religious holidays. This study was conducted to examine how volatile some of main food commodities in Banyumas Regency. Secondary data analysis method with quantitative approach was used in this research. Time series data of some food commodity prices (rice IR 64, local soybean, maize, chili red peppers, onion and garlic) from January 2008 - December 2013 were utilized. The coefficient of variation was calculated to determine price volatility. The result showed that the price of red chili pepper, onion and garlic was tending to volatile. The coefficient of variation ratio of those commodities was about 20% - 35%. While the price of rice, local soybean and maize was stable. The coefficient of variation ratio of those commodities was less than 9%. This study also includes some policies that can be suggested to prevent price volatility.

Mustafidah H.,Muhammadiyah University of Purwokerto | Suwarsito,Muhammadiyah University of Purwokerto
2010 International Conference on Distributed Frameworks for Multimedia Applications, DFmA 2010 | Year: 2010

At the present time, fish disease information in Indonesia is limited and just documented partially in research reports, journals, and books. Otherwise, the latest and complete information about problem of fish disease were difficult to get by fish farmers, related agencies, and research groups. The limited dissemination of information on fish diseases had caused difficulties to controlling fish diseases in Indonesia. Therefore, it was needed to develop the computer programs or software to identify and controlling fish diseases in Indonesia. Software design method was using Based Reasoning Case (CBR). CBR scheme includes retrieve, reuse, revise, and retain. The retrieve stage of the cases used the nearest neighbor technique. This system has been built conceptually as basis for further programming development. By these methods, a variety of fish disease cases will be processed by computer systems based on similar cases that occurred formerly, and will be given the appropriate solution. © 2010 University Sains Malaysia.

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