Yakubovskaya R.I.,Hertsen Moscow Research Institute of Oncology |
Bezborodova O.A.,Hertsen Moscow Research Institute of Oncology |
Nemtsova E.R.,Hertsen Moscow Research Institute of Oncology |
Tutykhina I.L.,Russian Academy of Medical Sciences |
And 5 more authors.
Molecular Genetics, Microbiology and Virology
A recombinant pseudoadenovirus nanostructure (RPAN) based on human adenovirus of the 5th serotype that carries the human Lf gene was constructed to develop an effective system of human lactoferrin (Lf) production. It was shown using the model of toxicosis induced by cisplatin (DDP) that RPAN that carries the expressing cassette with a human Lf gene in its genome provides a high rate of Lf gene expression in animal organisms and ensures a high level of expression. The recombinant human Lf expressed in vivo, as does natural one, decreases the intensity of acute toxic reactions caused by DDP. It was shown that RPAN does not stimulate the growth or metastasis of experimental tumors. Moreover, at the early stages, it inhibits the growth of Lewis lung carcinoma (LLC), Ehrlich carcinoma (ELD), and sarcoma S-37. The obtained experimental data are indicative of the good prospects of the further medical-biological and medical study of RPAN and the production of a new, genetically engineered medicine of the new generation on its basis. © 2010 Allerton Press, Inc. Source