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Sisunandar,University of Queensland | Sisunandar,Muhammadiyah University of Purwokerto | Sopade P.A.,University of Queensland | Samosir Y.M.S.,University of Queensland | And 3 more authors.
Cryo-Letters | Year: 2012

Protocols are proposed for the low (-20°C) and ultra-low (-80°C) temperature storage of coconut (Cocos nucifera L.) embryos. A tissue dehydration step prior to storage, and a rapid warming step upon recovery optimized the protocol. The thermal properties of water located within the embryos were monitored using differential scanning calorimetry (DSC). In the most efficient version of the protocol, embryos were dehydrated under a sterile air flow in a dehydration solution containing glucose (3.33 M) and glycerol (15%) for 16 hours. This protocol decreased the embryo water content from 77 to 29% FW and at the same time reduced the amount of freezable water down to 0.03%. The dehydrated embryos could be stored for up to 3 weeks at -20°C (12% producing normal plants upon recovery) or 26 weeks at -80°C (28% producing normal plants upon recovery). These results indicate that it is possible to store coconut germplasm on a medium term basis using an ultra-deep freezer unit. However for more efficient, long term storage, cryopreservation remains the preferred option. © CryoLetters. Source

Febriansah R.,Gadjah Mada University | Febriansah R.,Muhammadiyah University of Yogyakarta | Putri D.D.P.,Gadjah Mada University | Sarmoko,Gadjah Mada University | And 5 more authors.
Asian Pacific Journal of Tropical Biomedicine | Year: 2014

Objective: To evaluate of hesperidin to overcome resistance of doxorubicin in MCF-7 resistant doxorubicin cells (MCF-7/Dox) in cytotoxicity apoptosis and P-glycoprotein (Pgp) expression in combination with doxorubicin. Methods: The cytotoxic properties, 50% inhibition concentration (IC50) and its combination with doxorubicin in MCF-7 cell lines resistant to doxorubicin (MCF-7/Dox) cells were determined using MTT assay. Apoptosis induction was examined by double staining assay using ethidium bromide-acridine orange. Immunocytochemistry assay was performed to determine the level and localization of Pgp. Results: Single treatment of hesperidin showed cytotoxic activity on MCF-7/Dox cells with IC50 value of 11 μmol/L. Thus, combination treatment from hesperidin and doxorubicin showed addictive and antagonist effect (CI > 1.0). Hesperidin did not increase the apoptotic induction, but decreased the Pgp expressions level when combined with doxorubicin in low concentration. Conclusions: Hesperidin has cytotoxic effect on MCF-7/Dox cells with IC50 of 11 μmol/L. Hesperidin did not increased the apoptotic induction combined with doxorubicin. Co-chemotherapy application of doxorubicin and hesperidin on MCF-7/Dox cells showed synergism effect through inhibition of Pgp expression. © 2014 by the Asian Pacific Journal of Tropical Biomedicine. Source

Hardani D.N.K.,Gadjah Mada University | Hardani D.N.K.,Muhammadiyah University of Purwokerto | Wahyunggoro O.,Gadjah Mada University | Nugroho H.A.,Gadjah Mada University | Faisal N.,Gadjah Mada University
Proceedings of 2014 International Conference on Electrical Engineering and Computer Science, ICEECS 2014 | Year: 2014

Emotion is a mental condition that appears spontaneously based on self-conscious effort and usually followed by physiological changes. Emotion is especially caused by stimulation. The related information with emotional condition by someone is communicated to all body through ECG. Therefore, it is important to conduct a research in analysis of emotion based on the measurement of ECG. Data emotion was categorised according to the stimulation that had been given to the subject by using video and music as long as ECG recorded. ICA method analysis with FastICA algorithm could be developed to obtain emotion feature. Feature classification was based on statistical approach from the independent component with higher of kurtosis value. The proposed method in classification was based on decision tree using Random Forest algorithm. The classification result shows that the emotional recognition based on ECG signals can be well implemented by system. The developed method successfully classifies the emotional condition from ECG signals. The method achieves the accuracy of 92.2% for identification of neutral emotion, 93.9% for negative emotion and 92.1% for positive emotion. The value of ICSI is obtained about 81.2% for neutral conditions, 88.3% for negative emotions and 85.1% for positive emotions, it means that the system is successfully to classify individually and effective for overall. © 2014 IEEE. Source

Nurulita N.A.,Gadjah Mada University | Nurulita N.A.,Muhammadiyah University of Purwokerto | Meiyanto E.,Gadjah Mada University | Sugiyanto,Gadjah Mada University | And 2 more authors.
Oriental Pharmacy and Experimental Medicine | Year: 2012

Recent studies both in vitro and in vivo of G. procumbens exhibits chemopreventive properties for tumor inhibition on several types of cancer. Our study was carried out to observe the anticancer property of ethyl acetate fraction of G. procumbens leaves (FEG) on breast cancer cells as well as the co-chemotherapeutic potential, and to investigate its molecular mechanisms. 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to measure the growth inhibitory effect of FEG, doxorubicin (DOX), and 5-fluorouracil (5-FU) and their combination. Flowcytometry, 4′,6-diamidino-2-phenylindole (DAPI) staining, and immunobloting were used to explore the mechanism of cell cycle arrest and apoptosis. FEG inhibited cell proliferation, induced G 1 phase arrest and apoptosis. The inhibitory effect of FEG was enhanced when combined with Dox and 5-FU. The apoptosis induction was related to the increase of c-PARP expression after combination treatment of FEG and Dox or 5-FU on MCF-7 cells. However, treatment of DOX, 5-FU, and FEG on T47D cells, resulting no significance DNA fragmentation and nuclei condensation evidance. Only combination treatment of 5-FU + FEG showed c-PARP expression in T47D cells. In T47D cells, The FEG treatment also caused the decrease of microtubule expression as shown by Western blotting assay. The decreasing level of microtubul expression might be caused by protein aggregation, as shown by immunostaning using α-tubulin antibody. All these results suggest that FEG potentiates the DOX and 5-FU efficacy on MCF-7 and T47D cells. FEG induces T47D cell death through different mechanism than MCF-7 that proposed to be mitotic catastrophe. The FEG may have specific targeted on microtubule integrity modulation leading to the cell cycle arrest and proliferation inhibition. Further FEG could be developed as a co-chemotherapeutic agent for reducing side effect and have specific molecular target for breast cancer. © 2012 Institute of Oriental Medicine, Kyung Hee University. Source

Nguyen Q.T.,University of Queensland | Nguyen Q.T.,Ho Chi Minh City International University | Bandupriya H.D.D.,Coconut Research Institute | Lopez-Villalobos A.,University of Calgary | And 3 more authors.
Planta | Year: 2015

Main conclusion: The present review discusses not only advances in coconut tissue culture and associated biotechnological interventions but also future research directions toward the resilience of this important palm crop. Coconut (Cocos nucifera L.) is commonly known as the ‘tree of life’. Every component of the palm can be used to produce items of value and many can be converted into industrial products. Coconut cultivation faces a number of acute problems that reduce its productivity and competitiveness. These problems include various biotic and abiotic challenges as well as an unstable market for its traditional oil-based products. Around 10 million small-holder farmers cultivate coconut palms worldwide on c. 12 million hectares of land, and many more people own a few coconut palms that contribute to their livelihoods. Inefficiency in the production of seedlings for replanting remains an issue; however, tissue culture and other biotechnological interventions are expected to provide pragmatic solutions. Over the past 60 years, much research has been directed towards developing and improving protocols for (i) embryo culture; (ii) clonal propagation via somatic embryogenesis; (iii) homozygote production via anther culture; (iv) germplasm conservation via cryopreservation; and (v) genetic transformation. Recently other advances have revealed possible new ways to improve these protocols. Although effective embryo culture and cryopreservation are now possible, the limited frequency of conversion of somatic embryos to ex vitro seedlings still prevents the large-scale clonal propagation of coconut. This review illustrates how our knowledge of tissue culture and associated biotechnological interventions in coconut has so far developed. Further improvement of protocols and their application to a wider range of germplasm will continue to open up new horizons for the collection, conservation, breeding and productivity of coconut. © 2015, Springer-Verlag Berlin Heidelberg. Source

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