Meng X.-X.,Mudanjiang Teachers College |
Tian S.-G.,Mudanjiang Medical University
19th International Conference on Industrial Engineering and Engineering Management: Engineering Management | Year: 2013
In the reason of the deficiencies in their own areas, traditional costing methods cannot accurately measure the cost of logistics. It urgently asks us to find new methods to measure logistics cost accurately and effectively. The application of activity-based costing to logistics management is keeping with this request. The principles of activity-based costing are expounded firstly, then this text intends to identify that activity-based costing have also its applicability in the logistics cost management in comparison with traditional costing. Finally two methods are compared and result of testing is analyzed. This text is focused on the application of activity-based costing to calculate logistics cost, which solves the problem of how to combine activity-based cost with practice. © 2013 Springer-Verlag Berlin Heidelberg.
Wang X.,Liaoning University of Traditional Chinese Medicine |
Yu S.,Liaoning University of Traditional Chinese Medicine |
Gao S.-J.,Second Staff Hospital of Liaohe Oilfield Company |
Hu J.-P.,Mudanjiang Medical University |
And 2 more authors.
Neuroendocrinology Letters | Year: 2014
OBJECTIVE: Amyloid-beta (Aβ) is a 36-43 amino acid peptide that is derived by processing of the beta-amyloid precursor protein (APP). Aβ plays a central role in the development of Alzheimer's disease (AD). Although growing evidence suggests that insulin has important functions in Aβ metabolism, the underlying mechanisms are still unknown. METHODS: Using an SH-SY5Y cell line overexpressing human APP Swedish mutant (APPsw), we evaluated the effect of insulin on APP processing and Aβ production by using western blot analysist. RESULTS: Our data showed that administration of insulin reduced the Aβ generation in culture media with a concomitant decreases in the levels of β-secretase BACE1, secreted extracellular domain (sAPPβ) and a fragment of 99 amino acids (C99) in APPsw cells. We further showed that insulin increased the levels of α-secretase ADAM10, a secreted extracellular domain secreted (sAPPα) and a fragment of 83 amino acids (C83) in APPsw cells. CONCLUSION: Our present data suggest that insulin could inhibit Aβ production through modulation of APP processing by increasing cleavage at the α-secretase site and decreased cleavage at the β-secretase sites. © 2014 Neuroendocrinology Letters.
Feng B.,University of Western Ontario |
Cao Y.,University of Western Ontario |
Cao Y.,Mudanjiang Medical University |
Chen S.,University of Western Ontario |
And 2 more authors.
Life Sciences | Year: 2014
Aims MicroRNAs (miRNAs) play important roles in several biological processes. In this study, we investigated the role of miR-1, an endothelin-1 (ET-1) targeting miRNA, in endothelial cells (ECs) and tissues of diabetic animals. ET-1 is known to be of pathogenetic significance in several chronic diabetic complications. Main methods PCR array was used to identify alterations of miRNA expression in ECs exposed to glucose. miR-1 expression was validated by TaqMan real-time PCR assay. Human retinal ECs (HRECs) and human umbilical vein ECs (HUVECs) exposed to various glucose levels with or without miR-1 mimic transfection, and tissues from streptozotocin-induced diabetic animals after two months of follow-up, were examined for miR-1 expression, as well as ET-1 and fibronectin (FN) mRNA and protein levels. Key findings Array analyses showed glucose-induced alterations of 125 miRNAs (out of 381) in ECs exposed to 25 mM glucose compared to 5 mM glucose. Fifty-one miRNAs were upregulated and 74 were downregulated. 25 mM glucose decreased miR-1 expression and increased ET-1 mRNA and protein levels. miR-1 mimic transfection prevented HG-induced ET-1 upregulation. Furthermore, glucose induced upregulation of FN, which is mediated partly by ET-1, was also prevented by such transfection. Diabetic animals showed decreased miR-1 expression in the retina, heart and kidneys. In parallel, ET-1 mRNA expressions were increased in these tissues of diabetic animals, in association with upregulation of FN. Significance These results indicate a novel glucose-induced mechanism of tissue damage, in which miR-1 regulates ET-1 expressions in diabetes. Identifying such mechanisms may lead to RNA based treatment for diabetic complications. © 2014 Elsevier Inc. All rights reserved.
Zhang R.,Shenyang University |
Zhang R.,Mudanjiang Medical University |
Yang G.,Shenyang University |
Wang Q.,Mudanjiang Medical University |
And 2 more authors.
Molecular Biology Reports | Year: 2013
Ghrelin has two major molecular forms, acylated ghrelin (AG) and unacylated ghrelin (UAG). Only AG to bind growth hormone secretagogue receptor 1a (GHSR-1a) has central endocrine activities. An antiapoptotic effect of AG in cortical neuronal cells has recently been reported. However, whether there is a neuroprotective effect of AG in hippocampal neurons of pilocarpine-induced seizures in rats, is still unknown. Therefore, in the present study, the underlying mechanism of AG on lithium-pilocarpine-induced excitotoxicity was examined in the hippocampus of rat. The results showed that AG inhibited pilocarpine-induced apoptosis. Exposure of rats to the receptor-specific antagonist D-Lys-3-GHRH-6 abolished the protective effects of AG against epilepsy. Administration of AG resulted in increased expression of phosphor-Akt in status epilepticus model in rats, which was accompanied with the attenuation of hippocampal cell death. Furthermore, administration of AG resulted in decreased expression of phosphor-JNK in pyramidal neurons of hippocampus after status epilepsy, which was also accompanied with the attenuation of hippocampal cell death, too. In addition, AG increased the Bcl-2/Bax ratio and inhibited caspase-3 activation. The data indicate that AG can function as a neuroprotective agent that inhibits apoptotic pathways. These effects may be mediated via activation of the PI3K/Akt pathway. © 2012 Springer Science+Business Media Dordrecht.
Ma F.,Harbin Medical University |
Song H.,Harbin Medical University |
Guo B.,Harbin Medical University |
Zhang Y.,Harbin Medical University |
And 10 more authors.
Oncotarget | Year: 2015
MicroRNAs (miRs) function as key regulators of gene expression and their deregulation is associated with the carcinogenesis of various cancers. In the present study, we investigated the biological role and mechanism of miR-361-5p in colorectal carcinoma (CRC) and gastric cancer (GC). We showed that microRNA-361-5p (miR-361-5p) was down-regulated in CRC and GC in comparison to the controls. Meanwhile, the expression levels of miR-361-5p negatively correlated with lung metastasis and prognosis in clinical CRC patients. Overexpression of miR-361-5p markedly suppressed proliferation, migration and invasion of cancer cells. Additionally, this phenotype could be partially rescued by the ectopic expression of staphylococcal nuclease domain containing-1 (SND1). SND1 was identified as a target of miR-361-5p using bioinformatics analysis and in vitro luciferase reporter assays. In turn, SND1 bound to pre-miR-361-5p and suppressed the expression of miR-361-5p, thus exerting a feedback loop. Most interestingly, in vivo studies showed that restoration of miR-361-5p significantly inhibited tumor growth and especially the lung metastasis in nude mice. Therefore, it could be concluded that miR-361-5p functions as a tumor-suppressive miRNA through directly binding to SND1, highlighting its potential as a novel agent for the treatment of patients with CRC and GC.
An J.,Key Laboratory of Cancer Prevention and Treatment of Heilongjiang Province |
An J.,Mudanjiang Medical University |
Zhu X.,Key Laboratory of Cancer Prevention and Treatment of Heilongjiang Province |
Wang H.,Mudanjiang Medical University |
Jin X.,Key Laboratory of Cancer Prevention and Treatment of Heilongjiang Province
International Journal of Oncology | Year: 2013
Alternative polyadenylation and microRNA regulation are both mechanisms of post-transcriptional regulation of gene expression. Alternative polyadenylation often results in mRNA isoforms with the same coding sequence but different lengths of 3' UTRs, while microRNAs regulate gene expression by binding to specific mRNA 3' UTRs. In this sense, different isoforms of an mRNA may be differentially regulated by microRNAs, sometimes resulting in cellular proliferation and this mechanism is being speculated on as a potential cause for cancer development.
Li X.-D.,Harbin Medical University |
Li Z.-G.,Harbin Medical University |
Song X.-X.,Mudanjiang Medical University |
Liu C.-F.,Harbin Medical University
Pathology | Year: 2010
Aims: Cirrhosis is an important risk factor for hepatocellular carcinoma (HCC) in China, while little is known of the genetic susceptibility to hepatocarcinogenesis. Traditional approaches to identification of novel genetic predisposition genes have focused on protein encoding genes. There is evidence to suggest that microRNAs (miRNA) may play an important role in tumorigenesis. Recent studies have implicated that the rs11614913 SNP in miR-196a2 may be associated with susceptibility to lung cancer, congenital heart disease, breast cancer, as well as reduced survival in non-small cell lung cancer. This study aims to assess whether this functional polymorphism can influence susceptibility to and the progression of cirrhosis-related HCC. Methods: 532 patients with hepatic cirrhosis (310 patients with HCC and 222 patients without HCC) were enrolled. DNA was extracted from blood specimens, and miR-196a2 polymorphism was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). In addition, tumour tissues of liver (n59) were obtained from the studied HCC patients for measurement of miR-196a expression levels. Results: The frequency of the CC genotype among HCC patients was higher than that in the control group, implying that the cirrhotic patients with the CC genotype or C allele containing genotypes (CT and CC) may have a higher risk of HCC. However, in a subsequent analysis of the association between this polymorphism and clinicopathological characteristics, there was an association between rs11614913 genotype and tumour size (p=0.046), but not with tumour number, grade, stage, invasiveness or Child-Pugh grade. In a genotype-phenotype correlation analysis using 59 tumour tissues of liver, rs11614913 CC or carrying at least one C allele was associated with significantly increased mature miR-196a expression (p=0.006 or 0.002). Conclusions: Our results suggest, for the first time, that miR-196a2 polymorphism may contribute to cirrhosisrelated HCC susceptibility in Chinese patients through influencing mature miR-196a expression. © 2010 The Royal College of Pathologists of Australasia.
Shi Y.,Mudanjiang Medical University
ICSESS 2012 - Proceedings of 2012 IEEE 3rd International Conference on Software Engineering and Service Science | Year: 2012
Evaluation of campus network security is a complicated systematic project. The paper analyzes the reasons of the questions, obtains the solving method, and at last builds the M(1,2,3) model without the interference of redundant data, which is different from )M(g+) and is a nonlinear model. The paper will apply the new algorithm in the fuzzy evaluation on campus network security. © 2012 IEEE.
Song H.,Northeast Petroleum University |
Zhang Y.-J.,Northeast Petroleum University |
Song H.-L.,Mudanjiang Medical University |
Dai M.,Northeast Petroleum University
Ranliao Huaxue Xuebao/Journal of Fuel Chemistry and Technology | Year: 2012
The TiO2-Al2O3 complex support was prepared by the sol-gel method. A nickel phosphide catalyst, Ni2P/TiO2-Al2O3 with citric acid (CA) as chelating agent, was prepared by the impregnation method. The catalysts were characterized by X-ray diffraction (XRD), N2-adsorption specific surface area measurements (BET), thermogravimetry-differential thermal analysis (TG-DTA), temperature programmed reduction (TPR) and transmission electron microscope (TEM). The effects of different chelating agents and CA addition for dibenzothiophene (DBT) hydrodesulfurization (HDS) were studied. The result showed that the addition of appropriate amount of CA into the catalyst can enrich the pores of Ni2P/TiO2-Al2O3 catalyst, increase the surface area, which made better pore structure, higher dispersion of metal active component and more uniform size of the active component. CA can weaken the interaction between the active phase and the support, resulting in an apparent decrease in reduction temperature for nickel and phosphorus precursor as well as promotion of the formation of the Ni-P-O active phase. At reaction temperature of 360°C, pressure of 3.0 MPa, hydrogen/oil ratio of 500(volume ratio), liquid hourly space velocity of 2.0 h-1 and reaction time of 4 h, the initial dibenzothiophene conversion was 99.5% and stabilized at about 95.0% during 48 h reaction.
Li S.-O.,Mudanjiang Medical University |
Wang J.-L.,Mudanjiang Medical University |
Bjorklund G.,Council for Nutritional and Environmental Medicine |
Zhao W.-N.,Mudanjiang Medical University |
Yin C.-H.,Mudanjiang Medical University
NeuroReport | Year: 2014
Trace elements play a critical role in the pathogenesis of autism spectrum disorders (ASD). The aim of this study was to investigate the serum levels of zinc (Zn) and copper (Cu) in Chinese children with ASD. Sixty patients (48 males, 12 females) diagnosed with ASD and 60 healthy sex-matched and age-matched control participants were assessed for serum Zn and Cu content at admission. The severity of ASD was also evaluated using the Childhood Autism Rating Scale (CARS) score. The results indicated that the mean serum Zn levels and Zn/Cu ratio were significantly lower in children with ASD compared with normal cases (P<0.001, respectively), whereas serum Cu levels were significantly higher (P<0.001). There was a significant negative association between Zn/Cu and CARS scores (r=-0.345, P=0.007). On the basis of the receiver operating characteristic curve, the optimal cut-off value of serum levels of Zn/Cu as an indicator for an auxiliary diagnosis of autism was projected to be 0.665, which yielded a sensitivity of 90.0% and a specificity of 91.7%; the area under the curve was 0.968 (95% confidence interval, 0.943-0.993). In conclusion, these results suggested an association between serum levels of Zn and Cu and ASD among Chinese patients, and the Zn/Cu ratio could be considered a biomarker of ASD. © 2014 Wolters Kluwer Health | Lippincott Williams & Wilkins.