Zhu W.,Mudanjiang Medical College |
Nie Y.,Mudanjiang Medical College |
Shi X.,Mudanjiang Medical College |
Liu Y.,Mudanjiang Medical College |
And 2 more authors.
Immunological Investigations | Year: 2014
Ulcerative colitis, a major inflammatory bowel disease, is an idiopathic inflammatory disorder of the colonic mucosa, accompanied by an aberrant immune reaction to intestinal microflora. Macrophages are central mediators of intestinal immune homeostasis and inflammation. The relationship between macrophages and the pathogenesis of colitis is poorly understood. We aimed to characterize the changing populations and roles of M1/M2 macrophages in colitis. We demonstrated that M1 macrophages increased and M2 macrophages decreased in colitis, accompanied by Interleukin (IL)-23 and Tumor necrosis factor-α induction and IL-10 suppression. Transfer of M2 macrophages reduced dextran sodium sulfate-induced colitis by inducing IL-10 production and promoting regulatory T-cell generation. In vivo neutralization of IL-10 partially reduced the effects of M2 transfer. These findings suggest that macrophages play a critical role in colitis; specifically, disequilibrium of macrophage subsets promotes colitis development. A shift from the M1 to M2 phenotype reduces colitis by inducing IL-10; thus, mobilization of M2 macrophages could be a novel approach to colitis therapy. © 2014 Informa Healthcare USA, Inc.
Dong H.,Qiqihar Medical University |
Li R.,Mudanjiang Medical College |
Yu C.,Qiqihar Medical University |
Xu T.,Qiqihar Medical University |
And 2 more authors.
Neuroscience | Year: 2015
Parkinson's disease (PD) is second only to Alzheimer's disease as the most common devastating human neurodegenerative disorder. Despite intense investigation, no curative therapy is available for PD. Paeoniflorin, a monoterpene glucoside isolated from the Paeonia lactiflora Pall., possesses wide pharmacological effects in the nervous system. This study aims at evaluating the effect of paeoniflorin on 6-hydroxydopamine (6-OHDA)-induced apoptosis and to characterize involved signal transduction pathways in PC12 cells. Our results showed that paeoniflorin suppresses mitochondria-mediated apoptosis of PC12 cells induced by 6-OHDA, and anti-apoptotic effects of paeoniflorin on PC12 cells might mainly result from its antioxidant capability by increasing glutathione (GSH). Moreover, we also found that paeoniflorin can dramatically attenuate the 6-OHDA-induced nuclear factor κB (NF-κB) translocation without affecting phosphorylation of Akt, JNK, p38, and ERK1/2. 6-OHDA-induced protein kinase Cδ (PKCδ) upregulation was blocked by paeoniflorin treatment in PC12 cells. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor diphenyleneiodonium or NF-κB inhibitor BAY 11-7082 could partially attenuate 6-OHDA-induced cell death. Together, our results indicate that the inhibition of PC12 cell apoptosis by paeoniflorin might be mediated, at least in part, by inhibiting reactive oxygen species (ROS)/PKCδ/NF-κB signaling pathway. This evidence supports the pharmacological potential of paeoniflorin in the management of neurodegenerative disorders associated with oxidative stress, including PD. © 2014 IBRO.
Zhu L.,Guangdong University of Petrochemical Technology |
Gao J.,Mudanjiang Medical College |
An Z.,Guangdong University of Petrochemical Technology
Journal of Molecular Structure | Year: 2014
A new heterometallic compound, namely [CdCa(BPTC)(DMA)2(H 2O)]ṅn(DMA) (1 H4BPTC = biphenyl-3,3′,5,5′-tetracarboxylic acid, DMA = N,N′- dimethylacetamide), has been solvothermally synthesized by the reactions of Cd(NO3)2, Ca(NO3)2 and H 4BPTC. Single crystal X-ray analysis reveals that compound 1 features a complicated 3D framework based on dinuclear [CdCa(COO)3] subunits, which can be simplified into a 4-connected PtS topological network. In addition, the luminescent properties of compound 1 were also investigated in the solid state at room temperature. © 2014 Elsevier B.V. All rights reserved.
An Z.,Guangdong University of Petrochemical Technology |
Gao J.,Mudanjiang Medical College |
Zhu L.,Guangdong University of Petrochemical Technology
Journal of Molecular Structure | Year: 2013
Two new metal-organic frameworks, namely [Zn(nic)(mtz)]n (1) and [Zn(isonic)(mtz)]n (2) (Hnic = nicotinic acid, Hisonic = isonicotinic acid, Hmtz = 5-methyl-1H-tetrazole), have been obtained through the solvothermal reactions of Zn(NO3)2, Htmz and Hnic or Hisonic. Single crystal X-ray diffraction analysis reveals that compound 1 features a 2D layered structure with sql topology, which is further extended into a 3D supramolecular framework via weak CH.π interactions, and compound 2 is 2-fold interpenetrated 3D framework with dia topology. Luminescent investigation shows that both of them emit blue luminescence at room temperature. © 2013 Elsevier B.V. All rights reserved.
Lu Z.H.,University of Washington |
Shvartsman M.B.,University of Washington |
Lee A.Y.,University of Washington |
Shao J.M.,University of Washington |
And 7 more authors.
Cancer Research | Year: 2010
Small GTPase Ras homologue enriched in brain (RHEB) binds and activates the key metabolic regulator mTORC1, which has an important role in cancer cells, but the role of RHEB in cancer pathogenesis has not been shown. By performing a meta-analysis of published cancer cytogenetic and transcriptome databases, we defined a gain of chromosome 7q36.1-q36.3 containing the RHEB locus, an overexpression of RHEB mRNA in several different carcinoma histotypes, and an association between RHEB upregulation and poor prognosis in breast and head and neck cancers. To model gain of function in epithelial malignancy, we targeted Rheb expression to murine basal keratinocytes of transgenic mice at levels similar to those that occur in human squamous cancer cell lines. Juvenile transgenic epidermis displayed constitutive mTORC1 pathway activation, elevated cyclin D1 protein, and diffuse skin hyperplasia. Skin tumors subsequently developed with concomitant stromal angio-inflammatory foci, evidencing induction of an epidermal hypoxia-inducible factor-1 transcriptional program, and paracrine feed-forward activation of the interleukin-6-signal transducer and activator of transcription 3 pathway. Rheb-induced tumor persistence and neoplastic molecular alterations were mTORC1 dependent. Rheb markedly sensitized transgenic epidermis to squamous carcinoma induction following a single dose of Ras-activating carcinogen 7,12-dimethylbenz(a)anthracene. Our findings offer direct evidence that RHEB facilitates multistage carcinogenesis through induction of multiple oncogenic mechanisms, perhaps contributing to the poor prognosis of patients with cancers overexpressing RHEB. ©2010 AACR.
Song H.,Northeast Petroleum University |
Dai M.,Northeast Petroleum University |
Song H.,Mudanjiang Medical College
Progress in Chemistry | Year: 2012
More and more rigorous environmental regulations limiting the emissions of sulfur dioxide and the continuing decline in the quality of petroleum feedstocks have given rise to the need for investigation and development of high-performance hydrodesulfurization (HDS) catalysts. This paper provides a review of recent progress in the characteristics, active phase, synthesis, modification and HDS catalytic performance of Ni 2P catalyst. There are two types of initial active sites in Ni 2P. The Ni (1) initial active sites have a tetrahedral geometry, and which are involved in the direct desulfurization pathway in HDS. The Ni (2) initial active sites have a square pyramidal geometry, and which are responsible for the high catalytic activity in the hydrogenation pathway in HDS. A surface nickel phosphosulfide phase may be formed under HDS reaction, and which is considered as the real active phase. Ni 2P is mainly prepared by temperature-programmed reduction and liquid phase synthesis. The support, promoter and complex agent have important effect on the formation of Ni 2P active phase, as well as the catalytic activity. Compared with commercial sulfide catalyst, the Ni 2P catalyst shows higher HDS activity for thiophene, dibenzothiophene and 4, 6-dimethyldibenzothiophene.
Jia S.-J.,Central South University |
Niu P.-P.,Central South University |
Cong J.-Z.,Mudanjiang Medical College |
Zhang B.-K.,Central South University |
Zhao M.,Central South University
International Immunopharmacology | Year: 2014
Atherosclerosis has been widely considered as a chronic inflammation process, which triggers a wide range of cardiovascular diseases such as ischemic coronary artery disease (CAD). Toll-like receptor 4 (TLR4), a primary receptor of the innate immune system, plays a pivotal role in the initiation and progression of atherosclerosis. Here we summarize recent progress on understanding the activation and function of TLR4 signaling in the initiation and development of CAD, with the focus on the role of TLR4 as a link between CAD and other inflammatory diseases. Furthermore, we list a variety of drugs which exert anti-atherosclerosis effects via targeting TLR4 signaling. Finally, we discuss the promise of TLR4 signaling as a therapeutic target for CAD. © 2014 Elsevier B.V.
Zhao F.,Mudanjiang Medical College
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery | Year: 2011
Bone marrow mesenchymal stem cells (BMSCs) are multipotent and thus are able to differentiate into a number of different cell types under certain culture condition. However, the effect of age on the differentiation remains unknown. To explore the effect of the microenvironment formed by Schwann cells (SCs) on BMSCs differentiation into neurons and oligodendrocytes in rats at different ages in vitro. SCs were extracted and purified from the distal sciatic nerves of neonatal Wistar rats. BMSCs were isolated from bone marrow of Wistar rats (aged 1 month, 6 months, and 12 months, respectively) and cultured in vitro. The cells were identified by immunofluorescent staining. The BMSCs at passage 2 were labeled by PKH26 and cocultured with SCs at passage 3 in equal proportions in two layer Petri dish. According to the BMSCs from the rats at different ages, experiment was divided into 3 groups: SCs were cocultured with 1-month-old rat BMSCs (group A), 6-month-old rat BMSCs (group B), and 12-month-old rat BMSCs (group C), respectively. The morphological changes of cocultured BMSCs were observed by inverted phase contrast microscope, the expressions of neuron-specific enolase (NSE) and myelin basic protein (MBP) in the cocultured BMSCs were tested by immunofluorescent staining, and the expression of neuregulin 1 (NRG1) was detected by ELISA method. SCs and BMSCs were isolated and cultured successfully. The identification of SCs showed positive expression of S-100 and BMSCs showed positive expressions of CD29, CD44, and CD90. At 7 days after coculture, the BMSCs in group A began retraction, and became round or tapered with the processes and had a nerve cells or oligodendrocytes-like morphology, but most BMSCs in groups B and C showed no obvious morphological changes under inverted phase contrast microscope. Immunofluorescent staining showed that the positive expression rates of NSE in groups A, B, and C were 22.39% +/- 2.86%, 12.89% +/- 1.78%, and 2.69% +/- 0.80%, respectively, and the positive expression rates of MBP in groups A, B, and C were 16.13% +/- 2.39%, 6.33% +/- 1.40%, and 0.92% +/- 0.17%, respectively. There were significant differences in terms of NSE and MBP positive expression rates among 3 groups (P < 0.05). ELISA analysis showed that NRG1 in the supernatant of group A was increased after coculture in a time-dependent manner. At 6, 9, and 12 days of coculture, NRG1 content was higher in group A than in groups B and C, and in group B than in group C, showing significant differences (P < 0.05). The microenvironment formed by SCs can promote BMSCs differentiation into neurons and oligodendrocytes, but the differentiation capability of BMSCs decreases with aging, and the variety of growth factors secreted by SCs is likely important factors that induce the differentiation of BMSCs into neurons and oligodendrocytes.
Li X.,Mudanjiang Medical College
Chinese Journal of Biologicals | Year: 2013
Objective: To construct and primarily screen the Fab phage display library against Sendai virus Tianjin strain. Methods: Total RNA was extracted from spleen cells of mice vaccinated with Sendai virus Tianjin strain, with which antibody light chain (κ) and heavy chain (Fd) genes were amplified by RT-PCR. The κ gene and phagemid p3MH were digested with Sac I and Xba I, then purified, recovered, linked with T4 DNA ligase, and transformed to E. coli XL1-Blue by electroporation. The constructed light chain phage display library p3MH-κ and Fd gene were digested with Spe I and Xho I, then purified, recovered and linked, and transformed to E. coli XL1-Blue. The constructed phage antibody library was calculated for recombination rate and titer, and screened primarily using the recombinant protein HN2 of Sendai virus Tianjin strain as an antigen, and the obtained phage antibody was sequenced. Results: The Fab phage antibody library against Sendai virus Tianjin strain, with a capacity of 1.18 × 107, was constructed, of which the recombination rate was 90%, and the titer of prepared phage antibody was 1011 pfu/ml. Primary screening proved that the phages were enriched by about 63.6 folds. Two positive clones with binding activities to recombinant HN2 protein were obtained, and proved as murine antibody by analysis of homology using NCBI BLAST software. IMGT analysis showed that the homology of light chain gene to V κ9 subgroup gene was 94.87%, while that of heavy chain gene to VH7 subgroup gene was 97.85%. Conclusion: The Fab phage display library against Sendai virus Tianjin strain was successfully constructed, which laid a foundation of diagnosis of the strain, therapy of relevant disease as well as study on pathogenic mechanism.
Ma Y.-B.,Mudanjiang Medical College
Chinese Journal of Tissue Engineering Research | Year: 2014
BACKGROUND: The treatment options of intertrochanteric fracture in elderly patients are very difficult, internal fixation and arthroplasty have their own advantages and disadvantages. The clinical efficacy of internal fixation and arthroplasty has been compared, and total hip replacement is the preferred choice for elderly patients with intertrochanteric fractures. OBJECTIVE: To compare the therapeutic effect and complications of total hip replacement and proximal femoral nail anti-rotation in elderly patients with intertrochanteric fracture. METHODS: 67 elderly patients with intertrochanteric fracture were selected from Second Department of Orthopedics, Hongqi Hospital of Mudanjiang Medical College, aged 65-87 years. According to the different treatment options, the involved patients were divided into total hip replacement group (n=31) and proximal femoral nail anti-rotation group (n=36). Conventional fixation approach was performed in the two groups. RESULTS AND CONCLUSION: The open reduction and internal fixation were successfully performed in 67 elderly patients with intertrochanteric fractures. Compared with proximal femoral nail anti-rotation group, the intraoperative blood loss was increased, Harris scores and visual analog scores were obviously improved in the total hip replacement group (P < 0.05); the postoperative bed time, medical complications and hip deformity were reduced (P < 0.05). Total hip replacement is more effective and safer than proximal femoral nail anti-rotation in treatment of intertrochanteric fracture among elderly patients. © 2014, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.