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Reddy S.S.,Msramaiah Dental College And Hospital
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2013

Determination of nicotine metabolism and pharmacokinetics provides a useful tool for estimating uptake of nicotine and tobacco related toxins, for understanding the pharmacologic effects of nicotine and nicotine addiction, and for optimizing nicotine dependency treatment during clinical encounter to improve patient compliance. Validation of self reported tobacco use status has significant clinical importance in cessation clinics, pulmonary and pediatric clinics and research settings. To estimate the serum nicotine levels among tobacco users. To understand the intake of nicotine and nicotine addiction for optimizing nicotine dependency treatment. 60 Healthy male volunteers between the age group of 18-45yrs using tobacco in either smoking or smokeless form for more than 3 months, 5 times a day were selected for the study. The smoking form consisted of use of cigarettes and bidis and smokeless form consisted of use of commercially available chewing tobacco sachets (pan masala etc). 3 blood samples were collected from each volunteer , 1st sample was collected in the early morning(first session), 30 minutes after the use of first tobacco product, the 2nd sample 60 min later, and the 3rd sample 90 min later. Serum nicotine levels were then assessed using Chemiluminescent immunoassay technique. ANOVA test was used to compare mean nicotine levels among all three groups and Post Hoc test was also undertaken to assess the difference in mean nicotine levels between two groups at a time. Average serum nicotine concentration among cigarette smokers at the end of 30 minutes was180(110-320)ngs\ml, at 60 minutes 40(30-50)ngs\ml and at 90 minutes 10(0-20)ngs\ml , among bidi smokers at the end of 30 minutes, it was 260(240-450) ngs\ml, at 60 minutes 70(50-90)ngs\ml and at 90 minutes 10(10-30)ngs\ml; among tobacco chewers at the end of 30 minutes it was 140(90-200)ngs\ml, at 60 minutes 50(40-60)ngs\ml and at 90 minutes 30(10-40)ngs\ml. Serum nicotine levels among cigarette and bidi smokers was significantly high compared to tobacco chewers. High serum nicotine levels among smokers could be attributed to high nicotine content of the tobacco product, method of consumption, rate of nicotine absorption and elimination. These findings suggest different strategies for treatment of tobacco dependence in these subjects as these three kinds of tobacco users would have different therapeutic needs. Source


Shukla D.,Jamia Millia Islamia University | Vinuth D.P.,Hitkarni Dental College and Hospital | Sowmya S.V.,Msramaiah Dental College And Hospital | Jeevan M.B.,Vydehi Institute of Dental science and Research Center | And 2 more authors.
Journal of Forensic Odonto-Stomatology | Year: 2012

Dental cementum is a specialized calcified structure covering the root of a tooth. This study aims to investigate cementum using various stains which can be exceedingly useful in investigation, observation and diagnosis. 4!m sections of 25 extracted normal teeth, 25 cases of various cemental pathologies and 25 ground sections were stained using cresyl violet, H&E, toluidine blue and periodic acid Schiff and were observed under light and florescence microscopes. Cresyl violet showed best contrast amongst all stains in decalcified and ground sections under light and florescence microscopy. Under the fluorescence microscope, cementum floresced more distinctly than dentin and enamel. Among the cemental pathologies examined, osteoid and cementoid exhibited florescence but cementum and bone did not fluoresce. Incremental lines were prominently visualised with cresyl violet under fluorescent microscopy, which may aid in forensic determination of age. The present results demonstrate that cementum in normal decalcified teeth and cemento-osseous lesions, could be observed best using cresyl violet stain under florescence microscopy. Source


Chippagiri P.,Msramaiah Dental College And Hospital | Banavar S.R.,Msramaiah Dental College And Hospital
Journal of Clinical and Diagnostic Research | Year: 2014

Context: Oral squamous cell carcinoma (OSCC) is the most common cancer of the head and neck. It accounts for more than 90% of all malignancies Which occur in the head and neck region. The high incidence and mortality rate of oral cancer stimulates continuation of research on finding new diagnostic tools or markers for it. Aim: To evaluate the possible role of serum β-carotene as a biochemical parameter in the diagnosis of oral squamous cell carcinoma.Materials and Methods: The serum β-carotene levels in 40 patients with clinically diagnosed and histopathologically confirmed oral squamous cell carcinoma were estimated and the levels were compared with those of 40 healthy controls. The serum β-carotene levels were estimated by using a spectrophotometer. The data which was obtained was analyzed statistically by using unpaired t-test.Results: Subjects with oral squamous cell carcinoma showed significantly lower levels of mean serum β-carotene (149.95 ± 61.29) as compared to those seen in controls (278.19 ± 90.12).Conclusion: The results of the present study are encouraging and they suggest that the estimation of the low levels of β-carotene in the patients with oral squamous cell carcinoma may be a useful diagnostic tool for making the diagnosis of oral squamous Cell carcinoma and thereby improving the prognosis of this dreaded disease. Source


Shwetha V.,Msramaiah Dental College And Hospital | Yashoda Devi B.K.,Msramaiah Dental College And Hospital | Mysorekar V.V.,Ms Ramaiah Medical College | Kamath N.P.,Msramaiah Dental College And Hospital
Journal of Clinical and Diagnostic Research | Year: 2014

Sjogren's syndrome (SS) is a chronic autoimmune disorder which is characterized by lymphocyte-mediated destruction of exocrine glands, which produces the classical symptoms of dry eyes and dry mouth, which is referred to as primary SS or the Sicca complex. When it is associated with another autoimmune disease such as rheumatoid arthritis or lupus erythematosus, the condition is termed as secondary SS. One of the known major complications in patients with Sjogren's syndrome is the occurrence of Non-Hodgkin's lymphoma of B cell type. It is not uncommon for malignant lymphomas to occur in head and neck region at nodal and sometimes, extranodal sites. However, only rarely may they involve the oral cavity primarily. This case report describes a rare occurrence of isolated extranodal lymphomas in the upper and lower lips of a patient, which clinically resembled a mucocele, and eventually was diagnosed as lymphoma which was associated with Sjogren's syndrome, thereby stressing the importance which was played by an oral diagnostician. Source


Prasad K.,Msramaiah Dental College And Hospital | Rau A.,Msramaiah Medical College And Hospital | Muniyappa S.,Msramaiah Medical College And Hospital | Kumar N.,Msramaiah Dental College And Hospital
Oral Surgery | Year: 2015

Myoepithelial carcinoma, also known as malignant myoepithelioma, is a rare locally aggressive primary salivary gland neoplasm. It is composed almost exclusively of neoplastic cells with myoepithelial differentiation and characterised by infiltrative growth and potential for distant metastasis. There is limited data on the natural history of this tumour including its metastatic potential due to the lack of large case series. We report a case of myoepithelial carcinoma of minor salivary gland with multiple skeletal metastasis. © 2015 The British Association of Oral Surgeons and John Wiley & Sons Ltd. Source

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