Akutagawa I.,Tokyo University of Agriculture and Technology |
Akiyama Y.,Osaka University |
Takahashi Y.,MS Solutions Co. |
Iijima M.,Yokohama National University |
And 3 more authors.
Bioscience, Biotechnology and Biochemistry | Year: 2014
Continuous liquid chromatography-mass spectrometry (LC-MS) analysis was successfully demonstrated by using magnetic TiO2/Fe3O4microparticles at the desalination interface. The particles could be prepared easily even on a practical scale at sufficient quality for efficient phosphate adsorption. Not only phosphate but several biomolecules were adsorbed onto the particles in a non-specific manner. Such samples could still be detected effectively in MS because the removal of phosphate derived from the LC eluent enhanced sample ionization and resulted in a significant reduction of phosphate cluster ions. © 2014 Japan Society for Bioscience, Biotechnology, and Agrochemistry.
Masada-Atsumi S.,Japan National Institute of Health Sciences |
Kumeta Y.,Japan National Institute of Health Sciences |
Takahashi Y.,MS Solutions Co. |
Hakamatsuka T.,Japan National Institute of Health Sciences |
Goda Y.,Japan National Institute of Health Sciences
Biological and Pharmaceutical Bulletin | Year: 2014
Despite the increasing sales of black cohosh (the dried rhizome and root of Cimicifuga racemosa L.) in the world herbal market, these products have continuous adulteration issues. The botanical authenticity of the black cohosh products is the first important step for ensuring their quality, safety and efficacy. In this study, we genetically identified the botanical sources of 10 black cohosh products and 5 Cimicifuga Rhizome crude drugs of Japanese Pharmacopoeia grade, and analyzed the metabolic profiling of 25 black cohosh products using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Consequently, we found that C. dahurica and possibly C. foetida are misused as sources of the black cohosh products and in some cases, the extracts of black cohosh were adulterated with the plant materials of C. dahurica. We demonstrated that these three species can be distinguished by three marker compounds in a specific mass range. These results must be helpful in establishing regulations for the safe use of the black cohosh products. © 2014 The Pharmaceutical Society of Japan.
Yahagi T.,International University of Health and Welfare |
Yahagi T.,Nihon University |
Masada S.,Japan National Institute of Health Sciences |
Oshima N.,International University of Health and Welfare |
And 11 more authors.
Chemical and Pharmaceutical Bulletin | Year: 2016
Shrub Chaste Tree Fruit (SCTF) is defined as the fruits of Vitex rotundifolia L. f. and V. trifolia L. and has been used as a component of some traditional Japanese medicines (Kampo formulations). Agnus Castus Fruit (ACF) is defined as the dried ripe fruits of V. agnus-castus L.; it is used in traditional European medicines, but is becoming popular in Japan as both an over-the-counter drug and as an ingredient in health foods for treating premenstrual syndrome (PMS). To ensure the efficacy and safety of both SCTF and ACF products, it is important to precisely authenticate their botanical origins and to clearly distinguish between SCTF and ACF. Therefore, we tried to identify SCTF-specific marker compounds based on LC/MS metabolic analysis. The multivariate analysis of LC/MS data from SCTF and ACF samples furnished candidate marker compounds of SCTF. An SCTF-specific marker was isolated from SCTF crude drugs and identified as 3-O-trans-feruloyl tormentic acid on the basis of spectroscopic data from NMR and MS. Since avoiding contamination from closely related species is a significant requirement for pharmaceuticals of natural origin, this information will be valuable for the quality control of both SCTF and ACF products from the viewpoint of regulatory science. © 2016 The Pharmaceutical Society of Japan.
Akiyama Y.,JEOL Ltd. |
Akiyama Y.,Tokyo University of Agriculture and Technology |
Akiyama Y.,MS Solutions Co. |
Takahashi Y.,JEOL Ltd. |
And 4 more authors.
International Journal of Mass Spectrometry | Year: 2011
Phosphate buffer used commonly in liquid chromatography (LC) separation is not appropriate for mass spectrometry (MS) with an electrospray ionization source because nonvolatile salts in the buffer prevent sample molecules from being ionized. We have newly developed a desalination interface device (DID) for LC-MS using the specific adsorption of phosphate onto TiO2-coated magnetic microparticles (TCMMPs). The TCMMPs which adsorb phosphate onto their own surface are collected by magnetic force in the microchannel of the DID and removed. The DID successfully collected almost all TCMMPs and the remaining phosphate rate was 15.1%. The signal-to-noise (S/N) ratio in the extracted ion chromatogram of reserpine obtained by LC-DID-MS was increased 5.3-fold compared to the one obtained by LC-MS. Our results indicate that the DID should be a powerful tool allowing phosphate buffer use in an on-line LC-MS system. © 2011 Elsevier B.V. All rights reserved.
Fuchino H.,Japan National Institute of Biomedical Innovation |
Daikonya A.,Japan National Institute of Biomedical Innovation |
Kumagai T.,Japan National Institute of Biomedical Innovation |
Goda Y.,Japan National Institute of Health Sciences |
And 2 more authors.
Chemical and Pharmaceutical Bulletin | Year: 2013
Degradation of the components of Leonurus Herb was examined during drying. Compounds 1 and 2 were detected on TLC at lower temperature, but not at higher temperature. Their chemical structures were determined by spectral methods. They immediately decomposed even at 40°C in chloroform solution. They are believed to be transformed through a retro-aldol reaction. Compounds 1 and 2 have not been previously reported. © 2013 The Pharmaceutical Society of Japan.