Mother Theresa Institute of Health science

Puducherry, India

Mother Theresa Institute of Health science

Puducherry, India
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Ashok Kumar J.,Prist University | Sathya A.,SASTRA University | Patil Sagar N.,Prist University | Prathap B.,Prist University | And 2 more authors.
International Journal of Research in Pharmaceutical Sciences | Year: 2010

A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was devel- oped for simultaneous estimation of olmesartan medoxomil (OLM) and hydrochlorothiazide (HCTZ) in combined tablet dosage form. Formulation containing OLM with HCTZ are used as antihypertensive angiotensin II receptor blocker. Chromatography was performed on a 250 mm x 4.6 mm, 5-μm particle size, C8 Qualisil BDS column with a 50:50 (v/v) mixture of buffer and acetonitrile as a mobile phase and the pH was adjusted to 4.7 by adding dilute phosphoric acid. The detection of the combined dosage form was carried out at 225 nm and a flow rate employed was 1 ml min-1. The retention times were 5.074 & 7.242 min for olmesartan medoxomil and hydrochlorothiazide, respectively. Linearity was obtained in the concentration range 20 to 100 μg mL-1 for olmesartan medoxomil and in the range 12.5 to 62.5 μg mL-1 for hydrochlorothiazide, with a correlation coefficient of 0.9956 and 0.989.The result of the analysis were validated statistically and recovery studies confirmed the accuracy and precision of the proposed method. © Pharmascope Foundation.

Kumar J.A.,PRIST University | Pullakandam N.,PRIST University | Prabu S.L.,Anna University | Gopal V.,Mother Theresa Institute of Health science
International Journal of Pharmaceutical Sciences Review and Research | Year: 2010

Today about 74% of drugs are taken orally and are found not to be as effective as desired. To improve such characters transdermal drug delivery system was emerged. Drug delivery through the skin to achieve a systemic effect of a drug is commonly known as transdermal drug delivery and differs from traditional topical drug delivery. Transdermal drug delivery systems (TDDS) are dosage forms involves drug transport to viable epidermal and or dermal tissues of the skin for local therapeutic effect while a very major fraction of drug is transported into the systemic blood circulation. The adhesive of the transdermal drug delivery system is critical to the safety, efficacy and quality of the product. Topical administration of therapeutic agents offers many advantages over conventional oral and invasive methods of drug delivery. Several important advantages of transdermal drug delivery are limitation of hepatic first pass metabolism, enhancement of therapeutic efficiency and maintenance of steady plasma level of the drug. This article provides an overview of types of Transdermal patches, methods of preparation and its physicochemical methods of evaluation.

Ashok Kumar J.,Tamil University | Gopal V.,Mother Theresa Institute of Health science
International Journal of Research in Pharmaceutical Sciences | Year: 2013

Transdermal drug delivery system of nano carvedilol has been formulated by solvent casting method. Nano sized carvedilol was prepared by high pressure homogenization technique and morphology of nano particles confirmed by scanning electron microscopy. Sustained release nano carvedilol matrix patches were prepared by using hy-droxyl propyl methyl cellulose (HPMC) and poly vinyl pyrrolidone (PVP). Physicochemical parameters like thickness uniformity, folding endurance, elongation break, weight variation, drug content uniformity, percentage moisture content, percentage moisture uptake, water vapor transmission rate (WVTR) were evaluated. On the basis of in vitro drug release studies (12 hours) and ex vivo permeation performance, formulation code CT2 was found to be better than other formulation and it was selected as the optimized formulation. In vitro kinetic studies results followed higuchi (r2=0.9948), and the mechanism of release was diffusion. The optimized patch was found to be stable at 40°C ± 2°C and 75 ± 5% RH for six month with respect to their physicochemical parameters and drug content. © JK Welfare & Pharmascope Foundation.

Ashok Kumar J.,University University | Rajesh M.,University University | Gopal V.,Mother Theresa Institute of Health science
Journal of Pharmaceutical Research and Health Care | Year: 2011

A gel forming Polysaccharide gum obtained form the bark of Araucaria bidwilli was employed as a matrix sustained release tablet formulation of Diclofenac sodium (a non steroidal anti inflammatory agent). The effect of Araucaria bidwilli gum (Natural) and Synthetic polymer Hydroxypropyl methyl cellulose (HPMC K4 M) on the release of Diclofenac sodium was studied. The FT-IR spectroscopic studies of drug, gum and mixture indicated no chemical interaction. Six formulations were prepared by wet granulation method containing Araucaria bidwilli gum powder concentration 10% 20% & 30% w\w and 10% 20% &30% w\w of HPMC K4 M with sufficient volume of granulating agent Polyvinyl pyrrolene (PVP K 30), Avicel pH101 as diluents, Magnesium stearate and Aerosil is used lubricant and glidant respectively.This study was carried out to find out the difference between synthetic and natural gum and whether synthetic gum can be replaced by natural gums. Physical and technological studies of granules and tablets were compliance with Pharmacopoial standards.The drug release increased with Araucaria bidwilli gum when compared to synthetics polymer concentration.The value of release exponent were found to be almost straight line and regression coefficient value between 0.938 and 0.998.This implies that the release mechanism is diffusion. Formulation F3 (contained 30% w\w Araucaria bidwilli gum) met the desired requirements for a sustained release dosage form.

Gopal V.,Mother Theresa Institute of Health science
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2010

A polyherbal formulation is investigated for hepatoprotective activity against carbon tetrachloride induced hepatic damage in female albino Wister rats. The hepatoprotective activity of polyherbal formulation is compared with the standard drug Liv-52.The polyherbal formulation has shown significant hepatoprotective activity by reducing the elevated levels of serum enzymes such as serum glutamate oxaloacetate transaminase(SGOT) to 86.96 IU/L, serum glutamate pyruvate transaminase(SGPT) to 47.62 IU/L, alkaline phosphate(ALP) to 446 IU/L, Bilirubin level to 0.57 mg/dl and significant increase in total protein and albumin 9.5 g/dl and 7.74 g/dl respectively when compared to the standard drug Liv-52 which decreased SGOT to 89.40U/LSGPT to 49.49 IU/L, ALKP to 509 IU/L, Bilirubin level to 0.57 mg/dl and increased total protein and albumin levels to 9.5 gm/dl and 7.14 gm/dl respectively against carbon tetrachloride intoxicated rats in comparison to normal control. These biochemical observations were also supplemented by histopathological examinations of the liver sections. The results showed that polyherbal formulation was most active.

Kavimani S.,Mother Theresa Institute of Health science | Shakil Sait S.,Dr. Reddys laboratories Pvt. Ltd
Der Pharmacia Lettre | Year: 2013

Food-drug interaction is a challenging concept, since the consumption of food and other herbal drugs is not documented in patient's profile. With this aspect the present study was designed to investigate the possible effect of Pineapple juice on glimepiride (substrate for CYP2C9) an oral hypoglycemic drug in wistar albino rats. Relative to the standard group (glimepiride 1mg/kg alone treated), the reduction of blood glucose level of test group (Pineapple juice 3mL+glimepiride 1mg/kg treated) was more in both acute and sub-acute in alloxan induced diabetic rats when Pineapple juice (3 mL) was injected p.o.1 h before the p.o. administration of the glimepiride (1mg/kg). These results suggest Pineapple juice ingestion increases the efficacy of glimepiride may be by inhibiting intestinal CYP2C9 enzyme in albino wistar rats.

Kavimani S.,Mother Theresa Institute of Health science
International Journal of Pharmaceutical Sciences Review and Research | Year: 2011

Extended release formulation of metformin hydrochloride presents significant challenges due to its poor inherent compressibility, high dose and high water solubility. Extended release matrix tablets of metformin hydrochloride were formulated different combination of polymers in hydroxyl propyl methyl cellulose (K100M) and ethyl cellulose (18 centipoise) (DRUG: HPMC: EC in the ratios of Formulations F1 5:1:1:1, F2 5:1.5:1.5 and F3 5:2:2 respectively by direct compression method. The formulated powder blends were evaluated for compatibility (DSC), angle of repose, True density, bulk density, compressibility index and total porosity. The tablets were subjected to thickness, weight variation test, hardness test, friability test and drug content test. In-vitro release studies were carried out at pH 1.2 simulated gastric fluids for first 2h and followed by simulated intestinal fluid at pH 7.2 using the apparatus (basket) equipment as described in the USP dissolution monograph. The formulated powder blends showed satisfactory flow properties and drug content. The selected formulation further subjected to accelerated stability studies up to 12 and 6 months as per ICH guidelines at room and accelerated temperature and in-vitro and in-vivo release studies carried out formulation F3 in Wistar albino rats to find out the reduction of blood glucose level using blood glucometer up to 10h. Tablet thus formulated provided extended release of metformin hydrochloride over a period of 12 h. Formulation F3 was selected on the basis of t25, t50 and t90 using ANOVA, paired t-test pharmacokinetic studies and compared with reference standard (marketed sustained release tablet) (F4M).

Patil J.,PRIST University | Kadam C.,Mother Theresa Institute of Health science | Vishwajith V.,Mother Theresa Institute of Health science | Gopal V.,PRIST University | Gopal V.,Mother Theresa Institute of Health science
International Journal of Pharma and Bio Sciences | Year: 2011

The present work aims to formulate and design orally disintegrating tablets containing antihistamines like Loratadine using different pharmaceutical compositions with simple manufacturing procedures to enhance patient compliance. Loratadine was formulated into orally disintegrating tablets by direct compression method using suitable excipients like Maltodextrin, Mannitol, Micro crystalline cellulose, combination of Mannitol with Starch, Croscarmellose sodium, Citric acid, Sodium bicarbonate along with Mint flavor, which were evaluated by using simple analytical techniques. The taste of the formulation was enhanced using artificial sweetener Aspartame and Mint flavour. The tablets were evaluated for weight variation, hardness, friability, drug content, wetting time and disintegration time along with invitro dissolution. It was observed that the direct compression process using commercial grades of excipients like combination of Mannitol with Starch and Micro crystalline cellulose as directly compressible diluents along with super disintegrants like Croscarmellose Sodium was found to be more promising to prepare orally disintegrating tablets.

Kayarohanam S.,Jawaharlal Nehru University | Kavimani S.,Mother Theresa Institute of Health science
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2015

Objective: The present study was to evaluate the oral toxicity of acute and sub acute studies of methanol leaf and bark extract of Dolichandrone atrovirens Methods: In acute toxicity studies of aqueous methanolic Dolichandrone atrovirens leaf extract(DALE) and Dolichandrone atrovirens bark extracts (DABE) (in 0.3% sodium CMC) as a single dose (2000 mg/kg) was administered to the Swiss albino mice (20-25 g) by oral route and the animals were observed for mortality and any toxic symptoms up to 14 days. In sub acute toxicity studies the DALE and DABE were administered daily for 28 days at doses ranging from 200-400 mg/kg. The animals were found in signs of toxicity, morbidity and mortality for 28 days. The animals were submitted to observe the serum biochemical markers and weight of the vital organs. Results: The results of 14 days acute toxicity studies up to a dose of 2000 mg/kg of the aqueous methanolic DABE and DALE neither produced mortality nor shows any symptoms of behavior or any physiological changes in body weight, food and water intake. 28 days sub acute studies repeated doses of oral toxicity did not show any toxic signs or any mortality when three doses 200 and 400 mg/kg of the methanolic leaf and bark extracts of Dolichandrone atrovirens administered. No significant changes were integrated in biochemical and hematological parameters when compared with the control group. Conclusion: From the results it is concluded that the dose at 400 mg/kg is safe for long term treatment in diabetic conditions. © 2015, International Journal of Pharmacy and Pharmaceutical Sciences. All Rights Reserved.

Sangeetha M.,Sri Ramachandra University | Chamundeeswari D.,Sri Ramachandra University | Saravana Babu C.,Sri Ramachandra University | Rose C.,CSIR - Central Leather Research Institute | Gopal V.,Mother Theresa institute of Health science
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2013

Albizia procera belongs to the family mimosaceae, found in the sub-Himalayan tracts from Yamuna eastwards to west Bengal, Gujarat, South India and in the Andamans, Madagascar which is used in the treatment of ulcer, cancer, stomach andintestinal diseases and rheumatism. The qualitative phyto-chemical screening showed the presence of phenols, steroids, alkaloids, flavanoids, tannins, carbohydrates, terpenoids, saponins and proteins. The Petroleum ether, Chloroform, Ethyl acetate, Ethanol and Hydro alcohol extracts of the bark of Albizia procera were subjected to In vitro Anti- Inflammatoryactivity by HRBC membrane stabilization method and In vitro antioxidant activity by Lipid Per Oxidation method in various concentrations i.e. 10, 50, 100, 200, 400, 800, 1000 μg/ml. In Anti-Inflammatory activity the effect was represented as follows Ethanol> Ethyl acetate > Hydro alcohol > Chloroform>Petroleum ether. In Lipid per oxidation activity, the effect was represented as follows Ethanol > Hydro alcohol> Ethyl acetate > Chloroform > Petroleum ether Albizia procera exhibits the above activity which can be accredited by phenolic acids present in it.

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