Mudanjiang Branch of Heilongjiang Academy of Agricultural science

Mudanjiang, China

Mudanjiang Branch of Heilongjiang Academy of Agricultural science

Mudanjiang, China
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Yu W.,Mudanjiang Branch of Heilongjiang Academy of Agricultural science | Guo J.,Dongning Fruit and Vegetable Management Station | Gu G.,Mudanjiang Branch of Heilongjiang Academy of Agricultural science | Bu H.,Mudanjiang Branch of Heilongjiang Academy of Agricultural science | And 7 more authors.
Acta Horticulturae Sinica | Year: 2017

'Longshuai' is a new apple cultivar derived from the hybrid crossing 'Hanfu × 'Jinhong' which is high quality, high yield and cold resistant. The fruit is oval in shape. The average fruit weight is 119 g. The fruit having a thin yellow ground skin, covered with red, striped red, and the surface is smooth. The flesh is light yellow, tender and crunchy texture, medium-juice, refreshing and fragrant, sour-sweet. The soluble solids content is 13.9%, the soluble sugar content is 12.75%, the titratable acid content is 5.62 g . kg-1 and the vitamin C content is 128.2 mg . kg-1, with a very good eating quality. The cultivar is suitable for the southeastern of Heilongjiang and the most parts of Jilin Province.


Fang C.,CAS Institute of Genetics and Developmental Biology | Fang C.,University of Chinese Academy of Sciences | Ma Y.,CAS Institute of Genetics and Developmental Biology | Wu S.,CAS Institute of Genetics and Developmental Biology | And 35 more authors.
Genome Biology | Year: 2017

Background: Soybean (Glycine max [L.] Merr.) is one of the most important oil and protein crops. Ever-increasing soybean consumption necessitates the improvement of varieties for more efficient production. However, both correlations among different traits and genetic interactions among genes that affect a single trait pose a challenge to soybean breeding. Results: To understand the genetic networks underlying phenotypic correlations, we collected 809 soybean accessions worldwide and phenotyped them for two years at three locations for 84 agronomic traits. Genome-wide association studies identified 245 significant genetic loci, among which 95 genetically interacted with other loci. We determined that 14 oil synthesis-related genes are responsible for fatty acid accumulation in soybean and function in line with an additive model. Network analyses demonstrated that 51 traits could be linked through the linkage disequilibrium of 115 associated loci and these links reflect phenotypic correlations. We revealed that 23 loci, including the known Dt1, E2, E1, Ln, Dt2, Fan, and Fap loci, as well as 16 undefined associated loci, have pleiotropic effects on different traits. Conclusions: This study provides insights into the genetic correlation among complex traits and will facilitate future soybean functional studies and breeding through molecular design. © 2017 The Author(s).


Duan N.,Shandong Agricultural University | Duan N.,Shandong Academy of Agricultural Sciences | Bai Y.,Cornell University | Sun H.,Cornell University | And 40 more authors.
Nature Communications | Year: 2017

Human selection has reshaped crop genomes. Here we report an apple genome variation map generated through genome sequencing of 117 diverse accessions. A comprehensive model of apple speciation and domestication along the Silk Road is proposed based on evidence from diverse genomic analyses. Cultivated apples likely originate from Malus sieversii in Kazakhstan, followed by intensive introgressions from M. sylvestris. M. sieversii in Xinjiang of China turns out to be an "ancient" isolated ecotype not directly contributing to apple domestication. We have identified selective sweeps underlying quantitative trait loci/genes of important fruit quality traits including fruit texture and flavor, and provide evidences supporting a model of apple fruit size evolution comprising two major events with one occurring prior to domestication and the other during domestication. This study outlines the genetic basis of apple domestication and evolution, and provides valuable information for facilitating marker-Assisted breeding and apple improvement. © 2017 The Author(s).


Jiang B.,Chinese Academy of Agricultural Sciences | Yue Y.,Chinese Academy of Agricultural Sciences | Yue Y.,Mudanjiang Branch of Heilongjiang Academy of Agricultural science | Gao Y.,Chinese Academy of Agricultural Sciences | And 6 more authors.
PLoS ONE | Year: 2013

Background:Soybean is a short-day crop of agricultural, ecological, and economic importance. The sensitive photoperiod responses significantly limit its breeding and adaptation. GmFT2a, a putative florigen gene with different transcription profiles in two cultivars (late-maturing Zigongdongdou and early-maturing Heihe 27) with different maturity profiles, is key to flowering and maturation. However, up to now, its role in the diverse patterns of maturation in soybeans has been poorly understood.Methods:Eighty varieties, including 19 wild accessions, covering 11 of all 13 maturity groups, were collected. They were planted in pots and maintained under different photoperiodicity conditions (SD, short day; LD, long day; and ND, natural day). The day to first flowering was recorded and the sensitivity to photoperiod was investigated. Polymorphisms in the GmFT2a coding sequence were explored by searching the known SNP database (NCBI dbSNP). The GmFT2a promoter regions were then cloned from these varieties and sequenced. Further polymorphism and association analyses were conducted.Results:These varieties varied greatly in time to first flowering under ND and exhibited a consecutive distribution of photoperiod sensitivity, which suggested that there is rich diversity in flowering time. Furthermore, although GmFT2a had only one known synonymous SNP in the coding sequence, there were 17 haplotypes of the GmFT2a promoter region, HT06 of which was extremely abundant. Further association analysis found some SNPs that might be associated with day to first flowering and photoperiod sensitivity.Conclusion:Although GmFT2a is a key flowering gene, GmFT2a polymorphism does not appear to be responsible for maturity diversity in soybean. © 2013 Jiang et al.


Lu S.,Chinese Academy of Sciences | Lu S.,University of Chinese Academy of Sciences | Li Y.,Northeast Forestry University | Wang J.,Chinese Academy of Sciences | And 17 more authors.
Euphytica | Year: 2015

Flowering represents the transition from the vegetative to reproductive phase and plays an important role in many agronomic traits. For soybean, a short day (SD) induced and photoperiod-sensitive plant, delaying flowering time under SD environments is very important and has been used by breeders to increase yields and enhance plant adaptabilities at lower latitudes. The purpose of this study was to identify quantitative trait loci (QTLs) associated with flowering time, especially QTLs underlying the long juvenile (LJ) trait which delays flowering time under SD environments. A population of 91 recombinant inbred lines derived from a cross between AGS292 and K3 was used for map construction and QTL analysis. The map covered 2546.7 cM and included 52 new promoter-specific indel and 9 new exon-specific indel markers. The phenotypic days-to-flowering data were examined in nine environments, including four short-day (SD, low latitude) and five long-day photoperiod (LD, high latitude) environments. For the SD environments, six QTLs were detected. Five of them were associated with the LJ trait. Among the five LJ QTLs, four QTLs may be attributed to the known flowering time genes, including qFT-J-1 for FT5a locus, qFT-J-2 for the FT2a locus, qFT-O for the E2 locus and qFT-L for the E3 locus. This is the first report that the E2, E3, FT2a and FT5a loci may be associated with the LJ trait. Under the five LD environments, as expected, qFT-O for the E2 locus and qFT-L for the E3 locus were identified, suggesting that E2 and E3 loci are very important for soybean adaptation in LD photoperiod. Conjoint analysis of multiple environments identified nine additive QTLs and nine pairs of epistatic QTLs, among which most were involved in interactions with the environments. In total, five QTLs (qFT-B2-1, qFT-C1-1, qFT-K, qFT-D2 and qFT-F) were identified that may represent novel flowering time genes. This provides a fundamental foundation for future studies of flowering time in soybean using fine mapping, map-based cloning, and molecular-assisted breeding. © 2015 Springer Science+Business Media Dordrecht


Man L.,Hei Long Jiang Agricultural economics Vocational College | Xiang D.,Hei Long Jiang Agricultural economics Vocational College | Wang L.,Daqing Branch of Heilongjiang Academy of Agricultural science | Zhang W.,Mudanjiang Branch of Heilongjiang Academy of Agricultural science | And 2 more authors.
Protoplasma | Year: 2016

The ICE1 transcription factor plays a critical role in plant cold tolerance via triggering CBF/DREB1 cold-regulated signal networks. In this work, a novel MYC-type ICE1-like gene, RsICE1, was isolated from radish (Raphanus sativus L.), and its function in cold tolerance was characterized in rice. The RsICE1 gene was expressed constitutively with higher transcriptional levels in the roots and stems of radish seedlings. The NaCl, cold, and ABA treatments could significantly upregulate RsICE1 expression levels, but dehydration stress had a weak effect on its expression. Ectopic expression of the RsICE1 gene in rice conferred enhanced tolerance to low-temperature stress grounded on a higher survival rate, higher accumulation of soluble sugars and free proline content, a decline in electrolyte leakage and MDA levels, and higher chlorophyll levels relative to control plants. OsDREBL and OsTPP1, downstream cold-regulated genes, were remarkably upregulated at transcription levels in rice overexpressing RsICE1 under low-temperature stress, which indicated that RsICE1 was involved in CBF/DREB1 cold-regulated signal networks. Overall, the above data showed that RsICE1 played an active role in improving rice cold tolerance, most likely resulting from the upregulation of OsDREBL and OsTPP1 expression levels by interacting with the RsICE1 gene under low-temperature stress. © 2016 Springer-Verlag Wien


Liu D.,ShenYang Agricultural University | Liu D.,Mudanjiang Branch of Heilongjiang Academy of Agricultural science | Wang J.-Y.,ShenYang Agricultural University | Wang X.-X.,ShenYang Agricultural University | And 3 more authors.
Journal of Integrative Agriculture | Year: 2015

Abundant genetic diversity and rational population structure of germplasm benefit crop breeding greatly. To investigate genetic variation among geographically diverse set of japonica germplasm, we analyzed 233 japonica rice cultivars collected from Liaoning, Jilin and Heilongjiang provinces of China, which were released from 1970 to 2011 by using 62 simple sequence repeat (SSR) markers and 8 functional gene tags related to yield. A total of 195 alleles (Na) were detected with an average of 3.61 per locus, indicating a low level of genetic diversity level among all individuals. The genetic diversity of the cultivars from Jilin Province was the highest among the three geographic distribution zones. Moreover, the genetic diversity was increased slightly with the released period of cultivars from 1970 to 2011. The analysis of molecular variance (AMOVA) revealed that genetic differentiation was more diverse within the populations than that among the populations. The neighbor-joining (NJ) tree indicated that cultivar clusters based on geographic distribution represented three independent groups, among which the cluster of cultivars from Heilongjiang is distinctly different to the cluster of cultivars from Liaoning. For the examined functional genes, two or three allelic variations for each were detected, except for IPA1 and GW2, and most of elite genes had been introgressed in modern japonica rice varieties. These results provide a valuable evaluation for genetic backgrounds of current japonica rice and will be used directly for japonica rice breeding in future. © 2015 Chinese Academy of Agricultural Sciences.


Guo S.-J.,Shanxi Agricultural University | Yang K.-M.,Shanxi Agricultural University | Huo J.,Shanxi Agricultural University | Zhou Y.-H.,Shanxi Agricultural University | And 2 more authors.
Chinese Journal of Applied Ecology | Year: 2015

A drought-resistant soybean cultivar Jinda 70 and a drought-sensitive soybean cultivar Jindou 26 were taken as test materials. At the grain filling stage, the cultivars were subject to three water treatments including sufficient water supply, light drought stress, and severe drought stress by using pot experiments for research on influence of drought on leaf photosynthetic capacity and root growth of soybeans. The results showed that as the degree of drought stress was aggravated, all of the indices including leaf area, chlorophyll content, net photosynthetic rates (Pn), stomatal conductance (gs), transpiration rate (Tr), intercellular CO2 concentration (Ci), plant mass, plant height, seed yield, and harvest index in the two cultivars declined. The root length and root mass increased under light drought stress, and decreased under severe drought stress. Root-shoot ratio ascended as the degree of drought stress was aggravated. Under severe drought stress, the increase of root-shoot ratio of the drought-resistant soybean cultivar Jinda 70 was up to 135.7%, which was higher than the that (116.7%) of the drought-sensitive soybean cultivar Jindou 26. Simultaneously, leaf area and chlorophyll content in Jinda 70 were respectively 69.3% and 85.5% of those in the control, which were better than those of Jindou 26. gs and Pn of Jinda 70 respectively declined 67.9% and 77.9%, but still lower than those of Jindou 26. Therefore, the decline range of harvest index of Jinda 70 was 43.8%, which was lower than the range of 78.8% of Jindou 26. The Biplot revealed that under different dry treatments, there were significant positive correlations among the sixindexes including leaf area, chlorophyll content, Pn, gs, Tr, and Ci of the two cultivars. There were also significant positive correlations among the six indices including plant mass, plant height, root length, root mass, seed yield, and harvest index. Root-shoot ratio only had significant positive correlation with root mass and had significant negative correlations with other five indices. ©, 2015, Editorial Board of Chinese Journal of Applied Ecology. All right reserved.


Zhai H.,Chinese Academy of Sciences | Lu S.,Chinese Academy of Sciences | Lu S.,University of Chinese Academy of Sciences | Wang Y.,Jilin Agricultural University | And 12 more authors.
PLoS ONE | Year: 2014

The time to flowering and maturity are ecologically and agronomically important traits for soybean landrace and cultivar adaptation. As a typical short-day crop, long day conditions in the high-latitude regions require soybean cultivars with photoperiod insensitivity that can mature before frost. Although the molecular basis of four major E loci (E1 to E4) have been deciphered, it is not quite clear whether, or to what degree, genetic variation and the expression level of the four E genes are associated with the time to flowering and maturity of soybean cultivars. In this study, we genotyped 180 cultivars at E1 to E4 genes, meanwhile, the time to flowering and maturity of those cultivars were investigated at six geographic locations in China from 2011 to 2012 and further confirmed in 2013. The percentages of recessive alleles at E1, E2, E3 and E4 loci were 38.34%, 84.45%, 36.33%, and 7.20%, respectively. Statistical analysis showed that allelic variations at each of four loci had a significant effect on flowering time as well as maturity. We classified the 180 cultivars into eight genotypic groups based on allelic variations of the four major E loci. The genetic group of e1-nf representing dysfunctional alleles at the E1 locus flowered earliest in all the geographic locations. In contrast, cultivars in the E1E2E3E4 group originated from the southern areas flowered very late or did not flower before frost at high latitude locations. The transcriptional abundance of functional E1 gene was significantly associated with flowering time. However, the ranges of time to flowering and maturity were quite large within some genotypic groups, implying the presence of some other unknown genetic factors that are involved in control of flowering time or maturity. Known genes (e.g. E3 and E4) and other unknown factors may function, at least partially, through regulation of the expression of the E1 gene. © 2014 Zhai et al.


Xiang D.,Hei Long Jiang Agricultural economics Vocational College | Chai Y.,Mudanjiang Branch of Heilongjiang Academy of Agricultural science | Man L.,Hei Long Jiang Agricultural economics Vocational College | Sun Y.,Mudanjiang Branch of Heilongjiang Academy of Agricultural science | And 9 more authors.
Plant Cell, Tissue and Organ Culture | Year: 2016

Low temperature stress operates the ICE1-CBF cold-responsive signal pathway of plants, which leads to the overexpression of a series of genes to enhance freezing tolerance. In this study, a novel MYC-type ICE1-like gene, BcICE1, was isolated from heading Chinese cabbage (Brassica campestris ssp. Pekinensis Lour. Olsson), and its function in freezing tolerance was characterized in rice. The expression of BcICE1 gene was constitutive with higher transcriptional levels in stems and leaves than in roots. The low temperature, ABA and NaCl treatments could significantly up-regulate BcICE1 expression levels, but dehydration stress had less effect on its expression. Under low temperature stress, the rice lines overexpressing BcICE1 gene enhanced freezing tolerance according to higher survival rate, higher accumulation of soluble sugars and proline, a remarkable decline in electrolyte leakage rate and MDA levels, and higher chlorophyll content compared with non-transgenic plants. As downstream cold-regulated genes, the expression levels of OsDREB1B, OsTPP1, J013091D15 and J023041L05 were obviously up-regulated in rice overexpressing BcICE1 under low temperature stress, suggesting BcICE1 was dependent of a CBF/DREB1 cold-responsive pathway. In short, above data showed that BcICE1 had a positive effect on improving rice freezing tolerance, which most likely result from the up-regulation of OsDREB1B, OsTPP1, J013091D15 and J023041L05 expression levels by interaction with the BcICE1 gene under low temperature stress. © 2016 Springer Science+Business Media Dordrecht

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