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Young J.W.,California Institute of Technology | Locke J.C.W.,California Institute of Technology | Altinok A.,California Institute of Technology | Rosenfeld N.,Cancer Research UK Research Institute | And 4 more authors.
Nature Protocols | Year: 2012

Quantitative single-cell time-lapse microscopy is a powerful method for analyzing gene circuit dynamics and heterogeneous cell behavior. We describe the application of this method to imaging bacteria by using an automated microscopy system. This protocol has been used to analyze sporulation and competence differentiation in Bacillus subtilis, and to quantify gene regulation and its fluctuations in individual Escherichia coli cells. The protocol involves seeding and growing bacteria on small agarose pads and imaging the resulting microcolonies. Images are then reviewed and analyzed using our laboratory's custom MATLAatlaB analysis code, which segments and tracks cells in a frame-to-frame method. process yields quantitative expression data on cell lineages, which can illustrate dynamic expression profiles and facilitate mathematical models of gene circuits. With fast-growing bacteria, such as E. coli or B. subtilis, image acquisition can be completed in 1 d, with an additional 1-2 d for progressing through the analysis procedure. © 2012 Nature America, Inc. All rights reserved.


Adaskin A.M.,Moscow State Technological University “Stankin“
Metal Science and Heat Treatment | Year: 2012

It is shown that it is possible in principle to change the shape of articles from steels and alloys with low ductility without changing their mechanical properties due to the relaxation of stresses occurring in the range of low-temperature creep. A process for commercial production of springs from cold-worked high-hardness (up to 600 HV) austenitic wire is developed for making articles of specified shape and sizes without worsening the initial high hardness. © 2012 Springer Science+Business Media, Inc.


Porvatov A.N.,Moscow State Technological University “Stankin“
Measurement Techniques | Year: 2011

An analysis of information measurement systems that are utilized in diagnostics of lathe equipment is presented. A method of construction of these types of systems with the use of circuit-based simulation modeling is proposed. © 2011 Springer Science+Business Media, Inc.


Alexey V.,Moscow State Technological University “Stankin“
Advanced Materials Research | Year: 2013

The paper presents the results of the methodology for improving of cutting tools performance based on modifying the surface properties of the tool material in the formation of wear-resistant complexes, substantially modifying its properties. Argued and offers a threecomponent (for carbide tools) and four-component (for HSS tools) system for modifying the surface properties, improves the tool material. This paper describes a method and results of research to note the substantial increase of efficiency of HSS drills and milling and turning tools made of carbide in the processing of structural steel and hard -to-cut superalloy. © (2013) Trans Tech Publications, Switzerland.


Kuzin V.,Moscow State Technological University “Stankin“ | Grigoriev S.,Moscow State Technological University “Stankin“
Applied Mechanics and Materials | Year: 2014

A microstructural model of surface layer of machine elements from a nonuniform material is developed. With using of this model a calculation scheme that includes basic structural elements is created. Each element is characterized by the following properties: density, elastic modulus, thermal conductivity, linear expansion coefficient and Poisson's ratio. A mathematical model of surface layer of machine elements from a sintered nonuniform material is formulated on the basis of solution of two-dimensional heat-conduction and elasticity problems by the finiteelement method. The created algorithms for solution of these problems are used for formation of an automated system for thermal-strength calculations. This system is surrounded with some original techniques which provide the investigation of the stress-strain state of surface layer of machine elements from a sintered nonuniform material. © (2014) Trans Tech Publications, Switzerland.


Grigoriev S.N.,Moscow State Technological University “Stankin“ | Teleshevskii V.I.,Moscow State Technological University “Stankin“
Measurement Techniques | Year: 2011

The problem of building a quality assurance system for computerized integrated manufacturing in machine building is discussed. Fundamental trends in this area require a new statement of the problem as one of information and measurement technology. The concept of observation as applied to the dynamics of complex controlled systems can be used to define the scope of the measurement processes and their interrelations during the shaping of machined pieces. © 2011 Springer Science+Business Media, Inc.


Kapitanov A.V.,Moscow State Technological University “Stankin“
Procedia Engineering | Year: 2016

The article considers the relevant processes of transition from "traditional" types of computer-aided manufacturing, including both large-scale and mass production, to flexible multi-product manufacturing. In this case, the mission to produce different new part lots is assigned to manufacturing systems consisting of machines with rather wide technological capabilities. In this article, the authors propose an original solution to one of the problems specifying the multi-product manufacturing - a rational choice of equipment capable to produce a given range of products in computer-aided manufacturing. The authors analyzed special characteristics of multi-product manufacturing, as well as identified and justified the need for elaborating a method for classification of workpieces and choice of machines for their processing. According to this study, the authors propose a method of developing the technology for multi-products parts' manufacturing. © 2016 The Authors.


Kremnev L.S.,Moscow State Technological University “Stankin“
Technical Physics | Year: 2013

The method developed earlier for separating the components of a merging doublet of X-ray reflection lines is used to demonstrate that low-carbon martensite with a high concentration of carbide-forming components exhibits a high tetragonality c/a < 1. The effect of carbide-forming and non-carbide-forming components of steel on the tetragonality of martensite is considered. © 2013 Pleiades Publishing, Ltd.


Maslov A.R.,Moscow State Technological University “Stankin“
Measurement Techniques | Year: 2012

Results from the development of an algorithm for automatic measurement of the roughness parameters of a treated surface based on vibro-acoustic diagnostics of the cutting process performed on a lathe are presented. © 2012 Springer Science+Business Media New York.


Grishin S.G.,Moscow State Technological University “Stankin“
Measurement Techniques | Year: 2012

The reasons for the occurrence of the polarization component of the measurement error in heterodyne laser interferometer measuring systems are considered. An analysis of this component is given. © 2012 Springer Science+Business Media, Inc.

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