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Salilew-Wondim D.,University of Bonn | Holker M.,University of Bonn | Rings F.,University of Bonn | Ghanem N.,University of Bonn | And 6 more authors.
Physiological Genomics | Year: 2010

Aberrant gene expression in the uterine endometrium and embryo has been the major causes of pregnancy failure in cattle. However, selecting cows having adequate endometrial receptivity and embryos of better developmental competence based on the gene expression pattern has been a greater challenge. To investigate whether pretransfer endometrial and embryo gene expression pattern has a direct relation with upcoming pregnancy success, we performed a global endometrial and embryo transcriptome analysis using endometrial and embryo biopsy technology and the pregnancy outcome information. For this, endometrial samples were collected from Simmental heifers at day 7 and 14 of the estrous cycle, one cycle prior to embryo transfer. In the next cycle, blastocyst stage embryos were transferred to recipients at day 7 of the estrous cycle after taking 30-40% of the blastocyst as a biopsy for transcriptome analysis. The results revealed that at day 7 of the estrous cycle, the endometrial gene expression pattern of heifers whose pregnancy resulting in calf delivery was significantly different compared with those resulting in no pregnancy. These differences were accompanied by qualitative and quantitative alteration of major biological process and molecular pathways. However, the transcriptome difference was minimal between the two groups of animals at day 14 of the estrous cycle. Similarly, the transcriptome analysis between embryos biopsies that resulted in calf delivery and those resulted in no pregnancy revealed a total of 70 differentially expressed genes. Among these, the transcript levels of 32 genes including SPAG17, PF6, UBE2D3P, DFNB31, AMD1, DTNBP1, and ARL8B were higher in embryo biopsies resulting in calf delivery. Therefore, the present study highlights the potential of pretransfer endometrial and embryo gene expression patterns as predictors of pregnancy success in cattle. Copyright © 2010 the American Physiological Society. Source


Torino M.I.,CONICET | Limon R.I.,Institute Ciencia Y Tecnologia Of Alimentos Y Nutricion Ictan Csic | Martinez-Villaluenga C.,Institute Ciencia Y Tecnologia Of Alimentos Y Nutricion Ictan Csic | Makinen S.,MTT Biotechnology and Food Research | And 3 more authors.
Food Chemistry | Year: 2013

The effect of liquid (LSF) and solid state fermentation (SSF) of lentils for production of water-soluble fractions with antioxidant and antihypertensive properties was studied. LSF was performed either spontaneously (NF) or by Lactobacillus plantarum (LP) while SSF was performed by Bacillus subtilis (BS). Native lactic flora in NF adapted better than L. plantarum to fermentative broth and BS counts increased 4.0 log CFU/g up to 48 h of SSF. LSF water-soluble fractions had higher (P ≤ 0.05) free amino groups, GABA content, antioxidant and angiotensin I-converting enzyme inhibitory (ACEI) activities than SSF. In addition, GABA and ACEI activity of LSF increased in a time-dependent manner. Proteolysis by BS was limited, with slight changes in free amino groups, while GABA, total phenolic compounds and antioxidant capacity increased throughout fermentation. Higher antihypertensive potential was observed in NF (96 h) characterised by the highest GABA content (10.42 mg/g extract), ACE-inhibitory potency (expressed as IC50) of 0.18 mg protein/ml and antioxidant capacity of 0.26 mmol Trolox equivalents/g extract. Therefore, water-soluble fermented lentil extracts obtained by LSF are particularly promising as functional ingredients in preventing hypertension. © 2012 Elsevier Ltd. All rights reserved. Source


Honkatukia M.,MTT Biotechnology and Food Research | Tuiskula-Haavisto M.,MTT Biotechnology and Food Research | Arango J.,Dallas Center | Tabell J.,MTT Biotechnology and Food Research | And 3 more authors.
Genetics Selection Evolution | Year: 2013

Background: A fresh, good quality egg has a firm and gelatinous albumen that anchors the yolk and restricts growth of microbiological pathogens. As the egg ages, the gel-like structure collapses, resulting in thin and runny albumen. Occasionally thin albumen is found in a fresh egg, giving the impression of a low quality product. A mapping population consisting of 1599 F2 hens from a cross between White Rock and Rhode Island Red lines was set up, to identify loci controlling albumen quality. The phenotype for albumen quality was evaluated by albumen height and in Haugh units (HU) measured on three consecutive eggs from each F2 hen at the age of 40 weeks. For the fine-mapping analysis, albumen height and HU were used simultaneously to eliminate contribution of the egg size to the phenotype. Results: Linkage analysis in a small population of seven half-sib families (668 F2) with 162 microsatellite markers spread across 27 chromosomes revealed two genome-wide significant regions with additive effects for HU on chromosomes 7 and Z. In addition, two putative genome-wide quantitative trait loci (QTL) regions were identified on chromosomes 4 and 26. The QTL effects ranged from 2 to 4% of the phenotypic variance. The genome-wide significant QTL regions on chromosomes 7 and Z were selected for fine-mapping in the full set composed of 16 half-sib families. In addition, their existence was confirmed by an association analysis in an independent commercial Hy-Line pure line. Conclusions: We identified four chicken genomic regions that affect albumen quality. Our results also suggest that genes that affect albumen quality act both directly and indirectly through several different mechanisms. For instance, the QTL regions on both fine-mapped chromosomes 7 and Z overlapped with a previously reported QTL for eggshell quality, indicating that eggshell membranes may play a role in albumen quality. © 2013 Honkatukia et al.; licensee BioMed Central Ltd. Source


Pihlanto A.,MTT Biotechnology and Food Research | Johansson T.,MTT Biotechnology and Food Research | Makinen S.,MTT Biotechnology and Food Research
Engineering in Life Sciences | Year: 2012

This study investigates the inhibition of angiotensin I-converting enzyme (ACE) and lipid peroxidation of fermented rapeseed and flaxseed meals. Rapeseed and flaxseed meals were fermented with Lactobacillus helveticus, Lactococcus lactis ssp. lactis, Bacillus subtilis, and Kluyveromyces marxianus monocultures at 37°C or 30°C for 48 h. The ACE-inhibition potencies increased by 20-37% during fermentation with L. helveticus and B. subtilis, whereas fermentation with other strains did not produce a significant change in ACE inhibition. The lowest IC50 value of 0.36 mg/mL was found with flaxseed fermented by L. helveticus. The same strains were also able to produce compounds that inhibited lipid peroxidation. The results showed that L. helveticus and B. subtilis degraded effectively rapeseed and flaxseed proteins as the amount of low molecular weight compounds increased during fermentation. ACE inhibition was found in low molecular weight (300-500 Da) fractions. In fermented rapeseed, the inhibition of lipid peroxidation was found in 1200-1300 Da fractions and fermented flaxseed showed inhibition in several fractions, first around 1300 Da and at lower molecular weight fractions 300-500 Da. The results demonstrated that fermentation with L. helveticus or B. subtilis produce compounds that may have beneficial effect on cardiovascular health. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source


Kauppinen T.,MTT Biotechnology and Food Research | Pesonen I.,MTT Biotechnology and Food Research | Katajajuuri J.-M.,MTT Biotechnology and Food Research | Kurppa S.,MTT Biotechnology and Food Research
Progress in Industrial Ecology | Year: 2010

Consumers play an important role in the food chain environmental system. However, there seem to be only few studies that have focused on food-related environmental impacts of consumer activities. This paper identifies the primary consumer activities that affect carbon footprints and their relative importance, and consumer choice in household food-related transportation, preservation and preparation. The data for food transportation, preservation and preparation were collected from existing surveys from which the information has been adapted to households. According to the study results, a Finnish household on average produces 170 kilograms of CO2-equivalent per capita, of which 50% derives from food preservation, 27% from food transportation and 23% from food preparation. Copyright © 2010 Inderscience Enterprises Ltd. Source

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