MR Government Arts College

Mannargudi, India

MR Government Arts College

Mannargudi, India
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Kredics L.,University of Szeged | Narendran V.,Aravind Eye Hospital and Postgraduate Institute of Ophthalmology | Shobana C.S.,PSG College of Technology | Vagvolgyi C.,University of Szeged | And 19 more authors.
Mycoses | Year: 2015

Fungal keratitis is a serious suppurative, usually ulcerative corneal infection which may result in blindness or reduced vision. Epidemiological studies indicate that the occurrence of fungal keratitis is higher in warm, humid regions with agricultural economy. The most frequent filamentous fungal genera among the causal agents are Fusarium, Aspergillus and Curvularia. A more successful therapy of fungal keratitis relies on precise identification of the pathogen to the species level using molecular tools. As the sequence analysis of the internal transcribed spacer (ITS) region of the ribosomal RNA gene cluster (rDNA) is not discriminative enough to reveal a species-level diagnosis for several filamentous fungal species highly relevant in keratitis infections, analysis of other loci is also required for an exact diagnosis. Molecular identifications may also reveal the involvement of fungal species which were not previously reported from corneal infections. The routinely applied chemotherapy of fungal keratitis is based on the topical and systemic administration of polyenes and azole compounds. Antifungal susceptibility testing of the causal agents is of special importance due to the emergence and spread of resistance. Testing the applicability of further available antifungals and screening for new, potential compounds for the therapy of fungal keratitis are of highlighted interest. © 2015 Blackwell Verlag GmbH.

Panneer Selvam K.,Mr Government Arts College | Annamalai K.,Bharathiar University | Nambirajan G.,Mr Government Arts College
International Journal of Pharma and Bio Sciences | Year: 2014

Indolicidin is an antimicrobial cationic peptide naturally present in the bovine neutrophils. In this study, transformed Escherichia coli cells were employed for greater and cost effective production of indolicidin. Four strains of E. coli [C41 (DE3), C43 (DE3), C41 (DE3) pLysS & C43 (DE3) pLysS] were transformed with recombinant plasmid pet21a+ carrying the indolicidin gene and were successfully expressed for indolicidin in the presence of the inducer isopropyl β-D-1-thiogalactopyranoside (IPTG). Further, the production of indolicidin was confirmed by detecting the 2 KDa indolicidin band on sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE).

Sundar S.K.,Mr Government Arts College | Manavalan M.,Coordinator Center for Biological science
Journal of Applied Pharmaceutical Science | Year: 2014

Approximately 1 in 3 women will require antimicrobial treatment for a urinary tract infection (UTI). Forty to fifty percentage of the woman will have a UTI before the age of twenty four during their life time. UTI'S in male patients are rare but once infected considered to be complicated. Infection of urinary tract is amongst the most common bacterial infections that prompt patient's to seek medical advice second only to infection of respiratory tract In the present study, the isolation of urinary tract infecting pathogens from patients of different gender and age group revealed that Escherichia coli was the dominant isolate followed by Klebsiella, Enterobacter, Pseudomonas, Proteus, Acinetobacter, and Staphylococcus aureus. © 2014 Anusha et al.

Sundar S.K.,Mr Government Arts College | Kumari Pushpa Rani T.P.,Noorul Islam University | Vijayalakshmi B.,Noorul Islam University | Murugan M.,Noorul Islam University
International Journal of Pharma and Bio Sciences | Year: 2014

Acinetobacter is one of the important opportunistic pathogens involved in hospital acquired infection. They cause various types of human infections leading to morbidity and mortality. Of these Acinetobacter species, Acinetobacter baumannii is the most prevalent in clinical specimens causing pneumonia. It is very common among inpatients admitted in ICUs for various ailments. Around hundred sputum samples were collected from patients visiting various hospitals of Kanyakumari District, Tamil Nadu. From this clinical specimen, 16 were identified preliminarily as Acinetobacter baumannii by various morphological and biochemical tests. The identities of the bacterial isolates were confirmed by 16S rRNA sequence analyses. Antibiotic susceptibility testing was performed by the agar disc diffusion method. Variations were observed in the resistance pattern of the bacterial isolates towards various commercial antibiotics. The genetic variability of Acinetobacter baumannii isolates were detected using RAPD assay. The RAPD assay using different primers showed distinct molecular variation among the 16 isolates suggesting the emergence of new resistant strains among the clinical isolates.

Rajaduraipandi K.,The Erode Diagnostic Laboratory | Morrison D.,Stobhill Hospital | Mani M.,Microbiological Laboratory | Nambirajan G.,Mr Government Arts College | And 5 more authors.
Acta Biologica Szegediensis | Year: 2014

MRSA infection is alarming particularly in hospital set ups/community. We typed 43 isolates of Staphylococcus aureus (MRSA and MSSA) based on genomic DNA restriction fragment length polymorphisms (RFLPs). The genomic DNA of the test isolates was digested with SmaI enzyme, fractionated by PFGE and the patterns were assessed by dendrogram for percentage similarity. The SmaI restricted genomic DNA of 19 MRSA and 24 MSSA identified 27 different PFGE patterns, in which 11 and 16 were from MRSA and MSSA, respectively. Prevalence predominance was observed in few types/subtypes of MSSA (type B and subtype I-1) and MRSA (sub type A-2) and high percentage of similarity was noticed among the subtypes of PFGE types such as P and I of MSSA. During the epidemiological studies, to understand the dissemination of endemic/epidemic MRSA and MSSA, PFGE-based typing of pathogens may be used as a reliable and effective typing method.

Beula Rani K.R.,Alagappa Chettiar College of Engineering And Technology | Sundar S.K.,Mr Government Arts College | Murugan M.,Center for Biological Science
Asian Journal of Pharmaceutical and Clinical Research | Year: 2016

Objective: The study was undertaken to evaluate the antimicrobial activity and phytochemical constituents of the medicinal plant Alpinia galanga. Methods: The plant samples were collected from the places of Mandaikadu, Kanyakumari District, Tamil Nadu, India. Plant extracts were prepared from the dried leaf powder using different solvents include acetone, chloroform, diethyl ether, ethanol, and distilled water. Initially, antimicrobial activities of the plant extracts were tested by agar well diffusion method against four bacterial, viz., Escherichia coli, Klebsiella pneumonia, Bacillus cereus, and Staphylococcus aureus and two fungal pathogens, viz., Aspergillus niger and Penicillium chrysogenum. Further, the plant extracts were screened for phytochemical constituents by standard protocols. Results: In this study, the entire bacterial pathogens have inhibited by acetone, chloroform, diethyl ether, and ethanol extracts. In phytochemical screening tests, the plant extracts showed positive results for carbohydrates, amino acid, chloride, alkaloids, flavonoids, phenols, and terpenoids. Conclusion: This study reveals the continuation of antimicrobial components in the medicinal plant A. galanga. © 2016, Innovare Academics Sciences Pvt. Ltd. All rights reserved.

Balakumar S.,SASTRA University | Rajan S.,Mr Government Arts College | Thirunalasundari T.,Bharathidasan University | Jeeva S.,Center for Biodiversity and Biotechnology
Asian Pacific Journal of Tropical Medicine | Year: 2011

Objective: To assess antifungal activity of Ocimum sanctum leaves against dermatophytic fungi. Methods: Antifungal activity of Ocimum sanctum leaves was measured by 38 A NCCLS method. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of various extracts and fractions of Ocimum sanctum leaves were also determined. Results: Ocimum sanctum leaves possessed antifungal activity against clinically isolated dermatophytes at the concentration of 200 μg/mL. MIC and MFC were high with water fraction (200 μg/mL) against dermatophytic fungi used. Conclusions: Ocimum sanctum has antifungal activity, and the leaf extracts may be a useful source for dermatophytic infections. © 2011 Hainan Medical College.

Gurulakshmi M.,Anna University | Selvaraj M.,Anna University | Selvamani A.,Anna University | Vijayan P.,Mr Government Arts College | And 2 more authors.
Applied Catalysis A: General | Year: 2012

Diverse approaches are being made in search of a visible-light active TiO2 photocatalysts. In this study, S-TiO2(x) nanocatalysts with x = 2, 4 and 6 (x = S/Ti molar ratio) were synthesized by sol-gel method and V2O5/S-TiO2(x) nanocomposites by wet impregnation method using ammonium metavanadate as a source of vanadium. The synthesized catalysts were characterized by diffuse reflectance UV-vis spectroscopy, X-ray diffraction, Raman spectroscopy, N 2 adsorption desorption studies, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy and high resolution transmission electron microscopy techniques. The photogenerated reactive species OH radical estimated from photoluminescence spectra was directly correlated to the visible-light absorption of the catalyst. The photocatalytic activities of all the synthesized catalysts were evaluated for the degradation of MB under visible light irradiation. The excellent stability of V2O 5/S-TiO2(x) nanocomposites has been revealed from the linear relationship between the fluorescence intensity and the time. The addition of sulfur and V2O5 to TiO2 has significantly enhanced the visible-light photocatalytic activity by modifying the optical and electronic properties of TiO2. The enhanced visible-light photocatalytic activity has been attributed to the efficient visible-light absorption of V2O5/S-TiO2(x) nanocomposites and efficient charge separation due to migration of charge carriers between S-TiO2 and V2O5. © 2012 Elsevier B.V.

Vigneshwaran A.N.,Government Arts College Autonomous | Paramasivam P.,Mr Government Arts College | Raja C.R.,Government Arts College Autonomous
Materials Letters | Year: 2016

In this letter, we report on the linear and nonlinear optical investigations of 5 mol% of Li, NH4 doped potassium pentaborate (KB5) crystal. Doping concentration was quantified by the EDAX and ICP analysis. The enhancement of transparency in the doped crystals was recorded in UV-vis-NIR spectra. Optical band gap of the pure and doped crystals was also calculated. The optical nonlinearity of the grown crystals was confirmed by Second Harmonic Generation (SHG) test which shows that the dopants in the KB5 crystal enhanced the SHG efficiency of that material. The third order nonlinearity was studied by Z- scan technique using 632.8 nm He-Ne laser. The values of nonlinear absorption coefficient (β) and nonlinear refractive index (n2) were calculated. Third order nonlinear susceptibility (χ(3)) of the Li and NH4 doped crystals is 1.0278×10-3 esu and 0.7744×10-3 esu respectively which is higher than that of pure KB5 (0.6770×10-3 esu). © 2016 Elsevier B.V.

Sundar S.K.,Mr Government Arts College | Anusha S.U.,Mr Government Arts College
International Journal of Pharma and Bio Sciences | Year: 2015

The background of the present Insilico studies, was to find out the potential protein target in Eae iuntimin (E coli) and deliver the 3 D structure and to find out the best inhibitors of the virulence protein. Alkaloids from medicinally important plant Phyllanthus emblica (Phyllantidine and Phyllanthine) were used for the above purpose using molecular drug docking techniques. The Phyllanthine, Phyllantidine and the commercial antibiotic gentamicine potentially inhibited the target protein Eae iuntimin (E coli). Based on the molecular drug docking and binding affinities of the target protein Eae iuntimin (E coli) it was found that the Amla alkaloid Phyllanthine showed high binding values than Phyllantidine and the existing drug Gentamicine.

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