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Minkevich N.I.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Rakitina T.V.,RAS Research Center Kurchatov Institute | Bogachuk A.P.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Radchenko V.V.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | And 7 more authors.
Russian Journal of Bioorganic Chemistry | Year: 2012

We have previously shown that, normally, two forms of the pigment epithelium-derived factor (PEDF) with molecular weights of 50 and 45 kDa are present in the Tenon's capsule in equal amounts. These forms represent the full-length protein and a product of restricted proteolysis of PEDF. In persons with myopia, the full-length uncleaved factor was predominantly detected, which correlated with the disturbance of collagen fiber formation. An immunohistochemical study of the Tenon's capsule using polyclonal antibodies to PEDF showed that, in the control group, the factor is localized solely inside fibroblasts, whereas in patients with myopia, PEDF is distributed outside the cell as a halo around destroyed fibroblasts. It was shown in the present work using atomic force microscopy and immunodot assay with antibodies specific to amyloid fibrils that only the full-length PEDF is capable of forming amyloid-like fibrillar structures. The accumulation of fibrils leads to the destruction of fibroblasts and is a cause of changes in the biochemical composition and morphological structure of the Tenon's capsule in myopia. © Pleiades Publishing, Ltd., 2012.

Kuznetsova A.V.,Russian Academy of Sciences | Kurinov A.M.,Russian Academy of Sciences | Chentsova E.V.,Helmholtz Moscow Research Institute of Eye Diseases | Makarov P.V.,Helmholtz Moscow Research Institute of Eye Diseases | Aleksandrova M.A.,Russian Academy of Sciences
Bulletin of Experimental Biology and Medicine | Year: 2015

Human recombinant protein Wnt7a (hrWnt7a) inhibits cell proliferative activity and triggers cell polarization. Although cell polarization process was maintained only over a short time, probably via microenvironmental stimuli, hrWnt7a is involved in the transformation of the retinal pigment epithelium. Analysis of Wnt signaling pathway and its regulation will help to understand the processes in retinal pigment epithelial cells under pathological conditions, which can be useful in developing new generation drugs. © 2015, Springer Science+Business Media New York.

Kiseleva T.N.,Helmholtz Moscow Research Institute of Eye Diseases | Chudin A.V.,Helmholtz Moscow Research Institute of Eye Diseases
Vestnik Rossiiskoi Akademii Meditsinskikh Nauk | Year: 2014

The review presents the most common methods of modeling of retinal ischemia in vitro (chemical ischemia with iodoacetic acid, incubation of the retinal pigment epithelium cells with oligomycin, deprivation of oxygen and glucose) and in vivo (a model with increased intraocular pressure, cerebral artery occlusion, chronic ligation of the carotid arteries, photocoagulation of the retinal vessels, occlusion of the central retinal artery, endothelin-1 administration). Modeling ischemic injury in rats is the most frequently used method in studies, because the blood supply of their eyes is similar to blood flow in the human eyes. Each method has its own advantages and disadvantages. Application of methods depends on the purpose of the experimental study. Currently model of ocular ischemic disease can be obtained easily by injecting vasoconstrictive drug endothelin-1. It is the most widely used method of high intraocular pressure induced ocular ischemic damage similar to glaucoma, occlusion of central retinal artery or ophthalmic artery in human. The development of experimental models of ocular ischemic diseases and detailed investigation of mechanisms of impairment of microcirculation are useful for improve the efficiency of diagnostic and treatment of ischemic diseases of retina and optic nerve. © 2014, Izdatel'stvo Meditsina. All rights reserved.

Chentsova E.V.,Helmholtz Moscow Research Institute of Eye Diseases | Konyushko O.I.,Helmholtz Moscow Research Institute of Eye Diseases | Makarov M.S.,N V Sklifosovskii Research Institute Of Emergency Medical Care | Egorova N.S.,Helmholtz Moscow Research Institute of Eye Diseases | And 2 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2015

We performed morphofunctional analysis of cultured rabbit buccal epithelial cells in the primary culture, during passaging, and upon interaction with collagen substrates. Three different morphological types were identified. It was demonstrated that precursors of rabbit buccal epithelium were characterized by high proliferative activity, retain their biological properties throughout long-term culturing, and adhere to various collagen substrates. © 2015, Springer Science+Business Media New York.

Vishnyakova K.S.,RAS Engelhardt Institute of Molecular Biology | Babizhayev M.A.,Helmholtz Moscow Research Institute of Eye Diseases | Aliper A.M.,Pathwaypharmaceuticals Ltd | Buzdin A.A.,Pathwaypharmaceuticals Ltd | And 3 more authors.
Molecular Biology | Year: 2014

The concentration of the endogenous dipeptide carnosine reaches tens of millimoles in human muscle tissue. Vast data on various carnosine functions have accumulated over more than 100 years of research, the anti-aging effect being thought the most important. The effect of carnosine was studied in cultured cells. Apart from the well-known effects of increasing the Hayflick limit and causing morphological rejuvenation of cells, carnosine was found to stimulate cell divisions in the colony formation assay and to inhibit the cell transition to quiescence. A transcriptome analysis showed that carnosine-induced changes are mainly related to the positive regulation of the cell cycle at all of the levels from the onset of DNA synthesis to chromosome condensation. It was assumed that the cell cycle-stimulating potential underlies the rejuvenating effect of carnosine and that high carnosine concentrations in muscle tissue are necessary for the recovery (regeneration) of muscles after intense exercise apart from their role as a physiological buffer. © Pleiades Publishing, Inc., 2014.

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